Toxicidade de nanopartículas de óxido de ferro (γ-Fe2O3) funcionalizadas com citrato ao longo do desenvolvimento inicial do zebrafish (Danio rerio)
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/12472 |
Resumo: | Iron oxide nanoparticles (NOFs) are being increasingly used in medical, environmental and technological applications. However, with its growing production and application, concerns arise about its release into the environment and its impact on human and environmental health. In this sense, the zebrafish (Danio rerio) is proving to be an excellent model system for the analysis and classification of nanomaterial toxicity. Thus, the general objective of the present study was to evaluate the toxicity of NOFs (γ-Fe2O3 NPs) functionalized with citrate throughout the initial development of zebrafish, comparing toxicity with iron ions, after static and semi-static exposure. For this purpose, the zebrafish embryos were exposed in static (without medium renewal) and semi-static (medium renewal every 24 h) exposure modes to γ-Fe2O3 NPs functionalized with citrate and iron chloride in different concentrations environmentally relevant iron (0.3, 0.6, 1.25, 2.5, 5.0 and 10 mg L-1) for 144 h, together with the control group maintained in water reconstituted in 24-well plates. The results showed that γ-Fe2O3 NPs were accumulated mainly in the chorion of the embryos and in the digestive system and liver of zebrafish larvae. Γ-Fe2O3 NPs have low toxicity compared to iron ions, indicating that the in vivo toxicity of both forms of iron is mediated by different mechanisms of action. However, NPs caused significant changes after semi-static exposure causing a decrease in heart rate, induced blood accumulation and formation of pericardial edema in zebrafish embryos, indicating its cardiotoxic effect. Regarding the exposure method, it was observed that both forms of iron induced high embryotoxicity under semi-static exposure conditions compared to static exposure, indicating that the toxicity depends on the frequency of exposure. This was the first study on the impact of the exposure condition on the toxicity of γ-Fe2O3 NPs on the development of zebrafish. |
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Rocha, Thiago Lopeshttp://lattes.cnpq.br/6325937100056775Gonçalves, Bruno Bastoshttp://lattes.cnpq.br/3194796958722741Rocha, Thiago LopesRodrigues, Gisele AugustoFranchi, Leonardo Pereirahttp://lattes.cnpq.br/8251665270872856Pereira, Aryelle Canedo2022-12-13T14:03:36Z2022-12-13T14:03:36Z2020-01-24PEREIRA, A. C. Toxicidade de nanopartículas de óxido de ferro (γ-Fe2O3) funcionalizadas com citrato ao longo do desenvolvimento inicial do zebrafish (Danio rerio). 2020. 144 f. Dissertação (Mestrado em Genética e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2020.http://repositorio.bc.ufg.br/tede/handle/tede/12472Iron oxide nanoparticles (NOFs) are being increasingly used in medical, environmental and technological applications. However, with its growing production and application, concerns arise about its release into the environment and its impact on human and environmental health. In this sense, the zebrafish (Danio rerio) is proving to be an excellent model system for the analysis and classification of nanomaterial toxicity. Thus, the general objective of the present study was to evaluate the toxicity of NOFs (γ-Fe2O3 NPs) functionalized with citrate throughout the initial development of zebrafish, comparing toxicity with iron ions, after static and semi-static exposure. For this purpose, the zebrafish embryos were exposed in static (without medium renewal) and semi-static (medium renewal every 24 h) exposure modes to γ-Fe2O3 NPs functionalized with citrate and iron chloride in different concentrations environmentally relevant iron (0.3, 0.6, 1.25, 2.5, 5.0 and 10 mg L-1) for 144 h, together with the control group maintained in water reconstituted in 24-well plates. The results showed that γ-Fe2O3 NPs were accumulated mainly in the chorion of the embryos and in the digestive system and liver of zebrafish larvae. Γ-Fe2O3 NPs have low toxicity compared to iron ions, indicating