Purification of a lectin with antibacterial activity from Bothrops leucurus snake venom

Detalhes bibliográficos
Autor(a) principal: Nunes, Erika dos Santos
Data de Publicação: 2011
Outros Autores: Souza, Mary Angela Aranda de, Vaz, Antônio Fernando de Melo, Santana, Giselly Maria de Sá, Gomes, Francis Soares, Coelho, Luana Cassandra Breitenbach Barroso, Paiva, Patrícia Maria Guedes, Silva, Rejane Maria Lira da, Silva-Lucca, Rosemeire Aparecida, Oliva, Maria Luiza Vilela, Guarnieri, Miriam Camargo, Correia, Maria Tereza dos Santos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/5430
Resumo: Acesso restrito: Texto completo. p. 57-63.
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spelling Nunes, Erika dos SantosSouza, Mary Angela Aranda deVaz, Antônio Fernando de MeloSantana, Giselly Maria de SáGomes, Francis SoaresCoelho, Luana Cassandra Breitenbach BarrosoPaiva, Patrícia Maria GuedesSilva, Rejane Maria Lira daSilva-Lucca, Rosemeire AparecidaOliva, Maria Luiza VilelaGuarnieri, Miriam CamargoCorreia, Maria Tereza dos SantosNunes, Erika dos SantosSouza, Mary Angela Aranda deVaz, Antônio Fernando de MeloSantana, Giselly Maria de SáGomes, Francis SoaresCoelho, Luana Cassandra Breitenbach BarrosoPaiva, Patrícia Maria GuedesSilva, Rejane Maria Lira daSilva-Lucca, Rosemeire AparecidaOliva, Maria Luiza VilelaGuarnieri, Miriam CamargoCorreia, Maria Tereza dos Santos2012-02-24T12:27:40Z2011http://www.repositorio.ufba.br/ri/handle/ri/5430v. 159, n. 1.Acesso restrito: Texto completo. p. 57-63.A novel lectin was isolated from Bothrops leucurus snake venom using a combination of affinity and gel filtration chromatographies. The lectin (BlL) agglutinated glutaraldehyde-treated rabbit and human erythrocytes with preference for rabbit erythrocytes. Galactose, raffinose, lactose, fetal bovine serum and casein inhibited lectin-induced rabbit erythrocyte agglutination. BlL, with a molecular mass of 30 kDa and composed of two subunits of 15 kDa, showed dependence on calcium. BlL is an acidic protein with highest activity over the pH range of 4.0–7.0 and stable under heating to 70 °C. Fluorescence emission spectra showed tryptophan residues partially buried within the lectin structure. The percentages of secondary structure revealed by circular dichroism were 1% α-helix, 44% β-sheet, 24% β-turn and 31% unordered. BlL showed effective antibacterial activity against Gram-positive bacteria Staphylococcus aureus, Enterococcus faecalis and Bacillus subtilis with minimal inhibitory concentrations of 31.25, 62.25 and 125 μg/mL, respectively. In conclusion, B. leucurus snake venom contains a galactoside-binding lectin with antibacterial activity.Submitted by JURANDI DE SOUZA SILVA (jssufba@hotmail.com) on 2012-02-24T12:27:40Z No. of bitstreams: 1 __pdn.sciencedirect.com_....0-S109649591100039X-main.pdf: 285488 bytes, checksum: eb5db11aed712ad4983cbc91c31fc451 (MD5)Made available in DSpace on 2012-02-24T12:27:40Z (GMT). No. of bitstreams: 1 __pdn.sciencedirect.com_....0-S109649591100039X-main.pdf: 285488 bytes, checksum: eb5db11aed712ad4983cbc91c31fc451 (MD5) Previous issue date: 2011DOI: 10.1016/j.cbpb.2011.02.001reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAAntibacterial activityFluorescenceCircular dichroismBothrops leucurusLectinSnake venomPurification of a lectin with antibacterial activity from Bothrops leucurus snake venomComparative biochemistry and physiology b-biochemistry & molecular biologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01enginfo:eu-repo/semantics/openAccessORIGINAL__pdn.sciencedirect.com_....0-S109649591100039X-main.pdf__pdn.sciencedirect.com_....0-S109649591100039X-main.pdfapplication/pdf285488https://repositorio.ufba.br/bitstream/ri/5430/1/__pdn.sciencedirect.com_....0-S109649591100039X-main.pdfeb5db11aed712ad4983cbc91c31fc451MD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/5430/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXT__pdn.sciencedirect.com_....0-S109649591100039X-main.pdf.txt__pdn.sciencedirect.com_....0-S109649591100039X-main.pdf.txtExtracted texttext/plain44095https://repositorio.ufba.br/bitstream/ri/5430/3/__pdn.sciencedirect.com_....0-S109649591100039X-main.pdf.txt16d76980526327fb9aff9de8de374d17MD53ri/54302022-07-05 14:03:22.463oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:22Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Purification of a lectin with antibacterial activity from Bothrops leucurus snake venom
