Development of fluorogenic probe-based PCR assays for the detection and quantification of bovine piroplasmids

Detalhes bibliográficos
Autor(a) principal: Fornelio, A. Criado
Data de Publicação: 2009
Outros Autores: Buling, A., Asenzo, G., Benitez, D., Christensen, M. Florin, Oliva, A. Gonzalez, Henriques, G., Madruga, C.R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/13746
Resumo: Texto completo: acesso restrito. p. 200–206
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spelling Fornelio, A. CriadoBuling, A.Asenzo, G.Benitez, D.Christensen, M. FlorinOliva, A. GonzalezHenriques, G.Madruga, C.R.Fornelio, A. CriadoBuling, A.Asenzo, G.Benitez, D.Christensen, M. FlorinOliva, A. GonzalezHenriques, G.Madruga, C.R.2013-11-19T19:56:35Z20090304-4017http://repositorio.ufba.br/ri/handle/ri/13746v. 162, n. 3-4Texto completo: acesso restrito. p. 200–206This paper reports two new quantitative PCR (qPCR) assays, developed in an attempt to improve the detection of bovine piroplasmids. The first of these techniques is a duplex TaqMan assay for the simultaneous diagnosis of Babesia bovis and B. bigemina. This technique is ideal for use in South America where bovids harbour no theilerids. The second technique, which is suitable for the diagnosis of both babesiosis and theileriosis worldwide, involves fluorescence resonance energy transfer (FRET) probes. In FRET assays, Babesia bovis, B. divergens, Babesia sp. (B. major or B. bigemina), Theileria annae and Theileria sp. were all identifiable based on the melting temperatures of their amplified fragments. Both techniques provided linear calibration curves over the 0.1 fg/μl to 0.01 ng/μl DNA range. The assays showed good sensitivity and specificity. To assess their performance, both procedures were compared in two separate studies: the first was intended to monitor the experimental infection of calves with B. bovis and the second was a survey where 200 bovid/equine DNA samples from different countries were screened for piroplasmids. Comparative studies showed that duplex TaqMan qPCR was more sensitive than FRET qPCR in the detection of babesids.Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2013-10-07T16:38:26Z No. of bitstreams: 1 1-s2.0-S0304401709001976-main.pdf: 458382 bytes, checksum: 1c5c303a3d853f6ec55cb2e56b7e9072 (MD5)Approved for entry into archive by Rodrigo Meirelles (rodrigomei@ufba.br) on 2013-11-19T19:56:35Z (GMT) No. of bitstreams: 1 1-s2.0-S0304401709001976-main.pdf: 458382 bytes, checksum: 1c5c303a3d853f6ec55cb2e56b7e9072 (MD5)Made available in DSpace on 2013-11-19T19:56:35Z (GMT). No. of bitstreams: 1 1-s2.0-S0304401709001976-main.pdf: 458382 bytes, checksum: 1c5c303a3d853f6ec55cb2e56b7e9072 (MD5) Previous issue date: 2009http://dx.doi.org/10.1016/j.vetpar.2009.03.040reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBABabesiaTheileriaQuantitative PCRMolecular diagnosticTaqMan probesFRET probesDevelopment of fluorogenic probe-based PCR assays for the detection and quantification of bovine piroplasmidsVeterinary Parasitologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01info:eu-repo/semantics/openAccessengORIGINAL1-s2.0-S0304401709001976-main.pdf1-s2.0-S0304401709001976-main.pdfapplication/pdf458382https://repositorio.ufba.br/bitstream/ri/13746/1/1-s2.0-S0304401709001976-main.pdf1c5c303a3d853f6ec55cb2e56b7e9072MD51LICENSElicense.txtlicense.txttext/plain1365https://repositorio.ufba.br/bitstream/ri/13746/2/license.txt5371a150bdc863f78dcf39281543bd86MD52TEXT1-s2.0-S0304401709001976-main.pdf.txt1-s2.0-S0304401709001976-main.pdf.txtExtracted texttext/plain34795https://repositorio.ufba.br/bitstream/ri/13746/3/1-s2.0-S0304401709001976-main.pdf.txt02d6832d819a52c4c694c37a50ec99d8MD53ri/137462022-07-05 14:02:48.507oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:02:48Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Development of fluorogenic probe-based PCR assays for the detection and quantification of bovine piroplasmids
dc.title.alternative.pt_BR.fl_str_mv Veterinary Parasitology
title Development of fluorogenic probe-based PCR assays for the detection and quantification of bovine piroplasmids
spellingShingle Development of fluorogenic probe-based PCR assays for the detection and quantification of bovine piroplasmids
Fornelio, A. Criado
Babesia
Theileria
Quantitative PCR
Molecular diagnostic
TaqMan probes
FRET probes
title_short Development of fluorogenic probe-based PCR assays for the detection and quantification of bovine piroplasmids
title_full Development of fluorogenic probe-based PCR assays for the detection and quantification of bovine piroplasmids
title_fullStr Development of fluorogenic probe-based PCR assays for the detection and quantification of bovine piroplasmids
title_full_unstemmed Development of fluorogenic probe-based PCR assays for the detection and quantification of bovine piroplasmids
title_sort Development of fluorogenic probe-based PCR assays for the detection and quantification of bovine piroplasmids
author Fornelio, A. Criado
author_facet Fornelio, A. Criado
Buling, A.
Asenzo, G.
Benitez, D.
Christensen, M. Florin
Oliva, A. Gonzalez
Henriques, G.
Madruga, C.R.
author_role author
author2 Buling, A.
Asenzo, G.
Benitez, D.
Christensen, M. Florin
Oliva, A. Gonzalez
Henriques, G.
Madruga, C.R.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fornelio, A. Criado
Buling, A.
Asenzo, G.
Benitez, D.
Christensen, M. Florin
Oliva, A. Gonzalez
Henriques, G.
Madruga, C.R.
Fornelio, A. Criado
Buling, A.
Asenzo, G.
Benitez, D.
Christensen, M. Florin
Oliva, A. Gonzalez
Henriques, G.
Madruga, C.R.
dc.subject.por.fl_str_mv Babesia
Theileria
Quantitative PCR
Molecular diagnostic
TaqMan probes
FRET probes
topic Babesia
Theileria
Quantitative PCR
Molecular diagnostic
TaqMan probes
FRET probes
description Texto completo: acesso restrito. p. 200–206
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2013-11-19T19:56:35Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/13746
dc.identifier.issn.none.fl_str_mv 0304-4017
dc.identifier.number.pt_BR.fl_str_mv v. 162, n. 3-4
identifier_str_mv 0304-4017
v. 162, n. 3-4
url http://repositorio.ufba.br/ri/handle/ri/13746
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1016/j.vetpar.2009.03.040
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
instacron:UFBA
instname_str Universidade Federal da Bahia (UFBA)
instacron_str UFBA
institution UFBA
reponame_str Repositório Institucional da UFBA
collection Repositório Institucional da UFBA
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