Avaliação do metabolismo energético in vitro de formas epimastigotas de trypanossoma cruzi pré e pós tratamento específico com benzonidazol
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/8204 |
Resumo: | The Chagas disease is a zoonosis of huge worldwide impact that has such as vector triatomine insects belonging to the hemiptera order and such as etiological agent Trypanosoma cruzi a flagellate that presents different evolutive manners. The evolutive manners of Trypanosoma cruzi can gain energy from various sources, for example glucose and amino acids. This capacity in gain energy from various sources lead to understand how this parasite can be able to adapt and survive in different environment, be it on the vector insects or on the vertebrate host. The aim of this work was evaluate the energetic metabolism in vitro of epimastigote forms of Trypanosoma cruzi pre and post-treatment with different concentrations of benznidazole. The parasites were grown in means of Liver infusion triptose - LIT and the organic acids referring to them energetic metabolism were measured for 3º, 6º, 9º e 12º days of growing. For evaluation of the drug effect on the parasite's metabolism was added in the growing medium the following concentrations: 100 μmolar, 50 μmolar, 25 μmolar, 12.5 μmolar e 6.25 μmolar. It was possible to detect organic acids referring to glycolytic path, oxidation of fatty acids, urea cycle and citric acid of parasites in every analyzed day. It was possible to observe that the largest concentrations of drug (25, 50 e 100μmolar) induced anaerobic way of energy production. This way before the non-detection of glicossomais and mitochondrias final products, it can conclude that one of the mechanisms of action of this drug is to prevent the aerobic metabolism of the parasite, inhibiting mitochondria paths and glicossomais. |
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Castro, Ana Maria dehttp://lattes.cnpq.br/9232309971000621Vinaud, Marina Clarehttp://lattes.cnpq.br/1921551651088660Alves, Daniella de Sousa Mendes MoreiraCosta, Tatiane Luiza dahttp://lattes.cnpq.br/1300952549163071Nogueira, Kamilla Soares2018-03-07T12:13:12Z2015-08-12NOGUEIRA, Kamilla Soares. Avaliação do metabolismo energético in vitro de formas epimastigotas de trypanossoma cruzi pré e pós tratamento específico com benzonidazol. 2015. 59 f. Dissertação (Mestrado em Biologia da Relação Parasito-Hospedeiro (IPTSP)) - Universidade Federal de Goiás, Goiânia, 2015.http://repositorio.bc.ufg.br/tede/handle/tede/8204The Chagas disease is a zoonosis of huge worldwide impact that has such as vector triatomine insects belonging to the hemiptera order and such as etiological agent Trypanosoma cruzi a flagellate that presents different evolutive manners. The evolutive manners of Trypanosoma cruzi can gain energy from various sources, for example glucose and amino acids. This capacity in gain energy from various sources lead to understand how this parasite can be able to adapt and survive in different environment, be it on the vector insects or on the vertebrate host. The aim of this work was evaluate the energetic metabolism in vitro of epimastigote forms of Trypanosoma cruzi pre and post-treatment with different concentrations of benznidazole. The parasites were grown in means of Liver infusion triptose - LIT and the organic acids referring to them energetic metabolism were measured for 3º, 6º, 9º e 12º days of growing. For evaluation of the drug effect on the parasite's metabolism was added in the growing medium the following concentrations: 100 μmolar, 50 μmolar, 25 μmolar, 12.5 μmolar e 6.25 μmolar. It was possible to detect organic acids referring to glycolytic path, oxidation of fatty acids, urea cycle and citric acid of parasites in every analyzed day. It was possible to observe that the largest concentrations of drug (25, 50 e 100μmolar) induced anaerobic way of energy production. This way before the non-detection of glicossomais and mitochondrias final products, it can conclude that one of the mechanisms of action of this drug is to prevent the aerobic metabolism of the parasite, inhibiting mitochondria paths and glicossomais.A doença de Chagas é uma zoonose de grande impacto mundial que tem como vetor insetos triatomínios pertencentes à ordem hemíptera e como agente etiológico Trypanosoma cruzi um flagelado que apresenta diferentes formas evolutivas. As formas evolutivas de Trypanosma cruzi podem obter energia de diversas fontes, como por