Reduction of galectin-3 expression and liver fibrosis after cell therapy in a mouse model of cirrhosis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2012 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFBA |
| Texto Completo: | http://repositorio.ufba.br/ri/handle/ri/16360 |
Resumo: | Texto completo: acesso restrito. p. 339–349 |
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Oliveira, Sheilla Andrade deSouza, Bruno Solano de FreitasBarreto, Elton Pereira SáKaneto, Carla MartinsAlmeida Neto, HélioAzevedo, Carine MachadoGuimarães, Elisalva TeixeiraFreitas, Luiz Antonio Rodrigues deSantos, Ricardo Ribeiro dosSoares, Milena Botelho PereiraOliveira, Sheilla Andrade deSouza, Bruno Solano de FreitasBarreto, Elton Pereira SáKaneto, Carla MartinsAlmeida Neto, HélioAzevedo, Carine MachadoGuimarães, Elisalva TeixeiraFreitas, Luiz Antonio Rodrigues deSantos, Ricardo Ribeiro dosSoares, Milena Botelho Pereira2014-10-09T15:43:28Z20121465-3249http://repositorio.ufba.br/ri/handle/ri/16360v. 14, n. 3Texto completo: acesso restrito. p. 339–349Background aims Cirrhosis, end-stage liver disease, is caused by different mechanisms of injury, associated with persistent inflammation. Galectin-3 is an important regulator of fibrosis that links chronic inflammation to fibrogenesis. We investigated the role of bone marrow cell (BMC) transplantation in chronic inflammation and hepatic fibrosis. Methods Liver cirrhosis was induced by administration of carbon tetrachloride and ethanol to wild-type C57BL/6 or bone marrow chimeric mice. Bone marrow chimeras were generated by lethal irradiation and transplantation with BMC obtained from green fluorescent protein (GFP+ )donors. Wild-type cirrhotic mice were transplanted with BMC without irradiation. Livers from chimeras and cirrhotic transplanted mice were obtained for evaluation of inflammation, fibrosis and regulatory factors [galectin-3, matrix metallopeptidase (MMP)-9, tissue inhibitor of metalloproteinase (TIMP)-1 and transforming growth factor (TGF)-β]. Results The development of cirrhosis was associated with increased expression of galectin-3 by F4/80+ cells and intense migration of BMC to the liver. Furthermore, when transplanted after the establishment of cirrhosis, BMC also migrated to the liver and localized within the fibrous septa. Two months after BMC therapy, cirrhotic mice had a significant reduction in liver fibrosis and expression of type I collagen. We did not find any difference in levels of TGF-β, TIMP-1 and MMP-9 between saline and BMC groups. However, the numbers of inflammatory cells, phagocytes and galectin-3+ cells were markedly lower in the livers of cirrhotic mice treated with BMC. Conclusions Our results demonstrate an important role for BMC in the regulation of liver fibrosis and that transplantation of BMC can accelerate fibrosis regression through modulatory mechanisms.Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2014-08-19T15:06:24Z No. of bitstreams: 1 Antonio Rodrigues de Freitas.pdf: 617438 bytes, checksum: cf6db99b23516a431130f0307e2e0657 (MD5)Approved for entry into archive by Delba Rosa (delba@ufba.br) on 2014-10-09T15:43:27Z (GMT) No. of bitstreams: 1 Antonio Rodrigues de Freitas.pdf: 617438 bytes, checksum: cf6db99b23516a431130f0307e2e0657 (MD5)Made available in DSpace on 2014-10-09T15:43:28Z (GMT). No. of bitstreams: 1 Antonio Rodrigues de Freitas.pdf: 617438 bytes, checksum: cf6db99b23516a431130f0307e2e0657 (MD5) Previous issue date: 2012http://dx.doi.org/ 10.3109/14653249.2011.637668reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAcarbon tetrachloridegalectin-3hepatic cirrhosismicestem cellsReduction of galectin-3 expression and liver fibrosis after cell therapy in a mouse model of cirrhosisCytotherapyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01info:eu-repo/semantics/openAccessengORIGINALAntonio Rodrigues de