Efeito antioxidante, hepatoprotetor e genoprotetor da geoprópolis da abelha partamona cupira produzida no semiárido nordestino
Main Author: | |
---|---|
Publication Date: | 2018 |
Format: | Doctoral thesis |
Language: | por |
Source: | Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) |
Download full: | https://repositorio.ufersa.edu.br/handle/prefix/3740 |
Summary: | Hepatic pathologies are among the world's greatest health problems. Thus, there is a growing interest in studies related to the hepatoprotective effect of natural products, objectifying develop new drugs in order to increase the chances of greater efficiency and to offer a variety of medicines. With a complex chemical composition and rich in phenolic compounds, the propolis produced by Partamona cupira exhibits a large spectrum of biological activities, among them the high antioxidant activity stands out, with notable anti-inflammatory, antibacterial and antineoplastic effects. Some studies show that just like propolis produced by bees of the Apis genus, the propolis produced by indigenous stingless bees also has high biological activity. Therefore, the objective of the present study was to evaluate the hepatoprotective effect of the hydroethanolic extracts of the geopropolis produced by the cupira bee (Partamona cupira), obtained from the beekepers of the semiarid region of Rio Grande do Norte. For that, the chemical composition and antioxidant activity of the propolis were evaluated; as well as the hepatoprotective potential, through the induction of experimental cirrhosis in rats; and also to the in vitro evaluation of the antineoplastic potential of propolis in a human hepatic carcinoma (HepG2) cell line, and as the potential genotype in normal fibroblasts cell line (L929) exposed to hydrogen peroxide (H2O2). The samples from geopropolis presented 20 phenolic compounds, being phenols and flavonoids. The geopropolis extract of the cupira bee did not show cytotoxicity in normal cells and showed antigenotoxic or genoprotective effect, with percentages of genotoxicity reduction of 92.2%, 74.5% and 46.1%, respectively, for the concentrations of 500, 250 and 100μl / mL of the extract. The results of serum levels of hepatic injury indicative enzymes, indicated that the hydroalcoholic extract of the geopropolis of the cupira bee did not cause hepatic toxicity and had a hepatoprotective effect against the hepatic injury induced by thioacetamide (TAA). Through the macroscopic and histological evaluation of livers, it is also possible to suggest that the extract of the propolis analyzed exerted a hepatoprotective effect on the severity of cirrhosis, since in the livers of the animals treated with extract it was possible to verify that the regenerative nodules characteristic of cirrhosis were smaller and more discrete, when compared to the animals of the cirrhotic group. Likewise, there was a significant reduction in the percentage of collagen in the treated group compared to the cirrhotic group, demonstrating the hepatoprotective effect of the geopropolis of the honey bee, which was able to reduce the damage to the hepatocytes, although no statistical difference was observed in the weight of the livers in these groups, the stereological analysis showed that there was a significant difference in the percentage of hepatocytes and collagens among these groups. Thus, it is concluded that the extract of the geopropolis of the cupira bee presents varied chemical composition and promising pharmacological activities, highlighting the antioxidant, non-cytotoxic, genoprotective and hepatoprotective, since it exerted a hepatoprotective effect through the hepatic lesion induced by thioacetamide |
id |
UFER_b9aa82cbbc4c0cdc231643cd5a493669 |
---|---|
oai_identifier_str |
oai:repositorio.ufersa.edu.br:prefix/3740 |
network_acronym_str |
UFER |
network_name_str |
Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) |
repository_id_str |
|
spelling |
Efeito antioxidante, hepatoprotetor e genoprotetor da geoprópolis da abelha partamona cupira produzida no semiárido nordestinoGeoprópolisPartamona cupiraAtividade biológicaHepatoprotetoraCirrosePartamona cupiraGeopropolisBiological activityHepatoprotectiveCirrhosisCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIAHepatic pathologies are among the world's greatest health problems. Thus, there is a growing interest in studies related to the hepatoprotective effect of natural products, objectifying develop new drugs in order to increase the chances of greater efficiency and to offer a variety of medicines. With a complex chemical composition and rich in phenolic compounds, the propolis produced by Partamona cupira exhibits a large spectrum of biological activities, among them the high antioxidant activity stands out, with notable anti-inflammatory, antibacterial and antineoplastic effects. Some studies show that just like propolis produced by bees of the Apis genus, the propolis produced by indigenous stingless bees also has high biological activity. Therefore, the objective