exemplo glicose e aminoácidos. Essa capacidade em obter energia de várias fontes leva a entender como este parasito consegue se adaptar e sobreviver em diferentes ambientes seja ele no inseto vetor ou no hospedeiro vertebrado. O objetivo deste trabalho foi avaliar o metabolismo energético in vitro de formas epimastigotas de Trypanosoma cruzi pré e pós tratamento com diferentes concentrações de benzonidazol. Os parasitos foram cultivados em meio Liver infusion triptose - LIT e os ácidos orgânicos referentes ao seu metabolismo energético foram quantificados aos 3º, 6º, 9º e 12º dias de cultura. Para avaliação do efeito do fármaco no metabolismo do parasito foi adicionado ao meio de cultura às seguintes concentrações: 100 μmolar, 50 μmolar, 25μmolar, 12.5 μmolar e 6.25μmolar. Foi possível detectar ácidos orgânicos referentes à via glicolítica, oxidação de ácidos graxos, ciclo da uréia e do ácido cítrico nos parasitos em todos os dias analisados. Foi possível observar que as maiores concentrações do fármaco (25, 50 e 100μmolar) induziram a via anaeróbia de produção de energia. Desta forma, diante da não detecção de produtos finais glicossomais e mitocondriais pode-se concluir que um dos mecanismos de ação deste fármaco é impedir o metabolismo aeróbio do parasito, inibindo vias mitocondriais e glicossomais.Submitted by Erika Demachki (erikademachki@gmail.com) on 2018-03-06T20:14:19Z No. of bitstreams: 2 Dissertação - Kamilla Soares Nogueira - 2015.pdf: 1269034 bytes, checksum: 57bf041ae63db9b0edaaf9382d4a5698 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2018-03-07T12:13:12Z (GMT) No. of bitstreams: 2 Dissertação - Kamilla Soares Nogueira - 2015.pdf: 1269034 bytes, checksum: 57bf041ae63db9b0edaaf9382d4a5698 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2018-03-07T12:13:12Z (GMT). No. of bitstreams: 2 Dissertação - Kamilla Soares Nogueira - 2015.pdf: 1269034 bytes, checksum: 57bf041ae63db9b0edaaf9382d4a5698 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2015-08-12Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Federal de GoiásPrograma de Pós-graduação em Biologia da Relação Parasito-Hospedeiro (IPTSP)UFGBrasilInstituto de Patologia Tropical e Saúde Pública - IPTSP (RG)http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessTrypanosoma cruziMetabolismoBenzonidazolMetabolismBenznidazoleCIENCIAS BIOLOGICAS::PARASITOLOGIAAvaliação do metabolismo energético in vitro de formas epimastigotas de trypanossoma cruzi pré e pós tratamento específico com benzonidazolIn vitro energetic metabolism evaluation of epimastigote forms of trypanosoma cruzi before and after specific treatment with benzonidazoleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-5087190859350688984600600600600-7769011444564556288-45445767472715743062075167498588264571reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; 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dc.title.eng.fl_str_mv |
Avaliação do metabolismo energético in vitro de formas epimastigotas de trypanossoma cruzi pré e pós tratamento específico com benzonidazol |
dc.title.alternative.eng.fl_str_mv |
In vitro energetic metabolism evaluation of epimastigote forms of trypanosoma cruzi before and after specific treatment with benzonidazole |
title |
Avaliação do metabolismo energético in vitro de formas epimastigotas de trypanossoma cruzi pré e pós tratamento específico com benzonidazol |
spellingShingle |
Avaliação do metabolismo energético in vitro de formas epimastigotas de trypanossoma cruzi pré e pós tratamento específico com benzonidazol Nogueira, Kamilla Soares Trypanosoma cruzi Metabolismo Benzonidazol Metabolism Benznidazole CIENCIAS BIOLOGICAS::PARASITOLOGIA |
title_short |
Avaliação do metabolismo energético in vitro de formas epimastigotas de trypanossoma cruzi pré e pós tratamento específico com benzonidazol |
title_full |
Avaliação do metabolismo energético in vitro de formas epimastigotas de trypanossoma cruzi pré e pós tratamento específico com benzonidazol |
title_fullStr |
Avaliação do metabolismo energético in vitro de formas epimastigotas de trypanossoma cruzi pré e pós tratamento específico com benzonidazol |
title_full_unstemmed |
Avaliação do metabolismo energético in vitro de formas epimastigotas de trypanossoma cruzi pré e pós tratamento específico com benzonidazol |
title_sort |
Avaliação do metabolismo energético in vitro de formas epimastigotas de trypanossoma cruzi pré e pós tratamento específico com benzonidazol |
author |
