Effect of different lectins on the proliferation of goat peripheral blood mononuclear cells

Detalhes bibliográficos
Autor(a) principal: Regis, Lia Fernandes
Data de Publicação: 2002
Outros Autores: Ribeiro, Marcos, Vale, Vera, Paule, Bruno, Nascimento, Ivana Lúcia de Oliveira, Schaer, Robert Eduard, Nascimento, Roberto José Meyer, Freire, Songeli
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/7459
Resumo: Texto completo: acesso restrito. p. 71-73
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spelling Regis, Lia FernandesRibeiro, MarcosVale, VeraPaule, BrunoNascimento, Ivana Lúcia de OliveiraSchaer, Robert EduardNascimento, Roberto José MeyerFreire, SongeliRegis, Lia FernandesRibeiro, MarcosVale, VeraPaule, BrunoNascimento, Ivana Lúcia de OliveiraSchaer, Robert EduardNascimento, Roberto José MeyerFreire, Songeli2012-12-12T15:54:34Z20020921-4488http://www.repositorio.ufba.br/ri/handle/ri/7459v. 46, n. 1Texto completo: acesso restrito. p. 71-73In the present study we determined the effect of the lectins concanavalin A (Con A), phytohemagglutinin (PHA) and pokeweed (PWM), frequently used in cell proliferation assays, on the proliferation of goat peripheral blood mononuclear cells (PBMCs). The use of a standardized Percoll gradient proved to be highly effective for the separation/enrichment of these cells, without damaging the cell membrane. Pokeweed and phytohemagglutinin showed a high stimulating effect, with proliferation rates of 16,753 and 2350 counts/min (cpm), respectively, whereas Con A showed very low stimulation indices (495 cpm), supporting the notion that it should not be used for blast proliferation studies in goats.Submitted by Suelen Reis (suelen_suzane@hotmail.com) on 2012-12-12T15:54:33Z No. of bitstreams: 1 1-s2.0-S0921448802001542-main.pdf: 67025 bytes, checksum: beae57e2ffe0fea64a55aadacab43b9f (MD5)Made available in DSpace on 2012-12-12T15:54:34Z (GMT). No. of bitstreams: 1 1-s2.0-S0921448802001542-main.pdf: 67025 bytes, checksum: beae57e2ffe0fea64a55aadacab43b9f (MD5) Previous issue date: 2002http://dx.doi.org/10.1016/S0921-4488(02)00154-2reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAPWMCon APHAProliferationGoatsEffect of different lectins on the proliferation of goat peripheral blood mononuclear cellsSmall Ruminant Researchinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01enginfo:eu-repo/semantics/openAccessORIGINAL1-s2.0-S0921448802001542-main.pdf1-s2.0-S0921448802001542-main.pdfapplication/pdf67025https://repositorio.ufba.br/bitstream/ri/7459/1/1-s2.0-S0921448802001542-main.pdfbeae57e2ffe0fea64a55aadacab43b9fMD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/7459/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXT1-s2.0-S0921448802001542-main.pdf.txt1-s2.0-S0921448802001542-main.pdf.txtExtracted texttext/plain8394https://repositorio.ufba.br/bitstream/ri/7459/3/1-s2.0-S0921448802001542-main.pdf.txt68207056a51c92cb8074d545ab4d3819MD53ri/74592022-07-05 14:03:07.341oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:07Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Effect of different lectins on the proliferation of goat peripheral blood mononuclear cells
dc.title.alternative.pt_BR.fl_str_mv Small Ruminant Research
title Effect of different lectins on the proliferation of goat peripheral blood mononuclear cells
spellingShingle Effect of different lectins on the proliferation of goat peripheral blood mononuclear cells
Regis, Lia Fernandes
PWM
Con A
PHA
Proliferation
Goats
title_short Effect of different lectins on the proliferation of goat peripheral blood mononuclear cells
title_full Effect of different lectins on the proliferation of goat peripheral blood mononuclear cells
title_fullStr Effect of different lectins on the proliferation of goat peripheral blood mononuclear cells
title_full_unstemmed Effect of different lectins on the proliferation of goat peripheral blood mononuclear cells
title_sort Effect of different lectins on the proliferation of goat peripheral blood mononuclear cells
author Regis, Lia Fernandes
author_facet Regis, Lia Fernandes
Ribeiro, Marcos
Vale, Vera
Paule, Bruno
Nascimento, Ivana Lúcia de Oliveira
Schaer, Robert Eduard
Nascimento, Roberto José Meyer
Freire, Songeli
author_role author
author2 Ribeiro, Marcos
Vale, Vera
Paule, Bruno
Nascimento, Ivana Lúcia de Oliveira
Schaer, Robert Eduard
Nascimento, Roberto José Meyer
Freire, Songeli
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Regis, Lia Fernandes
Ribeiro, Marcos
Vale, Vera
Paule, Bruno
Nascimento, Ivana Lúcia de Oliveira
Schaer, Robert Eduard
Nascimento, Roberto José Meyer
Freire, Songeli
Regis, Lia Fernandes
Ribeiro, Marcos
Vale, Vera
Paule, Bruno
Nascimento, Ivana Lúcia de Oliveira
Schaer, Robert Eduard
Nascimento, Roberto José Meyer
Freire, Songeli
dc.subject.por.fl_str_mv PWM
Con A
PHA
Proliferation
Goats
topic PWM
Con A
PHA
Proliferation
Goats
description Texto completo: acesso restrito. p. 71-73
publishDate 2002
dc.date.issued.fl_str_mv 2002
dc.date.accessioned.fl_str_mv 2012-12-12T15:54:34Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/7459
dc.identifier.issn.none.fl_str_mv 0921-4488
dc.identifier.number.pt_BR.fl_str_mv v. 46, n. 1
identifier_str_mv 0921-4488
v. 46, n. 1
url http://www.repositorio.ufba.br/ri/handle/ri/7459
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1016/S0921-4488(02)00154-2
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
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instname_str Universidade Federal da Bahia (UFBA)
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institution UFBA
reponame_str Repositório Institucional da UFBA
collection Repositório Institucional da UFBA
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