Estudo da obtenção de açúcares redutores totais a partir do bagaço de laranja (Citrus sinenses) por hidrólises ácida diluída e enzimática
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/6084 |
Resumo: | Due to the recent research for new fuels from renewable sources ethanol has been gaining momentum, because it can be produced from diverse raw materials, such as agro-industrial residues. The objective with this work was to study the production of reducing sugar by dilute acid, and enzymatic hydrolysis of the orange bagasse from juice industry. The orange bagasse was collected, cut into pieces, and crushed. Granulometry, and the contents of moisture, ashes, holocellulose, cellulose, hemicelulose, soluble and insoluble lignin were determined. The pre-treatment was done with calcium hydroxide following what was previously tested by Silva et al. (2013). A pre-test of the enzymes combination was done using 2 g of pre-treated biomass, in dry base, using 3 FPU/mL of cellulase, and 0 U/g and 3 U/g of xylanase. For the hydrolysis two central composite factorial 2³ designs were done, with the answer in total reducing sugars (ART). For the dilute acid hydrolysis the factors were HCl concentration, temperature, and time, and for the enzymatic the concentrations of cellulase and xylanase and time. The granulometry showed that 47.75% of the biomass with diameter over 0.833 mm, 32.84% of the biomass with an average diameter of 0.564 mm, and 19.41% of the biomass with diameter under 0.295 mm. The moisture content prior to drying was 84.69% and 7.38%, the ashes content was 3.79%. The cellulose content was 22.90% and the hemicellulose was 3.39%. The lignin content was 9.90%. The reducing sugar results for the acid hydrolysis varied from 9.32±0.68 mg ART per g of biomass to 30.15±0.31 mg ART per g biomass, the most significant factor was temperature, and the least was time. It was not possible to find the optimum region with the studied factors. The reducing sugar results for the enzymatic hydrolysis varied from 75.33±3.82 mg ART per g biomass to 99.66±0.62 mg ART per g biomass, the most significant factor was the cellulase concentration, and the least significant the xylanase concentration. The studied factors did not show the optimum region to maximize the reducing sugars content. The enzymatic hydrolysis produced larger concentrations of reducing sugars than the acid hydrolysis. |
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Seolatto, Araceli Aparecidahttp://lattes.cnpq.br/1495882445078650Freitas, Fernanda Ferreirahttp://lattes.cnpq.br/0883086742146577Seolatto, Araceli AparecidaFreitas, Fernanda FerreiraRosa, Paula Rúbia FerreiraSuarez, Carlos Alberto Galeanohttp://lattes.cnpq.br/3300018822284940Nogueira, Danielle Pires2016-09-01T14:42:33Z2016-04-18NOGUEIRA, D. P. Estudo da obtenção de açúcares redutores totais a partir do bagaço de laranja (Citrus sinenses) por hidrólises ácida diluída e enzimática. 2016. 74 f. Dissertação (Mestrado em Engenharia Química) - Universidade Federal de Goiás, Goiânia, 2016.http://repositorio.bc.ufg.br/tede/handle/tede/6084Due to the recent research for new fuels from renewable sources ethanol has been gaining momentum, because it can be produced from diverse raw materials, such as agro-industrial residues. The objective with this work was to study the production of reducing sugar by dilute acid, and enzymatic hydrolysis of the orange bagasse from juice industry. The orange bagasse was collected, cut into pieces, and crushed. Granulometry, and the contents of moisture, ashes, holocellulose, cellulose, hemicelulose, soluble and insoluble lignin were determined. The pre-treatment was done with calcium hydroxide following what was previously tested by Silva et al. (2013). A pre-test of the enzymes combination was done using 2 g of pre-treated biomass, in dry base, using 3 