Try574 leu mutation confers cross-resistance to ALS-inhibiting herbicides in wild radish
Autor(a) principal: | |
---|---|
Data de Publicação: | 2024 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Caatinga |
Texto Completo: | https://periodicos.ufersa.edu.br/caatinga/article/view/11974 |
Resumo: | Understanding how weeds resist herbicides, their resistance mechanisms, and alternative control methods are crucial for managing herbicide-resistant weeds. This study aims to unravel the resistance mechanism of a Raphanus raphanistrum biotype to acetolactate synthase (ALS) inhibitors. To this end, dose-response studies, DNA sequencing, and metabolic pathway verification were conducted. ALS-inhibiting herbicides showed low efficacy in controlling this biotype, confirming cross-resistance. Sequencing of the ALS enzyme revealed the presence of the previously reported Try-574-Leu mutation, known to confer cross-resistance to this mode of action. However, the metabolization verification assay demonstrated that this mechanism did not contribute to the observed resistance. Chemical control studies with alternative herbicides yielded promising results, indicating the potential for effective management of the resistant biotype. Our findings showed that the wild radish biotype used exhibits cross-resistance to ALS-inhibiting herbicides due to the presence of the Try-574-Leu mutation in the target enzyme. Notably, herbicides with alternative mechanisms of action prove highly effective in controlling this resistant biotype, offering valuable options for weed management strategies. |
id |
UFERSA-1_e417fddfd7e85ad7e219c344db90b226 |
---|---|
oai_identifier_str |
oai:ojs.periodicos.ufersa.edu.br:article/11974 |
network_acronym_str |
UFERSA-1 |
network_name_str |
Revista Caatinga |
repository_id_str |
|
spelling |
Try574 leu mutation confers cross-resistance to ALS-inhibiting herbicides in wild radishMutação Try574 conferindo resistência cruzada a herbicidas inibidores da ALS em nabiçaPolimorfismo de nucleotídio simples. Raphanus raphanistrum. Trigo. Controle de plantas daninhas.Single-nucleotide polymorphism. Raphanus raphanistrum. Wheat. Weed Control.Understanding how weeds resist herbicides, their resistance mechanisms, and alternative control methods are crucial for managing herbicide-resistant weeds. This study aims to unravel the resistance mechanism of a Raphanus raphanistrum biotype to acetolactate synthase (ALS) inhibitors. To this end, dose-response studies, DNA sequencing, and metabolic pathway verification were conducted. ALS-inhibiting herbicides showed low efficacy in controlling this biotype, confirming cross-resistance. Sequencing of the ALS enzyme revealed the presence of the previously reported Try-574-Leu mutation, known to confer cross-resistance to this mode of action. However, the metabolization verification assay demonstrated that this mechanism did not contribute to the observed resistance. Chemical control studies with alternative herbicides yielded promising results, indicating the potential for effective management of the resistant biotype. Our findings showed that the wild radish biotype used exhibits cross-resistance to ALS-inhibiting herbicides due to the presence of the Try-574-Leu mutation in the target enzyme. Notably, herbicides with alternative mechanisms of action prove highly effective in controlling this resistant biotype, offering valuable options for weed management strategies.A caracterização da resistência de plantas daninhas a herbicidas, bem como os estudos sobre os mecanismos de resistência e as alternativas para o controle químico são fundamentais para o manejo de plantas daninhas resistentes a herbicidas. Desta forma, este trabalho teve como objetivo reportar e elucidar o mecanismo de resistência de um biótipo de Raphanus raphanistrum a inibidores da acetolactato-sintase (ALS). Foram desenvolvidos trabalhos de dose resposta, sequenciamento do gene ALS, verificação de metabolização e alternativas para o controle químico. Ocorreu baixa eficiência de controle de herbicidas inibidores de ALS a esse biótipo, sendo confirmada a resistência cruzada. O sequenciamento gene ALS demostrou que o biótipo apresentou a mutação Try-574-Leu, já reportada na literatura e que confere resistência cruzada a este mecanismo de ação. O ensaio para verificação de metabolização provou que esse mecanismo não está envolvido na resistência. Já o estudo de controle químico indicou que existem alternativas para o controle do biótipo resistente. Conclui-se que o biótipo de nabiça apresentou resistência cruzada aos herbicidas inibidores de ALS, com a mutação Try-574-Leu na enzima alvo e que herbicidas de outros mecanismos de ação promovem elevada eficiência de controle.Universidade Federal Rural do Semi-Árido2024-02-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufersa.edu.br/caatinga/article/view/1197410.1590/1983-21252024v3711974rcREVISTA CAATINGA; Vol. 37 (2024); e11974Revista Caatinga; v. 37 (2024); e119741983-21250100-316Xreponame:Revista Caatingainstname:Universidade Federal Rural do Semi-Árido (UFERSA)instacron:UFERSAenghttps://periodicos.ufersa.edu.br/caatinga/article/view/11974/11457Copyright (c) 2024 Revista Caatingainfo:eu-repo/semantics/openAccessFruet, Diogo LuizSchelter, Mayra LuizaPereira, Fernando SartoriGuerra, NaiaraSilva, Fabio Nascimento daOliveira Neto, Antonio Mendes de2024-04-22T17:59:19Zoai:ojs.periodicos.ufersa.edu.br:article/11974Revistahttps://periodicos.ufersa.edu.br/index.php/caatinga/indexPUBhttps://periodicos.ufersa.edu.br/index.php/caatinga/oaipatricio@ufersa.edu.br|| caatinga@ufersa.edu.br1983-21250100-316Xopendoar:2024-04-29T09:47:06.457351Revista Caatinga - Universidade Federal Rural do Semi-Árido (UFERSA)true |
