Microbiota aeróbia do sêmen e da mucosa prepucial e efeito dos antimicrobianos na conservação dos parâmetros espermáticos de catetos (pecari tajacu linnaeus, 1758) em cativeiro
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) |
Texto Completo: | https://repositorio.ufersa.edu.br/handle/prefix/6972 |
Resumo: | The presence of bacteria in the semen can cause losses in sperm function and, consequently, interfere with reproductive biotechnologies that make use of this biological material. That said, the objective was to describe the aerobic bacterial microbiota of captive collared peccaries (Pecari tajacu) semen and foreskin, as well as the impact of the use of antimicrobial substances on the bacterial load and sperm function during the semen conservation of this species. In the first experiment, aerobic bacteria were cultured in semen and foreskin samples from nine animals, as well as their quantification and evaluation of sperm parameters in the semen. The isolates were identified and tested against concentrations of penicillin-streptomycin, gentamicin and Aloe vera gel. Corynebacterium spp. and Staphylococcus spp. were isolated in greater numbers in semen (64.1% and 20.5%, respectively) and in the foreskin (60.6% and 24.2%, respectively), ranging from 0.4 to 21 × 105 colony-forming units (CFU) per milliliter. The load of Corynebacterium spp. it was negatively correlated (P <0.05) with the integrity of the sperm membrane (r = −0.73055) and curvilinear velocity (r = −0.69048). The combination of streptomycin-penicillin (SP) and gentamicin (G) inhibited the majority of microorganisms and A. vera demonstrated a weak antimicrobial potential. In the second experiment, the toxicity of antimicrobials on sperm longevity was analyzed by the thermoresistance test in six semen samples, each diluted in Tris alone (control) and Tris plus the streptomycin-penicillin combination (2 mg/mL - 2000 IU/mL) or gentamicin (70 μg/mL), and maintained for 180 min at 37 °C. It was found that the treatments did not differ (P > 0.05) until 180 min, but that the treatment containing G reduced (P < 0.05) the sperm membrane integrity and mitochondrial activity at 180 min (53, 1 ± 7.1% and 50.7 ± 6.2%, respectively) if compared to 0 min (80.5 ± 4.7% and 86.3 ± 3.4%, respectively). In the third experiment, the effect of two concentrations of SP (2 mg/mL - 2000 IU/mL [2] and 1 mg/mL - 1000 IU/mL [1]) and G (70 μg/mL [7] and 30 μg/mL [3]) added to Tris + 20% egg yolk (TE) and Tris + 20% A. vera (TA) diluents on bacterial load and sperm quality in 10 semen samples kept under refrigeration (5 ° C) up to 36 h. The treatment containing SP2, SP1 and G7, regardless of the diluent, controlled (P < 0.05) the bacterial growth during storage (ranging from 0.5 ± 0.3 103 to 10 ± 4.1 x 103 CFU/mL) up to 36 h. Treatments diluted with TE with any of the antimicrobials showed no differences (P > 0.05) for bacterial load and sperm parameters between 0 and 36 h. TA treatments, with or without antimicrobials, affected (P < 0.05) the sperm membrane integrity and mitochondrial activity after 12 h. The TE-G7 treatment stood out for maintaining some sperm variables for a longer time, such as total motility (41.9 ± 6.1%) and progressive (15 ± 2.6%) up to 24 h, as well as membrane integrity (58.3 ± 2.1%) and curvilinear velocity (76.7 ± 5.8%) up to 36 h. Given the results, it was demonstrated the occurrence of bacterial contaminants in semen and foreskin peccaries bred in captivity, especially Corynebacterium spp. and Staphylococcus spp., as well as a negative impact of the first on sperm function in fresh semen. The streptomycin-penicillin combination (2 mg/mL - 2000 IU/mL) and gentamicin (70 μg/mL) can be added to Tris in the semen dilution, incubated at 37 °C for up to 120 min, without causing toxic effects to sperm. These drugs were also effective in controlling the bacteria present in the refrigerated semen of these animals, for up to 36 h, without affecting sperm longevity |
