Accuracy of buffy coat in the diagnosis of disseminated histoplasmosis in AIDS-patients in an endemic area of Brazil

Detalhes bibliográficos
Autor(a) principal: Leitão, Terezinha do Menino Jesus Silva
Data de Publicação: 2019
Outros Autores: Oliveira Filho, Antonio M. P., Sousa Filho, José Evaldo P., Tavares, Bruno M., Mesquita, Jacó R. L., Farias, Luís Arthur B. G., Mota, Rosa S., Nacher, Mathieu, Damasceno, Lisandra Serra
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da Universidade Federal do Ceará (UFC)
Texto Completo: http://www.repositorio.ufc.br/handle/riufc/46535
Resumo: The bu y coat is obtained routinely for disseminated histoplamosis (DH) diagnosis in Ceará, Brazil. The aim of this study is to describe the accuracy of staining smears for Histoplasma in the bu y coat of AIDS-patients with DH. From 2012–2013, all results of stained bu y coat smears and culture for fungi performed at São José Hospital were recorded. In total, 489 bu y coats of 361 patients were studied; 19/361 (5.3%; 95%CI = 2.9–7.6%) had positive direct examination stained smears for Histoplasma and 61/361 (16.9%; 95%CI = 13.0–20.8%) had growth in culture. For those with positive Histoplasma cultures, the CD4 count was significantly lower (139.3 vs. 191.7cells/ L; p = 0.014) than others, and death was 18%. The sensitivity and specificity of stained smears was 25.9% and 100%, respectively. A second test, performed up to 36 days from the first one, increased the sensitivity of stained smears to 32.2%. Stained smears of bu y coat have low accuracy; nonetheless, they are easy to perform and can give a quick diagnosis in low-resource endemic areas. Despite the decrease in mortality, it is not yet to the low levels observed in areas that have better and more e cient methods.
id UFC-7_83539857e1aeb3cf36543e2744dbb3b9
oai_identifier_str oai:repositorio.ufc.br:riufc/46535
network_acronym_str UFC-7
network_name_str Repositório Institucional da Universidade Federal do Ceará (UFC)
repository_id_str
spelling Accuracy of buffy coat in the diagnosis of disseminated histoplasmosis in AIDS-patients in an endemic area of BrazilDiagnósticoHistoplasmoseHistoplasmosisSíndrome de Imunodeficiência AdquiridaAcquired Immunodeficiency SyndromeThe bu y coat is obtained routinely for disseminated histoplamosis (DH) diagnosis in Ceará, Brazil. The aim of this study is to describe the accuracy of staining smears for Histoplasma in the bu y coat of AIDS-patients with DH. From 2012–2013, all results of stained bu y coat smears and culture for fungi performed at São José Hospital were recorded. In total, 489 bu y coats of 361 patients were studied; 19/361 (5.3%; 95%CI = 2.9–7.6%) had positive direct examination stained smears for Histoplasma and 61/361 (16.9%; 95%CI = 13.0–20.8%) had growth in culture. For those with positive Histoplasma cultures, the CD4 count was significantly lower (139.3 vs. 191.7cells/ L; p = 0.014) than others, and death was 18%. The sensitivity and specificity of stained smears was 25.9% and 100%, respectively. A second test, performed up to 36 days from the first one, increased the sensitivity of stained smears to 32.2%. Stained smears of bu y coat have low accuracy; nonetheless, they are easy to perform and can give a quick diagnosis in low-resource endemic areas. Despite the decrease in mortality, it is not yet to the low levels observed in areas that have better and more e cient methods.Journal of Fungi2019-10-07T13:56:32Z2019-10-07T13:56:32Z2019-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfLEITÃO, Terezinha M. J. Silva et al. Accuracy of buffy coat in the diagnosis of disseminated histoplasmosis in AIDS-patients in an endemic area of Brazil. Journal of Fungi, v. 5, n. 2, p. 1-8, jun. 2019.2309-608Xhttp://www.repositorio.ufc.br/handle/riufc/46535Leitão, Terezinha do Menino Jesus SilvaOliveira Filho, Antonio M. P.Sousa Filho, José Evaldo P.Tavares, Bruno M.Mesquita, Jacó R. L.Farias, Luís Arthur B. G.Mota, Rosa S.Nacher, MathieuDamasceno, Lisandra Serraengreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccess2022-12-16T16:53:45Zoai:repositorio.ufc.br:riufc/46535Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2024-09-11T18:58:58.996639Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false
dc.title.none.fl_str_mv Accuracy of buffy coat in the diagnosis of disseminated histoplasmosis in AIDS-patients in an endemic area of Brazil
title Accuracy of buffy coat in the diagnosis of disseminated histoplasmosis in AIDS-patients in an endemic area of Brazil
spellingShingle Accuracy of buffy coat in the diagnosis of disseminated histoplasmosis in AIDS-patients in an endemic area of Brazil
Leitão, Terezinha do Menino Jesus Silva
Diagnóstico
Histoplasmose
Histoplasmosis
Síndrome de Imunodeficiência Adquirida
Acquired Immunodeficiency Syndrome
title_short Accuracy of buffy coat in the diagnosis of disseminated histoplasmosis in AIDS-patients in an endemic area of Brazil
title_full Accuracy of buffy coat in the diagnosis of disseminated histoplasmosis in AIDS-patients in an endemic area of Brazil
title_fullStr Accuracy of buffy coat in the diagnosis of disseminated histoplasmosis in AIDS-patients in an endemic area of Brazil
title_full_unstemmed Accuracy of buffy coat in the diagnosis of disseminated histoplasmosis in AIDS-patients in an endemic area of Brazil
title_sort Accuracy of buffy coat in the diagnosis of disseminated histoplasmosis in AIDS-patients in an endemic area of Brazil
author Leitão, Terezinha do Menino Jesus Silva
author_facet Leitão, Terezinha do Menino Jesus Silva
Oliveira Filho, Antonio M. P.