dc.title.alternative.pt_BR.fl_str_mv Comparative biochemistry and physiology b-biochemistry & molecular biology
title Purification of a lectin with antibacterial activity from Bothrops leucurus snake venom
spellingShingle Purification of a lectin with antibacterial activity from Bothrops leucurus snake venom
Nunes, Erika dos Santos
Antibacterial activity
Fluorescence
Circular dichroism
Bothrops leucurus
Lectin
Snake venom
title_short Purification of a lectin with antibacterial activity from Bothrops leucurus snake venom
title_full Purification of a lectin with antibacterial activity from Bothrops leucurus snake venom
title_fullStr Purification of a lectin with antibacterial activity from Bothrops leucurus snake venom
title_full_unstemmed Purification of a lectin with antibacterial activity from Bothrops leucurus snake venom
title_sort Purification of a lectin with antibacterial activity from Bothrops leucurus snake venom
author Nunes, Erika dos Santos
author_facet Nunes, Erika dos Santos
Souza, Mary Angela Aranda de
Vaz, Antônio Fernando de Melo
Santana, Giselly Maria de Sá
Gomes, Francis Soares
Coelho, Luana Cassandra Breitenbach Barroso
Paiva, Patrícia Maria Guedes
Silva, Rejane Maria Lira da
Silva-Lucca, Rosemeire Aparecida
Oliva, Maria Luiza Vilela
Guarnieri, Miriam Camargo
Correia, Maria Tereza dos Santos
author_role author
author2 Souza, Mary Angela Aranda de
Vaz, Antônio Fernando de Melo
Santana, Giselly Maria de Sá
Gomes, Francis Soares
Coelho, Luana Cassandra Breitenbach Barroso
Paiva, Patrícia Maria Guedes
Silva, Rejane Maria Lira da
Silva-Lucca, Rosemeire Aparecida
Oliva, Maria Luiza Vilela
Guarnieri, Miriam Camargo
Correia, Maria Tereza dos Santos
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Nunes, Erika dos Santos
Souza, Mary Angela Aranda de
Vaz, Antônio Fernando de Melo
Santana, Giselly Maria de Sá
Gomes, Francis Soares
Coelho, Luana Cassandra Breitenbach Barroso
Paiva, Patrícia Maria Guedes
Silva, Rejane Maria Lira da
Silva-Lucca, Rosemeire Aparecida
Oliva, Maria Luiza Vilela
Guarnieri, Miriam Camargo
Correia, Maria Tereza dos Santos
Nunes, Erika dos Santos
Souza, Mary Angela Aranda de
Vaz, Antônio Fernando de Melo
Santana, Giselly Maria de Sá
Gomes, Francis Soares
Coelho, Luana Cassandra Breitenbach Barroso
Paiva, Patrícia Maria Guedes
Silva, Rejane Maria Lira da
Silva-Lucca, Rosemeire Aparecida
Oliva, Maria Luiza Vilela
Guarnieri, Miriam Camargo
Correia, Maria Tereza dos Santos
dc.subject.por.fl_str_mv Antibacterial activity
Fluorescence
Circular dichroism
Bothrops leucurus
Lectin
Snake venom
topic Antibacterial activity
Fluorescence
Circular dichroism
Bothrops leucurus
Lectin
Snake venom
description Acesso restrito: Texto completo. p. 57-63.
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2012-02-24T12:27:40Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/5430
dc.identifier.number.pt_BR.fl_str_mv v. 159, n. 1.
url http://www.repositorio.ufba.br/ri/handle/ri/5430
identifier_str_mv v. 159, n. 1.
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv DOI: 10.1016/j.cbpb.2011.02.001
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
instacron:UFBA
instname_str Universidade Federal da Bahia (UFBA)
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institution UFBA
reponame_str Repositório Institucional da UFBA
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