Dual effect of Lutzomyia longipalpis saliva on Leishmania braziliensis infection is mediated by distinct saliva-induced cellular recruitment into BALB/c mice ear

Detalhes bibliográficos
Autor(a) principal: Carregaro, Vanessa
Data de Publicação: 2013
Outros Autores: Costa, Diego Luis, Brodskyn, Claudia Ida, Barral, Aldina Maria Prado, Barral-Netto, Manoel, Cunha, Fernando Q, Silva, João Santana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/13738
Resumo: p. 1-11
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spelling Carregaro, VanessaCosta, Diego LuisBrodskyn, Claudia IdaBarral, Aldina Maria PradoBarral-Netto, ManoelCunha, Fernando QSilva, João SantanaCarregaro, VanessaCosta, Diego LuisBrodskyn, Claudia IdaBarral, Aldina Maria PradoBarral-Netto, ManoelCunha, Fernando QSilva, João Santana2013-11-19T19:47:17Z2013-11-19T19:47:17Z20131471-2180http://repositorio.ufba.br/ri/handle/ri/13738p. 1-11Background: Leishmania parasites are transmitted to their vertebrate hosts by infected Phlebotomine sand flies during the blood meal of the flies. Sand fly saliva is known to enhance Leishmania spp. infection, while preexposure to saliva protects mice against parasitic infections. In this study, we investigated the initial inflammatory leucocyte composition induced by one or three inocula of salivary gland extract (SGE) from Lutzomyia longipalpis in the presence or absence of Leishmania braziliensis. Results: We demonstrated that inoculating SGE once (SGE-1X) or three times (SGE-3X), which represented a coinoculation or a pre-exposure to saliva, respectively, resulted in different cellular infiltrate profiles. Whereas SGE-1X led to the recruitment of all leucocytes subtypes including CD4+ T cells, CD4+CD25+ T cells, dendritic cells, macrophages and neutrophils, the immune cell profile in the SGE-3X group differed dramatically, as CD4+ T cells, CD4+CD25+ T cells, dendritic cells, macrophages and neutrophils were decreased and CD8+ T cells were increased. The SGE-1X group did not show differences in the ear lesion size; however, the SGE-1X group harbored a higher number of parasites. On the other hand, the SGE-3X group demonstrated a protective effect against parasitic disease, as the parasite burden was lower even in the earlier stages of the infection, a period in which the SGE-1X group presented with larger and more severe lesions. These effects were also reflected in the cytokine profiles of both groups. Whereas the SGE-1X group presented with a substantial increase in IL-10 production, the SGE-3X group showed an increase in IFN-γ production in the draining lymph nodes. Analysis of the inflammatory cell populations present within the ear lesions, the SGE-1X group showed an increase in CD4+FOXP3+ cells, whereas the CD4+FOXP3+ population was reduced in the SGE-3X group. Moreover, CD4+ T cells and CD8+ T cells producing IFN-γ were highly detected in the ears of the SGE-3X mice prior to infection. In addition, upon treatment of SGE-3X mice with anti-IFN-γ monoclonal antibody, we observed a decrease in the protective effect of SGE-3X against L. braziliensis infection. Conclusions: These results indicate that different inocula of Lutzomyia longipalpis salivary gland extract can markedly modify the cellular immune response, which is reflected in the pattern of susceptibility or resistance to Leishmania braziliensis infection.Submitted by Santiago