EFFECT OF FIBROLITIC ENZYMES ON RUMEN MICROBIAL DEGRADATION OF SUGARCANE FIBER
Autor(a) principal: | |
---|---|
Data de Publicação: | 2010 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Ciência animal brasileira (Online) |
Texto Completo: | https://revistas.ufg.br/vet/article/view/4796 |
Resumo: | Aiming to study the limiting factors for degradation of sugarcane fiber to be used as cattle feed, sugarcane fibrous components were fractioned in neutral- and acid-detergent fibers (respectively, NDF and ADF). Whole sugarcane and its fibers were incubated with rumen bacteria, in presence or absence of fibrolytic enzymes (Fibrozyme, Alltech Inc.). Microbial growth and total bacteria count were determined, and the growth rate, generation rate, lag time, and degradability were also determined. Results were analyzed in randomized block design, with a 2x3 factorial arrangement, with two doses of enzymes, and three substrates, using four replications for incubation. The highest microbial growth was observed for whole sugarcane, and the lowest for its fibers. The in vitro degradability was 0.701, 0.392 and 0.191, respectively for whole sugarcane, NDF and ADF (P<0.01). ADF fraction with the addition of fibrolytic enzyme had its degradability increased from 0.387 to 0.425 (P<0.01). Microbial growth was limited in fibrous fractions, possibly due to lignification grade of cell walls. The highest in vitro degradability of fibrous fractions is related to the presence of soluble sugars. Addition of fibrolytic enzymes increased the maximum microbial growth and ADF degradability, indicating that it could be a potential addictive to enhance diets containing sugarcane. KEYWORDS: Cell wall, in vitro degradability, microbial growth. |
id |
UFG-7_2d14b723d7a996bf3201f8b0209763c9 |
---|---|
oai_identifier_str |
oai:ojs.revistas.ufg.br:article/4796 |
network_acronym_str |
UFG-7 |
network_name_str |
Ciência animal brasileira (Online) |
repository_id_str |
|
spelling |
EFFECT OF FIBROLITIC ENZYMES ON RUMEN MICROBIAL DEGRADATION OF SUGARCANE FIBEREFEITO DE ENZIMAS FIBROLÍTICAS SOBRE A DEGRADAÇÃO MICROBIANA RUMINAL DA FIBRA DE CANA-DE-AÇÚCARCattle productioncrescimento microbianoparede celulardegradabilidade in vitroAiming to study the limiting factors for degradation of sugarcane fiber to be used as cattle feed, sugarcane fibrous components were fractioned in neutral- and acid-detergent fibers (respectively, NDF and ADF). Whole sugarcane and its fibers were incubated with rumen bacteria, in presence or absence of fibrolytic enzymes (Fibrozyme, Alltech Inc.). Microbial growth and total bacteria count were determined, and the growth rate, generation rate, lag time, and degradability were also determined. Results were analyzed in randomized block design, with a 2x3 factorial arrangement, with two doses of enzymes, and three substrates, using four replications for incubation. The highest microbial growth was observed for whole sugarcane, and the lowest for its fibers. The in vitro degradability was 0.701, 0.392 and 0.191, respectively for whole sugarcane, NDF and ADF (P<0.01). ADF fraction with the addition of fibrolytic enzyme had its degradability increased from 0.387 to 0.425 (P<0.01). Microbial growth was limited in fibrous fractions, possibly due to lignification grade of cell walls. The highest in vitro degradability of fibrous fractions is related to the presence of soluble sugars. Addition of fibrolytic enzymes increased the maximum microbial growth and ADF degradability, indicating that it could be a potential addictive to enhance diets containing sugarcane. KEYWORDS: Cell wall, in vitro degradability, microbial growth.Com o objetivo de estudar os limitantes da degradação da fibra da cana-de-açúcar para utilizá-la como alimento para bovinos, fracionaram-se os componentes fibrosos da cana-de-açúcar (variedade Mex69-290) em fibra em detergente neutro (FDN) e em fibra em detergente ácido (FDA). A cana integral e as fibras foram incubadas com bactérias ruminais, na presença ou ausência de enzimas fibrolíticas (Fibrozyme, Alltech Inc.). Determinaram-se o crescimento microbiano e as bactérias totais, assim como a taxa de crescimento, a taxa de geração, o tempo de colonização e a degradabilidade. Os resultados foram analisados em um delinea­mento de blocos ao acaso, com arranjo fatorial 2 x 3, com duas doses de enzima e três substratos, utilizando quatro repetições de incubação. Observou-se o maior crescimento microbiano para cana integral e os menores para suas fibras. A degradabilidade in vitro foi de 0,701, 0,392 e 0,191, respectivamente para cana integral, FDN e FDA (P<0,01). A fração FDA com a adição das enzimas fibrolíticas teve sua degradabilidade aumentada de 0,387 para 0,425 (P<0,01). O crescimento microbiano foi limitado nas frações fibrosas, possivelmente pelo grau de lignificação das paredes celulares. A maior degradabilidade in vitro das frações fibrosas está associada à presença de açúcares solúveis. A adição de enzimas fibrolíticas aumentou o crescimento microbiano máximo e a digestibilidade da FDA, indicando que estas podem ser um potencial aditivo para melhorar o aproveitamento de dietas com