dc.title.none.fl_str_mv |
Try574 leu mutation confers cross-resistance to ALS-inhibiting herbicides in wild radish Mutação Try574 conferindo resistência cruzada a herbicidas inibidores da ALS em nabiça |
title |
Try574 leu mutation confers cross-resistance to ALS-inhibiting herbicides in wild radish |
spellingShingle |
Try574 leu mutation confers cross-resistance to ALS-inhibiting herbicides in wild radish Fruet, Diogo Luiz Polimorfismo de nucleotídio simples. Raphanus raphanistrum. Trigo. Controle de plantas daninhas. Single-nucleotide polymorphism. Raphanus raphanistrum. Wheat. Weed Control. |
title_short |
Try574 leu mutation confers cross-resistance to ALS-inhibiting herbicides in wild radish |
title_full |
Try574 leu mutation confers cross-resistance to ALS-inhibiting herbicides in wild radish |
title_fullStr |
Try574 leu mutation confers cross-resistance to ALS-inhibiting herbicides in wild radish |
title_full_unstemmed |
Try574 leu mutation confers cross-resistance to ALS-inhibiting herbicides in wild radish |
title_sort |
Try574 leu mutation confers cross-resistance to ALS-inhibiting herbicides in wild radish |
author |
Fruet, Diogo Luiz |
author_facet |
Fruet, Diogo Luiz Schelter, Mayra Luiza Pereira, Fernando Sartori Guerra, Naiara Silva, Fabio Nascimento da Oliveira Neto, Antonio Mendes de |
author_role |
author |
author2 |
Schelter, Mayra Luiza Pereira, Fernando Sartori Guerra, Naiara Silva, Fabio Nascimento da Oliveira Neto, Antonio Mendes de |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Fruet, Diogo Luiz Schelter, Mayra Luiza Pereira, Fernando Sartori Guerra, Naiara Silva, Fabio Nascimento da Oliveira Neto, Antonio Mendes de |
dc.subject.por.fl_str_mv |
Polimorfismo de nucleotídio simples. Raphanus raphanistrum. Trigo. Controle de plantas daninhas. Single-nucleotide polymorphism. Raphanus raphanistrum. Wheat. Weed Control. |
topic |
Polimorfismo de nucleotídio simples. Raphanus raphanistrum. Trigo. Controle de plantas daninhas. Single-nucleotide polymorphism. Raphanus raphanistrum. Wheat. Weed Control. |
description |
Understanding how weeds resist herbicides, their resistance mechanisms, and alternative control methods are crucial for managing herbicide-resistant weeds. This study aims to unravel the resistance mechanism of a Raphanus raphanistrum biotype to acetolactate synthase (ALS) inhibitors. To this end, dose-response studies, DNA sequencing, and metabolic pathway verification were conducted. ALS-inhibiting herbicides showed low efficacy in controlling this biotype, confirming cross-resistance. Sequencing of the ALS enzyme revealed the presence of the previously reported Try-574-Leu mutation, known to confer cross-resistance to this mode of action. However, the metabolization verification assay demonstrated that this mechanism did not contribute to the observed resistance. Chemical control studies with alternative herbicides yielded promising results, indicating the potential for effective management of the resistant biotype. Our findings showed that the wild radish biotype used exhibits cross-resistance to ALS-inhibiting herbicides due to the presence of the Try-574-Leu mutation in the target enzyme. Notably, herbicides with alternative mechanisms of action prove highly effective in controlling this resistant biotype, offering valuable options for weed management strategies. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024-02-06 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.ufersa.edu.br/caatinga/article/view/11974 10.1590/1983-21252024v3711974rc |
url |
https://periodicos.ufersa.edu.br/caatinga/article/view/11974 |
identifier_str_mv |
10.1590/1983-21252024v3711974rc |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://periodicos.ufersa.edu.br/caatinga/article/view/11974/11457 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2024 Revista Caatinga info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2024 Revista Caatinga |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal Rural do Semi-Árido |
publisher.none.fl_str_mv |
Universidade Federal Rural do Semi-Árido |
dc.source.none.fl_str_mv |
REVISTA CAATINGA; Vol. 37 (2024); e11974 Revista Caatinga; v. 37 (2024); e11974 1983-2125 0100-316X reponame:Revista Caatinga instname:Universidade Federal Rural do Semi-Árido (UFERSA) instacron:UFERSA |
instname_str |
Universidade Federal Rural do Semi-Árido (UFERSA) |
instacron_str |
UFERSA |
institution |
UFERSA |
reponame_str |
Revista Caatinga |
collection |
Revista Caatinga |
repository.name.fl_str_mv |
Revista Caatinga - Universidade Federal Rural do Semi-Árido (UFERSA) |
repository.mail.fl_str_mv |
patricio@ufersa.edu.br|| caatinga@ufersa.edu.br |
_version_ |
1797674030301446144 |