CHANGES IN THE SPLENIC EXTRACELLULAR MATRIX IN DOGS NATURALLY INFECTED WITH Leishmania (Leishmania) infantum chagasi
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Ciência animal brasileira (Online) |
Texto Completo: | https://revistas.ufg.br/vet/article/view/23500 |
Resumo: | The aim of this study was to study the changes in the splenic extracellular matrix of dogs naturally infected with Leishmania (Leishmania) infantum chagasi and its correlation to clinical, histopathological, and parasitological aspects. Eighteen dogs were used, separated into three groups: six non-infected animals (control group) and twelve infected animals. The dogs had undefined breed and age, from the township region of São Luís-MA. Paraffined slices of the spleen were stained with Hematoxilin and Eosin (H&E); Gomori’s ammoniacal Silver, to mark reticular fibers; and the Immunohistochemistry technique of streptavidin peroxidase to detect amastigote forms of Leishmania. The morphometrical analyses were done using the KS300 program and the images analysis system Kontron Elektronic/Carl Zeiss, Germany. The results showed that there is a significant increase in the deposition of collagen fibers in the spleen, compared to control animals, showing significant differences for symptomatic and asymptomatic animals. Positive correlations were found between the presence of the parasite in the tissue and collagen deposition. Symptomatic animals showed higher collagen deposition in the spleen, which can be associated to the high parasitism found in the tissue. The results showed that there is an intense fibrogenesis in the spleen in the canine visceral leishmaniasis, thus being associated to the parasitism of the tissue and the degenerative processes of the disease.Keywords: extracellular matrix; Leishmania; spleen. |
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CHANGES IN THE SPLENIC EXTRACELLULAR MATRIX IN DOGS NATURALLY INFECTED WITH Leishmania (Leishmania) infantum chagasiALTERAÇÕES DA MATRIZ EXTRACELULAR ESPLÊNICA EM CÃES NATURALMENTE INFECTADOS COM Leishmania (Leishmania) infantum chagasiAnimal Sanitybaçoleishmaniamatrix extracelularThe aim of this study was to study the changes in the splenic extracellular matrix of dogs naturally infected with Leishmania (Leishmania) infantum chagasi and its correlation to clinical, histopathological, and parasitological aspects. Eighteen dogs were used, separated into three groups: six non-infected animals (control group) and twelve infected animals. The dogs had undefined breed and age, from the township region of São Luís-MA. Paraffined slices of the spleen were stained with Hematoxilin and Eosin (H&E); Gomori’s ammoniacal Silver, to mark reticular fibers; and the Immunohistochemistry technique of streptavidin peroxidase to detect amastigote forms of Leishmania. The morphometrical analyses were done using the KS300 program and the images analysis system Kontron Elektronic/Carl Zeiss, Germany. The results showed that there is a significant increase in the deposition of collagen fibers in the spleen, compared to control animals, showing significant differences for symptomatic and asymptomatic animals. Positive correlations were found between the presence of the parasite in the tissue and collagen deposition. Symptomatic animals showed higher collagen deposition in the spleen, which can be associated to the high parasitism found in the tissue. The results showed that there is an intense fibrogenesis in the spleen in the canine visceral leishmaniasis, thus being associated to the parasitism of the tissue and the degenerative processes of the disease.Keywords: extracellular matrix; Leishmania; spleen. Objetivou-se estudar as alterações da matriz extracelular no baço de cães naturalmente infectados com Leishmania (Leishmania) infantum chagasi correlacionando-as com aspectos clínicos, histopatológicos e parasitológicos. Foram utilizados 18 cães, divididos em três grupos: seis animais não infectados (grupo controle) e doze animais infectados. Todos sem raça e idade definidas, provenientes da região do Município de São Luis-MA. Cortes parafinados do baço foram corados pela Hematoxilina e Eosina (H&E); Prata amoniacal de Gomori, para marcação das fibras reticulares e pela técnica Imuno-histoquímica da estreptoavidina-peroxidase para detecção de formas amastigotas de Leishmania. As análises morfométricas foram feitas utilizando-se o programa KS300 e o sistema de análise de imagens Kontron Elektronic/Carl Zeiss, Germany. Os resultados mostram que há um aumento significativo da deposição de fibras colágenas no baço quando comparadas aos animais controles, revelando diferenças significativas entre os animais sintomáticos e assintomáticos. Encontraram-se correlações positivas entre a presença do parasitismo tecidual e a deposição de colágeno. Os animais sintomáticos apresentaram uma maior deposição de colágeno no baço, que pode estar associada ao maior parasitismo tecidual encontrado. Os resultados demonstram que na leishmaniose visceral canina há uma fibrogênese intensa no baço, sendo esta associada ao parasitismo tecidual e a processos degenerativos decorrentes da doença.Palavras-chave: baço; Leishmania; matrix extracelular. Universidade Federal de Goiás2015-01-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionResearchAvaliado por paresPesquisa Científicaapplication/pdftext/htmlhttps://revistas.ufg.br/vet/article/view/2350010.1590/cab.v16i1.23500Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 16 No. 1 (2015); 103-115Ciência Animal Brasileira / Brazilian Animal Science; v. 16 n. 1 (2015); 103-1151809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/23500/17873https://revistas.ufg.br/vet/article/view/23500/17874Martins, Nathálya dos SantosCoelho, Glaucia Barbosados Santos, Larissa Sarmentode Oliveira, Rudson AlmeidaAbreu Silva, Ana LúciaMelo, Ferdinan Almeidainfo:eu-repo/semantics/openAccess2015-02-02T11:23:38Zoai:ojs.revistas.ufg.br:article/23500Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:55:58.503032Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true |
