Immunohistochemical characterization of the inflammatory infiltrate in placental chagas’ disease: a qualitative and quantitative analysis

Detalhes bibliográficos
Autor(a) principal: Altemani, Albina M.
Data de Publicação: 2000
Outros Autores: Bittencourt, Achilea Candida Lisboa, Lana, Ana Maria A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/12795
Resumo: p. 319–324
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spelling Altemani, Albina M.Bittencourt, Achilea Candida LisboaLana, Ana Maria A.Altemani, Albina M.Bittencourt, Achilea Candida LisboaLana, Ana Maria A.2013-09-02T15:46:41Z2013-09-02T15:46:41Z2000http://www.repositorio.ufba.br/ri/handle/ri/12795p. 319–324Abstract. Chagas’ disease, a systemic illness endemic to some regions of South America, is caused by the protozoan Trypanosoma cruzi. Transplacental infection may occur during any phase and cause fetal death. This study is the first to characterize the inflammatory cells in chagasic villitis by immunohistochemistry. Paraffin sections of 8 placentas with villitis by T. cruzi (4 live births and 4 stillbirths), as well as 8 control placentas without inflammation, were stained with hematoxylin and eosin, monoclonal antibodies for CD45RO, CD20, CD45RO/OPD4, CD8, HNK1, CD15, MAC387, and CD68 proteins, and a polyclonal antibody for S-100 protein. Quantification of positive cells was performed in 3 different high-power fields. In all cases of chagasic villitis, the inflammatory infiltrate was composed mainly of CD68 macrophages, T lymphocytes, and a few natural killer cells. Among T cells, CD8 cells outnumbered CD4 cells in all placentas (CD4 :CD8 ratios ranged from 0.04 to 0.38). B cells were absent or rare. In stillbirths, villitis was diffuse and severe with numerous T. cruzi, while in live births it was focal with few parasites. Other features that characterized villitis in stillbirths were 1) frequent trophoblastic necrosis, 2) presence of MAC387 macrophages and CD15 granulocytes attached to the sites of trophoblastic necrosis, 3) low CD4 : CD8 ratios in most cases, 4) increased numbers of S-100 positive cells in the villous stroma. In conclusion, CD68 macrophages and CD8 T lymphocytes were the major cell population in villitis caused by T. cruzi. However, the pattern of inflammatory reaction differed between stillbirths and live births and was probably related to the number of parasites in the placental villi.Submitted by Santiago Fabio (fabio.ssantiago@hotmail.com) on 2013-07-30T18:17:14Z No. of bitstreams: 1 44444444444444444444.pdf: 104547 bytes, checksum: fdbcbe609342f0a9daf12596ec09796e (MD5)Approved for entry into archive by Flávia Ferreira(flaviaccf@yahoo.com.br) on 2013-09-02T15:46:41Z (GMT) No. of bitstreams: 1 44444444444444444444.pdf: 104547 bytes, checksum: fdbcbe609342f0a9daf12596ec09796e (MD5)Made available in DSpace on 2013-09-02T15:46:41Z (GMT). No. of bitstreams: 1 44444444444444444444.pdf: 104547 bytes, checksum: fdbcbe609342f0a9daf12596ec09796e (MD5) Previous issue date: 2000Salvadorhttp://www.ajtmh.org/content/62/2/319.full.pdf+htmlreponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAChagas diseaseTrypanosoma cruziFetal deathSouth AmericaImmunohistochemical characterization of the inflammatory infiltrate in placental chagas’ disease: a qualitative and quantitative analysisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleenginfo:eu-repo/semantics/openAccessORIGINAL44444444444444444444.pdf44444444444444444444.pdfapplication/pdf104547https://repositorio.ufba.br/bitstream/ri/12795/1/44444444444444444444.pdffdbcbe609342f0a9daf12596ec09796eMD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/12795/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXT44444444444444444444.pdf.txt44444444444444444444.pdf.txtExtracted texttext/plain26537https://repositorio.ufba.br/bitstream/ri/12795/3/44444444444444444444.pdf.txtf68ef04fafc225d049f1c89f0ec69880MD53ri/127952022-08-08 13:17:26.993oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-08-08T16:17:26Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Immunohistochemical characterization of the inflammatory infiltrate in placental chagas’ disease: a qualitative and quantitative analysis
title Immunohistochemical characterization of the inflammatory infiltrate in placental chagas’ disease: a qualitative and quantitative analysis
spellingShingle Immunohistochemical characterization of the inflammatory infiltrate in placental chagas’ disease: a qualitative and quantitative analysis
Altemani, Albina M.
Chagas disease
Trypanosoma cruzi
Fetal death
South America
title_short Immunohistochemical characterization of the inflammatory infiltrate in placental chagas’ disease: a qualitative and quantitative analysis
title_full Immunohistochemical characterization of the inflammatory infiltrate in placental chagas’ disease: a qualitative and quantitative analysis
title_fullStr Immunohistochemical characterization of the inflammatory infiltrate in placental chagas’ disease: a qualitative and quantitative analysis
title_full_unstemmed Immunohistochemical characterization of the inflammatory infiltrate in placental chagas’ disease: a qualitative and quantitative analysis
title_sort Immunohistochemical characterization of the inflammatory infiltrate in placental chagas’ disease: a qualitative and quantitative analysis
author Altemani, Albina M.
author_facet Altemani, Albina M.
Bittencourt, Achilea Candida Lisboa
Lana, Ana Maria A.
author_role author
author2 Bittencourt, Achilea Candida Lisboa
Lana, Ana Maria A.
author2_role author
author
dc.contributor.author.fl_str_mv Altemani, Albina M.
Bittencourt, Achilea Candida Lisboa
Lana, Ana Maria A.
Altemani, Albina M.
Bittencourt, Achilea Candida Lisboa
Lana, Ana Maria A.
dc.subject.por.fl_str_mv Chagas disease
Trypanosoma cruzi
Fetal death
South America
topic Chagas disease
Trypanosoma cruzi
Fetal death
South America
description p. 319–324
publishDate 2000
dc.date.issued.fl_str_mv 2000
dc.date.accessioned.fl_str_mv 2013-09-02T15:46:41Z
dc.date.available.fl_str_mv 2013-09-02T15:46:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/12795
url http://www.repositorio.ufba.br/ri/handle/ri/12795
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://www.ajtmh.org/content/62/2/319.full.pdf+html
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
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instname_str Universidade Federal da Bahia (UFBA)
instacron_str UFBA
institution UFBA
reponame_str Repositório Institucional da UFBA
collection Repositório Institucional da UFBA
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