Efeito da administração oral de Rapamicina sobre a reatividade vascular de camundongos ateroscleróticos

• Autor(a) principal: Gadioli, Adriana Lários Nóbrega
• Data de Publicação: 2007
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)
• Texto Completo: http://repositorio.ufes.br/handle/10/7927
• topic: Rapamicina; Função endotelial; Reatividade vascular; Camundongo ApoE Knockout; Fisiologia; Aterosclerose; Endotélio vascular; Camundongo; 612

Post-coronary angioplasty restenosis has been controlled through the use of rapamycin eluting stents. However, different reports suggest that rapamycin may have deleterious effects on endothelial function. We evaluated in vitro the effects of oral administration of rapamycin on endothelial function in mesenteric arteriolar bed of eight weeks old apolipoprotein E Knockout mice. Contractility to norepinephrine (NE) and relaxation to acetylcholine (ACh) or sodium nitropusside (SN) were evaluated twelve weeks after administration rapamycin (5mg/kg/day). In mesenteric arteriolar bed, rapamycin did not change the vascular contractility to NE in the rapamycin group (116 ± 3, 7%, n=10; p=NS) compared to the control group (114 ± 14%, n=0,9; p=NS). The relaxant vascular reactivity to ACh was similar in rapamycin group (49 ± 5%, n=10; p=NS) and control group (53 ± 4, 4%, n=0,9; p=NS). The maximum vascular relaxation induced by SN, was similar rapamycin group (74 ± 5%, n=10; p=NS) and in the control group (74 ± 6%, n=09; p=NS). Rapamycin treatment was associated with an increased plasma total cholesterol in the rapamycin group (941 ± 63 mg/dL, n = 12; p= < 0, 05) compared with the control group (721 ± 49 mg/dL, n = 7; p= < 0, 05). The aortic plaque area in mice fed with oral rapamycin was significantly decreased when compared to control group (6.946±2.276 vs. 46.156±13.028 µm 2 , N=2; p<0, 01). The administration of high concentrations of RP (5mg/kg) does not modify ApoE mouse mesenteric arteriolar tone.