Fabio (fabio.ssantiago@hotmail.com) on 2013-11-18T17:36:44Z No. of bitstreams: 1 1111.pdf: 842320 bytes, checksum: 6965b9264443601e5fa269bef50cd910 (MD5)Approved for entry into archive by Rodrigo Meirelles (rodrigomei@ufba.br) on 2013-11-19T19:47:16Z (GMT) No. of bitstreams: 1 1111.pdf: 842320 bytes, checksum: 6965b9264443601e5fa269bef50cd910 (MD5)Made available in DSpace on 2013-11-19T19:47:17Z (GMT). No. of bitstreams: 1 1111.pdf: 842320 bytes, checksum: 6965b9264443601e5fa269bef50cd910 (MD5) Previous issue date: 2013SalvadorBMC Microbiologyhttp://www.biomedcentral.com/content/pdf/1471-2180-13-102.pdfreponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAPhlebotomines salivaLutzomyia longipalpis salivaLeishmania braziliensisInflammatory leucocytesCytokinesImmunoregulationDual effect of Lutzomyia longipalpis saliva on Leishmania braziliensis infection is mediated by distinct saliva-induced cellular recruitment into BALB/c mice earBMC Microbiologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessengORIGINAL1111.pdf1111.pdfapplication/pdf842320https://repositorio.ufba.br/bitstream/ri/13738/1/1111.pdf6965b9264443601e5fa269bef50cd910MD51LICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/13738/2/license.txtff6eaa8b858ea317fded99f125f5fcd0MD52TEXT1111.pdf.txt1111.pdf.txtExtracted texttext/plain51581https://repositorio.ufba.br/bitstream/ri/13738/3/1111.pdf.txtf53c0b36532bdac41a100da6bde99b8fMD53ri/137382022-08-08 13:17:42.344oai:repositorio.ufba.br:ri/13738VGVybW8gZGUgTGljZW7vv71hLCBu77+9byBleGNsdXNpdm8sIHBhcmEgbyBkZXDvv71zaXRvIG5vIFJlcG9zaXTvv71yaW8gSW5zdGl0dWNpb25hbCBkYSBVRkJBLgoKIFBlbG8gcHJvY2Vzc28gZGUgc3VibWlzc++/vW8gZGUgZG9jdW1lbnRvcywgbyBhdXRvciBvdSBzZXUgcmVwcmVzZW50YW50ZSBsZWdhbCwgYW8gYWNlaXRhciAKZXNzZSB0ZXJtbyBkZSBsaWNlbu+/vWEsIGNvbmNlZGUgYW8gUmVwb3NpdO+/vXJpbyBJbnN0aXR1Y2lvbmFsIGRhIFVuaXZlcnNpZGFkZSBGZWRlcmFsIGRhIEJhaGlhIApvIGRpcmVpdG8gZGUgbWFudGVyIHVtYSBj77+9cGlhIGVtIHNldSByZXBvc2l077+9cmlvIGNvbSBhIGZpbmFsaWRhZGUsIHByaW1laXJhLCBkZSBwcmVzZXJ2Ye+/ve+/vW8uIApFc3NlcyB0ZXJtb3MsIG7vv71vIGV4Y2x1c2l2b3MsIG1hbnTvv71tIG9zIGRpcmVpdG9zIGRlIGF1dG9yL2NvcHlyaWdodCwgbWFzIGVudGVuZGUgbyBkb2N1bWVudG8gCmNvbW8gcGFydGUgZG8gYWNlcnZvIGludGVsZWN0dWFsIGRlc3NhIFVuaXZlcnNpZGFkZS4KCiBQYXJhIG9zIGRvY3VtZW50b3MgcHVibGljYWRvcyBjb20gcmVwYXNzZSBkZSBkaXJlaXRvcyBkZSBkaXN0cmlidWnvv73vv71vLCBlc3NlIHRlcm1vIGRlIGxpY2Vu77+9YSAKZW50ZW5kZSBxdWU6CgogTWFudGVuZG8gb3MgZGlyZWl0b3MgYXV0b3JhaXMsIHJlcGFzc2Fkb3MgYSB0ZXJjZWlyb3MsIGVtIGNhc28gZGUgcHVibGljYe+/ve+/vWVzLCBvIHJlcG9zaXTvv71yaW8KcG9kZSByZXN0cmluZ2lyIG8gYWNlc3NvIGFvIHRleHRvIGludGVncmFsLCBtYXMgbGliZXJhIGFzIGluZm9ybWHvv73vv71lcyBzb2JyZSBvIGRvY3VtZW50bwooTWV0YWRhZG9zIGVzY3JpdGl2b3MpLgoKIERlc3RhIGZvcm1hLCBhdGVuZGVuZG8gYW9zIGFuc2Vpb3MgZGVzc2EgdW5pdmVyc2lkYWRlIGVtIG1hbnRlciBzdWEgcHJvZHXvv73vv71vIGNpZW5077+9ZmljYSBjb20gCmFzIHJlc3Ryae+/ve+/vWVzIGltcG9zdGFzIHBlbG9zIGVkaXRvcmVzIGRlIHBlcmnvv71kaWNvcy4KCiBQYXJhIGFzIHB1YmxpY2Hvv73vv71lcyBzZW0gaW5pY2lhdGl2YXMgcXVlIHNlZ3VlbSBhIHBvbO+/vXRpY2EgZGUgQWNlc3NvIEFiZXJ0bywgb3MgZGVw77+9c2l0b3MgCmNvbXB1bHPvv71yaW9zIG5lc3NlIHJlcG9zaXTvv71yaW8gbWFudO+/vW0gb3MgZGlyZWl0b3MgYXV0b3JhaXMsIG1hcyBtYW5077+9bSBhY2Vzc28gaXJyZXN0cml0byAKYW8gbWV0YWRhZG9zIGUgdGV4dG8gY29tcGxldG8uIEFzc2ltLCBhIGFjZWl0Ye+/ve+/vW8gZGVzc2UgdGVybW8gbu+/vW8gbmVjZXNzaXRhIGRlIGNvbnNlbnRpbWVudG8KIHBvciBwYXJ0ZSBkZSBhdXRvcmVzL2RldGVudG9yZXMgZG9zIGRpcmVpdG9zLCBwb3IgZXN0YXJlbSBlbSBpbmljaWF0aXZhcyBkZSBhY2Vzc28gYWJlcnRvLgo=Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-08-08T16:17:42Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Dual effect of Lutzomyia longipalpis saliva on Leishmania braziliensis infection is mediated by distinct saliva-induced cellular recruitment into BALB/c mice ear
