Avaliação da susceptibilidade à mastite mediante análise de SNVs do promotor do gene CXCL8 em bovinos de aptidão leiteira

• Autor(a) principal: Campos, Calebe Bertolino Marins de
• Data de Publicação: 2019
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UFG
• Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/9777
• topic: IL8; Bos taurus; Holandesa; QPCR; Holstein; Jersey; CIENCIAS BIOLOGICAS::GENETICA

Agriculture is one of the sectors most responsible for Brazilian GDP, with 7.5% coming from livestock. Given the importance of livestock for the Brazilian economy, it is of great importance to study diseases that affect the national herds and their extrinsic and intrinsic factors. The objective of this study was to verify if there was association of clinical mastitis and subsequently of subclinical mastitis with alleles, genotypes and haplotypes found in six polymorphic sites of the promoter region of the CXCL8 gene. The animals from this study came from two herds of Goiás state, totalizing 98 animals and later the genomic sequences of interest in these animals were identified by qPCR using the KASP® kit (LGC Limited ®). In all the polymorphic sites three different genotypes were found and as in the distribution in the alleles and genotypes, four different haplotypes were identified. The alleles were found to be Hardy-Weinberg equilibrium and the loci were in linkage disequilibrium. The distribution of the animals of the animals in the positive and negative groups for clinical mastitis was different from the distribution found when the somatic cell count (SCC) of the milk was taken into account to separate the animals in the subclinical mastitis groups. No statistically significant associations were found between alleles, genotypes and haplotypes with the occurrence of clinical mastitis. However, there was a significant relationship between the GG genotype of the polymorphic site "KASP 4" (c-687) and the presence of subclinical mastitis, and the haplotype IL8-h2 had a protective effect against subclinical mastitis.