dc.title.none.fl_str_mv |
CHANGES IN THE SPLENIC EXTRACELLULAR MATRIX IN DOGS NATURALLY INFECTED WITH Leishmania (Leishmania) infantum chagasi ALTERAÇÕES DA MATRIZ EXTRACELULAR ESPLÊNICA EM CÃES NATURALMENTE INFECTADOS COM Leishmania (Leishmania) infantum chagasi |
title |
CHANGES IN THE SPLENIC EXTRACELLULAR MATRIX IN DOGS NATURALLY INFECTED WITH Leishmania (Leishmania) infantum chagasi |
spellingShingle |
CHANGES IN THE SPLENIC EXTRACELLULAR MATRIX IN DOGS NATURALLY INFECTED WITH Leishmania (Leishmania) infantum chagasi Martins, Nathálya dos Santos Animal Sanity baço leishmania matrix extracelular |
title_short |
CHANGES IN THE SPLENIC EXTRACELLULAR MATRIX IN DOGS NATURALLY INFECTED WITH Leishmania (Leishmania) infantum chagasi |
title_full |
CHANGES IN THE SPLENIC EXTRACELLULAR MATRIX IN DOGS NATURALLY INFECTED WITH Leishmania (Leishmania) infantum chagasi |
title_fullStr |
CHANGES IN THE SPLENIC EXTRACELLULAR MATRIX IN DOGS NATURALLY INFECTED WITH Leishmania (Leishmania) infantum chagasi |
title_full_unstemmed |
CHANGES IN THE SPLENIC EXTRACELLULAR MATRIX IN DOGS NATURALLY INFECTED WITH Leishmania (Leishmania) infantum chagasi |
title_sort |
CHANGES IN THE SPLENIC EXTRACELLULAR MATRIX IN DOGS NATURALLY INFECTED WITH Leishmania (Leishmania) infantum chagasi |
author |
Martins, Nathálya dos Santos |
author_facet |
Martins, Nathálya dos Santos Coelho, Glaucia Barbosa dos Santos, Larissa Sarmento de Oliveira, Rudson Almeida Abreu Silva, Ana Lúcia Melo, Ferdinan Almeida |
author_role |
author |
author2 |
Coelho, Glaucia Barbosa dos Santos, Larissa Sarmento de Oliveira, Rudson Almeida Abreu Silva, Ana Lúcia Melo, Ferdinan Almeida |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Martins, Nathálya dos Santos Coelho, Glaucia Barbosa dos Santos, Larissa Sarmento de Oliveira, Rudson Almeida Abreu Silva, Ana Lúcia Melo, Ferdinan Almeida |
dc.subject.por.fl_str_mv |
Animal Sanity baço leishmania matrix extracelular |
topic |
Animal Sanity baço leishmania matrix extracelular |
description |
The aim of this study was to study the changes in the splenic extracellular matrix of dogs naturally infected with Leishmania (Leishmania) infantum chagasi and its correlation to clinical, histopathological, and parasitological aspects. Eighteen dogs were used, separated into three groups: six non-infected animals (control group) and twelve infected animals. The dogs had undefined breed and age, from the township region of São Luís-MA. Paraffined slices of the spleen were stained with Hematoxilin and Eosin (H&E); Gomori’s ammoniacal Silver, to mark reticular fibers; and the Immunohistochemistry technique of streptavidin peroxidase to detect amastigote forms of Leishmania. The morphometrical analyses were done using the KS300 program and the images analysis system Kontron Elektronic/Carl Zeiss, Germany. The results showed that there is a significant increase in the deposition of collagen fibers in the spleen, compared to control animals, showing significant differences for symptomatic and asymptomatic animals. Positive correlations were found between the presence of the parasite in the tissue and collagen deposition. Symptomatic animals showed higher collagen deposition in the spleen, which can be associated to the high parasitism found in the tissue. The results showed that there is an intense fibrogenesis in the spleen in the canine visceral leishmaniasis, thus being associated to the parasitism of the tissue and the degenerative processes of the disease.Keywords: extracellular matrix; Leishmania; spleen. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-01-31 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Research Avaliado por pares Pesquisa Científica |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistas.ufg.br/vet/article/view/23500 10.1590/cab.v16i1.23500 |
url |
https://revistas.ufg.br/vet/article/view/23500 |
identifier_str_mv |
10.1590/cab.v16i1.23500 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://revistas.ufg.br/vet/article/view/23500/17873 https://revistas.ufg.br/vet/article/view/23500/17874 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 16 No. 1 (2015); 103-115 Ciência Animal Brasileira / Brazilian Animal Science; v. 16 n. 1 (2015); 103-115 1809-6891 1518-2797 reponame:Ciência animal brasileira (Online) instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Ciência animal brasileira (Online) |
collection |
Ciência animal brasileira (Online) |
repository.name.fl_str_mv |
Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
||revistacab@gmail.com |
_version_ |
1799874788121903104 |