Suppression of Fusarium wilt in tomato plants by rhizobacteria from the Bacillus genus
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Pesquisa Agropecuária Tropical (Online) |
Texto Completo: | https://revistas.ufg.br/pat/article/view/35397 |
Resumo: | Vascular wilting in tomato plants constitutes an important group of diseases incited by soil inhabiting pathogens, especially Fusarium oxysporum f. sp. lycopersici, that causes significant production losses. The introgression of genes that express specific resistance is considered the main control method, however, due to the rapid emergence of races that suppress resistance, biocontrol has been presented as an option to be integrated with genetic control methods. This study aimed at evaluating the ability of rhizobacteria isolates, especially from the Bacillus genus, as biological control agents of Fusarium wilt in tomato plants. The laboratory assay was carried out to verify the ability of the isolates in acting as inhibitors of mycelial growth, besides detecting the genes involved in the expression of polypeptides with antimicrobial action. In the greenhouse, the assay comprised of microbiolized seeds and addition of a suspension of propagules of each rhizobacteria to the soil at the moment of transplanting the tomato seedlings. All the rhizobateria isolates showed variable control levels of the pathogen in vitro. The UFG-07 and UFG-10 isolates, with high similarity with Bacillus subtilis and Bacillus circulans, excelled in the disease suppression. PCR results detected target genes for expression of polypeptides, what reinforces the hypothesis of action of antimicrobial substances in biocontrol. |
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Suppression of Fusarium wilt in tomato plants by rhizobacteria from the Bacillus genusSupressão da murcha de fusário em tomateiro por rizobactérias do gênero BacillusFusarium oxysporumSolanaceaesecondary metabolites.Fusarium oxysporumSolanaceaemetabólitos secundários.Vascular wilting in tomato plants constitutes an important group of diseases incited by soil inhabiting pathogens, especially Fusarium oxysporum f. sp. lycopersici, that causes significant production losses. The introgression of genes that express specific resistance is considered the main control method, however, due to the rapid emergence of races that suppress resistance, biocontrol has been presented as an option to be integrated with genetic control methods. This study aimed at evaluating the ability of rhizobacteria isolates, especially from the Bacillus genus, as biological control agents of Fusarium wilt in tomato plants. The laboratory assay was carried out to verify the ability of the isolates in acting as inhibitors of mycelial growth, besides detecting the genes involved in the expression of polypeptides with antimicrobial action. In the greenhouse, the assay comprised of microbiolized seeds and addition of a suspension of propagules of each rhizobacteria to the soil at the moment of transplanting the tomato seedlings. All the rhizobateria isolates showed variable control levels of the pathogen in vitro. The UFG-07 and UFG-10 isolates, with high similarity with Bacillus subtilis and Bacillus circulans, excelled in the disease suppression. PCR results detected target genes for expression of polypeptides, what reinforces the hypothesis of action of antimicrobial substances in biocontrol.Murchas vasculares em tomateiro constituem importante grupo de doenças incitadas por patógenos habitantes de solos, com destaque para Fusarium oxysporum f. sp. lycopersici, que ocasiona perdas significativas de produção. A introgressão de genes que expressam resistência é a principal medida de controle, mas, devido à rápida emergência de raças que suplantam a resistência, vem aumentando a importância do biocontrole, em integração com o controle genético. Objetivou-se avaliar a capacidade de isolados de rizobactérias, especialmente do gênero Bacillus, como agentes de biocontrole da murcha de fusário em tomateiro. O ensaio de laboratório consistiu na verificação da habilidade de os isolados atuarem como inibidores de crescimento micelial, além da detecção de genes envolvidos na expressão de polipeptídios de ação antimicrobiana. Em casa-de-vegetação, o ensaio foi composto por sementes microbiolizadas e adição, ao solo, de suspensão de propágulos de cada rizobactéria, no momento do transplantio das mudas de tomateiro. Todos os isolados de rizobactérias apresentaram controle variável do patógeno in vitro. Os isolados UFG-07 e UFG-10, com alta similaridade a Bacillus subtilis e Bacillus circulans, destacaram-se na supressão da doença. Resultados da PCR detectaram genes-alvo para expressão de polipeptídios, o que reforça a hipótese da ação de substâncias antimicrobianas no biocontrole.Escola de Agronomia - Universidade Federal de Goiás2015-09-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistas.ufg.br/pat/article/view/35397Pesquisa Agropecuária Tropical [Agricultural Research in the Tropics]; v. 45, n. 3, Jul./Sep. 2015; 356-363Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics); v. 45, n. 3, jul./set. 2015; 356-363Pesquisa Agropecuária Tropical; v. 45, n. 3, jul./set. 2015; 356-3631983-4063reponame:Pesquisa Agropecuária Tropical (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/pat/article/view/35397/19024Copyright (c) 2020 Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics)info:eu-repo/semantics/openAccessCarrer Filho, RenatoDianese, Érico de CamposCunha, Marcos Gomes da2020-07-13T16:30:28Zoai:ojs.revistas.ufg.br:article/35397Revistahttps://revistas.ufg.br/patPUBhttps://revistas.ufg.br/pat/oaiaseleguini.pat@gmail.com||mgoes@agro.ufg.br1983-40631517-6398opendoar:2024-05-21T19:56:08.979471Pesquisa Agropecuária Tropical (Online) - Universidade Federal de Goiás (UFG)true |
