Estudos fitoquímico, alelopático, tóxico e mutagênico de Erythrina mulungu MART. ex BENTH. utilizando bioensaios

• Autor(a) principal: De Bona, Ana Paula
• Data de Publicação: 2009
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)
• Texto Completo: http://repositorio.ufes.br/handle/10/9981
• topic: Allelopathy; Mutagenesis; Cytotoxicity; Phytochemica; Micronucleus test in rodents; Citotoxicidade; Fitoquímica; Teste de micronúcleo em roedores; Erythrina mulungu (Mart. ex Benth.); DL50; Allium cepa; Agronomia; Mutagênese; Química vegetal; Cebola; Alelopatia; 57

The plants produce a range of secondary compounds, which can act as active principle in drugs or can produce toxic effect on animals and other plants. Thus, as the Erythrina mulungu (Mart. ex Benth.) a kind of pharmacological and ecological importance, this study aims to evaluate the structure phytochemistry and allelopathic and mutagenic effects potential of the crude extract of the inflorescences and leaves of this plant through of different bioassays. The phytochemical survey was conducted through chemical reactions that resulted in the development of color and/or precipitate characteristic. The allelopathic tests in Allium cepa were conducted and consisted of 8 treatments (distilled water and 0025; 0.05, 0.1, 0.2, 0.3, 0.4 and 0.6 mg/mL of extracts) and 5 repetitions with 20 seeds each. Evaluated the germination speed, the first count, the germination percentage and the media root. For analysis cytotoxic and mutagenic in plants, seeds of Allium cepa were subjected to continuous and discontinuous treatment (20 and 72 hours) and assessed the mitotic index, aneugenic effect index and clastogenic effect index. The acute toxicity in animals was achieved through the establishment of the median lethal dose (LD50) and cytotoxic and mutagenic analysis made by the micronucleus test, using the method of smear, after exposure of animals to 5 days of treatment. The results show the presence of reducing sugars, phenols and tannins, proteins and amino acids, alkaloids, flavonoids, depsides and depsidones, derived from coumarin in both organs; saponins, steroids and triterpenes in the leaves and cardiac and anthraquinones glycosides in inflorescences. The bodies have allelopathic activity on the root development, potential cytotoxic and no aneugenic effect on the onion. The inflorescences have potential clastogenic. To the LD50, the leave showed non toxic and inflorescence, moderately toxic. Both showed lack of cytotoxic and genotoxic potential in cells of rodents. The use ofE. mulungu, both for the management of degraded or agricultural areas, as for the manufacture of medicines, must be done so cautiously