Ensaios de biodegradabilidade aeróbia de hidrocarbonetos derivados do petróleo em solos

• Autor(a) principal: Pedroti, Giselle Intra
• Data de Publicação: 2007
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)
• Texto Completo: http://repositorio.ufes.br/handle/10/3879
• topic: Biodegradação; Biorremediação; Hidrocarbonetos; Bactérias; Engenharia Sanitária; 628

The environmental pollution caused by oil and derivatives´ leaking is a great worry about environment. With the target of recovering the environment from the pollution caused by those accidents, this work intends to isolate and characterize microorganisms with capacity of petroleum biodegradation in ground environment. The research achieve 19 isolated, which were analyzed through manual system, based in Bartha respirometric method (modified), described by NBR 14.283 (1999), by literature through condutivimetrics, which determines microbiologic activity by carbonic gas generation and, indirectly, biodegradation of ground contaminant. The research was done in three experiences: in first, biodegradation capacity of 19 isolated strains was verified, with sandy and vermicuta as substracts. The second experience tested four strains (1, 2, 13 and 14) with four hydrocarbons (A, B, C and D), forming 16 matricial treatments, with sand and vermicuta as substracts, with the purpose of verify isolated strains specificity. The third experience involved two strains (1 and 14) with four hydrocarbons (A, B, C and D), in matricial form, using sandy and clay ground, with the purpose of verify possible differences of ground biodegradation. The evaluted strains showed biodegradation potential of petroleum derivatives hydrocarbons. The biodegradation analysis was done by carbonic gas generated by bacterias in respirometric system. Data were adjusted according with biodegradation rate calculations proposed by chemical kinetics. Values until 32% hydrocarbons biodegradation in a 15 day period were observed, considering the only biodegradation model proposed by Firme (2005). The biodegradation rate presented variation according with each type of organic composites and isolated strain utilized in each experience treatment.