Análise tensional, morfológica e morfométrica da anastomose cólica na colite induzida por ácido acético a 10%, em ratos wistar, tratados com extrato aquoso de aroeira-do-sertão a 10% (myracrodruon urundeuva fr. all.)

Detalhes bibliográficos
Autor(a) principal: Cavalcante, Antônio Rubens Soares Martins
Data de Publicação: 2004
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da Universidade Federal do Ceará (UFC)
Texto Completo: http://www.repositorio.ufc.br/handle/riufc/7287
Resumo: The colorectal anastomosis awakens interest for the studies because it presents elevated morbidity and mortality. This occurs mainly, by dehiscence, attributed to several factors, including inflammatory. The aroeira-do-sertão (Myracrodruon urundeuva Fr. All.), a plant of the Anacardiaceae family, is known for its antiinflammatory and healing properties, appearing as a alternative therapy. Facing these known qualities, it was decided to verify its action on the colonic anastomosis while under 10% acetic acid induced experimental colitis, in Wistar rats. Were utilized 48 rats,males, with average weight 340g, distributed in two groups of 24. One experiment group formed by rats that were treated with 10% aqueous extract of aroeira-do-sertão and one control group treated with carboxymethylcellulose (CMC) based vehicle, both in the enema form. Each group was divided into four subgroups according to the date of euthanasia (days 3, 7, 14 and 21). Twenty-four hours after the inducion of colitis the animals were subjected to endoscopic examination and after that laparotomy for the performance of the total section of the colon and posterior colonic anastomosis, end-to-end, in single plane with 5-0 polypropylene. The animals were evaluated from the morphological (disruption of epithelial basement membranecontinuity, fibrin clot, edema, exudate, vascularity, connective new formation and reepithelization), morphometric (neutrophils, eosinophils, histiocytes, lymphocyte, plasma cells, fibroblasts and vessels) and rupture pression (explosion) of the colonic segment point of view. On the histological study dyed by trichrome stain it was evaluated the collagen proliferation. The colonic segments were removed for studies on the respective dates of euthanasia. On the morphological analysis it was observed a superiority on Aroeira group over Vehicle group on the twenty-first day. On the morphometric evaluation, it was noticed no antiinflammatory action on the part of aroeira, but it presented results superior to the control group on the second and third phases of healing that correspond respectively to the phase of fibroplasia (fibroblastic proliferation) and maturation and remodeling which is collagen deposition on the healing matrix. The pressoric level was statistically significant on the third day, being the Aroeira Group superior to the Vehicle Group. It is concluded that the 10% aqueous extract of aroeira-do-sertão, develops healing activity on the colonic anastomosis under 10% acetic acid induced experimental colitis, in Wistar rats, acting on phase two and three of healing increasing the fibroplasias and the collagen deposition on the healing matrix.
id UFC-7_862f9ba8363aa1a69a470bc39d4a27c1
oai_identifier_str oai:repositorio.ufc.br:riufc/7287
network_acronym_str UFC-7
network_name_str Repositório Institucional da Universidade Federal do Ceará (UFC)
repository_id_str
spelling Análise tensional, morfológica e morfométrica da anastomose cólica na colite induzida por ácido acético a 10%, em ratos wistar, tratados com extrato aquoso de aroeira-do-sertão a 10% (myracrodruon urundeuva fr. all.)Tensional, morphological and morphometric analysis of the colonic anastomosis under 10% acetic acid induced colitis, in Wistar rats, treated with 10% aqueous extract of aroeira-do-sertão (Myracrodruon urundeuva Fr. All.)Cirurgia ColorretalColitePlantas MedicinaisThe colorectal anastomosis awakens interest for the studies because it presents elevated morbidity and mortality. This occurs mainly, by dehiscence, attributed to several