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Microbiota aeróbia do sêmen e da mucosa prepucial e efeito dos antimicrobianos na conservação dos parâmetros espermáticos de catetos (pecari tajacu linnaeus, 1758) em cativeiroAloe veraBactériasRefrigeraçãoTermo-resistênciaAloe veraBacteriaRefrigerationThermoresistanceCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIAThe presence of bacteria in the semen can cause losses in sperm function and, consequently, interfere with reproductive biotechnologies that make use of this biological material. That said, the objective was to describe the aerobic bacterial microbiota of captive collared peccaries (Pecari tajacu) semen and foreskin, as well as the impact of the use of antimicrobial substances on the bacterial load and sperm function during the semen conservation of this species. In the first experiment, aerobic bacteria were cultured in semen and foreskin samples from nine animals, as well as their quantification and evaluation of sperm parameters in the semen. The isolates were identified and tested against concentrations of penicillin-streptomycin, gentamicin and Aloe vera gel. Corynebacterium spp. and Staphylococcus spp. were isolated in greater numbers in semen (64.1% and 20.5%, respectively) and in the foreskin (60.6% and 24.2%, respectively), ranging from 0.4 to 21 × 105 colony-forming units (CFU) per milliliter. The load of Corynebacterium spp. it was negatively correlated (P <0.05) with the integrity of the sperm membrane (r = −0.73055) and curvilinear velocity (r = −0.69048). The combination of streptomycin-penicillin (SP) and gentamicin (G) inhibited the majority of microorganisms and A. vera demonstrated a weak antimicrobial potential. In the second experiment, the toxicity of antimicrobials on sperm longevity was analyzed by the thermoresistance test in six semen samples, each diluted in Tris alone (control) and Tris plus the streptomycin-penicillin combination (2 mg/mL - 2000 IU/mL) or gentamicin (70 μg/mL), and maintained for 180 min at 37 °C. It was found that the treatments did not differ (P > 0.05) until 180 min, but that the treatment containing G reduced (P < 0.05) the sperm membrane integrity and mitochondrial activity at 180 min (53, 1 ± 7.1% and 50.7 ± 6.2%, respectively) if compared to 0 min (80.5 ± 4.7% and 86.3 ± 3.4%, respectively). In the third experiment, the effect of two concentrations of SP (2 mg/mL - 2000 IU/mL [2] and 1 mg/mL - 1000 IU/mL [1]) and G (70 μg/mL [7] and 30 μg/mL [3]) added to Tris + 20% egg yolk (TE) and Tris + 20% A. vera (TA) diluents on bacterial load and sperm quality in 10 semen samples kept under refrigeration (5 ° C) up to 36 h. The treatment containing SP2, SP1 and G7, regardless of the diluent, controlled (P < 0.05) the bacterial growth during storage (ranging from 0.5 ± 0.3 103 to 10 ± 4.1 x 103 CFU/mL) up to 36 h. Treatments diluted with TE with any of the antimicrobials showed no differences (P > 0.05) for bacterial load and sperm parameters between 0 and 36 h. TA treatments, with or without antimicrobials, affected (P < 0.05) the sperm membrane integrity and mitochondrial activity after 12 h. The TE-G7 treatment stood out for maintaining some sperm variables for a longer time, such as total motility (41.9 ± 6.1%) and progressive (15 ± 2.6%) up