FPU/mL of cellulase, and 0 U/g and 3 U/g of xylanase. For the hydrolysis two central composite factorial 2³ designs were done, with the answer in total reducing sugars (ART). For the dilute acid hydrolysis the factors were HCl concentration, temperature, and time, and for the enzymatic the concentrations of cellulase and xylanase and time. The granulometry showed that 47.75% of the biomass with diameter over 0.833 mm, 32.84% of the biomass with an average diameter of 0.564 mm, and 19.41% of the biomass with diameter under 0.295 mm. The moisture content prior to drying was 84.69% and 7.38%, the ashes content was 3.79%. The cellulose content was 22.90% and the hemicellulose was 3.39%. The lignin content was 9.90%. The reducing sugar results for the acid hydrolysis varied from 9.32±0.68 mg ART per g of biomass to 30.15±0.31 mg ART per g biomass, the most significant factor was temperature, and the least was time. It was not possible to find the optimum region with the studied factors. The reducing sugar results for the enzymatic hydrolysis varied from 75.33±3.82 mg ART per g biomass to 99.66±0.62 mg ART per g biomass, the most significant factor was the cellulase concentration, and the least significant the xylanase concentration. The studied factors did not show the optimum region to maximize the reducing sugars content. The enzymatic hydrolysis produced larger concentrations of reducing sugars than the acid hydrolysis.Frente as recentes buscas por novos combustíveis de fontes renováveis o etanol de segunda geração tem ganhado força, por poder ser produzido a partir de diversas matérias primas lignocelulósicas, como os resíduos agroindustriais. Objetivou-se com este trabalho estudar a geração de açúcares redutores por hidrólises ácida diluída e enzimática do bagaço de laranja proveniente da indústria de suco. O bagaço de laranja foi coletado, cortado, seco e moído. Granulometria, umidade, cinzas, concentrações de holocelulose, celulose, hemicelulose e lignina solúvel e insolúvel foram determinadas. O pré-tratamento foi realizado com hidróxido de cálcio de acordo com o previamente testado por Silva et al. (2013). Foi realizado um pré-teste da combinação das enzimas. Para as hidrólises foram feitos dois planejamentos fatoriais do tipo composto central 2³, com resposta em açúcares redutores totais (ART). Para a hidrólise ácida diluída os fatores estudados foram concentração de HCl, temperatura e tempo de hidrólise, na enzimática as concentrações de celulase e hemicelulase e o tempo. Na análise granulométrica encontrou-se 47,75% da biomassa com diâmetro superior a 0,833 mm, 32,84% de biomassa com diâmetro médio de 0,564 mm e 19,41% com diâmetro inferior a 0,295 mm. A umidade da biomassa antes da secagem foi de 84,69%, depois da secagem de 7,38% e as cinzas 3,79%. O conteúdo de celulose foi de 22,90% e o de hemicelulose 3,39%. O conteúdo de lignina foi de 9,90%. Houve uma perda de massa média de 30,03% no pré-tratamento. O pré-teste indicou um efeito positivo na combinação das enzimas para geração de açúcares redutores. Os resultados de açúcares redutores da hidrólise ácida variaram de 9,32±0,68 mg ART por g de biomassa a 30,15±0,31 mg ART por g biomassa, o fator mais significativo foi a temperatura e o menos o tempo, não foi possível encontrar a região ótima com os fatores estudados. Os resultados de açúcares redutores da hidrólise enzimática variaram de 75,33±3,82 mg ART por g biomassa a 99,66±0,62 mg ART por g biomassa, o fator mais significativo foi a concentração de celulase e o menos a concentração de xilanase. Com os fatores estudados não foi possível encontrar a região ótima de geração de açúcares redutores. A hidrólise enzimática gerou maiores concentrações de açúcares redutores que a hidrólise ácida.Submitted by Luciana Ferreira (lucgeral@gmail.com) on 2016-09-01T14:42:20Z No. of bitstreams: 2 Dissertação - Danielle Pires Nogueira - 2016.pdf: 2016091 bytes, checksum: eb50931397d77906f88aaeec4a5fa5d0 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2016-09-01T14:42:33Z (GMT) No. of bitstreams: 2 Dissertação - Danielle Pires Nogueira - 2016.pdf: 2016091 bytes, checksum: eb50931397d77906f88aaeec4a5fa5d0 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2016-09-01T14:42:33Z (GMT). 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dc.title.por.fl_str_mv |
