Caracterização da resistência a anti-helmínticos e análise molecular de populações de Haemonchus contortus frente aos benzimidazóis no município de Mossoró RN
Autor(a) principal: | |
---|---|
Data de Publicação: | 2014 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFERSA |
Texto Completo: | https://repositorio.ufersa.edu.br/handle/tede/310 |
Resumo: | The increase in anthelmintic resistance in gastrointestinal nematodes on small ruminants became a worldwide issue on the development of livestock. In order to assess the resistance scenario in that context in the Rio Grande do Norte region, in vivo and in vitro resistance tests were carried out in 30 rural properties using chemicals in the avermectin, salicylanides, benzimidazoles and imidatiazols groups. For the in vivo test, group I was used as control and four other experimental groups received oral medication, being group II given albendazole (10 mg/kg), group III levamisole (7.5 mg/kg), group IV closantel (5.0 mg/kg) and group V ivermectin (0.2 mg/kg). The nematode infection was determined using fecal egg count (FEC) essays and the efficiency of each treatment was analyzed by the fecal egg count reduction test (FECRT). For the in vivo e in vitro tests, using tiabendazole, the necessary concentration to inhibit 50% of egg hatching (EC50) and assays with a 50% inhibition of larval development (LD50) were assessed, however 50% of larval development and migration inhibition assays (LD50) essays were carried out using ivermectin. For the molecular analysis Haemonchus contortus/property were isolated for the calculation of the frequency of resistant and sensitive alleles for single nucleotide polymorphisms (SNPs): F220Y, F167Y and E198A. The results obtained from in vivo tests showed that resistance was detected for all of the drugs, with leavamisole and closantel appearing as the most effective treatments with FECRT results of 84.3% and 74.6%, respectively. In the in vitro assays, tiabendazole had a result in the hatching test of EC50 0.598 µg/ml (95% IC: 0.323-0.818), while exhibiting in the larval development test a LD50 and LD99 0.518 µg/ml(95% IC: 0.432-0,887) and 4.359 µg/ml (95% IC: 3.047-8.178), respectively. For ivermectin in the larval development test, DL50 0.637 µg/ml(95% IC: 0.382 0.903 µg/ml) e DL99 19.56 µg/ml(95% IC: 11.44 46.55 µg/ml) was observed, while in the migration essay the LD50 value was 16.337 µM (95% IC: 12.7-21.3 µM). In the molecular analysis a higher frequency of the resistant allele for SNP F200Y was observed, presenting higher percentage values of 85.81% and 73.11%, while the F167Y resistant alleles had lower values ranging from 2.93% to 43.73%. In the E198A SNP, only sensitive alleles were detected. Assessing the scenario of anthelmintic resistance in the region, one notices that it s established for the main existing drugs on the market |
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Vieira, Luiz da Silvahttp://lattes.cnpq.br/5556932562390291Zaros, Lilian Giottohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4733944A8Cavalcante, Antônio Cézar Rochahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4708332J6Monteiro, Jomar Patríciohttp://lattes.cnpq.br/2104425926511321http://lattes.cnpq.br/7123984123781406Bezerra, Ana Carla Diógenes Suassuna2016-08-15T20:27:55Z2015-03-062014-11-14BEZERRA, Ana Carla Diógenes Suassuna. Anthelmintic resistance characterization and molecular analysis of Haemonchus contortus populations upon benzimidazoles in the city of Mossoró RN. 2014. 92 f. Tese (Doutorado em Sanidade e Produção Animal) - Universidade Federal Rural do Semi-Árido, Mossoró, 2014.https://repositorio.ufersa.edu.br/handle/tede/310The increase in anthelmintic resistance in gastrointestinal nematodes on small ruminants became a worldwide issue on the development of livestock. In order to assess the resistance scenario in that context in the Rio Grande do Norte region, in vivo and in vitro resistance tests were carried out in 30 rural properties using chemicals in the avermectin, salicylanides, benzimidazoles and imidatiazols groups. For the in vivo test, group I was used as control and four other experimental groups received oral medication, being group II given albendazole (10 mg/kg), group III levamisole (7.5 mg/kg), group IV closantel (5.0 mg/kg) and group V ivermectin (0.2 mg/kg). The nematode infection was determined using fecal egg count (FEC) essays and the efficiency of each treatment was analyzed by the fecal egg count reduction test (FECRT). For