to 24 h, as well as membrane integrity (58.3 ± 2.1%) and curvilinear velocity (76.7 ± 5.8%) up to 36 h. Given the results, it was demonstrated the occurrence of bacterial contaminants in semen and foreskin peccaries bred in captivity, especially Corynebacterium spp. and Staphylococcus spp., as well as a negative impact of the first on sperm function in fresh semen. The streptomycin-penicillin combination (2 mg/mL - 2000 IU/mL) and gentamicin (70 μg/mL) can be added to Tris in the semen dilution, incubated at 37 °C for up to 120 min, without causing toxic effects to sperm. These drugs were also effective in controlling the bacteria present in the refrigerated semen of these animals, for up to 36 h, without affecting sperm longevityA presença de bactérias no sêmen pode causar perdas na função espermática e, consequentemente, interferir nas biotecnologias reprodutivas que façam uso desse material biológico. Dito isso, objetivou-se descrever a microbiota bacteriana aeróbia do sêmen e prepúcio dos catetos (Pecari tajacu) criados em cativeiro, bem como o impacto do uso de substâncias antimicrobianas na carga bacteriana e função espermática durante a conservação do sêmen dessa espécie. No primeiro experimento, foi realizada a cultura das bactérias aeróbias em amostras de sêmen e prepúcio de nove animais, bem como a quantificação destas e avaliação de parâmetros espermáticos no sêmen. Os isolados foram identificados e testados frente a concentrações de penicilina-estreptomicina, gentamicina e do gel da Aloe vera. Corynebacterium spp. e Staphylococcus spp. foram isolados em maior número no sêmen (64,1% e 20,5%, respectivamente) e no prepúcio (60,6% e 24,2%, respectivamente), variando de 0,4 a 21 × 105 unidades formadoras de colônia (UFC) por mililitro. A carga de Corynebacterium spp. foi correlacionada negativamente (P < 0,05) com a integridade da membrana espermática (r = −0.73055) e velocidade curvilinear (r = −0.69048). A combinação penicilina-estreptomicina (PE) e gentamicina (G) inibiram a maioria dos microrganismos enquanto a A. vera demonstrou baixa atividade antimicrobiana. No segundo experimento, foi analisada a toxicidade de antimicrobianos sobre a longevidade espermática pelo teste de termo resistência em seis amostras de sêmen, sendo cada uma diluída em Tris (controle) e em Tris acrescido da combinação penicilina-estreptomicina (2000 UI/mL-2 mg/mL) ou de gentamicina (70 μg/mL), e mantidas por 180 min a 37 °C. Verificou-se que os tratamentos não diferiram (P > 0,05) até 180 min, mas que o tratamento contendo G reduziu (P < 0,05) a integridade da membrana e a atividade mitocondrial das células espermáticas aos 180 min (53,1 ± 7,1% e 50,7 ± 6,2%, respectivamente) se comparado a 0 min (80,5 ± 4,7% e 86,3 ± 3,4%, respectivamente). No terceiro experimento, foi avaliado o efeito de duas concentrações de PE (2000 UI/mL – 2 mg/mL [2] e 1000 UI/mL – 1 mg/mL [1]) e G (70 μg/mL [7] e 30 μg/mL [3]) adicionadas aos diluentes Tris + 20% de gema de ovo (TG) e Tris + 20% de A. vera (TA) sobre a carga bacteriana e qualidade espermática em 10 amostras de sêmen mantidas sob refrigeração (5 °C) até 36 h. O tratamento contendo PE2, PE1 e G7, independente do diluente, controlaram (P < 0,05) o crescimento bacteriano durante o armazenamento (variando de 0.5 ± 0.3 103 a 10 ± 4.1 x 103 UFC/mL) até 36 h. Os tratamentos diluídos com TG com qualquer um dos antimicrobianos não demonstraram diferenças (P > 0,05) para a carga bacteriana e parâmetros espermáticos entre 0 e 36 h. Os tratamentos com TA, com ou sem antimicrobianos, afetaram (P < 0,05) a integridade da membrana e atividade mitocondrial dos espermatozoides