that the in vivo toxicity of both forms of iron is mediated by different mechanisms of action. However, NPs caused significant changes after semi-static exposure causing a decrease in heart rate, induced blood accumulation and formation of pericardial edema in zebrafish embryos, indicating its cardiotoxic effect. Regarding the exposure method, it was observed that both forms of iron induced high embryotoxicity under semi-static exposure conditions compared to static exposure, indicating that the toxicity depends on the frequency of exposure. This was the first study on the impact of the exposure condition on the toxicity of γ-Fe2O3 NPs on the development of zebrafish.As nanopartículas de óxido de ferro (NOFs) estão sendo cada vez mais utilizadas em aplicações médicas, ambientais e tecnológicas. Contudo, com a sua crescente produção e aplicação surgem preocupações sobre sua liberação no meio ambiente e impacto na saúde humana e ambiental. Nesse sentido, o zebrafish (Danio rerio) veem se mostrando um excelente sistema modelo para análise e classificação da toxicidade dos nanomaterias. Assim, o objetivo geral do presente estudo foi avaliar a toxicidade das NOFs (γ-Fe2O3 NPs) funcionalizadas com citrato ao longo do desenvolvimento inicial do zebrafish, comparando a toxicidade com os íons de ferro, após exposição estática e semi-estática. Para tanto, os embriões de zebrafish foram expostos em modo de exposição estática (sem renovação do meio) e semi-estática (renovação do meio a cada 24 h) às γ-Fe2O3 NPs funcionalizadas com citrato e ao cloreto de ferro em diferentes concentrações ambientalmente relevantes de ferro (0,3, 0,6, 1,25, 2,5, 5,0 e 10 mg L-1) por 144 h, juntamento com o grupo controle mantido em água reconstituída em placas de 24 poços. Os resultados mostraram que as γ-Fe2O3 NPs foram acumuladas principalmente no córion dos embriões e no sistema digestivo e no fígado das larvas de zebrafish. As γ-Fe2O3 NPs apresentam baixa toxicidade em comparação com os íons de ferro, indicando que a toxicidade in vivo de ambas as formas de ferro é mediada por diferentes mecanismos de ação. Entretanto, as NPs causaram alterações significativas após exposição semi-estática causando diminuição da frequência cardíaca, induziram acúmulo de sangue e formação de edema do pericárdio nos embriões do zebrafish, indicando seu efeito cardiotóxico. Em relação ao método de exposição, foi observado que ambas as formas de ferro induziram alta embriotoxicidade em condições de exposição semi-estática em comparação com a exposição estática, indicando que a toxicidade depende da frequência de exposição. Este foi o primeiro estudo sobre o impacto da condição de exposição na toxicidade das γ-Fe2O3 NPs no desenvolvimento do zebrafish.Submitted by Marlene Santos (marlene.bc.ufg@gmail.com) on 2022-12-07T22:38:24Z No. of bitstreams: 2 Dissertação - Aryelle Canedo Pereira - 2020.pdf: 7665502 bytes, checksum: e659a5fd1eefd253aebb4096e8c54ffa (MD5) license_rdf: 805 bytes, checksum: 4460e5956bc1d1639be9ae6146a50347 (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2022-12-13T14:03:36Z (GMT) No. of bitstreams: 2 Dissertação - Aryelle Canedo Pereira - 2020.pdf: 7665502 bytes, checksum: e659a5fd1eefd253aebb4096e8c54ffa (MD5) license_rdf: 805 bytes, checksum: 4460e5956bc1d1639be9ae6146a50347 (MD5)Made available in DSpace on 2022-12-13T14:03:36Z (GMT). No. of bitstreams: 2 Dissertação - Aryelle Canedo Pereira - 2020.pdf: 7665502 bytes, checksum: e659a5fd1eefd253aebb4096e8c54ffa (MD5) license_rdf: 805 bytes, checksum: 4460e5956bc1d1639be9ae6146a50347 (MD5) Previous issue date: 2020-01-24Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporUniversidade Federal de GoiásPrograma de Pós-graduação em Genética e Biologia Molecular (ICB)UFGBrasilInstituto de Ciências Biológicas - ICB (RG)Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessNanoecotoxicologiaNanomateriaisEmbriotoxicidadeCardiotoxicidadeBiomarcadoresNanoecotoxicologyNanomaterialsEmbryoCardiotoxicityBiomarkersCIENCIAS BIOLOGICAS::GENETICA::GENETICA HUMANA E MEDICAToxicidade de nanopartículas de óxido de ferro (γ-Fe2O3) funcionalizadas com citrato