Sousa Filho, José Evaldo P.
Tavares, Bruno M.
Mesquita, Jacó R. L.
Farias, Luís Arthur B. G.
Mota, Rosa S.
Nacher, Mathieu
Damasceno, Lisandra Serra
author_role author
author2 Oliveira Filho, Antonio M. P.
Sousa Filho, José Evaldo P.
Tavares, Bruno M.
Mesquita, Jacó R. L.
Farias, Luís Arthur B. G.
Mota, Rosa S.
Nacher, Mathieu
Damasceno, Lisandra Serra
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Leitão, Terezinha do Menino Jesus Silva
Oliveira Filho, Antonio M. P.
Sousa Filho, José Evaldo P.
Tavares, Bruno M.
Mesquita, Jacó R. L.
Farias, Luís Arthur B. G.
Mota, Rosa S.
Nacher, Mathieu
Damasceno, Lisandra Serra
dc.subject.por.fl_str_mv Diagnóstico
Histoplasmose
Histoplasmosis
Síndrome de Imunodeficiência Adquirida
Acquired Immunodeficiency Syndrome
topic Diagnóstico
Histoplasmose
Histoplasmosis
Síndrome de Imunodeficiência Adquirida
Acquired Immunodeficiency Syndrome
description The bu y coat is obtained routinely for disseminated histoplamosis (DH) diagnosis in Ceará, Brazil. The aim of this study is to describe the accuracy of staining smears for Histoplasma in the bu y coat of AIDS-patients with DH. From 2012–2013, all results of stained bu y coat smears and culture for fungi performed at São José Hospital were recorded. In total, 489 bu y coats of 361 patients were studied; 19/361 (5.3%; 95%CI = 2.9–7.6%) had positive direct examination stained smears for Histoplasma and 61/361 (16.9%; 95%CI = 13.0–20.8%) had growth in culture. For those with positive Histoplasma cultures, the CD4 count was significantly lower (139.3 vs. 191.7cells/ L; p = 0.014) than others, and death was 18%. The sensitivity and specificity of stained smears was 25.9% and 100%, respectively. A second test, performed up to 36 days from the first one, increased the sensitivity of stained smears to 32.2%. Stained smears of bu y coat have low accuracy; nonetheless, they are easy to perform and can give a quick diagnosis in low-resource endemic areas. Despite the decrease in mortality, it is not yet to the low levels observed in areas that have better and more e cient methods.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-07T13:56:32Z
2019-10-07T13:56:32Z
2019-06
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv LEITÃO, Terezinha M. J. Silva et al. Accuracy of buffy coat in the diagnosis of disseminated histoplasmosis in AIDS-patients in an endemic area of Brazil. Journal of Fungi, v. 5, n. 2, p. 1-8, jun. 2019.
2309-608X
http://www.repositorio.ufc.br/handle/riufc/46535
identifier_str_mv LEITÃO, Terezinha M. J. Silva et al. Accuracy of buffy coat in the diagnosis of disseminated histoplasmosis in AIDS-patients in an endemic area of Brazil. Journal of Fungi, v. 5, n. 2, p. 1-8, jun. 2019.
2309-608X
url http://www.repositorio.ufc.br/handle/riufc/46535
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Journal of Fungi
publisher.none.fl_str_mv Journal of Fungi
dc.source.none.fl_str_mv reponame:Repositório Institucional da Universidade Federal do Ceará (UFC)
instname:Universidade Federal do Ceará (UFC)
instacron:UFC
instname_str Universidade Federal do Ceará (UFC)
instacron_str UFC
institution UFC
reponame_str Repositório Institucional da Universidade Federal do Ceará (UFC)
collection Repositório Institucional da Universidade Federal do Ceará (UFC)
repository.name.fl_str_mv Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)
repository.mail.fl_str_mv bu@ufc.br || repositorio@ufc.br
_version_ 1813029021799153664