após 12 h. O tratamento TG-G7 se destacou por manter algumas variáveis espermáticas por mais tempo, como a motilidade total (41,9 ± 6,1%) e progressiva (15 ± 2,6%) até 24 h, bem como a integridade de membrana (58.3 ± 2.1%) e velocidade curvilínea (76.7 ± 5.8%) até 36 h. Diante dos resultados, demonstrou-se a ocorrência de bactérias contaminantes no sêmen e prepúcio de catetos criados em cativeiro, com destaque para Corynebacterium spp. e Staphylococcus spp., bem como um impacto negativo da primeira sobre a função espermática no sêmen fresco. A combinação penicilina-estreptomicina (2000 UI/mL-2 mg/mL) e gentamicina (70 μg/mL) podem ser adicionadas ao Tris na diluição de sêmen, incubado a 37 °C por até 120 min, sem causar efeitos tóxicos aos espermatozoides. Estas drogas também foram eficazes no controle das bactérias presentes no sêmen refrigerado destes animais, por até 36 h, sem causar prejuizo a longevidade espermáticaTrabalho não financiado por agência de fomento, ou autofinanciadoUniversidade Federal Rural do Semi-ÁridoBrasilCentro de Ciências Agrárias - CCAUFERSAPrograma de Pós-Graduação em Ciência AnimalFeijó, Francisco Marlon Carneiro41688597387http://lattes.cnpq.br/7436750766676260Silva, Alexandre Rodrigues70298254387http://lattes.cnpq.br/1959482950237684Pereira, Alexsandra Fernandes91307198368http://lattes.cnpq.br/8114638410593492Aires, Caio Augusto Martins94768242200http://lattes.cnpq.br/2927115194581869Mota, Rinaldo Aparecido59653981668http://lattes.cnpq.br/8378380401850485Santos, Caio Sérgio2022-05-04T19:50:18Z2020-02-212022-05-04T19:50:18Z2020-02-18info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfSantos (2020) (SANTOS, 2020)https://repositorio.ufersa.edu.br/handle/prefix/6972porSANTOS, Caio Sérgio. Microbiota aeróbia do sêmen e da mucosa prepucial e efeito dos antimicrobianos na conservação dos parâmetros espermáticos de catetos (pecari tajacu linnaeus, 1758) em cativeiro. 2020. 137 f. Tese (Doutorado em Ciência Animal), Universidade Federal Rural do Semi-Árido, Mossoró, 2020.CC-BY-SAhttps://creativecommons.org/licenses/by-sa/4.0/deed.pt_BRinfo:eu-repo/semantics/openAccessreponame:Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU)instname:Universidade Federal Rural do Semi-Árido (UFERSA)instacron:UFERSA2023-10-30T20:27:43Zoai:repositorio.ufersa.edu.br:prefix/6972Repositório Institucionalhttps://repositorio.ufersa.edu.br/PUBhttps://repositorio.ufersa.edu.br/server/oai/requestrepositorio@ufersa.edu.br || admrepositorio@ufersa.edu.bropendoar:2023-10-30T20:27:43Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) - Universidade Federal Rural do Semi-Árido (UFERSA)false |
dc.title.none.fl_str_mv |
Microbiota aeróbia do sêmen e da mucosa prepucial e efeito dos antimicrobianos na conservação dos parâmetros espermáticos de catetos (pecari tajacu linnaeus, 1758) em cativeiro |
title |
Microbiota aeróbia do sêmen e da mucosa prepucial e efeito dos antimicrobianos na conservação dos parâmetros espermáticos de catetos (pecari tajacu linnaeus, 1758) em cativeiro |
spellingShingle |
Microbiota aeróbia do sêmen e da mucosa prepucial e efeito dos antimicrobianos na conservação dos parâmetros espermáticos de catetos (pecari tajacu linnaeus, 1758) em cativeiro Santos, Caio Sérgio Aloe vera Bactérias Refrigeração Termo-resistência Aloe vera Bacteria Refrigeration Thermoresistance CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Microbiota aeróbia do sêmen e da mucosa prepucial e efeito dos antimicrobianos na conservação dos parâmetros espermáticos de catetos (pecari tajacu linnaeus, 1758) em cativeiro |
title_full |
