Biocompatibility and biodegradation analysis of Nile Tilapia gelatin and apatite membranes
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista ciência agronômica (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902022000100434 |
Resumo: | ABSTRACT Fish farming by-products could represent large-scale raw materials for xenogeneic implants that could be used for bone regeneration. The objective of this research was to analyze the biocompatibility and biodegradation of gelatin (G) and apatite (A) membranes from Nile tilapia. Adult male Swiss mice underwent subcutaneous implantation of biocomposites derived from skins and scales in different concentrations: 60%G:40%A (G1), 70%G:30%A (G2) and 80%G:20%A (G3). Commercial collagen membrane (C+) or implantless surgery (C-) were used as controls. Euthanasia was performed at 1, 3 or 9 weeks for histological analysis of the inflammatory and repair criteria as well as the integrity of each material. The statistical analysis of non-parametric data was performed using the Kruskal-Wallis test and post-hoc Dunn test, with p<0.05. In vivo results during the experimental period demonstrated progressive improvement in biocompatibility, with G1 and G2 being slightly irritating and G3 non-irritating, just like C+. There were significant differences between test and control groups regarding the presence of neutrophils, lymphocytes, macrophages, foreign body giant cells, neovascularization and connective tissue. There was also a decrease in the integrity of the implants, where G1 maintained greater stability than G3 and G2, but less than C+. All biocomposites proved to be biocompatible and partially biodegradable. G1 suggests greater potential for use as an osteopromoting membrane, with its biological performance associated with higher mineral concentration compared to the organic phase. Future long-term orthotopic studies will be conducted to investigate its osteopromoting action. |
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Biocompatibility and biodegradation analysis of Nile Tilapia gelatin and apatite membranesGelatinApatitesBiocompatible materialsAbsorbable implantsABSTRACT Fish farming by-products could represent large-scale raw materials for xenogeneic implants that could be used for bone regeneration. The objective of this research was to analyze the biocompatibility and biodegradation of gelatin (G) and apatite (A) membranes from Nile tilapia. Adult male Swiss mice underwent subcutaneous implantation of biocomposites derived from skins and scales in different concentrations: 60%G:40%A (G1), 70%G:30%A (G2) and 80%G:20%A (G3). Commercial collagen membrane (C+) or implantless surgery (C-) were used as controls. Euthanasia was performed at 1, 3 or 9 weeks for histological analysis of the inflammatory and repair criteria as well as the integrity of each material. The statistical analysis of non-parametric data was performed using the Kruskal-Wallis test and post-hoc Dunn test, with p<0.05. In vivo results during the experimental period demonstrated progressive improvement in biocompatibility, with G1 and G2 being slightly irritating and G3 non-irritating, just like C+. There were significant differences between test and control groups regarding the presence of neutrophils, lymphocytes, macrophages, foreign body giant cells, neovascularization and connective tissue. There was also a decrease in the integrity of the implants, where G1 maintained greater stability than G3 and G2, but less than C+. All biocomposites proved to be biocompatible and partially biodegradable. G1 suggests greater potential for use as an osteopromoting membrane, with its biological performance associated with higher mineral concentration compared to the organic phase. Future long-term orthotopic studies will be conducted to investigate its osteopromoting action.Universidade Federal do Ceará2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902022000100434Revista Ciência Agronômica v.53 2022reponame:Revista ciência agronômica (Online)instname:Universidade Federal do Ceará (UFC)instacron:UFC10.5935/1806-6690.20220035info:eu-repo/semantics/openAccessSousa,Efigênia Maria deMelo,Edla Freire deRibeiro,Hálisson LucasFeitosa,Judith Pessoa de AndradeSouza-Filho,Men de Sá Moreira deMelo,Marcelo Miranda deCastro-Silva,Igor Iucoeng2022-05-12T00:00:00Zoai:scielo:S1806-66902022000100434Revistahttp://www.ccarevista.ufc.br/PUBhttps://old.scielo.br/oai/scielo-oai.php||alekdutra@ufc.br|| ccarev@ufc.br1806-66900045-6888opendoar:2022-05-12T00:00Revista ciência agronômica (Online) - Universidade Federal do Ceará (UFC)false |
