Enzyme-linked immunosorbent assay for the detection of Bothrops jararaca venom

Detalhes bibliográficos
Autor(a) principal: Barral-Netto, Manoel
Data de Publicação: 1990
Outros Autores: Schriefer, Nicolaus Albert Borges, Vinhas, Vera Silvia de Freitas, Almeida, A. R. Trindade de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/7872
Resumo: Texto completo. Acesso restrito. p. 1 - 9
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spelling Barral-Netto, ManoelSchriefer, Nicolaus Albert BorgesVinhas, Vera Silvia de FreitasAlmeida, A. R. Trindade deBarral-Netto, ManoelSchriefer, Nicolaus Albert BorgesVinhas, Vera Silvia de FreitasAlmeida, A. R. Trindade de2013-01-15T18:54:52Z2013-01-15T18:54:52Z19900041-0101http://www.repositorio.ufba.br/ri/handle/ri/7872v. 28, n. 9Texto completo. Acesso restrito. p. 1 - 9This study reports an enzyme-linked immunosorbent assay for detecting Bothrops jararaca venom in fluids, employing the sandwich method with biotin/avidin amplification. The assay exhibits high accuracy in correlating optical densities with venom concentrations (r = 0.98), high reproducibility, low background and limited cross-reactivity with venom from other snake genera. Nevertheless, it was unable to distinguish among venoms from different bothropic species. Using this method we evaluated the serum kinetics of Bothrops jararaca venom in C57BL/6 mice. High concentrations were found in serum just 15 min after injection (151±41 ng/ml; mean±S.D.), followed by a progressive fall (102±46, 74±39 and 50±22 ng/ml after 1, 3 and 6 hr respectively), being undetectable by 24 hr. Such serum kinetics indicates a pattern of a rapid absorption of venom from the inoculation site, followed by a slow and progressive drop in its serum levels. This ELISA was a reliable tool in the determination of Bothrops jararaca venom levels in mouse serum, and may become useful in other fields of bothropic venom research.Submitted by Santiago Fabio (fabio.ssantiago@hotmail.com) on 2013-01-15T18:54:52Z No. of bitstreams: 1 ccccccccccc.pdf: 526126 bytes, checksum: 164bb2224de40d38cc2b2188124b13c9 (MD5)Made available in DSpace on 2013-01-15T18:54:52Z (GMT). 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dc.title.pt_BR.fl_str_mv Enzyme-linked immunosorbent assay for the detection of Bothrops jararaca venom
dc.title.alternative.pt_BR.fl_str_mv Toxicon
title Enzyme-linked immunosorbent assay for the detection of Bothrops jararaca venom
spellingShingle Enzyme-linked immunosorbent assay for the detection of Bothrops jararaca venom
Barral-Netto, Manoel
title_short Enzyme-linked immunosorbent assay for the detection of Bothrops jararaca venom
title_full Enzyme-linked immunosorbent assay for the detection of Bothrops jararaca venom
title_fullStr Enzyme-linked immunosorbent assay for the detection of Bothrops jararaca venom
title_full_unstemmed Enzyme-linked immunosorbent assay for the detection of Bothrops jararaca venom
title_sort Enzyme-linked immunosorbent assay for the detection of Bothrops jararaca venom
author Barral-Netto, Manoel
author_facet Barral-Netto, Manoel
Schriefer, Nicolaus Albert Borges
Vinhas, Vera Silvia de Freitas
Almeida, A. R. Trindade de
author_role author
author2 Schriefer, Nicolaus Albert Borges
Vinhas, Vera Silvia de Freitas
Almeida, A. R. Trindade de
author2_role author
author
author
dc.contributor.author.fl_str_mv Barral-Netto, Manoel
Schriefer, Nicolaus Albert Borges
Vinhas, Vera Silvia de Freitas
Almeida, A. R. Trindade de
Barral-Netto, Manoel
Schriefer, Nicolaus Albert Borges
Vinhas, Vera Silvia de Freitas
Almeida, A. R. Trindade de
description Texto completo. Acesso restrito. p. 1 - 9
publishDate 1990
dc.date.issued.fl_str_mv 1990
dc.date.accessioned.fl_str_mv 2013-01-15T18:54:52Z
dc.date.available.fl_str_mv 2013-01-15T18:54:52Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/7872
dc.identifier.issn.none.fl_str_mv 0041-0101
dc.identifier.number.pt_BR.fl_str_mv v. 28, n. 9
identifier_str_mv 0041-0101
v. 28, n. 9
url http://www.repositorio.ufba.br/ri/handle/ri/7872
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Toxicon
publisher.none.fl_str_mv Toxicon
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1016/0041-0101(90)90143-U
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
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instname_str Universidade Federal da Bahia (UFBA)
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reponame_str Repositório Institucional da UFBA
collection Repositório Institucional da UFBA
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