Microbiota aeróbia do sêmen e da mucosa prepucial e efeito dos antimicrobianos na conservação dos parâmetros espermáticos de catetos (pecari tajacu linnaeus, 1758) em cativeiro |
title_fullStr |
Microbiota aeróbia do sêmen e da mucosa prepucial e efeito dos antimicrobianos na conservação dos parâmetros espermáticos de catetos (pecari tajacu linnaeus, 1758) em cativeiro |
title_full_unstemmed |
Microbiota aeróbia do sêmen e da mucosa prepucial e efeito dos antimicrobianos na conservação dos parâmetros espermáticos de catetos (pecari tajacu linnaeus, 1758) em cativeiro |
title_sort |
Microbiota aeróbia do sêmen e da mucosa prepucial e efeito dos antimicrobianos na conservação dos parâmetros espermáticos de catetos (pecari tajacu linnaeus, 1758) em cativeiro |
author |
Santos, Caio Sérgio |
author_facet |
Santos, Caio Sérgio |
author_role |
author |
dc.contributor.none.fl_str_mv |
Feijó, Francisco Marlon Carneiro 41688597387 http://lattes.cnpq.br/7436750766676260 Silva, Alexandre Rodrigues 70298254387 http://lattes.cnpq.br/1959482950237684 Pereira, Alexsandra Fernandes 91307198368 http://lattes.cnpq.br/8114638410593492 Aires, Caio Augusto Martins 94768242200 http://lattes.cnpq.br/2927115194581869 Mota, Rinaldo Aparecido 59653981668 http://lattes.cnpq.br/8378380401850485 |
dc.contributor.author.fl_str_mv |
Santos, Caio Sérgio |
dc.subject.por.fl_str_mv |
Aloe vera Bactérias Refrigeração Termo-resistência Aloe vera Bacteria Refrigeration Thermoresistance CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
topic |
Aloe vera Bactérias Refrigeração Termo-resistência Aloe vera Bacteria Refrigeration Thermoresistance CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
The presence of bacteria in the semen can cause losses in sperm function and, consequently, interfere with reproductive biotechnologies that make use of this biological material. That said, the objective was to describe the aerobic bacterial microbiota of captive collared peccaries (Pecari tajacu) semen and foreskin, as well as the impact of the use of antimicrobial substances on the bacterial load and sperm function during the semen conservation of this species. In the first experiment, aerobic bacteria were cultured in semen and foreskin samples from nine animals, as well as their quantification and evaluation of sperm parameters in the semen. The isolates were identified and tested against concentrations of penicillin-streptomycin, gentamicin and Aloe vera gel. Corynebacterium spp. and Staphylococcus spp. were isolated in greater numbers in semen (64.1% and 20.5%, respectively) and in the foreskin (60.6% and 24.2%, respectively), ranging from 0.4 to 21 × 105 colony-forming units (CFU) per milliliter. The load of Corynebacterium spp. it was negatively correlated (P <0.05) with the integrity of the sperm membrane (r = −0.73055) and curvilinear velocity (r = −0.69048). The combination of streptomycin-penicillin (SP) and gentamicin (G) inhibited the majority of microorganisms and A. vera demonstrated a weak antimicrobial potential. In the second experiment, the toxicity of antimicrobials on sperm longevity was analyzed by the thermoresistance test in six semen samples, each diluted in Tris alone (control) and Tris plus the streptomycin-penicillin combination (2 mg/mL - 2000 IU/mL) or gentamicin (70 μg/mL), and maintained for 180 min at 37 °C. It was found that the treatments did not differ (P > 0.05) until 180 min, but that the treatment containing G reduced (P < 0.05) the sperm membrane integrity and mitochondrial activity at 180 min (53, 1 ± 7.1% and 50.7 ± 6.2%, respectively) if compared to 0 min (80.5 ± 4.7% and 86.3 ± 3.4%, respectively). In the third experiment, the effect of two