of the present study was to evaluate the hepatoprotective effect of the hydroethanolic extracts of the geopropolis produced by the cupira bee (Partamona cupira), obtained from the beekepers of the semiarid region of Rio Grande do Norte. For that, the chemical composition and antioxidant activity of the propolis were evaluated; as well as the hepatoprotective potential, through the induction of experimental cirrhosis in rats; and also to the in vitro evaluation of the antineoplastic potential of propolis in a human hepatic carcinoma (HepG2) cell line, and as the potential genotype in normal fibroblasts cell line (L929) exposed to hydrogen peroxide (H2O2). The samples from geopropolis presented 20 phenolic compounds, being phenols and flavonoids. The geopropolis extract of the cupira bee did not show cytotoxicity in normal cells and showed antigenotoxic or genoprotective effect, with percentages of genotoxicity reduction of 92.2%, 74.5% and 46.1%, respectively, for the concentrations of 500, 250 and 100μl / mL of the extract. The results of serum levels of hepatic injury indicative enzymes, indicated that the hydroalcoholic extract of the geopropolis of the cupira bee did not cause hepatic toxicity and had a hepatoprotective effect against the hepatic injury induced by thioacetamide (TAA). Through the macroscopic and histological evaluation of livers, it is also possible to suggest that the extract of the propolis analyzed exerted a hepatoprotective effect on the severity of cirrhosis, since in the livers of the animals treated with extract it was possible to verify that the regenerative nodules characteristic of cirrhosis were smaller and more discrete, when compared to the animals of the cirrhotic group. Likewise, there was a significant reduction in the percentage of collagen in the treated group compared to the cirrhotic group, demonstrating the hepatoprotective effect of the geopropolis of the honey bee, which was able to reduce the damage to the hepatocytes, although no statistical difference was observed in the weight of the livers in these groups, the stereological analysis showed that there was a significant difference in the percentage of hepatocytes and collagens among these groups. Thus, it is concluded that the extract of the geopropolis of the cupira bee presents varied chemical composition and promising pharmacological activities, highlighting the antioxidant, non-cytotoxic, genoprotective and hepatoprotective, since it exerted a hepatoprotective effect through the hepatic lesion induced by thioacetamidePatologias hepáticas estão entre os maiores problemas de saúde de ordem mundial. Sendo assim, cresce o interesse por estudos relacionados ao efeito hepatoprotetor de produtos naturais, objetivando desenvolver novas drogas com proposito de ampliar as chances de uma maior eficiência e disponibilizar uma variedade de medicamentos. Com uma composição química complexa e rica em compostos fenólicos, a própolis produzida por Partamona cupira exibe um amplo espectro de atividades biológicas, dentre as quais se destaca a alta atividade antioxidante, com notórios efeitos anti-inflamatório, antibacteriano e antineoplásico. Assim como a própolis produzida por abelhas do gênero Apis, a própolis produzida pelas abelhas indígenas sem ferrão possui alta atividade biológica. Dessa forma, a presente pesquisa teve por objetivos avaliar o efeito hepatoprotetor dos extratos hidroetanólico da geoprópolis produzida pela abelha cupira (Partamona cupira), obtido junto à meliponicultores do semiárido do Rio Grande do Norte. Para tanto, foram avaliados a composição química e atividade antioxidante da própolis da referida abelha; bem como o potencial hepatoprotetor, através da indução da cirrose experimental em ratos; além da avaliação in vitro do potencial antineoplásico da própolis em linhagem de células de carcinoma hepático humano (HepG2) e também o potencial genoprotetor em linhagem de células normais de fibroblastos (L929), expostas ao peróxido de hidrogênio (H2O2). As amostras da geoprópolis apresentaram 20 compostos fenólicos, sendo fenóis e flavonoides. O extrato da geoprópolis da abelha cupira não apresentou citotoxicidade em células normais e apresentou efeito antigenotóxico ou genoprotetor, com percentuais de redução de genotoxicidade de 92,2%, 74,5% e 46,1%, respectivamente, para as concentrações de 500, 250 e 100μl/mL do extrato. Os resultados dos níveis séricos das enzimas indicadoras de lesão hepática, indicaram que o extrato hidroalcoólico da geopropolis da abelha cupira não causou toxicidade hepática e exerceu efeito hepatoprotetor frente ao dano hepático induzido pela tioacetamida (TAA). Através da avaliação macroscópica e histológica dos fígados é possível também sugerir que o extrato da própolis analisado exerceu efeito hepatoprotetor na gravidade da cirrose, uma vez que nos fígados dos animais do grupo tratados com extrato foi possível constatar que os nódulos regenerativos característicos da cirrose foram menores e mais discretos, quando comparados aos dos