ao longo do desenvolvimento inicial do zebrafish (Danio rerio)Toxicity of iron oxide nanoparticles (γ-Fe2O3) functionalized with citrate during the initial development of zebrafish (Danio rerio)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis59500500500500236481reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://repositorio.bc.ufg.br/tede/bitstreams/aefe8ad2-a663-4b58-8021-bae855384a2e/download8a4605be74aa9ea9d79846c1fba20a33MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805http://repositorio.bc.ufg.br/tede/bitstreams/4a99c833-21b4-4577-8f3e-6b852cb9514f/download4460e5956bc1d1639be9ae6146a50347MD52ORIGINALDissertação - Aryelle Canedo Pereira - 2020.pdfDissertação - Aryelle Canedo Pereira - 2020.pdfapplication/pdf7665502http://repositorio.bc.ufg.br/tede/bitstreams/398cd799-56be-4bb6-9924-76642d1b135d/downloade659a5fd1eefd253aebb4096e8c54ffaMD53tede/124722022-12-13 11:03:36.309http://creativecommons.org/licenses/by-nc-nd/4.0/Attribution-NonCommercial-NoDerivatives 4.0 Internationalopen.accessoai:repositorio.bc.ufg.br:tede/12472http://repositorio.bc.ufg.br/tedeRepositório InstitucionalPUBhttp://repositorio.bc.ufg.br/oai/requesttasesdissertacoes.bc@ufg.bropendoar:2022-12-13T14:03:36Repositório Institucional da UFG - Universidade Federal de Goiás (UFG)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 |
dc.title.pt_BR.fl_str_mv |
Toxicidade de nanopartículas de óxido de ferro (γ-Fe2O3) funcionalizadas com citrato ao longo do desenvolvimento inicial do zebrafish (Danio rerio) |
dc.title.alternative.eng.fl_str_mv |
Toxicity of iron oxide nanoparticles (γ-Fe2O3) functionalized with citrate during the initial development of zebrafish (Danio rerio) |
title |
Toxicidade de nanopartículas de óxido de ferro (γ-Fe2O3) funcionalizadas com citrato ao longo do desenvolvimento inicial do zebrafish (Danio rerio) |
spellingShingle |
Toxicidade de nanopartículas de óxido de ferro (γ-Fe2O3) funcionalizadas com citrato ao longo do desenvolvimento inicial do zebrafish (Danio rerio) Pereira, Aryelle Canedo Nanoecotoxicologia Nanomateriais Embriotoxicidade Cardiotoxicidade Biomarcadores Nanoecotoxicology Nanomaterials Embryo Cardiotoxicity Biomarkers CIENCIAS BIOLOGICAS::GENETICA::GENETICA HUMANA E MEDICA |
title_short |
Toxicidade de nanopartículas de óxido de ferro (γ-Fe2O3) funcionalizadas com citrato ao longo do desenvolvimento inicial do zebrafish (Danio rerio) |
title_full |
Toxicidade de nanopartículas de óxido de ferro (γ-Fe2O3) funcionalizadas com citrato ao longo do desenvolvimento inicial do zebrafish (Danio rerio) |
title_fullStr |
Toxicidade de nanopartículas de óxido de ferro (γ-Fe2O3) funcionalizadas com citrato ao longo do desenvolvimento inicial do zebrafish (Danio rerio) |
title_full_unstemmed |
Toxicidade de nanopartículas de óxido de ferro (γ-Fe2O3) funcionalizadas com citrato ao longo do desenvolvimento inicial do zebrafish (Danio rerio) |
title_sort |
Toxicidade de nanopartículas de óxido de ferro (γ-Fe2O3) funcionalizadas com citrato ao longo do desenvolvimento inicial do zebrafish (Danio rerio) |
author |
Pereira, Aryelle Canedo |
author_facet |
Pereira, Aryelle Canedo |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Rocha, Thiago Lopes |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/6325937100056775 |
dc.contributor.advisor-co1.fl_str_mv |
Gonçalves, Bruno Bastos |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/3194796958722741 |
dc.contributor.referee1.fl_str_mv |
Rocha, Thiago Lopes |
dc.contributor.referee2.fl_str_mv |
Rodrigues, Gisele Augusto |
dc.contributor.referee3.fl_str_mv |
Franchi, Leonardo Pereira |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/8251665270872856 |
dc.contributor.author.fl_str_mv |
Pereira, Aryelle Canedo |
contributor_str_mv |
Rocha, Thiago Lopes Gonçalves, Bruno Bastos Rocha, Thiago Lopes Rodrigues, Gisele Augusto Franchi, Leonardo Pereira |
dc.subject.por.fl_str_mv |
Nanoecotoxicologia Nanomateriais Embriotoxicidade Cardiotoxicidade Biomarcadores |
topic |
Nanoecotoxicologia Nanomateriais Embriotoxicidade Cardiotoxicidade Biomarcadores Nanoecotoxicology Nanomaterials Embryo Cardiotoxicity Biomarkers CIENCIAS BIOLOGICAS::GENETICA::GENETICA HUMANA E MEDICA |
