O polimorfismo do gene p5372(RP) no câncer de cabeça e pescoço: estudo de associação e meta-análise
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tde/1012 |
Resumo: | Head and neck cancer arises in the oral cavity and nearby regions, larynx, pharynx, including oropharynx, nasopharynx, and hipopharynx. Extensive epidemiologic studies have revealed that chronic tobacco smoking and alcohol consumption as the two main risk factors associated with the multifactorial etiology of head and neck cancers. Additionally, nutritional status, HPV infection, and genetic polymorphism were also related with the disease. A frequently studied polymorphism in Squamous Cell Carcinoma (SCC) of head and neck is a G-to-C SNP (Single Nucleotide Polymorphism) at codon 72 in the p53 gene, which codes for an Arg or Pro (Arginine or Proline) in P53 protein. In order to investigate the distribution and potential association of that SNP in SCC, biological samples were obtained from 331 cases of head and neck SCC from Araújo Jorge Hospital and 271 healthy control individuals from Goiânia (Brazil) population. DNA was isolated and subsequently used for PCR amplification to genotype cases and controls with respect to their p5372 SNP. Additionaly, a meta-analysis was carried out using 29 relevant case-control studies that used p5372 SNP genotyping in SCC of the head and neck. Allelic frequencies for cases were 73.3% and 27.7% for Arg and Pro, respectively. On the other hand, control allelic frequencies were 74.2% and 25.8% for Arg and Pro, respectively (p = 0.119). Genotypic frequencies were 56.8% Arg/Arg, 32.9% Arg/Pro, and 10.3% Pro/Pro for all cases. The control genotypic frequencies were 61.3% Arg/Arg, 25.8% Arg/Pro and 12.9% Pro/Pro (p = 0,137). According to the current data and meta-analysis, no association between p5372 SNP and the development of SCC of the head and neck was found. Although, the homozygous genotype Arg/Arg was found as an important oncogenic risk factor associated with the carcinoma of the oropharynx. |
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CRUZ, Aparecido Divino dahttp://lattes.cnpq.br/7868817504129985http://lattes.cnpq.br/4256300529988960SILVA, Antonio Márcio Teodoro Cordeiro2014-07-29T15:10:31Z2010-11-032009-10-20SILVA, Antonio Márcio Teodoro Cordeiro. p5372(RP) polymorphism in head and neck cancer: an association and meta-analysis study. 2009. 89 f. Tese (Doutorado em Ciencias Biologicas) - Universidade Federal de Goiás, Goiânia, 2009.http://repositorio.bc.ufg.br/tede/handle/tde/1012Head and neck cancer arises in the oral cavity and nearby regions, larynx, pharynx, including oropharynx, nasopharynx, and hipopharynx. Extensive epidemiologic studies have revealed that chronic tobacco smoking and alcohol consumption as the two main risk factors associated with the multifactorial etiology of head and neck cancers. Additionally, nutritional status, HPV infection, and genetic polymorphism were also related with the disease. A frequently studied polymorphism in Squamous Cell Carcinoma (SCC) of head and neck is a G-to-C SNP (Single Nucleotide Polymorphism) at codon 72 in the p53 gene, which codes for an Arg or Pro (Arginine or Proline) in P53 protein. In order to investigate the distribution and potential association of that SNP in SCC, biological samples were obtained from 331 cases of head and neck SCC from Araújo Jorge Hospital and 271 healthy control individuals from Goiânia (Brazil) population. DNA was isolated and subsequently used for PCR amplification to genotype cases and controls with respect to their p5372 SNP. Additionaly, a meta-analysis was carried out using 29 relevant case-control studies that used p5372 SNP genotyping in SCC of the head and neck. Allelic frequencies for cases were 73.3% and 27.7% for Arg and Pro, respectively. On the other hand, control allelic frequencies were 74.2% and 25.8% for Arg and Pro, respectively (p = 0.119). Genotypic frequencies were 56.8% Arg/Arg, 32.9% Arg/Pro, and 10.3% Pro/Pro for all cases. The control genotypic frequencies were 61.3% Arg/Arg, 25.8% Arg/Pro and 12.9% Pro/Pro (p = 0,137). According to the current data and meta-analysis, no association between p5372 SNP and the development of SCC of the head and neck was found. Although, the homozygous genotype Arg/Arg was found as an important oncogenic risk factor associated with the carcinoma of the oropharynx.O câncer de cabeça e pescoço ocorre nas regiões da cavidade oral, da faringe (orofaringe, nasofaringe e