ELECTROPHORETIC PROFILE OF SERUM AND PERITONEAL PROTEINS OF HORSES SUBMITTED TO EXPERIMENTAL OBSTRUCTION OF DUODENUM, ILEUM AND LARGE COLON

• Autor(a) principal: Di Filippo, Paula Alessandra
• Data de Publicação: 2010
• Outros Autores: Nogueira, Andressa Francisca da Silva, Anai, Letícia Abraao, Alves, Aracelle Elisane, Santana, Aureo Evangelista, Pereira, Gener Tadeu
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Ciência animal brasileira (Online)
• Texto Completo: https://revistas.ufg.br/vet/article/view/9699
• topic: Animal Sanity; cavalos; cólica; proteínas de fase aguda

Aiming at evaluating the electrophoresis profile of serum and peritoneal protein in horses submitted to an experimental model of intestinal obstruction, 24 animals were divided into four groups: instrumented control (GI), duodenum obstruction (GII), ileum obstruction (GIII) and large colon obstruction (GIV). Blood and peritoneal fluid samples were collected before the surgical procedures (T0), 3h after the obstruction (Ti) and 1, 3, 12, 24, 72, 120 and 168h after reperfusion (Tr). After sample centrifuging and fractioning, total protein were determined by biuret method, and the proteic fractions were separated by electrophoresis in agarose gel. Alterations were observed in serum and peritoneal concentrations of α, β and γ globulins on the studied animals. The changes occurred due to the intestinal inflammatory process caused by the obstruction model and the surgical trauma, associated to laparotomy. Animals submitted to duodenum and ileum obstruction showed higher serum and peritoneal concentrations of acute phase proteins. The electrophoretic separation of proteins included in the peritoneal liquid is more sensible when compared to the serum concentration to the diagnosis of abdominal inflammatory process.KEYWORDS: Acute phase protein, colic, horse.