dc.subject.eng.fl_str_mv |
Nanoecotoxicology Nanomaterials Embryo Cardiotoxicity Biomarkers |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::GENETICA::GENETICA HUMANA E MEDICA |
description |
Iron oxide nanoparticles (NOFs) are being increasingly used in medical, environmental and technological applications. However, with its growing production and application, concerns arise about its release into the environment and its impact on human and environmental health. In this sense, the zebrafish (Danio rerio) is proving to be an excellent model system for the analysis and classification of nanomaterial toxicity. Thus, the general objective of the present study was to evaluate the toxicity of NOFs (γ-Fe2O3 NPs) functionalized with citrate throughout the initial development of zebrafish, comparing toxicity with iron ions, after static and semi-static exposure. For this purpose, the zebrafish embryos were exposed in static (without medium renewal) and semi-static (medium renewal every 24 h) exposure modes to γ-Fe2O3 NPs functionalized with citrate and iron chloride in different concentrations environmentally relevant iron (0.3, 0.6, 1.25, 2.5, 5.0 and 10 mg L-1) for 144 h, together with the control group maintained in water reconstituted in 24-well plates. The results showed that γ-Fe2O3 NPs were accumulated mainly in the chorion of the embryos and in the digestive system and liver of zebrafish larvae. Γ-Fe2O3 NPs have low toxicity compared to iron ions, indicating that the in vivo toxicity of both forms of iron is mediated by different mechanisms of action. However, NPs caused significant changes after semi-static exposure causing a decrease in heart rate, induced blood accumulation and formation of pericardial edema in zebrafish embryos, indicating its cardiotoxic effect. Regarding the exposure method, it was observed that both forms of iron induced high embryotoxicity under semi-static exposure conditions compared to static exposure, indicating that the toxicity depends on the frequency of exposure. This was the first study on the impact of the exposure condition on the toxicity of γ-Fe2O3 NPs on the development of zebrafish. |
publishDate |
2020 |
dc.date.issued.fl_str_mv |
2020-01-24 |
dc.date.accessioned.fl_str_mv |
2022-12-13T14:03:36Z |
dc.date.available.fl_str_mv |
2022-12-13T14:03:36Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
PEREIRA, A. C. Toxicidade de nanopartículas de óxido de ferro (γ-Fe2O3) funcionalizadas com citrato ao longo do desenvolvimento inicial do zebrafish (Danio rerio). 2020. 144 f. Dissertação (Mestrado em Genética e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2020. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/12472 |
identifier_str_mv |
PEREIRA, A. C. Toxicidade de nanopartículas de óxido de ferro (γ-Fe2O3) funcionalizadas com citrato ao longo do desenvolvimento inicial do zebrafish (Danio rerio). 2020. 144 f. Dissertação (Mestrado em Genética e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2020. |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/12472 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
59 |
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500 500 500 500 |
dc.relation.department.fl_str_mv |
23 |
dc.relation.cnpq.fl_str_mv |
648 |
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1 |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.publisher.program.fl_str_mv |
Programa de Pós-graduação em Genética e Biologia Molecular (ICB) |
dc.publisher.initials.fl_str_mv |
UFG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Instituto de Ciências Biológicas - ICB (RG) |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFG instname:Universidade Federal de Goiás (UFG) instacron:UFG |
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Universidade Federal de Goiás (UFG) |
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UFG |
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UFG |
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Repositório Institucional da UFG |
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