factors, including inflammatory. The aroeira-do-sertão (Myracrodruon urundeuva Fr. All.), a plant of the Anacardiaceae family, is known for its antiinflammatory and healing properties, appearing as a alternative therapy. Facing these known qualities, it was decided to verify its action on the colonic anastomosis while under 10% acetic acid induced experimental colitis, in Wistar rats. Were utilized 48 rats,males, with average weight 340g, distributed in two groups of 24. One experiment group formed by rats that were treated with 10% aqueous extract of aroeira-do-sertão and one control group treated with carboxymethylcellulose (CMC) based vehicle, both in the enema form. Each group was divided into four subgroups according to the date of euthanasia (days 3, 7, 14 and 21). Twenty-four hours after the inducion of colitis the animals were subjected to endoscopic examination and after that laparotomy for the performance of the total section of the colon and posterior colonic anastomosis, end-to-end, in single plane with 5-0 polypropylene. The animals were evaluated from the morphological (disruption of epithelial basement membranecontinuity, fibrin clot, edema, exudate, vascularity, connective new formation and reepithelization), morphometric (neutrophils, eosinophils, histiocytes, lymphocyte, plasma cells, fibroblasts and vessels) and rupture pression (explosion) of the colonic segment point of view. On the histological study dyed by trichrome stain it was evaluated the collagen proliferation. The colonic segments were removed for studies on the respective dates of euthanasia. On the morphological analysis it was observed a superiority on Aroeira group over Vehicle group on the twenty-first day. On the morphometric evaluation, it was noticed no antiinflammatory action on the part of aroeira, but it presented results superior to the control group on the second and third phases of healing that correspond respectively to the phase of fibroplasia (fibroblastic proliferation) and maturation and remodeling which is collagen deposition on the healing matrix. The pressoric level was statistically significant on the third day, being the Aroeira Group superior to the Vehicle Group. It is concluded that the 10% aqueous extract of aroeira-do-sertão, develops healing activity on the colonic anastomosis under 10% acetic acid induced experimental colitis, in Wistar rats, acting on phase two and three of healing increasing the fibroplasias and the collagen deposition on the healing matrix.O estudo anastomose colorretal desperta interesse por apresentar morbidade e mortalidade elevadas. Isso ocorre principalmente, por deiscência, atribuída a vários fatores, inclusive inflamatórios. A aroeira-do-sertão (Myracrodruon urundeuva Fr. All.), árvore da família Anacardiaceae, é conhecida pelas suas propriedades antiinflamatórias e cicatrizantes, surgindo como uma alternativa terapêutica. Diante dessas qualidades conhecidas, decidiu-se verificar sua ação na anastomose cólica na vigência da colite experimental induzida por ácido acético a 10%, em ratos Wistar. Foram utilizados 48 ratos, machos, com peso médio de 340g, distribuídos em dois grupos de 24. Um grupo experimento formado por ratos que foram tratados com extrato aquoso de aroeira a 10% e um grupo controle tratado com o veículo à base de carboximetilcelulose (CMC), ambos sob forma de enema. Cada grupo foi dividido em quatro subgrupos conforme a data da eutanásia (3, 7, 14 e 21 dias). Vinte e quatro horas após a indução da colite os animais foram submetidos ao exame endoscópico e em seguida à laparotomia para a realização da secção total do cólon e posterior anastomose cólica, término-terminal, em plano único com fio de polipropileno 5-0. Os animais foram avaliados do ponto de vista morfológico (solução de continuidade, crosta fibrino-leucocitária, edema, exsudato, neoformação vascular, neoformação conjuntiva e reepitelização), morfométrico (neutrófilos, eosinófilos, histiócitos, linfócitos, plasmócitos, fibroblastos e vasos) e pressão de ruptura (explosão) do