Nogueira, Kamilla Soares |
author_facet |
Nogueira, Kamilla Soares |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Castro, Ana Maria de |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/9232309971000621 |
dc.contributor.advisor-co1.fl_str_mv |
Vinaud, Marina Clare |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/1921551651088660 |
dc.contributor.referee1.fl_str_mv |
Alves, Daniella de Sousa Mendes Moreira |
dc.contributor.referee2.fl_str_mv |
Costa, Tatiane Luiza da |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/1300952549163071 |
dc.contributor.author.fl_str_mv |
Nogueira, Kamilla Soares |
contributor_str_mv |
Castro, Ana Maria de Vinaud, Marina Clare Alves, Daniella de Sousa Mendes Moreira Costa, Tatiane Luiza da |
dc.subject.por.fl_str_mv |
Trypanosoma cruzi Metabolismo Benzonidazol |
topic |
Trypanosoma cruzi Metabolismo Benzonidazol Metabolism Benznidazole CIENCIAS BIOLOGICAS::PARASITOLOGIA |
dc.subject.eng.fl_str_mv |
Metabolism Benznidazole |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::PARASITOLOGIA |
description |
The Chagas disease is a zoonosis of huge worldwide impact that has such as vector triatomine insects belonging to the hemiptera order and such as etiological agent Trypanosoma cruzi a flagellate that presents different evolutive manners. The evolutive manners of Trypanosoma cruzi can gain energy from various sources, for example glucose and amino acids. This capacity in gain energy from various sources lead to understand how this parasite can be able to adapt and survive in different environment, be it on the vector insects or on the vertebrate host. The aim of this work was evaluate the energetic metabolism in vitro of epimastigote forms of Trypanosoma cruzi pre and post-treatment with different concentrations of benznidazole. The parasites were grown in means of Liver infusion triptose - LIT and the organic acids referring to them energetic metabolism were measured for 3º, 6º, 9º e 12º days of growing. For evaluation of the drug effect on the parasite's metabolism was added in the growing medium the following concentrations: 100 μmolar, 50 μmolar, 25 μmolar, 12.5 μmolar e 6.25 μmolar. It was possible to detect organic acids referring to glycolytic path, oxidation of fatty acids, urea cycle and citric acid of parasites in every analyzed day. It was possible to observe that the largest concentrations of drug (25, 50 e 100μmolar) induced anaerobic way of energy production. This way before the non-detection of glicossomais and mitochondrias final products, it can conclude that one of the mechanisms of action of this drug is to prevent the aerobic metabolism of the parasite, inhibiting mitochondria paths and glicossomais. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-08-12 |
dc.date.accessioned.fl_str_mv |
2018-03-07T12:13:12Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
NOGUEIRA, Kamilla Soares. Avaliação do metabolismo energético in vitro de formas epimastigotas de trypanossoma cruzi pré e pós tratamento específico com benzonidazol. 2015. 59 f. Dissertação (Mestrado em Biologia da Relação Parasito-Hospedeiro (IPTSP)) - Universidade Federal de Goiás, Goiânia, 2015. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/8204 |
identifier_str_mv |
NOGUEIRA, Kamilla Soares. Avaliação do metabolismo energético in vitro de formas epimastigotas de trypanossoma cruzi pré e pós tratamento específico com benzonidazol. 2015. 59 f. Dissertação (Mestrado em Biologia da Relação Parasito-Hospedeiro (IPTSP)) - Universidade Federal de Goiás, Goiânia, 2015. |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/8204 |
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por |
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por |
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600 600 600 600 |
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2075167498588264571 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
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http://creativecommons.org/licenses/by-nc-nd/4.0/ |
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openAccess |
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Universidade Federal de Goiás |
dc.publisher.program.fl_str_mv |
Programa de Pós-graduação em Biologia da Relação Parasito-Hospedeiro (IPTSP) |
dc.publisher.initials.fl_str_mv |
UFG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG) |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
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