Normal expression of IFN-gR in four patients with uncommon mycobacterial infection phenotypes

Detalhes bibliográficos
Autor(a) principal: Rugeles, M.T.
Data de Publicação: 2004
Outros Autores: Rincón, B., Rugeles, C., Montoya, C.J., Hernández, M., Estrada, C., Olivares, M.M., Patiño, P.J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/7150
Resumo: p. 1353-1363.
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spelling Rugeles, M.T.Rincón, B.Rugeles, C.Montoya, C.J.Hernández, M.Estrada, C.Olivares, M.M.Patiño, P.J.Rugeles, M.T.Rincón, B.Rugeles, C.Montoya, C.J.Hernández, M.Estrada, C.Olivares, M.M.Patiño, P.J.2012-11-08T18:11:09Z2012-11-08T18:11:09Z20041414-431Xhttp://www.repositorio.ufba.br/ri/handle/ri/7150v. 37, n. 9p. 1353-1363.Several primary immunodeficiency diseases affecting the interleukin 12/interferon gamma (IFN-g) pathway have been identified, most of them characterized by recurrent and protracted infections produced by intracellular microorganisms, particularly by several species of mycobacteria. In the present study we analyzed the expression of IFN-g receptor (IFN-gR) and signal transducer and activator of transcription 1 (STAT-1) in 4 children with Mycobacterium tuberculosis infection of uncommon clinical presentation. These molecules were evaluated by flow cytometry and Western blotting in B cells transformed with Epstein-Barr virus and mutations were scanned by single-strand conformational polymorphisms and DNA sequencing. The expression of IFN-gR1 was normal in all 4 patients. The genetic analysis of IFN-gR1 and IFN-gR2 coding sequences did not reveal any mutation. The expression of the STAT-1 molecule was similar in patients and healthy controls; however, when the phosphorylation of this transcription factor in response to IFN-g activation was evaluated by Western blot, a significant lower signal was evident in one patient. These data indicate that there are no alterations in the expression or function of the IFN-gR chains in these patients. However, the low level of STAT-1 phosphorylation found in one of these patients might be explained by a defect in one of the molecules involved in the signal transduction pathway after IFN-g interacts with its receptor. In the other three patients the inability to eliminate the mycobacteria may be due to a defect in another effector mechanism of the mononuclear phagocytes.Submitted by JURANDI DE SOUZA SILVA (jssufba@hotmail.com) on 2012-11-08T18:11:09Z No. of bitstreams: 1 5336.pdf: 309980 bytes, checksum: 7b5c759d019f2b2569843611359c150c (MD5)Made available in DSpace on 2012-11-08T18:11:09Z (GMT). No. of bitstreams: 1 5336.pdf: 309980 bytes, checksum: 7b5c759d019f2b2569843611359c150c (MD5) Previous issue date: 2004Ribeirão Pretohttp://dx.doi.org/10.1590/S0100-879X2004000900010reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAMycobacterial diseaseIFN-gIFN-g receptorSTAT-1SSCP-PCRNormal expression of IFN-gR in four patients with uncommon mycobacterial infection phenotypesBrazilian Journal of Medical and Biological Researchinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleenginfo:eu-repo/semantics/openAccessORIGINAL5336.pdf5336.pdfapplication/pdf309980https://repositorio.ufba.br/bitstream/ri/7150/1/5336.pdf7b5c759d019f2b2569843611359c150cMD51LICENSElicense.txtlicense.txttext/plain1809https://repositorio.ufba.br/bitstream/ri/7150/2/license.txt8bb3e3eb871f5854a9ec419f68651b60MD52TEXT5336.pdf.txt5336.pdf.txtExtracted texttext/plain43278https://repositorio.ufba.br/bitstream/ri/7150/3/5336.pdf.txt0e84e02ad4cc773a31e61650d308f8ddMD53ri/71502022-07-05 14:03:09.08oai:repositorio.ufba.br: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ório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:09Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Normal expression of IFN-gR in four patients with uncommon mycobacterial infection phenotypes
dc.title.alternative.pt_BR.fl_str_mv Brazilian Journal of Medical and Biological Research
title Normal expression of IFN-gR in four patients with uncommon mycobacterial infection phenotypes
spellingShingle Normal expression of IFN-gR in four patients with uncommon mycobacterial infection phenotypes
Rugeles, M.T.
Mycobacterial disease
IFN-g
IFN-g receptor
STAT-1
SSCP-PCR
title_short Normal expression of IFN-gR in four patients with uncommon mycobacterial infection phenotypes
title_full Normal expression of IFN-gR in four patients with uncommon mycobacterial infection phenotypes
title_fullStr Normal expression of IFN-gR in four patients with uncommon mycobacterial infection phenotypes
title_full_unstemmed Normal expression of IFN-gR in four patients with uncommon mycobacterial infection phenotypes
title_sort Normal expression of IFN-gR in four patients with uncommon mycobacterial infection phenotypes
author Rugeles, M.T.
author_facet Rugeles, M.T.
Rincón, B.
Rugeles, C.
Montoya, C.J.
Hernández, M.
Estrada, C.
Olivares, M.M.
Patiño, P.J.
author_role author
author2 Rincón, B.
Rugeles, C.
Montoya, C.J.
Hernández, M.
Estrada, C.
Olivares, M.M.
Patiño, P.J.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rugeles, M.T.
Rincón, B.
Rugeles, C.
Montoya, C.J.
Hernández, M.
Estrada, C.
Olivares, M.M.
Patiño, P.J.
Rugeles, M.T.
Rincón, B.
Rugeles, C.
Montoya, C.J.
Hernández, M.
Estrada, C.
Olivares, M.M.
Patiño, P.J.
dc.subject.por.fl_str_mv Mycobacterial disease
IFN-g
IFN-g receptor
STAT-1
SSCP-PCR
topic Mycobacterial disease
IFN-g
IFN-g receptor
STAT-1
SSCP-PCR
description p. 1353-1363.
publishDate 2004
dc.date.issued.fl_str_mv 2004
dc.date.accessioned.fl_str_mv 2012-11-08T18:11:09Z
dc.date.available.fl_str_mv 2012-11-08T18:11:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/7150
dc.identifier.issn.none.fl_str_mv 1414-431X
dc.identifier.number.pt_BR.fl_str_mv v. 37, n. 9
identifier_str_mv 1414-431X
v. 37, n. 9
url http://www.repositorio.ufba.br/ri/handle/ri/7150
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1590/S0100-879X2004000900010
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
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reponame_str Repositório Institucional da UFBA
collection Repositório Institucional da UFBA
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