Freitas.pdfAntonio Rodrigues de Freitas.pdfapplication/pdf617438https://repositorio.ufba.br/bitstream/ri/16360/1/Antonio%20Rodrigues%20de%20Freitas.pdfcf6db99b23516a431130f0307e2e0657MD51LICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/16360/2/license.txtff6eaa8b858ea317fded99f125f5fcd0MD52TEXTAntonio Rodrigues de Freitas.pdf.txtAntonio Rodrigues de Freitas.pdf.txtExtracted texttext/plain42765https://repositorio.ufba.br/bitstream/ri/16360/3/Antonio%20Rodrigues%20de%20Freitas.pdf.txt5b9f3a29e458455512797031791a51dcMD53ri/163602022-07-05 14:03:32.489oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:32Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
| dc.title.pt_BR.fl_str_mv |
Reduction of galectin-3 expression and liver fibrosis after cell therapy in a mouse model of cirrhosis |
| dc.title.alternative.pt_BR.fl_str_mv |
Cytotherapy |
| title |
Reduction of galectin-3 expression and liver fibrosis after cell therapy in a mouse model of cirrhosis |
| spellingShingle |
Reduction of galectin-3 expression and liver fibrosis after cell therapy in a mouse model of cirrhosis Oliveira, Sheilla Andrade de carbon tetrachloride galectin-3 hepatic cirrhosis mice stem cells |
| title_short |
Reduction of galectin-3 expression and liver fibrosis after cell therapy in a mouse model of cirrhosis |
| title_full |
Reduction of galectin-3 expression and liver fibrosis after cell therapy in a mouse model of cirrhosis |
| title_fullStr |
Reduction of galectin-3 expression and liver fibrosis after cell therapy in a mouse model of cirrhosis |
| title_full_unstemmed |
Reduction of galectin-3 expression and liver fibrosis after cell therapy in a mouse model of cirrhosis |
| title_sort |
Reduction of galectin-3 expression and liver fibrosis after cell therapy in a mouse model of cirrhosis |
| author |
Oliveira, Sheilla Andrade de |
| author_facet |
Oliveira, Sheilla Andrade de Souza, Bruno Solano de Freitas Barreto, Elton Pereira Sá Kaneto, Carla Martins Almeida Neto, Hélio Azevedo, Carine Machado Guimarães, Elisalva Teixeira Freitas, Luiz Antonio Rodrigues de Santos, Ricardo Ribeiro dos Soares, Milena Botelho Pereira |
| author_role |
author |
| author2 |
Souza, Bruno Solano de Freitas Barreto, Elton Pereira Sá Kaneto, Carla Martins Almeida Neto, Hélio Azevedo, Carine Machado Guimarães, Elisalva Teixeira Freitas, Luiz Antonio Rodrigues de Santos, Ricardo Ribeiro dos Soares, Milena Botelho Pereira |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Oliveira, Sheilla Andrade de Souza, Bruno Solano de Freitas Barreto, Elton Pereira Sá Kaneto, Carla Martins Almeida Neto, Hélio Azevedo, Carine Machado Guimarães, Elisalva Teixeira Freitas, Luiz Antonio Rodrigues de Santos, Ricardo Ribeiro dos Soares, Milena Botelho Pereira Oliveira, Sheilla Andrade de Souza, Bruno Solano de Freitas Barreto, Elton Pereira Sá Kaneto, Carla Martins Almeida Neto, Hélio Azevedo, Carine Machado Guimarães, Elisalva Teixeira Freitas, Luiz Antonio Rodrigues de Santos, Ricardo Ribeiro dos Soares, Milena Botelho Pereira |
| dc.subject.por.fl_str_mv |
carbon tetrachloride galectin-3 hepatic cirrhosis mice stem cells |
| topic |
carbon tetrachloride galectin-3 hepatic cirrhosis mice stem cells |
| description |
Texto completo: acesso restrito. p. 339–349 |
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2012 |
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2012 |
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2014-10-09T15:43:28Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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1465-3249 |
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v. 14, n. 3 |
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1465-3249 v. 14, n. 3 |
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http://repositorio.ufba.br/ri/handle/ri/16360 |
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