segmento colônico. No estudo histológico corado pelo Tricrômico de Masson avaliou-se a proliferação do colágeno. Os segmentos colônicos foram retirados para estudos nas respectivas datas da eutanásia. Na análise morfológica observou-se uma superioridade do grupo Aroeira sobre o grupo Veículo no vigésimo primeiro dia. Na avaliação morfométrica, não ficou evidenciado nenhuma ação antiinflamatória por parte da aroeira, porém ela apresentou resultados superiores ao grupo controle na segunda e terceira fase da cicatrização que correspondem respectivamente a fase de fibroplasia (proliferação fibroblástica) e maturação e remodelagem que é a deposição de colágeno na matriz cicatricial. O nível pressórico foi estatisticamente significante no terceiro dia, sendo o Grupo Aroeira superior ao Grupo Veículo. Conclui-se que o extrato aquoso de aroeira-do-sertão a 10%, desenvolve atividade cicatrizante na anastomose cólica na vigência de colite experimental induzida por ácido acético a 10%, em ratos Wistar, atuando na fase dois e três da cicatrização aumentando a fibroplasia e a deposição de colágeno na matriz cicatricial.Rodrigues , Lusmar VerasCavalcante, Antônio Rubens Soares Martins2014-02-18T11:03:07Z2014-02-18T11:03:07Z2004info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfCAVALCANTE, Antonio Rubens Soares Martins. Análise tensional, morfológica e morfométrica da anastomose cólica na colite induzida por ácido acético a 10% (myracrodruon urundeuva fr. all.). 2004. 102 f. Dissertação (Mestrado em Cirurgia) - Universidade Federal do Ceará. Faculdade de Medicina, Fortaleza, 2004.http://www.repositorio.ufc.br/handle/riufc/7287porreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccess2018-12-13T11:24:25Zoai:repositorio.ufc.br:riufc/7287Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2024-09-11T18:41:51.044229Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false
dc.title.none.fl_str_mv Análise tensional, morfológica e morfométrica da anastomose cólica na colite induzida por ácido acético a 10%, em ratos wistar, tratados com extrato aquoso de aroeira-do-sertão a 10% (myracrodruon urundeuva fr. all.)
Tensional, morphological and morphometric analysis of the colonic anastomosis under 10% acetic acid induced colitis, in Wistar rats, treated with 10% aqueous extract of aroeira-do-sertão (Myracrodruon urundeuva Fr. All.)
title Análise tensional, morfológica e morfométrica da anastomose cólica na colite induzida por ácido acético a 10%, em ratos wistar, tratados com extrato aquoso de aroeira-do-sertão a 10% (myracrodruon urundeuva fr. all.)
spellingShingle Análise tensional, morfológica e morfométrica da anastomose cólica na colite induzida por ácido acético a 10%, em ratos wistar, tratados com extrato aquoso de aroeira-do-sertão a 10% (myracrodruon urundeuva fr. all.)
Cavalcante, Antônio Rubens Soares Martins
Cirurgia Colorretal
Colite
Plantas Medicinais
title_short Análise tensional, morfológica e morfométrica da anastomose cólica na colite induzida por ácido acético a 10%, em ratos wistar, tratados com extrato aquoso de aroeira-do-sertão a 10% (myracrodruon urundeuva fr. all.)
title_full Análise tensional, morfológica e morfométrica da anastomose cólica na colite induzida por ácido acético a 10%, em ratos wistar, tratados com extrato aquoso de aroeira-do-sertão a 10% (myracrodruon urundeuva fr. all.)
title_fullStr Análise tensional, morfológica e morfométrica da anastomose cólica na colite induzida por ácido acético a 10%, em ratos wistar, tratados com extrato aquoso de aroeira-do-sertão a 10% (myracrodruon urundeuva fr. all.)
title_full_unstemmed Análise tensional, morfológica e morfométrica da anastomose cólica na colite induzida por ácido acético a 10%, em ratos wistar, tratados com extrato aquoso de aroeira-do-sertão a 10% (myracrodruon urundeuva fr. all.)
title_sort Análise tensional, morfológica e morfométrica da anastomose cólica na colite induzida por ácido acético a 10%, em ratos wistar, tratados com extrato aquoso de aroeira-do-sertão a 10% (myracrodruon urundeuva fr. all.)