animais do grupo cirrótico. Da mesma forma, houve redução significativa do percentual de colágeno do grupo tratado em relação ao grupo cirrótico, demonstrando o efeito hepatoprotetor da geoprópolis da abelha cupira, o qual foi capaz de reduzir o dano aos hepatócitos, embora não tenha sido verificada diferença estatística do peso dos fígados nos referidos grupos, a análise estereológica demonstrou que houve diferença significativa no percentual de hepatócitos e de colágenos entres esses grupos. Assim, conclui-se que o extrato da geoprópolis da abelha cupira apresenta composição química variada e promissoras atividades farmacológicas, destacando-se a antioxidante, não citotóxica, genoprotetora e hepatoprotetora, visto que exerceu efeito hepatoprotetor mediante a lesão hepática induzida pela tioacetamidaTrabalho não financiado por agência de fomento, ou autofinanciadoUniversidade Federal Rural do Semi-ÁridoBrasilCentro de Ciências Agrárias - CCAUFERSAPrograma de Pós-Graduação em Ciência AnimalMoura, Carlos Eduardo Bezerra de03597959431http://lattes.cnpq.br/4717410137206021Batista, Jael Soares68493193372http://lattes.cnpq.br/4937343270124186Costa, Wirton Peixoto02866148479http://lattes.cnpq.br/3442520902027224Coelho, Wesley Adson Costa01080935495http://lattes.cnpq.br/8362491439074916Costa, Kizzy Millenn de Freitas Mendonça2020-01-28T22:01:24Z2019-05-212020-01-28T22:01:24Z2018-11-13info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfCitação com autor incluído no texto: Costa (2018) Citação com autor não incluído no texto: (COSTA, 2018)https://repositorio.ufersa.edu.br/handle/prefix/3740porCOSTA, Kizzy Millenn de Freitas Mendonça. Efeito antioxidante, hepatoprotetor e genoprotetor da geoprópolis da abelha partamona cupira produzida no semiárido nordestino. 2018. 93 f. Tese (Doutorado em Ciência Animal), Universidade Federal Rural do Semi-Árido, Mossoró, 2018.CC-BY-SAinfo:eu-repo/semantics/openAccessreponame:Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU)instname:Universidade Federal Rural do Semi-Árido (UFERSA)instacron:UFERSA2023-10-30T20:27:17Zoai:repositorio.ufersa.edu.br:prefix/3740Repositório Institucionalhttps://repositorio.ufersa.edu.br/PUBhttps://repositorio.ufersa.edu.br/server/oai/requestrepositorio@ufersa.edu.br || admrepositorio@ufersa.edu.bropendoar:2023-10-30T20:27:17Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) - Universidade Federal Rural do Semi-Árido (UFERSA)false |
dc.title.none.fl_str_mv |
Efeito antioxidante, hepatoprotetor e genoprotetor da geoprópolis da abelha partamona cupira produzida no semiárido nordestino |
title |
Efeito antioxidante, hepatoprotetor e genoprotetor da geoprópolis da abelha partamona cupira produzida no semiárido nordestino |
spellingShingle |
Efeito antioxidante, hepatoprotetor e genoprotetor da geoprópolis da abelha partamona cupira produzida no semiárido nordestino Costa, Kizzy Millenn de Freitas Mendonça Geoprópolis Partamona cupira Atividade biológica Hepatoprotetora Cirrose Partamona cupira Geopropolis Biological activity Hepatoprotective Cirrhosis CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Efeito antioxidante, hepatoprotetor e genoprotetor da geoprópolis da abelha partamona cupira produzida no semiárido nordestino |
title_full |
Efeito antioxidante, hepatoprotetor e genoprotetor da geoprópolis da abelha partamona cupira produzida no semiárido nordestino |
title_fullStr |
Efeito antioxidante, hepatoprotetor e genoprotetor da geoprópolis da abelha partamona cupira produzida no semiárido nordestino |
title_full_unstemmed |
Efeito antioxidante, hepatoprotetor e genoprotetor da geoprópolis da abelha partamona cupira produzida no semiárido nordestino |
title_sort |
Efeito antioxidante, hepatoprotetor e genoprotetor da geoprópolis da abelha partamona cupira produzida no semiárido nordestino |
author |
Costa, Kizzy Millenn de Freitas Mendonça |
author_facet |
Costa, Kizzy Millenn de Freitas Mendonça |
author_role |
author |
dc.contributor.none.fl_str_mv |
Moura, Carlos Eduardo Bezerra de 03597959431 http://lattes.cnpq.br/4717410137206021 Batista, Jael Soares 68493193372 http://lattes.cnpq.br/4937343270124186 Costa, Wirton Peixoto 02866148479 http://lattes.cnpq.br/3442520902027224 Coelho, Wesley Adson Costa 01080935495 http://lattes.cnpq.br/8362491439074916 |
dc.contributor.author.fl_str_mv |
Costa, Kizzy Millenn de Freitas Mendonça |
dc.subject.por.fl_str_mv |
Geoprópolis Partamona cupira Atividade biológica Hepatoprotetora Cirrose Partamona cupira Geopropolis Biological activity Hepatoprotective Cirrhosis CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
topic |
Geoprópolis Partamona cupira Atividade biológica Hepatoprotetora Cirrose Partamona cupira Geopropolis Biological activity Hepatoprotective Cirrhosis CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