the in vivo e in vitro tests, using tiabendazole, the necessary concentration to inhibit 50% of egg hatching (EC50) and assays with a 50% inhibition of larval development (LD50) were assessed, however 50% of larval development and migration inhibition assays (LD50) essays were carried out using ivermectin. For the molecular analysis Haemonchus contortus/property were isolated for the calculation of the frequency of resistant and sensitive alleles for single nucleotide polymorphisms (SNPs): F220Y, F167Y and E198A. The results obtained from in vivo tests showed that resistance was detected for all of the drugs, with leavamisole and closantel appearing as the most effective treatments with FECRT results of 84.3% and 74.6%, respectively. In the in vitro assays, tiabendazole had a result in the hatching test of EC50 0.598 µg/ml (95% IC: 0.323-0.818), while exhibiting in the larval development test a LD50 and LD99 0.518 µg/ml(95% IC: 0.432-0,887) and 4.359 µg/ml (95% IC: 3.047-8.178), respectively. For ivermectin in the larval development test, DL50 0.637 µg/ml(95% IC: 0.382 0.903 µg/ml) e DL99 19.56 µg/ml(95% IC: 11.44 46.55 µg/ml) was observed, while in the migration essay the LD50 value was 16.337 µM (95% IC: 12.7-21.3 µM). In the molecular analysis a higher frequency of the resistant allele for SNP F200Y was observed, presenting higher percentage values of 85.81% and 73.11%, while the F167Y resistant alleles had lower values ranging from 2.93% to 43.73%. In the E198A SNP, only sensitive alleles were detected. Assessing the scenario of anthelmintic resistance in the region, one notices that it s established for the main existing drugs on the marketO aumento na resistência anti-helmíntica em nematóides gastrintestinais de pequenos ruminantes tornou-se um problema global para o desenvolvimento da pecuária. Nesse contexto, a fim de avaliar o cenário da resistência na região do Rio Grande do Norte foram realizados em 30 propriedades rurais testes de resistência in vivo e in vitro utilizando produtos químicos dos grupos das avermectina, salicilanilidas, benzimidazóis e imidatiazóis. Para o teste in vivo utilizou-se o grupo I controle quatro grupos experimentais, sendo grupo II albendazol (10 mg/kg), grupo III levamisol (7,5 mg/kg), grupo IV closantel (5,0 mg/kg) e grupo V ivermectina (0,2 mg/kg). A infecção por nematóides foi determinada utilizando ensaios de ovos por grama de fezes (OPG) e a eficiência de cada tratamento pelo teste de redução da contagem de ovos nas fezes (FECRT). Para os teste in vitro, foi utilizando tiabendazol, com concentrações necessária para inibir 50% de eclosão dos ovos (EC50) e ensaios com inibição de 50% do desenvolvimento larval, porém com a ivermectina foram realizados ensaios de inibição de 50% do desenvolvimento e migração larval (DL50). Para análise molecular foram isolados Haemonchus contortus em cada propriedade avaliada para cálculo da frequência de alelos resistentes e sensíveis para os polimorfismos de nucleotídeo único (SNPs): F200Y, F167Y e E198A. Os resultados obtidos com os testes in vivo e in vitro mostraram que a resistência foi detectado em todas as drogas testadas, com o levamisol e closantel apresentando-se como os tratamentos mais eficientes, com resultados FECRT de 84,3% e 74,6%, respectivamente. Nas análises in vitro o resultado do tiabendazol no teste de eclosão foi EC50 0,598 µg/ml (IC95%: 0,323-0,818), enquanto que no desenvolvimento larval uma DL50 e DL99 de 0,518 µg/ml(IC95%: 0,432-0,887) e 4,359 µg/ml(IC95%: 3,047-8,178), respectivamente. Para ivermectina no teste de desenvolvimento larval foi observado DL50 0,637 µg/ml(CI95%: 0,382 0,903 µg/ml) e DL99 19,56 µg/ml(CI95%: 11,44 46,55 µg/ml), enquanto que no de migração o valor da DL50 foi 16,337 µM (CI95%: 12,7-21,3 µM). Na análise molecular observou-se uma frequência maior do alelo resistente para o SNP F200Y apresentando porcentagens mais altas com valores de 85,81% e 73,11%, enquanto que o F167Y apresentou valores menores de alelos resistentes variando entre 43,73% e 2,93%. No SNP E198A foram detectados alelos apenas sensíveis. Avaliando o cenário da resistência anti-helmíntica na região, percebe-se que está estabelecida para as principais drogas existentes no mercadoapplication/pdfhttp://repositorio.ufersa.edu.br/retrieve/698/AnaCDSB_TESE.pdf.jpgporUniversidade Federal Rural do Semi-ÁridoPrograma de Pós-Graduação em Ciência AnimalUFERSABRCentro de Ciências Agrárias - CCACC-BY-SAinfo:eu-repo/semantics/openAccessKeywords: Small