id UFBA-2_e984b8d6e096ae6c0fe01fb32b866662
oai_identifier_str oai:repositorio.ufba.br:ri/14187
network_acronym_str UFBA-2
network_name_str Repositório Institucional da UFBA
repository_id_str 1932
spelling Costa, Dirceu JoaquimCarvalho, Rayssa M. de AraujoAbbehusen, Melissa Moura CostaTeixeira, Clarissa RomeroPitombo, Maiana AlbuquerqueSantos, Joelma Trigo dosNascimento, Flávia Raquel Fernandes doSilva, Lucilene AmorimSilva, Ana Lucia AbreuCruz, Maria do Socorro PiresMiranda, José CarlosFukutani, Kyoshi FerreiraOliveira, Camila Indiani deBarral, Aldina Maria PradoBarral-Netto, ManoelBrodskyn, Claudia Ida2013-12-16T14:10:09Z2013-12-16T14:10:09Z20131932-6203http://repositorio.ufba.br/ri/handle/ri/14187v. 8, n. 4p. 1-11Background: Canine Visceral Leishmaniasis (CVL) is a zoonotic disease caused by Leishmania infantum, transmitted by the bite of Lutzomyia longipalpis sand flies. Dogs are the main domestic reservoir of the parasite. The establishment of an experimental model that partially reproduces natural infection in dogs is very important to test vaccine candidates, mainly regarding those that use salivary proteins from the vector and new therapeutical approaches. Methodology/Principal Findings: In this report, we describe an experimental infection in dogs, using intradermal injection of Leishmania infantum plus salivary gland homogenate (SGH) of Lutzomyia longipalpis. Thirty-five dogs were infected with 16107 parasites combined with five pairs of Lutzomyia longipalpis salivary glands and followed for 450 days after infection and clinical, immunological and parasitological parameters were evaluated. Two hundred and ten days after infection we observed that 31,4% of dogs did not display detectable levels of anti-Leishmania antibodies but all presented different numbers of parasites in the lymph nodes. Animals with a positive xenodiagnosis had at least 3,356105 parasites in their lymph nodes. An increase of IFN-c and IL-10 levels was detected during infection. Twenty two percent of dogs developed symptoms of CVL during infection. Conclusion: The infection model described here shows some degree of similarity when compared with naturally infected dogs opening new perspectives for the study of CVL using an experimental model that employs the combination of parasites and sand fly saliva both present during natural transmission.Submitted by Santiago Fabio (fabio.ssantiago@hotmail.com) on 2013-11-26T20:07:11Z No. of bitstreams: 1 333333.pdf: 1604899 bytes, checksum: a78c2e529c64a3400dcb92cae6906a2a (MD5)Approved for entry into archive by Patricia Barroso (pbarroso@ufba.br) on 2013-12-16T14:10:09Z (GMT) No. of bitstreams: 1 333333.pdf: 1604899 bytes, checksum: a78c2e529c64a3400dcb92cae6906a2a (MD5)Made available in DSpace on 2013-12-16T14:10:09Z (GMT). No. of bitstreams: 1 333333.pdf: 1604899 bytes, checksum: a78c2e529c64a3400dcb92cae6906a2a (MD5) Previous issue date: 2013SalvadorPLoS ONEhttp://www.plosone.org/article/fetchObject.action?uri=info%3Adoi%2F10.1371%2Fjournal.pone.0060535&representation=PDFreponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBALeishmaniasis, VisceralLeishmania infantumLeishmaniasis VaccinesExperimental Infection of Dogs with Leishmania and Saliva as a Model to Study Canine Visceral LeishmaniasisPLoS ONEinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessengORIGINAL333333.pdf333333.pdfapplication/pdf1604899https://repositorio.ufba.br/bitstream/ri/14187/1/333333.pdfa78c2e529c64a3400dcb92cae6906a2aMD51LICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/14187/2/license.txtff6eaa8b858ea317fded99f125f5fcd0MD52TEXT333333.pdf.txt333333.pdf.txtExtracted texttext/plain49619https://repositorio.ufba.br/bitstream/ri/14187/3/333333.pdf.txt2864d9b1dd8999cf7b485bddd56c3c7dMD53ri/141872022-02-21 00:10:17.283oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-02-21T03:10:17Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Experimental Infection of Dogs with Leishmania and Saliva as a Model to Study Canine Visceral Leishmaniasis