dc.title.none.fl_str_mv |
Suppression of Fusarium wilt in tomato plants by rhizobacteria from the Bacillus genus Supressão da murcha de fusário em tomateiro por rizobactérias do gênero Bacillus |
title |
Suppression of Fusarium wilt in tomato plants by rhizobacteria from the Bacillus genus |
spellingShingle |
Suppression of Fusarium wilt in tomato plants by rhizobacteria from the Bacillus genus Carrer Filho, Renato Fusarium oxysporum Solanaceae secondary metabolites. Fusarium oxysporum Solanaceae metabólitos secundários. |
title_short |
Suppression of Fusarium wilt in tomato plants by rhizobacteria from the Bacillus genus |
title_full |
Suppression of Fusarium wilt in tomato plants by rhizobacteria from the Bacillus genus |
title_fullStr |
Suppression of Fusarium wilt in tomato plants by rhizobacteria from the Bacillus genus |
title_full_unstemmed |
Suppression of Fusarium wilt in tomato plants by rhizobacteria from the Bacillus genus |
title_sort |
Suppression of Fusarium wilt in tomato plants by rhizobacteria from the Bacillus genus |
author |
Carrer Filho, Renato |
author_facet |
Carrer Filho, Renato Dianese, Érico de Campos Cunha, Marcos Gomes da |
author_role |
author |
author2 |
Dianese, Érico de Campos Cunha, Marcos Gomes da |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Carrer Filho, Renato Dianese, Érico de Campos Cunha, Marcos Gomes da |
dc.subject.por.fl_str_mv |
Fusarium oxysporum Solanaceae secondary metabolites. Fusarium oxysporum Solanaceae metabólitos secundários. |
topic |
Fusarium oxysporum Solanaceae secondary metabolites. Fusarium oxysporum Solanaceae metabólitos secundários. |
description |
Vascular wilting in tomato plants constitutes an important group of diseases incited by soil inhabiting pathogens, especially Fusarium oxysporum f. sp. lycopersici, that causes significant production losses. The introgression of genes that express specific resistance is considered the main control method, however, due to the rapid emergence of races that suppress resistance, biocontrol has been presented as an option to be integrated with genetic control methods. This study aimed at evaluating the ability of rhizobacteria isolates, especially from the Bacillus genus, as biological control agents of Fusarium wilt in tomato plants. The laboratory assay was carried out to verify the ability of the isolates in acting as inhibitors of mycelial growth, besides detecting the genes involved in the expression of polypeptides with antimicrobial action. In the greenhouse, the assay comprised of microbiolized seeds and addition of a suspension of propagules of each rhizobacteria to the soil at the moment of transplanting the tomato seedlings. All the rhizobateria isolates showed variable control levels of the pathogen in vitro. The UFG-07 and UFG-10 isolates, with high similarity with Bacillus subtilis and Bacillus circulans, excelled in the disease suppression. PCR results detected target genes for expression of polypeptides, what reinforces the hypothesis of action of antimicrobial substances in biocontrol. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-09-29 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistas.ufg.br/pat/article/view/35397 |
url |
https://revistas.ufg.br/pat/article/view/35397 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://revistas.ufg.br/pat/article/view/35397/19024 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2020 Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics) info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2020 Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics) |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Escola de Agronomia - Universidade Federal de Goiás |
publisher.none.fl_str_mv |
Escola de Agronomia - Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
Pesquisa Agropecuária Tropical [Agricultural Research in the Tropics]; v. 45, n. 3, Jul./Sep. 2015; 356-363 Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics); v. 45, n. 3, jul./set. 2015; 356-363 Pesquisa Agropecuária Tropical; v. 45, n. 3, jul./set. 2015; 356-363 1983-4063 reponame:Pesquisa Agropecuária Tropical (Online) instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Pesquisa Agropecuária Tropical (Online) |
collection |
Pesquisa Agropecuária Tropical (Online) |
repository.name.fl_str_mv |
Pesquisa Agropecuária Tropical (Online) - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
aseleguini.pat@gmail.com||mgoes@agro.ufg.br |
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1799874818948988928 |