Hepatic pathologies are among the world's greatest health problems. Thus, there is a growing interest in studies related to the hepatoprotective effect of natural products, objectifying develop new drugs in order to increase the chances of greater efficiency and to offer a variety of medicines. With a complex chemical composition and rich in phenolic compounds, the propolis produced by Partamona cupira exhibits a large spectrum of biological activities, among them the high antioxidant activity stands out, with notable anti-inflammatory, antibacterial and antineoplastic effects. Some studies show that just like propolis produced by bees of the Apis genus, the propolis produced by indigenous stingless bees also has high biological activity. Therefore, the objective of the present study was to evaluate the hepatoprotective effect of the hydroethanolic extracts of the geopropolis produced by the cupira bee (Partamona cupira), obtained from the beekepers of the semiarid region of Rio Grande do Norte. For that, the chemical composition and antioxidant activity of the propolis were evaluated; as well as the hepatoprotective potential, through the induction of experimental cirrhosis in rats; and also to the in vitro evaluation of the antineoplastic potential of propolis in a human hepatic carcinoma (HepG2) cell line, and as the potential genotype in normal fibroblasts cell line (L929) exposed to hydrogen peroxide (H2O2). The samples from geopropolis presented 20 phenolic compounds, being phenols and flavonoids. The geopropolis extract of the cupira bee did not show cytotoxicity in normal cells and showed antigenotoxic or genoprotective effect, with percentages of genotoxicity reduction of 92.2%, 74.5% and 46.1%, respectively, for the concentrations of 500, 250 and 100μl / mL of the extract. The results of serum levels of hepatic injury indicative enzymes, indicated that the hydroalcoholic extract of the geopropolis of the cupira bee did not cause hepatic toxicity and had a hepatoprotective effect against the hepatic injury induced by thioacetamide (TAA). Through the macroscopic and histological evaluation of livers, it is also possible to suggest that the extract of the propolis analyzed exerted a hepatoprotective effect on the severity of cirrhosis, since in the livers of the animals treated with extract it was possible to verify that the regenerative nodules characteristic of cirrhosis were smaller and more discrete, when compared to the animals of the cirrhotic group. Likewise, there was a significant reduction in the percentage of collagen in the treated group compared to the cirrhotic group, demonstrating the hepatoprotective effect of the geopropolis of the honey bee, which was able to reduce the damage to the hepatocytes, although no statistical difference was observed in the weight of the livers in these groups, the stereological analysis showed that there was a significant difference in the percentage of hepatocytes and collagens among these groups. Thus, it is concluded that the extract of the geopropolis of the cupira bee presents varied chemical composition and promising pharmacological activities, highlighting the antioxidant, non-cytotoxic, genoprotective and hepatoprotective, since it exerted a hepatoprotective effect through the hepatic lesion induced by thioacetamide |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-11-13 2019-05-21 2020-01-28T22:01:24Z 2020-01-28T22:01:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Citação com autor incluído no texto: Costa (2018) Citação com autor não incluído no texto: (COSTA, 2018) https://repositorio.ufersa.edu.br/handle/prefix/3740 |
identifier_str_mv |
Citação com autor incluído no texto: Costa (2018) Citação com autor não incluído no texto: (COSTA, 2018) |
url |
https://repositorio.ufersa.edu.br/handle/prefix/3740 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
COSTA, Kizzy Millenn de Freitas Mendonça. Efeito antioxidante, hepatoprotetor e genoprotetor da geoprópolis da abelha partamona cupira produzida no semiárido nordestino. 2018. 93 f. Tese (Doutorado em Ciência Animal), Universidade Federal Rural do Semi-Árido, Mossoró, 2018. |
dc.rights.driver.fl_str_mv |
CC-BY-SA info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
CC-BY-SA |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal Rural do Semi-Árido Brasil Centro de Ciências Agrárias - CCA UFERSA Programa de Pós-Graduação em Ciência Animal |
publisher.none.fl_str_mv |
Universidade Federal Rural do Semi-Árido Brasil Centro de Ciências Agrárias - CCA UFERSA Programa de Pós-Graduação em Ciência Animal |
dc.source.none.fl_str_mv |
reponame:Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) instname:Universidade Federal Rural do Semi-Árido (UFERSA) instacron:UFERSA |
instname_str |
Universidade Federal Rural do Semi-Árido (UFERSA) |
instacron_str |
UFERSA |
institution |
UFERSA |
reponame_str |
Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) |
collection |
Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) |
repository.name.fl_str_mv |
Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) - Universidade Federal Rural do Semi-Árido (UFERSA) |
repository.mail.fl_str_mv |
repositorio@ufersa.edu.br || admrepositorio@ufersa.edu.br |
_version_ |
1809747440720412672 |