hipofaringe) e da laringe. Aspectos epidemiológicos evidenciam a etiologia multifatorial das neoplasias de cabeça e pescoço, destacando, o tabagismo e o etilismo crônicos como principais fatores de risco, seguidos dos aspectos nutricionais, infecções pelo HPV e polimorfismos genéticos. No contexto dos polimorfismos genéticos tem-se o gene p5372(RP). O objetivo do presente estudo foi estabelecer a associação entre o polimorfismo do gene p53, no códon 72 (Arginina/Prolina), e o câncer de cabeça e pescoço. O estudo contou com 602 participantes, divididos em: 331 pacientes com câncer de cabeça e pescoço provenientes do Hospital Araújo Jorge (grupo caso) e 271 indivíduos saudáveis selecionados randomicamente na população da cidade de Goiânia/GO (grupo controle). As amostras foram processadas e o DNA foi isolado para a avaliação molecular, por PCR, do polimorfismo do gene p5372(RP). Realizou-se também uma meta-análise com 29 estudos do tipo caso-controle de avaliação do referido polimorfismo em câncer de cabeça e pescoço. As frequências alélicas, dos 602 participantes para p53Arg e p53Pro, foram, respectivamente, 73,3% e 26,7% no grupo caso e 74,2% e 25,8% no grupo controle (p = 0,119). As frequências genotípicas foram, para o grupo caso, 56,8% Arg/Arg, 32,9% Arg/Pro e 10,3% Pro/Pro; e para o grupo controle, 61,3% Arg/Arg, 25,8% Arg/Pro e 12,9% Pro/Pro (p = 0,137). A meta-análise agrupou 29 estudos sobre o polimorfismo de p5372(RP) em câncer de cabeça e pescoço e não indicou nenhum tipo de correlação entre as variáveis consideradas. Os dados da presente avaliação e da meta-análise sugerem ausência de correlação entre o polimorfismo do gene p5372(RP) e o risco de desenvolver o câncer de cabeça e pescoço. Por outro lado, ao estratificar os dados por sítio anatômico, o genótipo Arg/Arg se revelou como um importante fator de risco oncogênico para o desenvolvimento do câncer da orofaringe quando comparado com os outros genótipos.Made available in DSpace on 2014-07-29T15:10:31Z (GMT). No. of bitstreams: 1 antonio marcio.pdf: 1664898 bytes, checksum: a51767c9c1070efdc7346160e72afa12 (MD5) Previous issue date: 2009-10-20application/pdfhttp://repositorio.bc.ufg.br/TEDE/retrieve/3571/antonio%20marcio.pdf.jpgporUniversidade Federal de GoiásDoutorado em BiologiaUFGBRCiencias Biologicasp53polimorfismocâncercabeçapescoçooralfaringelaringemeta-análisep53, polymorphism, cancer, head and neck, oral, pharynx, larynx, meta-analysisCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULARO polimorfismo do gene p5372(RP) no câncer de cabeça e pescoço: estudo de associação e meta-análisep5372(RP) polymorphism in head and neck cancer: an association and meta-analysis studyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGORIGINALantonio marcio.pdfapplication/pdf1664898http://repositorio.bc.ufg.br/tede/bitstreams/37c71366-bb63-4dd1-b3b4-466e615d6040/downloada51767c9c1070efdc7346160e72afa12MD51TEXTantonio marcio.pdf.txtantonio marcio.pdf.txtExtracted Texttext/plain163419http://repositorio.bc.ufg.br/tede/bitstreams/b363c56c-399c-4dac-92e5-55fa3e6e6007/download821500cf12a0915ebf669961056ceffdMD52THUMBNAILantonio marcio.pdf.jpgantonio marcio.pdf.jpgGenerated Thumbnailimage/jpeg2998http://repositorio.bc.ufg.br/tede/bitstreams/270067aa-f723-4822-9593-1fed0714ef6b/download25570e430cefbea03c5ef9a5d7ba0712MD53tde/10122014-07-30 03:06:34.34open.accessoai:repositorio.bc.ufg.br:tde/1012http://repositorio.bc.ufg.br/tedeRepositório InstitucionalPUBhttp://repositorio.bc.ufg.br/oai/requesttasesdissertacoes.bc@ufg.bropendoar:2014-07-30T06:06:34Repositório Institucional da UFG - Universidade Federal de Goiás (UFG)false |
dc.title.por.fl_str_mv |
O polimorfismo do gene p5372(RP) no câncer de cabeça e pescoço: estudo de associação e meta-análise |
dc.title.alternative.eng.fl_str_mv |
p5372(RP) polymorphism in head and neck cancer: an association and meta-analysis study |
title |
O polimorfismo do gene p5372(RP) no câncer de cabeça e pescoço: estudo de associação e meta-análise |
spellingShingle |
O polimorfismo do gene p5372(RP) no câncer de cabeça e pescoço: estudo de associação e meta-análise SILVA, Antonio Márcio Teodoro Cordeiro p53 polimorfismo câncer cabeça pescoço oral faringe laringe meta-análise p53, polymorphism, cancer, head and neck, oral, pharynx, larynx, meta-analysis CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULAR |
title_short |
O polimorfismo do gene p5372(RP) no câncer de cabeça e pescoço: estudo de associação e meta-análise |
title_full |