cana-de-açúcar.PALAVRAS-CHAVES: Crescimento microbiano, degradabilidade in vitro, parede celular.Universidade Federal de Goiás2010-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionResearchAvaliado por paresPesquisa científicaapplication/pdfapplication/pdftext/htmlhttps://revistas.ufg.br/vet/article/view/479610.5216/cab.v11i3.4796Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 11 No. 3 (2010); 488 - 491Ciência Animal Brasileira / Brazilian Animal Science; v. 11 n. 3 (2010); 488 - 4911809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/4796/7906https://revistas.ufg.br/vet/article/view/4796/8593https://revistas.ufg.br/vet/article/view/4796/8600Aranda Ibáñez, Emilio ManuelMendoza Martínez, Germán DavidRamos Juárez, Jesús AlbertoBueno, Ives Cláudio da SilvaVitti, André Césarinfo:eu-repo/semantics/openAccess2010-10-02T15:22:45Zoai:ojs.revistas.ufg.br:article/4796Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:55:17.351016Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true |
dc.title.none.fl_str_mv |
EFFECT OF FIBROLITIC ENZYMES ON RUMEN MICROBIAL DEGRADATION OF SUGARCANE FIBER EFEITO DE ENZIMAS FIBROLÍTICAS SOBRE A DEGRADAÇÃO MICROBIANA RUMINAL DA FIBRA DE CANA-DE-AÇÚCAR |
title |
EFFECT OF FIBROLITIC ENZYMES ON RUMEN MICROBIAL DEGRADATION OF SUGARCANE FIBER |
spellingShingle |
EFFECT OF FIBROLITIC ENZYMES ON RUMEN MICROBIAL DEGRADATION OF SUGARCANE FIBER Aranda Ibáñez, Emilio Manuel Cattle production crescimento microbiano parede celular degradabilidade in vitro |
title_short |
EFFECT OF FIBROLITIC ENZYMES ON RUMEN MICROBIAL DEGRADATION OF SUGARCANE FIBER |
title_full |
EFFECT OF FIBROLITIC ENZYMES ON RUMEN MICROBIAL DEGRADATION OF SUGARCANE FIBER |
title_fullStr |
EFFECT OF FIBROLITIC ENZYMES ON RUMEN MICROBIAL DEGRADATION OF SUGARCANE FIBER |
title_full_unstemmed |
EFFECT OF FIBROLITIC ENZYMES ON RUMEN MICROBIAL DEGRADATION OF SUGARCANE FIBER |
title_sort |
EFFECT OF FIBROLITIC ENZYMES ON RUMEN MICROBIAL DEGRADATION OF SUGARCANE FIBER |
author |
Aranda Ibáñez, Emilio Manuel |
author_facet |
Aranda Ibáñez, Emilio Manuel Mendoza Martínez, Germán David Ramos Juárez, Jesús Alberto Bueno, Ives Cláudio da Silva Vitti, André César |
author_role |
author |
author2 |
Mendoza Martínez, Germán David Ramos Juárez, Jesús Alberto Bueno, Ives Cláudio da Silva Vitti, André César |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Aranda Ibáñez, Emilio Manuel Mendoza Martínez, Germán David Ramos Juárez, Jesús Alberto Bueno, Ives Cláudio da Silva Vitti, André César |
dc.subject.por.fl_str_mv |
Cattle production crescimento microbiano parede celular degradabilidade in vitro |
topic |
Cattle production crescimento microbiano parede celular degradabilidade in vitro |
description |
Aiming to study the limiting factors for degradation of sugarcane fiber to be used as cattle feed, sugarcane fibrous components were fractioned in neutral- and acid-detergent fibers (respectively, NDF and ADF). Whole sugarcane and its fibers were incubated with rumen bacteria, in presence or absence of fibrolytic enzymes (Fibrozyme, Alltech Inc.). Microbial growth and total bacteria count were determined, and the growth rate, generation rate, lag time, and degradability were also determined. Results were analyzed in randomized block design, with a 2x3 factorial arrangement, with two doses of enzymes, and three substrates, using four replications for incubation. The highest microbial growth was observed for whole sugarcane, and the lowest for its fibers. The in vitro degradability was 0.701, 0.392 and 0.191, respectively for whole sugarcane, NDF and ADF (P<0.01). ADF fraction with the addition of fibrolytic enzyme had its degradability increased from 0.387 to 0.425 (P<0.01). Microbial growth was limited in fibrous fractions, possibly due to lignification grade of cell walls. The highest in vitro degradability of fibrous fractions is related to the presence of soluble sugars. Addition of fibrolytic enzymes increased the maximum microbial growth and ADF degradability, indicating that it could be a potential addictive to enhance diets containing sugarcane. KEYWORDS: Cell wall, in vitro degradability, microbial growth. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Research Avaliado por pares Pesquisa científica |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistas.ufg.br/vet/article/view/4796 10.5216/cab.v11i3.4796 |
url |
https://revistas.ufg.br/vet/article/view/4796 |
identifier_str_mv |
10.5216/cab.v11i3.4796 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://revistas.ufg.br/vet/article/view/4796/7906 https://revistas.ufg.br/vet/article/view/4796/8593 https://revistas.ufg.br/vet/article/view/4796/8600 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 11 No. 3 (2010); 488 - 491 Ciência Animal Brasileira / Brazilian Animal Science; v. 11 n. 3 (2010); 488 - 491 1809-6891 1518-2797 reponame:Ciência animal brasileira (Online) instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Ciência animal brasileira (Online) |
collection |
Ciência animal brasileira (Online) |
repository.name.fl_str_mv |
Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
||revistacab@gmail.com |
_version_ |
1799874785096761344 |