IN SITU CULTURED PREANTRAL FOLLICLES IS A USEFUL MODEL TO EVALUATE THE EFFECT OF HOMEOPATHY MEDICINE ON EARLY FOLLICULOGENESIS

Detalhes bibliográficos
Autor(a) principal: da Cunha Nunes, Romão
Data de Publicação: 2018
Outros Autores: Ferreira de Lima, Laritza, Magalhães Pedrosa Rocha, Rebeca, Oliveira, Letícia, Cabral Campello, Claudio, Ricardo de Figueiredo, Jose
Tipo de documento: Artigo
Idioma: por
Título da fonte: Ciência animal brasileira (Online)
Texto Completo: https://revistas.ufg.br/vet/article/view/e-48658
Resumo: This study investigated the effect of homeopathic medicine (Pulsatilla nigricans and homeopathic follicle stimulating hormone - FSH) and the complex (Bos Stress Fertilis) on the initial folliculogenesis, using the in vitro culture of preantral follicles as in vitro model. For this, swine ovarian fragments were cultured for one or seven days in ?-MEM+ in the absence (cultured control) or presence of homeopathic FSH (6 cH), Pulsatilla (6 CH), Bos Stress Fertilis (6 CH), grain alcohol (50% v / v) or recombinant FSH (50 ng / ml) added daily. Uncultured ovarian fragments (fresh control) or cultured for one and seven days were processed for classical histology. Only the homeopathic complex Bos Stress Fertilis maintained the percentage of follicular survival after seven days of culture in relation to the uncultured control and ?-MEM+. Regarding follicular growth, only the addition of homeopathic FSH increased the follicular diameter when compared to the uncultured control and a-MEM+. Thus, it can be concluded that the addition of the homeopathic remedies Bos Stress fertilis and homeopathic FSH (6 CH) improved, respectively, survival and in vitro growth of swine preantral follicles included in ovarian tissue.Keywords: FSH; Homeopathy; Ovary; Preantral follicle; Swine
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spelling IN SITU CULTURED PREANTRAL FOLLICLES IS A USEFUL MODEL TO EVALUATE THE EFFECT OF HOMEOPATHY MEDICINE ON EARLY FOLLICULOGENESISUtilização do cultivo in vitro de folículos pré-antrais suínos inclusos em tecido ovariano (in situ) como modelo de avaliação da eficiência de medicamentos homeopáticos na foliculogênese inicialThis study investigated the effect of homeopathic medicine (Pulsatilla nigricans and homeopathic follicle stimulating hormone - FSH) and the complex (Bos Stress Fertilis) on the initial folliculogenesis, using the in vitro culture of preantral follicles as in vitro model. For this, swine ovarian fragments were cultured for one or seven days in ?-MEM+ in the absence (cultured control) or presence of homeopathic FSH (6 cH), Pulsatilla (6 CH), Bos Stress Fertilis (6 CH), grain alcohol (50% v / v) or recombinant FSH (50 ng / ml) added daily. Uncultured ovarian fragments (fresh control) or cultured for one and seven days were processed for classical histology. Only the homeopathic complex Bos Stress Fertilis maintained the percentage of follicular survival after seven days of culture in relation to the uncultured control and ?-MEM+. Regarding follicular growth, only the addition of homeopathic FSH increased the follicular diameter when compared to the uncultured control and a-MEM+. Thus, it can be concluded that the addition of the homeopathic remedies Bos Stress fertilis and homeopathic FSH (6 CH) improved, respectively, survival and in vitro growth of swine preantral follicles included in ovarian tissue.Keywords: FSH; Homeopathy; Ovary; Preantral follicle; SwineEste trabalho teve por objetivo avaliar o efeito dos medicamentos homeopáticos (Pulsatilla nigricans e hormônio folículo estimulante homeopático - FSH) e um complexo homeopático (Bos Stress Fertilis) na foliculogênese inicial, utilizando o cultivo in vitro de folículos pré-antrais suínos como modelo in vitro. Para tanto, fragmentos ovarianos foram cultivados por um ou sete dias em ?-MEM+ na ausência (controle cultivado) ou presença de FSH homeopático (6 cH), Pulsatilla (6 CH), Bos Stress Fertilis (6 CH), álcool cereal (50% - v/v) ou FSH recombinante (50 ng/ml) adicionados diariamente. Os fragmentos ovarianos não cultivados (controle fresco) ou cultivados por um e sete dias foram processados para histologia clássica. Somente o composto homeopático Bos Stress Fertilis foi eficiente em manter o percentual de sobrevivência folicular após sete dias de cultivo semelhante ao controle não cultivado e ?-MEM+. Em relação ao crescimento folicular, somente a adição de FSH homeopático aumentou o diâmetro folicular quando comparado ao controle não cultivado e ?-MEM+ após um dia de cultivo. Dessa forma, pode-se concluir que a adição dos medicamentos homeopáticos Bos Stress fertilis e FSH homeopático (6 CH) melhoraram, respectivamente, a sobrevivência e o crescimento in vitro de folículos pré-antrais suínos inclusos em fragmentos de tecido ovariano. Palavras-chave: Folículo pré-antral; FSH; Fomeopatia; Ovário; SuínoUniversidade Federal de Goiás2018-08-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/htmlhttps://revistas.ufg.br/vet/article/view/e-4865810.1590/cab19048658Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 19 (2018): Continuous publication; 1-8Ciência Animal Brasileira / Brazilian Animal Science; v. 19 (2018): Publicação contínua; 1-81809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/e-48658/25968https://revistas.ufg.br/vet/article/view/e-48658/25969Copyright (c) 2018 Ciência Animal Brasileirainfo:eu-repo/semantics/openAccessda Cunha Nunes, RomãoFerreira de Lima, LaritzaMagalhães Pedrosa Rocha, RebecaOliveira, LetíciaCabral Campello, ClaudioRicardo de Figueiredo, Jose2023-03-23T18:50:39Zoai:ojs.revistas.ufg.br:article/48658Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:56:19.607618Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true