dc.title.alternative.pt_BR.fl_str_mv BMC Microbiology
title Dual effect of Lutzomyia longipalpis saliva on Leishmania braziliensis infection is mediated by distinct saliva-induced cellular recruitment into BALB/c mice ear
spellingShingle Dual effect of Lutzomyia longipalpis saliva on Leishmania braziliensis infection is mediated by distinct saliva-induced cellular recruitment into BALB/c mice ear
Carregaro, Vanessa
Phlebotomines saliva
Lutzomyia longipalpis saliva
Leishmania braziliensis
Inflammatory leucocytes
Cytokines
Immunoregulation
title_short Dual effect of Lutzomyia longipalpis saliva on Leishmania braziliensis infection is mediated by distinct saliva-induced cellular recruitment into BALB/c mice ear
title_full Dual effect of Lutzomyia longipalpis saliva on Leishmania braziliensis infection is mediated by distinct saliva-induced cellular recruitment into BALB/c mice ear
title_fullStr Dual effect of Lutzomyia longipalpis saliva on Leishmania braziliensis infection is mediated by distinct saliva-induced cellular recruitment into BALB/c mice ear
title_full_unstemmed Dual effect of Lutzomyia longipalpis saliva on Leishmania braziliensis infection is mediated by distinct saliva-induced cellular recruitment into BALB/c mice ear
title_sort Dual effect of Lutzomyia longipalpis saliva on Leishmania braziliensis infection is mediated by distinct saliva-induced cellular recruitment into BALB/c mice ear
author Carregaro, Vanessa
author_facet Carregaro, Vanessa
Costa, Diego Luis
Brodskyn, Claudia Ida
Barral, Aldina Maria Prado
Barral-Netto, Manoel
Cunha, Fernando Q
Silva, João Santana
author_role author
author2 Costa, Diego Luis
Brodskyn, Claudia Ida
Barral, Aldina Maria Prado
Barral-Netto, Manoel
Cunha, Fernando Q
Silva, João Santana
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Carregaro, Vanessa
Costa, Diego Luis
Brodskyn, Claudia Ida
Barral, Aldina Maria Prado
Barral-Netto, Manoel
Cunha, Fernando Q
Silva, João Santana
Carregaro, Vanessa
Costa, Diego Luis
Brodskyn, Claudia Ida
Barral, Aldina Maria Prado
Barral-Netto, Manoel
Cunha, Fernando Q
Silva, João Santana
dc.subject.por.fl_str_mv Phlebotomines saliva
Lutzomyia longipalpis saliva
Leishmania braziliensis
Inflammatory leucocytes
Cytokines
Immunoregulation
topic Phlebotomines saliva
Lutzomyia longipalpis saliva
Leishmania braziliensis
Inflammatory leucocytes
Cytokines
Immunoregulation
description p. 1-11
publishDate 2013
dc.date.accessioned.fl_str_mv 2013-11-19T19:47:17Z
dc.date.available.fl_str_mv 2013-11-19T19:47:17Z
dc.date.issued.fl_str_mv 2013
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/13738
dc.identifier.issn.none.fl_str_mv 1471-2180
identifier_str_mv 1471-2180
url http://repositorio.ufba.br/ri/handle/ri/13738
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BMC Microbiology
publisher.none.fl_str_mv BMC Microbiology
dc.source.pt_BR.fl_str_mv http://www.biomedcentral.com/content/pdf/1471-2180-13-102.pdf
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
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collection Repositório Institucional da UFBA
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