ruminants, gastrointestinal parasites, polymorphismPequenos ruminantes, parasitos gastrintestinais, polimorfismoCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIACaracterização da resistência a anti-helmínticos e análise molecular de populações de Haemonchus contortus frente aos benzimidazóis no município de Mossoró RNAnthelmintic resistance characterization and molecular analysis of Haemonchus contortus populations upon benzimidazoles in the city of Mossoró RNinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisreponame:Biblioteca Digital de Teses e Dissertações da UFERSAinstname:Universidade Federal Rural do Semi-Árido (UFERSA)instacron:UFERSATEXTAnaCDSB_TESE.pdf.txtAnaCDSB_TESE.pdf.txtExtracted texttext/plain163426https://repositorio.ufersa.edu.br//bitstream/tede/310/3/AnaCDSB_TESE.pdf.txt42e875b2f574c8b957848581f13a01ecMD53ORIGINALAnaCDSB_TESE.pdfapplication/pdf11862899https://repositorio.ufersa.edu.br//bitstream/tede/310/1/AnaCDSB_TESE.pdfdb329299185e2c022af1f916b746aae8MD51THUMBNAILAnaCDSB_TESE.pdf.jpgAnaCDSB_TESE.pdf.jpgGenerated Thumbnailimage/jpeg3832https://repositorio.ufersa.edu.br//bitstream/tede/310/2/AnaCDSB_TESE.pdf.jpgb4f9687adb374db7acc2522c9969a333MD52tede/3102022-07-05 14:05:28.74oai:repositorio.ufersa.edu.br:tede/310Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufersa.edu.br/PUBhttp://bdtd.ufersa.edu.br/oai/requestdirecaosisbi@ufersa.edu.br|| direcaosisbi@ufersa.edu.bropendoar:2022-07-05T17:05:28Biblioteca Digital de Teses e Dissertações da UFERSA - Universidade Federal Rural do Semi-Árido (UFERSA)false |
dc.title.por.fl_str_mv |
Caracterização da resistência a anti-helmínticos e análise molecular de populações de Haemonchus contortus frente aos benzimidazóis no município de Mossoró RN |
dc.title.alternative.eng.fl_str_mv |
Anthelmintic resistance characterization and molecular analysis of Haemonchus contortus populations upon benzimidazoles in the city of Mossoró RN |
title |
Caracterização da resistência a anti-helmínticos e análise molecular de populações de Haemonchus contortus frente aos benzimidazóis no município de Mossoró RN |
spellingShingle |
Caracterização da resistência a anti-helmínticos e análise molecular de populações de Haemonchus contortus frente aos benzimidazóis no município de Mossoró RN Bezerra, Ana Carla Diógenes Suassuna Keywords: Small ruminants, gastrointestinal parasites, polymorphism Pequenos ruminantes, parasitos gastrintestinais, polimorfismo CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Caracterização da resistência a anti-helmínticos e análise molecular de populações de Haemonchus contortus frente aos benzimidazóis no município de Mossoró RN |
title_full |
Caracterização da resistência a anti-helmínticos e análise molecular de populações de Haemonchus contortus frente aos benzimidazóis no município de Mossoró RN |
title_fullStr |
Caracterização da resistência a anti-helmínticos e análise molecular de populações de Haemonchus contortus frente aos benzimidazóis no município de Mossoró RN |
title_full_unstemmed |
Caracterização da resistência a anti-helmínticos e análise molecular de populações de Haemonchus contortus frente aos benzimidazóis no município de Mossoró RN |
title_sort |
Caracterização da resistência a anti-helmínticos e análise molecular de populações de Haemonchus contortus frente aos benzimidazóis no município de Mossoró RN |
author |
Bezerra, Ana Carla Diógenes Suassuna |
author_facet |
Bezerra, Ana Carla Diógenes Suassuna |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Vieira, Luiz da Silva |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/5556932562390291 |
dc.contributor.advisor-co1.fl_str_mv |
Zaros, Lilian Giotto |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4733944A8 |
dc.contributor.referee1.fl_str_mv |
Cavalcante, Antônio Cézar Rocha |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4708332J6 |
dc.contributor.referee2.fl_str_mv |
Monteiro, Jomar Patrício |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/2104425926511321 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7123984123781406 |
dc.contributor.author.fl_str_mv |
Bezerra, Ana Carla Diógenes Suassuna |
contributor_str_mv |
Vieira, Luiz da Silva Zaros, Lilian Giotto Cavalcante, Antônio Cézar Rocha Monteiro, Jomar Patrício |
dc.subject.eng.fl_str_mv |
Keywords: Small ruminants, gastrointestinal parasites, polymorphism |
topic |
Keywords: Small ruminants, gastrointestinal parasites, polymorphism Pequenos ruminantes, parasitos gastrintestinais, polimorfismo CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.por.fl_str_mv |