author Cavalcante, Antônio Rubens Soares Martins
author_facet Cavalcante, Antônio Rubens Soares Martins
author_role author
dc.contributor.none.fl_str_mv Rodrigues , Lusmar Veras
dc.contributor.author.fl_str_mv Cavalcante, Antônio Rubens Soares Martins
dc.subject.por.fl_str_mv Cirurgia Colorretal
Colite
Plantas Medicinais
topic Cirurgia Colorretal
Colite
Plantas Medicinais
description The colorectal anastomosis awakens interest for the studies because it presents elevated morbidity and mortality. This occurs mainly, by dehiscence, attributed to several factors, including inflammatory. The aroeira-do-sertão (Myracrodruon urundeuva Fr. All.), a plant of the Anacardiaceae family, is known for its antiinflammatory and healing properties, appearing as a alternative therapy. Facing these known qualities, it was decided to verify its action on the colonic anastomosis while under 10% acetic acid induced experimental colitis, in Wistar rats. Were utilized 48 rats,males, with average weight 340g, distributed in two groups of 24. One experiment group formed by rats that were treated with 10% aqueous extract of aroeira-do-sertão and one control group treated with carboxymethylcellulose (CMC) based vehicle, both in the enema form. Each group was divided into four subgroups according to the date of euthanasia (days 3, 7, 14 and 21). Twenty-four hours after the inducion of colitis the animals were subjected to endoscopic examination and after that laparotomy for the performance of the total section of the colon and posterior colonic anastomosis, end-to-end, in single plane with 5-0 polypropylene. The animals were evaluated from the morphological (disruption of epithelial basement membranecontinuity, fibrin clot, edema, exudate, vascularity, connective new formation and reepithelization), morphometric (neutrophils, eosinophils, histiocytes, lymphocyte, plasma cells, fibroblasts and vessels) and rupture pression (explosion) of the colonic segment point of view. On the histological study dyed by trichrome stain it was evaluated the collagen proliferation. The colonic segments were removed for studies on the respective dates of euthanasia. On the morphological analysis it was observed a superiority on Aroeira group over Vehicle group on the twenty-first day. On the morphometric evaluation, it was noticed no antiinflammatory action on the part of aroeira, but it presented results superior to the control group on the second and third phases of healing that correspond respectively to the phase of fibroplasia (fibroblastic proliferation) and maturation and remodeling which is collagen deposition on the healing matrix. The pressoric level was statistically significant on the third day, being the Aroeira Group superior to the Vehicle Group. It is concluded that the 10% aqueous extract of aroeira-do-sertão, develops healing activity on the colonic anastomosis under 10% acetic acid induced experimental colitis, in Wistar rats, acting on phase two and three of healing increasing the fibroplasias and the collagen deposition on the healing matrix.
publishDate 2004
dc.date.none.fl_str_mv 2004
2014-02-18T11:03:07Z
2014-02-18T11:03:07Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv CAVALCANTE, Antonio Rubens Soares Martins. Análise tensional, morfológica e morfométrica da anastomose cólica na colite induzida por ácido acético a 10% (myracrodruon urundeuva fr. all.). 2004. 102 f. Dissertação (Mestrado em Cirurgia) - Universidade Federal do Ceará. Faculdade de Medicina, Fortaleza, 2004.
http://www.repositorio.ufc.br/handle/riufc/7287
identifier_str_mv CAVALCANTE, Antonio Rubens Soares Martins. Análise tensional, morfológica e morfométrica da anastomose cólica na colite induzida por ácido acético a 10% (myracrodruon urundeuva fr. all.). 2004. 102 f. Dissertação (Mestrado em Cirurgia) - Universidade Federal do Ceará. Faculdade de Medicina, Fortaleza, 2004.
url http://www.repositorio.ufc.br/handle/riufc/7287
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Institucional da Universidade Federal do Ceará (UFC)
instname:Universidade Federal do Ceará (UFC)
instacron:UFC
instname_str Universidade Federal do Ceará (UFC)
instacron_str UFC
institution UFC
reponame_str Repositório Institucional da Universidade Federal do Ceará (UFC)
collection Repositório Institucional da Universidade Federal do Ceará (UFC)
repository.name.fl_str_mv Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)
repository.mail.fl_str_mv bu@ufc.br || repositorio@ufc.br
_version_ 1813028910421508096