Estudo da obtenção de açúcares redutores totais a partir do bagaço de laranja (Citrus sinenses) por hidrólises ácida diluída e enzimática |
title |
Estudo da obtenção de açúcares redutores totais a partir do bagaço de laranja (Citrus sinenses) por hidrólises ácida diluída e enzimática |
spellingShingle |
Estudo da obtenção de açúcares redutores totais a partir do bagaço de laranja (Citrus sinenses) por hidrólises ácida diluída e enzimática Nogueira, Danielle Pires Planejamento composto central Xilanase Celulase Central composite design Xylanase Cellulase ENGENHARIAS::ENGENHARIA QUIMICA |
title_short |
Estudo da obtenção de açúcares redutores totais a partir do bagaço de laranja (Citrus sinenses) por hidrólises ácida diluída e enzimática |
title_full |
Estudo da obtenção de açúcares redutores totais a partir do bagaço de laranja (Citrus sinenses) por hidrólises ácida diluída e enzimática |
title_fullStr |
Estudo da obtenção de açúcares redutores totais a partir do bagaço de laranja (Citrus sinenses) por hidrólises ácida diluída e enzimática |
title_full_unstemmed |
Estudo da obtenção de açúcares redutores totais a partir do bagaço de laranja (Citrus sinenses) por hidrólises ácida diluída e enzimática |
title_sort |
Estudo da obtenção de açúcares redutores totais a partir do bagaço de laranja (Citrus sinenses) por hidrólises ácida diluída e enzimática |
author |
Nogueira, Danielle Pires |
author_facet |
Nogueira, Danielle Pires |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Seolatto, Araceli Aparecida |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/1495882445078650 |
dc.contributor.advisor-co1.fl_str_mv |
Freitas, Fernanda Ferreira |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/0883086742146577 |
dc.contributor.referee1.fl_str_mv |
Seolatto, Araceli Aparecida |
dc.contributor.referee2.fl_str_mv |
Freitas, Fernanda Ferreira |
dc.contributor.referee3.fl_str_mv |
Rosa, Paula Rúbia Ferreira |
dc.contributor.referee4.fl_str_mv |
Suarez, Carlos Alberto Galeano |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/3300018822284940 |
dc.contributor.author.fl_str_mv |
Nogueira, Danielle Pires |
contributor_str_mv |
Seolatto, Araceli Aparecida Freitas, Fernanda Ferreira Seolatto, Araceli Aparecida Freitas, Fernanda Ferreira Rosa, Paula Rúbia Ferreira Suarez, Carlos Alberto Galeano |
dc.subject.por.fl_str_mv |
Planejamento composto central Xilanase Celulase |
topic |
Planejamento composto central Xilanase Celulase Central composite design Xylanase Cellulase ENGENHARIAS::ENGENHARIA QUIMICA |
dc.subject.eng.fl_str_mv |
Central composite design Xylanase Cellulase |
dc.subject.cnpq.fl_str_mv |
ENGENHARIAS::ENGENHARIA QUIMICA |
description |
Due to the recent research for new fuels from renewable sources ethanol has been gaining momentum, because it can be produced from diverse raw materials, such as agro-industrial residues. The objective with this work was to study the production of reducing sugar by dilute acid, and enzymatic hydrolysis of the orange bagasse from juice industry. The orange bagasse was collected, cut into pieces, and crushed. Granulometry, and the contents of moisture, ashes, holocellulose, cellulose, hemicelulose, soluble and insoluble lignin were determined. The pre-treatment was done with calcium hydroxide following what was previously tested by Silva et al. (2013). A pre-test of the enzymes combination was done