Pequenos ruminantes, parasitos gastrintestinais, polimorfismo |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
The increase in anthelmintic resistance in gastrointestinal nematodes on small ruminants became a worldwide issue on the development of livestock. In order to assess the resistance scenario in that context in the Rio Grande do Norte region, in vivo and in vitro resistance tests were carried out in 30 rural properties using chemicals in the avermectin, salicylanides, benzimidazoles and imidatiazols groups. For the in vivo test, group I was used as control and four other experimental groups received oral medication, being group II given albendazole (10 mg/kg), group III levamisole (7.5 mg/kg), group IV closantel (5.0 mg/kg) and group V ivermectin (0.2 mg/kg). The nematode infection was determined using fecal egg count (FEC) essays and the efficiency of each treatment was analyzed by the fecal egg count reduction test (FECRT). For the in vivo e in vitro tests, using tiabendazole, the necessary concentration to inhibit 50% of egg hatching (EC50) and assays with a 50% inhibition of larval development (LD50) were assessed, however 50% of larval development and migration inhibition assays (LD50) essays were carried out using ivermectin. For the molecular analysis Haemonchus contortus/property were isolated for the calculation of the frequency of resistant and sensitive alleles for single nucleotide polymorphisms (SNPs): F220Y, F167Y and E198A. The results obtained from in vivo tests showed that resistance was detected for all of the drugs, with leavamisole and closantel appearing as the most effective treatments with FECRT results of 84.3% and 74.6%, respectively. In the in vitro assays, tiabendazole had a result in the hatching test of EC50 0.598 µg/ml (95% IC: 0.323-0.818), while exhibiting in the larval development test a LD50 and LD99 0.518 µg/ml(95% IC: 0.432-0,887) and 4.359 µg/ml (95% IC: 3.047-8.178), respectively. For ivermectin in the larval development test, DL50 0.637 µg/ml(95% IC: 0.382 0.903 µg/ml) e DL99 19.56 µg/ml(95% IC: 11.44 46.55 µg/ml) was observed, while in the migration essay the LD50 value was 16.337 µM (95% IC: 12.7-21.3 µM). In the molecular analysis a higher frequency of the resistant allele for SNP F200Y was observed, presenting higher percentage values of 85.81% and 73.11%, while the F167Y resistant alleles had lower values ranging from 2.93% to 43.73%. In the E198A SNP, only sensitive alleles were detected. Assessing the scenario of anthelmintic resistance in the region, one notices that it s established for the main existing drugs on the market |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-11-14 |
dc.date.available.fl_str_mv |
2015-03-06 |
dc.date.accessioned.fl_str_mv |
2016-08-15T20:27:55Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
BEZERRA, Ana Carla Diógenes Suassuna. Anthelmintic resistance characterization and molecular analysis of Haemonchus contortus populations upon benzimidazoles in the city of Mossoró RN. 2014. 92 f. Tese (Doutorado em Sanidade e Produção Animal) - Universidade Federal Rural do Semi-Árido, Mossoró, 2014. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufersa.edu.br/handle/tede/310 |
identifier_str_mv |
BEZERRA, Ana Carla Diógenes Suassuna. Anthelmintic resistance characterization and molecular analysis of Haemonchus contortus populations upon benzimidazoles in the city of Mossoró RN. 2014. 92 f. Tese (Doutorado em Sanidade e Produção Animal) - Universidade Federal Rural do Semi-Árido, Mossoró, 2014. |
url |
https://repositorio.ufersa.edu.br/handle/tede/310 |
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por |
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por |
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CC-BY-SA info:eu-repo/semantics/openAccess |
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CC-BY-SA |
eu_rights_str_mv |
openAccess |
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application/pdf |
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Universidade Federal Rural do Semi-Árido |
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Programa de Pós-Graduação em Ciência Animal |
dc.publisher.initials.fl_str_mv |
UFERSA |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Centro de Ciências Agrárias - CCA |
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Universidade Federal Rural do Semi-Árido |
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