dc.title.none.fl_str_mv |
Biocompatibility and biodegradation analysis of Nile Tilapia gelatin and apatite membranes |
title |
Biocompatibility and biodegradation analysis of Nile Tilapia gelatin and apatite membranes |
spellingShingle |
Biocompatibility and biodegradation analysis of Nile Tilapia gelatin and apatite membranes Sousa,Efigênia Maria de Gelatin Apatites Biocompatible materials Absorbable implants |
title_short |
Biocompatibility and biodegradation analysis of Nile Tilapia gelatin and apatite membranes |
title_full |
Biocompatibility and biodegradation analysis of Nile Tilapia gelatin and apatite membranes |
title_fullStr |
Biocompatibility and biodegradation analysis of Nile Tilapia gelatin and apatite membranes |
title_full_unstemmed |
Biocompatibility and biodegradation analysis of Nile Tilapia gelatin and apatite membranes |
title_sort |
Biocompatibility and biodegradation analysis of Nile Tilapia gelatin and apatite membranes |
author |
Sousa,Efigênia Maria de |
author_facet |
Sousa,Efigênia Maria de Melo,Edla Freire de Ribeiro,Hálisson Lucas Feitosa,Judith Pessoa de Andrade Souza-Filho,Men de Sá Moreira de Melo,Marcelo Miranda de Castro-Silva,Igor Iuco |
author_role |
author |
author2 |
Melo,Edla Freire de Ribeiro,Hálisson Lucas Feitosa,Judith Pessoa de Andrade Souza-Filho,Men de Sá Moreira de Melo,Marcelo Miranda de Castro-Silva,Igor Iuco |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Sousa,Efigênia Maria de Melo,Edla Freire de Ribeiro,Hálisson Lucas Feitosa,Judith Pessoa de Andrade Souza-Filho,Men de Sá Moreira de Melo,Marcelo Miranda de Castro-Silva,Igor Iuco |
dc.subject.por.fl_str_mv |
Gelatin Apatites Biocompatible materials Absorbable implants |
topic |
Gelatin Apatites Biocompatible materials Absorbable implants |
description |
ABSTRACT Fish farming by-products could represent large-scale raw materials for xenogeneic implants that could be used for bone regeneration. The objective of this research was to analyze the biocompatibility and biodegradation of gelatin (G) and apatite (A) membranes from Nile tilapia. Adult male Swiss mice underwent subcutaneous implantation of biocomposites derived from skins and scales in different concentrations: 60%G:40%A (G1), 70%G:30%A (G2) and 80%G:20%A (G3). Commercial collagen membrane (C+) or implantless surgery (C-) were used as controls. Euthanasia was performed at 1, 3 or 9 weeks for histological analysis of the inflammatory and repair criteria as well as the integrity of each material. The statistical analysis of non-parametric data was performed using the Kruskal-Wallis test and post-hoc Dunn test, with p<0.05. In vivo results during the experimental period demonstrated progressive improvement in biocompatibility, with G1 and G2 being slightly irritating and G3 non-irritating, just like C+. There were significant differences between test and control groups regarding the presence of neutrophils, lymphocytes, macrophages, foreign body giant cells, neovascularization and connective tissue. There was also a decrease in the integrity of the implants, where G1 maintained greater stability than G3 and G2, but less than C+. All biocomposites proved to be biocompatible and partially biodegradable. G1 suggests greater potential for use as an osteopromoting membrane, with its biological performance associated with higher mineral concentration compared to the organic phase. Future long-term orthotopic studies will be conducted to investigate its osteopromoting action. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902022000100434 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902022000100434 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.5935/1806-6690.20220035 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal do Ceará |
publisher.none.fl_str_mv |
Universidade Federal do Ceará |
dc.source.none.fl_str_mv |
Revista Ciência Agronômica v.53 2022 reponame:Revista ciência agronômica (Online) instname:Universidade Federal do Ceará (UFC) instacron:UFC |
instname_str |
Universidade Federal do Ceará (UFC) |
instacron_str |
UFC |
institution |
UFC |
reponame_str |
Revista ciência agronômica (Online) |
collection |
Revista ciência agronômica (Online) |
repository.name.fl_str_mv |
Revista ciência agronômica (Online) - Universidade Federal do Ceará (UFC) |
repository.mail.fl_str_mv |
||alekdutra@ufc.br|| ccarev@ufc.br |
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1750297490527092736 |