using 2 g of pre-treated biomass, in dry base, using 3 FPU/mL of cellulase, and 0 U/g and 3 U/g of xylanase. For the hydrolysis two central composite factorial 2³ designs were done, with the answer in total reducing sugars (ART). For the dilute acid hydrolysis the factors were HCl concentration, temperature, and time, and for the enzymatic the concentrations of cellulase and xylanase and time. The granulometry showed that 47.75% of the biomass with diameter over 0.833 mm, 32.84% of the biomass with an average diameter of 0.564 mm, and 19.41% of the biomass with diameter under 0.295 mm. The moisture content prior to drying was 84.69% and 7.38%, the ashes content was 3.79%. The cellulose content was 22.90% and the hemicellulose was 3.39%. The lignin content was 9.90%. The reducing sugar results for the acid hydrolysis varied from 9.32±0.68 mg ART per g of biomass to 30.15±0.31 mg ART per g biomass, the most significant factor was temperature, and the least was time. It was not possible to find the optimum region with the studied factors. The reducing sugar results for the enzymatic hydrolysis varied from 75.33±3.82 mg ART per g biomass to 99.66±0.62 mg ART per g biomass, the most significant factor was the cellulase concentration, and the least significant the xylanase concentration. The studied factors did not show the optimum region to maximize the reducing sugars content. The enzymatic hydrolysis produced larger concentrations of reducing sugars than the acid hydrolysis. |
publishDate |
2016 |
dc.date.accessioned.fl_str_mv |
2016-09-01T14:42:33Z |
dc.date.issued.fl_str_mv |
2016-04-18 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
NOGUEIRA, D. P. Estudo da obtenção de açúcares redutores totais a partir do bagaço de laranja (Citrus sinenses) por hidrólises ácida diluída e enzimática. 2016. 74 f. Dissertação (Mestrado em Engenharia Química) - Universidade Federal de Goiás, Goiânia, 2016. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/6084 |
identifier_str_mv |
NOGUEIRA, D. P. Estudo da obtenção de açúcares redutores totais a partir do bagaço de laranja (Citrus sinenses) por hidrólises ácida diluída e enzimática. 2016. 74 f. Dissertação (Mestrado em Engenharia Química) - Universidade Federal de Goiás, Goiânia, 2016. |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/6084 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
3481496501158460078 |
dc.relation.confidence.fl_str_mv |
600 600 600 |
dc.relation.department.fl_str_mv |
7826066743741197278 |
dc.relation.cnpq.fl_str_mv |
-1848640261096870878 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.publisher.program.fl_str_mv |
Programa de Pós-graduação em Engenharia Química (IQ) |
dc.publisher.initials.fl_str_mv |
UFG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Instituto de Química - IQ (RG) |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFG instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Repositório Institucional da UFG |
collection |
Repositório Institucional da UFG |
bitstream.url.fl_str_mv |
http://repositorio.bc.ufg.br/tede/bitstreams/3b0e17cf-ee86-4d9f-844a-0d3a43880542/download http://repositorio.bc.ufg.br/tede/bitstreams/c994ba00-08a5-45ba-9da2-2c06f5fc3298/download http://repositorio.bc.ufg.br/tede/bitstreams/7fe1f529-2e52-4fe6-8f2c-0fd5db645d8e/download http://repositorio.bc.ufg.br/tede/bitstreams/114a032d-36c1-476c-b360-bd06b639bfef/download http://repositorio.bc.ufg.br/tede/bitstreams/a16b4c86-7a57-4a0f-974c-d8e14d3ee6d1/download |
bitstream.checksum.fl_str_mv |
bd3efa91386c1718a7f26a329fdcb468 4afdbb8c545fd630ea7db775da747b2f d41d8cd98f00b204e9800998ecf8427e d41d8cd98f00b204e9800998ecf8427e eb50931397d77906f88aaeec4a5fa5d0 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFG - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
tasesdissertacoes.bc@ufg.br |
_version_ |
1798044395788828672 |