Yacon (Smallanthus sonchifolius) na modulação do cancêr de cólon em modelo animal

• Autor(a) principal: Grancieri, Mariana
• Data de Publicação: 2016
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)
• Texto Completo: http://repositorio.ufes.br/handle/10/4942
• topic: Fructooligosaccharides; Endotoxemy; Intestinal permeability; Yacon flour; Immunology; Histology; Fruto-oligossacarídeos; Endotoxemia; Permeabilidade intestinal; Farinha de yacon; Ciência e Tecnologia de Alimentos; Farinhas; Imunologia; Histologia; 664

Colorectal cancer (CC) can induce clinical complications and even death of the patient. The yacon flour (FY) (Smallanthus sonchifolius) is rich in fructooligosaccharides (FOS) that are fermented by bifidobacteria producing beneficial effects on intestinal health. Its effect on the CC, however, is still unknown. The objective of this study was to investigate the intestinal and immunological effects of supplementation with yacon flour in animal model of induced colon cancer. Male Wistar rats were divided into groups S (without CC and without FY, n=10), C group (with CC and without FY, n=12), Y group (without CC and with FY,n=10) and CY group (with CC and FY, n=12). For five weeks, the C and CY groups received, subcutaneously, a weekly dose of 25 mg / kg weight of 1, 2-dimethylhydrazine (DMH) drug, followed by eight weeks for the development of CC. During the first 13 weeks of the study, all groups received standard commercial ration. After this period, the Y and CY groups consumed diet FY in amounts sufficient to provide 7.5% of FOS for eight weeks and the other groups consume AIN-93M diet. After the experimental period were determined: intestinal permeability, intraluminal pH, endotoxemia, aberrant crypt foci (ACF), qualitative histology (apical diameter, baseline diameter and crypt depth, beyond the crypts count per field) levels of anion superoxide and nitric oxide and plasma total antioxidant capacity (TAC). The data were analyzed by two-way ANOVA (analysis of variance) followed by Newman-Keuls test (p <0.05). Yacon flour exerts beneficial and significant effect on intestinal permeability, given that the urinary excretion of lactulose and mannitol was lower in Y and CY groups compared to control groups (S and C). Furthermore, there was a positive action in reducing the intraluminal pH, the percentage reduction in FCA levels, in addition to increased number and depth of the colonic crypts, TAC and oxidative metabolism via the production of superoxide anion. But it was not observed effectiveness of yacon flour on the number of ACF in endotoxemia and nitric oxide release. It may be concluded that the FY is effective and beneficial in the modulation of colorectal cancer in animal model, however, further studies are required to elucidate the mechanisms by which this occurs.