dc.title.none.fl_str_mv IN SITU CULTURED PREANTRAL FOLLICLES IS A USEFUL MODEL TO EVALUATE THE EFFECT OF HOMEOPATHY MEDICINE ON EARLY FOLLICULOGENESIS
Utilização do cultivo in vitro de folículos pré-antrais suínos inclusos em tecido ovariano (in situ) como modelo de avaliação da eficiência de medicamentos homeopáticos na foliculogênese inicial
title IN SITU CULTURED PREANTRAL FOLLICLES IS A USEFUL MODEL TO EVALUATE THE EFFECT OF HOMEOPATHY MEDICINE ON EARLY FOLLICULOGENESIS
spellingShingle IN SITU CULTURED PREANTRAL FOLLICLES IS A USEFUL MODEL TO EVALUATE THE EFFECT OF HOMEOPATHY MEDICINE ON EARLY FOLLICULOGENESIS
da Cunha Nunes, Romão
title_short IN SITU CULTURED PREANTRAL FOLLICLES IS A USEFUL MODEL TO EVALUATE THE EFFECT OF HOMEOPATHY MEDICINE ON EARLY FOLLICULOGENESIS
title_full IN SITU CULTURED PREANTRAL FOLLICLES IS A USEFUL MODEL TO EVALUATE THE EFFECT OF HOMEOPATHY MEDICINE ON EARLY FOLLICULOGENESIS
title_fullStr IN SITU CULTURED PREANTRAL FOLLICLES IS A USEFUL MODEL TO EVALUATE THE EFFECT OF HOMEOPATHY MEDICINE ON EARLY FOLLICULOGENESIS
title_full_unstemmed IN SITU CULTURED PREANTRAL FOLLICLES IS A USEFUL MODEL TO EVALUATE THE EFFECT OF HOMEOPATHY MEDICINE ON EARLY FOLLICULOGENESIS
title_sort IN SITU CULTURED PREANTRAL FOLLICLES IS A USEFUL MODEL TO EVALUATE THE EFFECT OF HOMEOPATHY MEDICINE ON EARLY FOLLICULOGENESIS
author da Cunha Nunes, Romão
author_facet da Cunha Nunes, Romão
Ferreira de Lima, Laritza
Magalhães Pedrosa Rocha, Rebeca
Oliveira, Letícia
Cabral Campello, Claudio
Ricardo de Figueiredo, Jose
author_role author
author2 Ferreira de Lima, Laritza
Magalhães Pedrosa Rocha, Rebeca
Oliveira, Letícia
Cabral Campello, Claudio
Ricardo de Figueiredo, Jose
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv da Cunha Nunes, Romão
Ferreira de Lima, Laritza
Magalhães Pedrosa Rocha, Rebeca
Oliveira, Letícia
Cabral Campello, Claudio
Ricardo de Figueiredo, Jose
description This study investigated the effect of homeopathic medicine (Pulsatilla nigricans and homeopathic follicle stimulating hormone - FSH) and the complex (Bos Stress Fertilis) on the initial folliculogenesis, using the in vitro culture of preantral follicles as in vitro model. For this, swine ovarian fragments were cultured for one or seven days in ?-MEM+ in the absence (cultured control) or presence of homeopathic FSH (6 cH), Pulsatilla (6 CH), Bos Stress Fertilis (6 CH), grain alcohol (50% v / v) or recombinant FSH (50 ng / ml) added daily. Uncultured ovarian fragments (fresh control) or cultured for one and seven days were processed for classical histology. Only the homeopathic complex Bos Stress Fertilis maintained the percentage of follicular survival after seven days of culture in relation to the uncultured control and ?-MEM+. Regarding follicular growth, only the addition of homeopathic FSH increased the follicular diameter when compared to the uncultured control and a-MEM+. Thus, it can be concluded that the addition of the homeopathic remedies Bos Stress fertilis and homeopathic FSH (6 CH) improved, respectively, survival and in vitro growth of swine preantral follicles included in ovarian tissue.Keywords: FSH; Homeopathy; Ovary; Preantral follicle; Swine
publishDate 2018
dc.date.none.fl_str_mv 2018-08-02
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/vet/article/view/e-48658
10.1590/cab19048658
url https://revistas.ufg.br/vet/article/view/e-48658
identifier_str_mv 10.1590/cab19048658
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://revistas.ufg.br/vet/article/view/e-48658/25968
https://revistas.ufg.br/vet/article/view/e-48658/25969
dc.rights.driver.fl_str_mv Copyright (c) 2018 Ciência Animal Brasileira
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Ciência Animal Brasileira
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 19 (2018): Continuous publication; 1-8
Ciência Animal Brasileira / Brazilian Animal Science; v. 19 (2018): Publicação contínua; 1-8
1809-6891
1518-2797
reponame:Ciência animal brasileira (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Ciência animal brasileira (Online)
collection Ciência animal brasileira (Online)
repository.name.fl_str_mv Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv ||revistacab@gmail.com
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