Relação do fluxo de íons (H+) em raízes com as atividades alelopáticas e mutagênicas do extrato etanólico e da nanodispersão de Mikania glomerata Spreng.

• Autor(a) principal: Barcelos, Monique Ellen Farias
• Data de Publicação: 2019
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)
• Texto Completo: http://repositorio.ufes.br/handle/10/11406
• topic: Protons; Cytotoxicity; Allelopathic; Prótons; Allium cepa; Agronomia; Mutagênese; Alelopatia; Cebola; Alface; Nanopartículas; Mikania; 57

Mikania glomerata Spreng is a very studied species on the ethnopharmacological aspect and production of secondary compounds, such as coumarins. These compounds have been associated with the use of nanoparticles which are used to associate plant-active compounds, that are poorly soluble, increasing the bioavailability of these. Thus, the present study aims to evaluate the ionic capacity of the extract and nanodispersion of the content of coumarin (0.001; 0.002; 0.004; 0.008 and 0.016 mg/mL) of Mikania, relating this ability with a potential allelopathic and mutagenic activity in Roots of Lactuca sativa and Allium Cepa. The H + flow was determined in the root elongation zone using selective ion vibratory system. Allelopathic activity was evaluated by the germination test. After its protrusion, the roots were photographed and analyzed in ImageJ software. To evaluate the mutagenic activity, the seeds were germinated on Petri dishes in triplicate and received 5mL of Mikania extract in the same concentrations used for nanodispersion, in addition to the groups: pure Coumarin 0.002 mg/mL (CuP); Positive control methylmethosulfonate at 4x10-4M (MMS)-as positive control-and negative control (distilled water). After root protrusion, slides were made for analysis of the mitotic index and possible chromosomal abnormalities. The data obtained were submitted to ANOVA and test of averages (p < 0.05). The flow of H +, in the dose-response curve, indicated the concentration of 0.002 mg/mL, being the best concentration for comparison to the other treatments. It was also possible to observe that all treatments promoted inhibition of the H + flow when compared to the negative control. Similarly, it was found in the flow of coumarin content in nanodispersion, in which it was possible to evidence an oscillation, according to the concentrations analyzed. This result may be related to the inhibition of seed germination, abnormal cell differentiation and reduction of plant growth. In the analysis of allelopathic activity, it was observed that Lactuca sativa showed a higher decrease in the percentage of germination and a more expressive increase in the index of Allelpathy, when compared to Allium Cepa, at the concentration of 0, 004mg/mL of coumarin content in the extract. However, for the test with nanodispersion, it was evidenced that, in both testing organisms, there was a significant drop in germination and/or inhibition of germination, when compared to control, providing greater allelopathic activity. As for the mutagenic activity, in continuous treatment, the CuP promoted a greater clastogenic effect, indicating that the isolated coumarin promotes more damage to the plant cell than when associated with other compounds present in the Guaco extract. In the discontinuous treatment of the extract, it was possible to verify that, for 24h, 48h, and for the treatment of recovery, there was a decrease in the mitotic index. In the 24h treatment, the concentration of 0, 001mg/mL had a clastogenic effect when compared to the negative control.