concentrations of SP (2 mg/mL - 2000 IU/mL [2] and 1 mg/mL - 1000 IU/mL [1]) and G (70 μg/mL [7] and 30 μg/mL [3]) added to Tris + 20% egg yolk (TE) and Tris + 20% A. vera (TA) diluents on bacterial load and sperm quality in 10 semen samples kept under refrigeration (5 ° C) up to 36 h. The treatment containing SP2, SP1 and G7, regardless of the diluent, controlled (P < 0.05) the bacterial growth during storage (ranging from 0.5 ± 0.3 103 to 10 ± 4.1 x 103 CFU/mL) up to 36 h. Treatments diluted with TE with any of the antimicrobials showed no differences (P > 0.05) for bacterial load and sperm parameters between 0 and 36 h. TA treatments, with or without antimicrobials, affected (P < 0.05) the sperm membrane integrity and mitochondrial activity after 12 h. The TE-G7 treatment stood out for maintaining some sperm variables for a longer time, such as total motility (41.9 ± 6.1%) and progressive (15 ± 2.6%) up to 24 h, as well as membrane integrity (58.3 ± 2.1%) and curvilinear velocity (76.7 ± 5.8%) up to 36 h. Given the results, it was demonstrated the occurrence of bacterial contaminants in semen and foreskin peccaries bred in captivity, especially Corynebacterium spp. and Staphylococcus spp., as well as a negative impact of the first on sperm function in fresh semen. The streptomycin-penicillin combination (2 mg/mL - 2000 IU/mL) and gentamicin (70 μg/mL) can be added to Tris in the semen dilution, incubated at 37 °C for up to 120 min, without causing toxic effects to sperm. These drugs were also effective in controlling the bacteria present in the refrigerated semen of these animals, for up to 36 h, without affecting sperm longevity |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-02-21 2020-02-18 2022-05-04T19:50:18Z 2022-05-04T19:50:18Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Santos (2020) (SANTOS, 2020) https://repositorio.ufersa.edu.br/handle/prefix/6972 |
identifier_str_mv |
Santos (2020) (SANTOS, 2020) |
url |
https://repositorio.ufersa.edu.br/handle/prefix/6972 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
SANTOS, Caio Sérgio. Microbiota aeróbia do sêmen e da mucosa prepucial e efeito dos antimicrobianos na conservação dos parâmetros espermáticos de catetos (pecari tajacu linnaeus, 1758) em cativeiro. 2020. 137 f. Tese (Doutorado em Ciência Animal), Universidade Federal Rural do Semi-Árido, Mossoró, 2020. |
dc.rights.driver.fl_str_mv |
CC-BY-SA https://creativecommons.org/licenses/by-sa/4.0/deed.pt_BR info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
CC-BY-SA https://creativecommons.org/licenses/by-sa/4.0/deed.pt_BR |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal Rural do Semi-Árido Brasil Centro de Ciências Agrárias - CCA UFERSA Programa de Pós-Graduação em Ciência Animal |
publisher.none.fl_str_mv |
Universidade Federal Rural do Semi-Árido Brasil Centro de Ciências Agrárias - CCA UFERSA Programa de Pós-Graduação em Ciência Animal |
dc.source.none.fl_str_mv |
reponame:Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) instname:Universidade Federal Rural do Semi-Árido (UFERSA) instacron:UFERSA |
instname_str |
Universidade Federal Rural do Semi-Árido (UFERSA) |
instacron_str |
UFERSA |
institution |
UFERSA |
reponame_str |
Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) |
collection |
Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) |
repository.name.fl_str_mv |
Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) - Universidade Federal Rural do Semi-Árido (UFERSA) |
repository.mail.fl_str_mv |
repositorio@ufersa.edu.br || admrepositorio@ufersa.edu.br |
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1809747463437811712 |