dc.title.alternative.pt_BR.fl_str_mv PLoS ONE
title Experimental Infection of Dogs with Leishmania and Saliva as a Model to Study Canine Visceral Leishmaniasis
spellingShingle Experimental Infection of Dogs with Leishmania and Saliva as a Model to Study Canine Visceral Leishmaniasis
Costa, Dirceu Joaquim
Leishmaniasis, Visceral
Leishmania infantum
Leishmaniasis Vaccines
title_short Experimental Infection of Dogs with Leishmania and Saliva as a Model to Study Canine Visceral Leishmaniasis
title_full Experimental Infection of Dogs with Leishmania and Saliva as a Model to Study Canine Visceral Leishmaniasis
title_fullStr Experimental Infection of Dogs with Leishmania and Saliva as a Model to Study Canine Visceral Leishmaniasis
title_full_unstemmed Experimental Infection of Dogs with Leishmania and Saliva as a Model to Study Canine Visceral Leishmaniasis
title_sort Experimental Infection of Dogs with Leishmania and Saliva as a Model to Study Canine Visceral Leishmaniasis
author Costa, Dirceu Joaquim
author_facet Costa, Dirceu Joaquim
Carvalho, Rayssa M. de Araujo
Abbehusen, Melissa Moura Costa
Teixeira, Clarissa Romero
Pitombo, Maiana Albuquerque
Santos, Joelma Trigo dos
Nascimento, Flávia Raquel Fernandes do
Silva, Lucilene Amorim
Silva, Ana Lucia Abreu
Cruz, Maria do Socorro Pires
Miranda, José Carlos
Fukutani, Kyoshi Ferreira
Oliveira, Camila Indiani de
Barral, Aldina Maria Prado
Barral-Netto, Manoel
Brodskyn, Claudia Ida
author_role author
author2 Carvalho, Rayssa M. de Araujo
Abbehusen, Melissa Moura Costa
Teixeira, Clarissa Romero
Pitombo, Maiana Albuquerque
Santos, Joelma Trigo dos
Nascimento, Flávia Raquel Fernandes do
Silva, Lucilene Amorim
Silva, Ana Lucia Abreu
Cruz, Maria do Socorro Pires
Miranda, José Carlos
Fukutani, Kyoshi Ferreira
Oliveira, Camila Indiani de
Barral, Aldina Maria Prado
Barral-Netto, Manoel
Brodskyn, Claudia Ida
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Costa, Dirceu Joaquim
Carvalho, Rayssa M. de Araujo
Abbehusen, Melissa Moura Costa
Teixeira, Clarissa Romero
Pitombo, Maiana Albuquerque
Santos, Joelma Trigo dos
Nascimento, Flávia Raquel Fernandes do
Silva, Lucilene Amorim
Silva, Ana Lucia Abreu
Cruz, Maria do Socorro Pires
Miranda, José Carlos
Fukutani, Kyoshi Ferreira
Oliveira, Camila Indiani de
Barral, Aldina Maria Prado
Barral-Netto, Manoel
Brodskyn, Claudia Ida
dc.subject.por.fl_str_mv Leishmaniasis, Visceral
Leishmania infantum
Leishmaniasis Vaccines
topic Leishmaniasis, Visceral
Leishmania infantum
Leishmaniasis Vaccines
description p. 1-11
publishDate 2013
dc.date.accessioned.fl_str_mv 2013-12-16T14:10:09Z
dc.date.available.fl_str_mv 2013-12-16T14:10:09Z
dc.date.issued.fl_str_mv 2013
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/14187
dc.identifier.issn.none.fl_str_mv 1932-6203
dc.identifier.number.pt_BR.fl_str_mv v. 8, n. 4
identifier_str_mv 1932-6203
v. 8, n. 4
url http://repositorio.ufba.br/ri/handle/ri/14187
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv PLoS ONE
publisher.none.fl_str_mv PLoS ONE
dc.source.pt_BR.fl_str_mv http://www.plosone.org/article/fetchObject.action?uri=info%3Adoi%2F10.1371%2Fjournal.pone.0060535&representation=PDF
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
instacron:UFBA
instname_str Universidade Federal da Bahia (UFBA)
instacron_str UFBA
institution UFBA
reponame_str Repositório Institucional da UFBA
collection Repositório Institucional da UFBA
bitstream.url.fl_str_mv https://repositorio.ufba.br/bitstream/ri/14187/1/333333.pdf
https://repositorio.ufba.br/bitstream/ri/14187/2/license.txt
https://repositorio.ufba.br/bitstream/ri/14187/3/333333.pdf.txt
bitstream.checksum.fl_str_mv a78c2e529c64a3400dcb92cae6906a2a
ff6eaa8b858ea317fded99f125f5fcd0
2864d9b1dd8999cf7b485bddd56c3c7d
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)
repository.mail.fl_str_mv
_version_ 1808459465676029952