O polimorfismo do gene p5372(RP) no câncer de cabeça e pescoço: estudo de associação e meta-análise |
title_fullStr |
O polimorfismo do gene p5372(RP) no câncer de cabeça e pescoço: estudo de associação e meta-análise |
title_full_unstemmed |
O polimorfismo do gene p5372(RP) no câncer de cabeça e pescoço: estudo de associação e meta-análise |
title_sort |
O polimorfismo do gene p5372(RP) no câncer de cabeça e pescoço: estudo de associação e meta-análise |
author |
SILVA, Antonio Márcio Teodoro Cordeiro |
author_facet |
SILVA, Antonio Márcio Teodoro Cordeiro |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
CRUZ, Aparecido Divino da |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/7868817504129985 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/4256300529988960 |
dc.contributor.author.fl_str_mv |
SILVA, Antonio Márcio Teodoro Cordeiro |
contributor_str_mv |
CRUZ, Aparecido Divino da |
dc.subject.por.fl_str_mv |
p53 polimorfismo câncer cabeça pescoço oral faringe laringe meta-análise |
topic |
p53 polimorfismo câncer cabeça pescoço oral faringe laringe meta-análise p53, polymorphism, cancer, head and neck, oral, pharynx, larynx, meta-analysis CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULAR |
dc.subject.eng.fl_str_mv |
p53, polymorphism, cancer, head and neck, oral, pharynx, larynx, meta-analysis |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULAR |
description |
Head and neck cancer arises in the oral cavity and nearby regions, larynx, pharynx, including oropharynx, nasopharynx, and hipopharynx. Extensive epidemiologic studies have revealed that chronic tobacco smoking and alcohol consumption as the two main risk factors associated with the multifactorial etiology of head and neck cancers. Additionally, nutritional status, HPV infection, and genetic polymorphism were also related with the disease. A frequently studied polymorphism in Squamous Cell Carcinoma (SCC) of head and neck is a G-to-C SNP (Single Nucleotide Polymorphism) at codon 72 in the p53 gene, which codes for an Arg or Pro (Arginine or Proline) in P53 protein. In order to investigate the distribution and potential association of that SNP in SCC, biological samples were obtained from 331 cases of head and neck SCC from Araújo Jorge Hospital and 271 healthy control individuals from Goiânia (Brazil) population. DNA was isolated and subsequently used for PCR amplification to genotype cases and controls with respect to their p5372 SNP. Additionaly, a meta-analysis was carried out using 29 relevant case-control studies that used p5372 SNP genotyping in SCC of the head and neck. Allelic frequencies for cases were 73.3% and 27.7% for Arg and Pro, respectively. On the other hand, control allelic frequencies were 74.2% and 25.8% for Arg and Pro, respectively (p = 0.119). Genotypic frequencies were 56.8% Arg/Arg, 32.9% Arg/Pro, and 10.3% Pro/Pro for all cases. The control genotypic frequencies were 61.3% Arg/Arg, 25.8% Arg/Pro and 12.9% Pro/Pro (p = 0,137). According to the current data and meta-analysis, no association between p5372 SNP and the development of SCC of the head and neck was found. Although, the homozygous genotype Arg/Arg was found as an important oncogenic risk factor associated with the carcinoma of the oropharynx. |
publishDate |
2009 |
dc.date.issued.fl_str_mv |
2009-10-20 |
dc.date.available.fl_str_mv |
2010-11-03 |
dc.date.accessioned.fl_str_mv |
2014-07-29T15:10:31Z |
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dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
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doctoralThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
SILVA, Antonio Márcio Teodoro Cordeiro. p5372(RP) polymorphism in head and neck cancer: an association and meta-analysis study. 2009. 89 f. Tese (Doutorado em Ciencias Biologicas) - Universidade Federal de Goiás, Goiânia, 2009. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tde/1012 |
identifier_str_mv |
SILVA, Antonio Márcio Teodoro Cordeiro. p5372(RP) polymorphism in head and neck cancer: an association and meta-analysis study. 2009. 89 f. Tese (Doutorado em Ciencias Biologicas) - Universidade Federal de Goiás, Goiânia, 2009. |
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http://repositorio.bc.ufg.br/tede/handle/tde/1012 |
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UFG |
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Universidade Federal de Goiás |
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