Normal expression of IFN-gR in four patients with uncommon mycobacterial infection phenotypes
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFBA |
Texto Completo: | http://www.repositorio.ufba.br/ri/handle/ri/7150 |
Resumo: | p. 1353-1363. |
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Rugeles, M.T.Rincón, B.Rugeles, C.Montoya, C.J.Hernández, M.Estrada, C.Olivares, M.M.Patiño, P.J.Rugeles, M.T.Rincón, B.Rugeles, C.Montoya, C.J.Hernández, M.Estrada, C.Olivares, M.M.Patiño, P.J.2012-11-08T18:11:09Z2012-11-08T18:11:09Z20041414-431Xhttp://www.repositorio.ufba.br/ri/handle/ri/7150v. 37, n. 9p. 1353-1363.Several primary immunodeficiency diseases affecting the interleukin 12/interferon gamma (IFN-g) pathway have been identified, most of them characterized by recurrent and protracted infections produced by intracellular microorganisms, particularly by several species of mycobacteria. In the present study we analyzed the expression of IFN-g receptor (IFN-gR) and signal transducer and activator of transcription 1 (STAT-1) in 4 children with Mycobacterium tuberculosis infection of uncommon clinical presentation. These molecules were evaluated by flow cytometry and Western blotting in B cells transformed with Epstein-Barr virus and mutations were scanned by single-strand conformational polymorphisms and DNA sequencing. The expression of IFN-gR1 was normal in all 4 patients. The genetic analysis of IFN-gR1 and IFN-gR2 coding sequences did not reveal any mutation. The expression of the STAT-1 molecule was similar in patients and healthy controls; however, when the phosphorylation of this transcription factor in response to IFN-g activation was evaluated by Western blot, a significant lower signal was evident in one patient. These data indicate that there are no alterations in the expression or function of the IFN-gR chains in these patients. However, the low level of STAT-1 phosphorylation found in one of these patients might be explained by a defect in one of the molecules involved in the signal transduction pathway after IFN-g interacts with its receptor. In the other three patients the inability to eliminate the mycobacteria may be due to a defect in another effector mechanism of the mononuclear phagocytes.Submitted by JURANDI DE SOUZA SILVA (jssufba@hotmail.com) on 2012-11-08T18:11:09Z No. of bitstreams: 1 5336.pdf: 309980 bytes, checksum: 7b5c759d019f2b2569843611359c150c (MD5)Made available in DSpace on 2012-11-08T18:11:09Z (GMT). No. of bitstreams: 1 5336.pdf: 309980 bytes, checksum: 7b5c759d019f2b2569843611359c150c (MD5) Previous issue date: 2004Ribeirão Pretohttp://dx.doi.org/10.1590/S0100-879X2004000900010reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAMycobacterial diseaseIFN-gIFN-g receptorSTAT-1SSCP-PCRNormal expression of IFN-gR in four patients with uncommon mycobacterial infection phenotypesBrazilian Journal of Medical and Biological Researchinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleenginfo:eu-repo/semantics/openAccessORIGINAL5336.pdf5336.pdfapplication/pdf309980https://repositorio.ufba.br/bitstream/ri/7150/1/5336.pdf7b5c759d019f2b2569843611359c150cMD51LICENSElicense.txtlicense.txttext/plain1809https://repositorio.ufba.br/bitstream/ri/7150/2/license.txt8bb3e3eb871f5854a9ec419f68651b60MD52TEXT5336.pdf.txt5336.pdf.txtExtracted texttext/plain43278https://repositorio.ufba.br/bitstream/ri/7150/3/5336.pdf.txt0e84e02ad4cc773a31e61650d308f8ddMD53ri/71502022-07-05 14:03:09.08oai:repositorio.ufba.br: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ório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:09Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
dc.title.pt_BR.fl_str_mv |
Normal expression of IFN-gR in four patients with uncommon mycobacterial infection phenotypes |
dc.title.alternative.pt_BR.fl_str_mv |
Brazilian Journal of Medical and Biological Research |
title |
Normal expression of IFN-gR in four patients with uncommon mycobacterial infection phenotypes |
spellingShingle |
Normal expression of IFN-gR in four patients with uncommon mycobacterial infection phenotypes Rugeles, M.T. Mycobacterial disease IFN-g IFN-g receptor STAT-1 SSCP-PCR |
title_short |
Normal expression of IFN-gR in four patients with uncommon mycobacterial infection phenotypes |
title_full |
Normal expression of IFN-gR in four patients with uncommon mycobacterial infection phenotypes |
title_fullStr |
Normal expression of IFN-gR in four patients with uncommon mycobacterial infection phenotypes |
title_full_unstemmed |
Normal expression of IFN-gR in four patients with uncommon mycobacterial infection phenotypes |
title_sort |
Normal expression of IFN-gR in four patients with uncommon mycobacterial infection phenotypes |
author |
Rugeles, M.T. |
author_facet |
Rugeles, M.T. Rincón, B. Rugeles, C. Montoya, C.J. Hernández, M. Estrada, C. Olivares, M.M. Patiño, P.J. |
author_role |
author |
author2 |
Rincón, B. Rugeles, C. Montoya, C.J. Hernández, M. Estrada, C. Olivares, M.M. Patiño, P.J. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Rugeles, M.T. Rincón, B. Rugeles, C. Montoya, C.J. Hernández, M. Estrada, C. Olivares, M.M. Patiño, P.J. Rugeles, M.T. Rincón, B. Rugeles, C. Montoya, C.J. Hernández, M. Estrada, C. Olivares, M.M. Patiño, P.J. |
dc.subject.por.fl_str_mv |
Mycobacterial disease IFN-g IFN-g receptor STAT-1 SSCP-PCR |
topic |
Mycobacterial disease IFN-g IFN-g receptor STAT-1 SSCP-PCR |
description |
p. 1353-1363. |
publishDate |
2004 |
dc.date.issued.fl_str_mv |
2004 |
dc.date.accessioned.fl_str_mv |
2012-11-08T18:11:09Z |
dc.date.available.fl_str_mv |
2012-11-08T18:11:09Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.repositorio.ufba.br/ri/handle/ri/7150 |
dc.identifier.issn.none.fl_str_mv |
1414-431X |
dc.identifier.number.pt_BR.fl_str_mv |
v. 37, n. 9 |
identifier_str_mv |
1414-431X v. 37, n. 9 |
url |
http://www.repositorio.ufba.br/ri/handle/ri/7150 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.source.pt_BR.fl_str_mv |
http://dx.doi.org/10.1590/S0100-879X2004000900010 |
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reponame:Repositório Institucional da UFBA instname:Universidade Federal da Bahia (UFBA) instacron:UFBA |
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