Avaliação da atividade gastroprotetora do extrato etanólico das raízes da Me-mora nodosa (Silva Manso) Miers (Bignoniaceae) e de seu fitoconstituinte – Alantoína

Detalhes bibliográficos
Autor(a) principal: Silva, Dayane Moreira da
Data de Publicação: 2015
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFG
Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/9836
Resumo: Memora nodosa (Silva Manso) Miers (Bignoniaceae) is a Cerrado plant popularly known as caroba. In traditional medicine leaves and roots are used by the wound healing properties and the roots for abdominal pain. From ethanolic root extract of Memora nodosa (EERMN) was extracted a purinic derivative, allantoin, that is founded in products for topical use by the wound healing properties. Previous works revealed anti-inflammatory and antinociceptive properties with EERMN and allantoin. In this context, we seek evidence of whether, in addition to antiinflammatory action, there would be some gastric damaging effect. However, after the initial results obtained, this work sought to evaluate the antiulcerogenic effect of the extract and the allantoin and elicit the mechanisms involved in this activity. Albino Swiss mice, weighing 30-35g, maintained on standard conditions of temperature (23  1C) and illumination (12-h light/12-h dark cycle) were used. The gastroprotective activity was designed in different experimental gastric ulcers models induced by food restriction, indomethacin, stress, ethanol, ethanol acidity and acetic acid. Seven days after food restriction the animals treated with EERMN (300 mg/kg p.o.) showed reduction in the index of lesion from 15.01  2.92 to 5.20  1.44. In indomethacin induced gastric lesion, different EERMN doses (100, 300 and 1000 mg/kg p.o.) reduced the index of lesion from 12.70  1.70 (control) to 7.20  0.35; 6.37  0.41 and 5.71  0.47 respectively. Seven days after food restriction the animals treated with EERMN (300 mg/kg p. o.) showed reduction in the lesion index from 15.01  2.92 to 5.20  1.44. When the gastric ulcers were induced by ethanol 75%, the EERMN reduced the ulcerated area in 69%. The gastric acid secretion parameters (volume, pH and total acidity) did not alter by the treatment with the plant extract. However, the treatment with EERMN was capable to avoid the gastric mucus barrier depletion when compared to control lesion group, although it was not able to alter the glutathione levels. In the ethanol acidity model, the treatment with allantoin in doses of 30, 60 and 120 mg/kg reduced ulcerated area from 67.08  3.07% to 51.53  4.67; 42.32  4.93 and 43.18  4.90% respectively. When the lesions were induced by ethanol, allantoin (60 mg/kg p.o.) reduced ulcerated area in 80%. In models of acute lesions induced by stress and indomethacin, this same allantoin dose reduced the index of lesion from 11.90  0.93 to 6.62  0.49 and from 15.35  0.81 to 7.09  0.96 respectively. In the chronic model, allantoin demonstrated an antiulcerogenic effect by the reduction of lesion area from 74.41  4.57 to 24.10  0.89 mm2 in the acetic acid model. In the quantification of gastric mucus, similarly to EERMN, allantoin was able to prevent the mucus depletion (34.77  4.14) when compared with control lesion group (19.75  2.53). Moreover, allantoin was able to restore the activity of catalase enzyme from 403.7  15.1 (control lesion group) to 499.9  16.38 nmol/mg protein after gastric lesion induced by ethanol. These results demonstrate antiulcerogenic activity for EERMN and allantoin throughout different mechanisms.
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spelling Costa, Elson Alveshttp://lattes.cnpq.br/2607893423583912Costa, Elson AlvesPaula, José Realino deGhedini, Paulo Césarhttp://lattes.cnpq.br/1673571042121239Silva, Dayane Moreira da2019-07-16T12:39:25Z2015-07-10SILVA, Dayane Moreira da. Avaliação da atividade gastroprotetora do extrato etanólico das raízes da Me-mora nodosa (Silva Manso) Miers (Bignoniaceae) e de seu fitoconstituinte – Alantoína. 2015. 102 f. Dissertação (Mestrado em Ciências Farmacêuticas) - Universidade Federal de Goiás, Goiânia, 2015.http://repositorio.bc.ufg.br/tede/handle/tede/9836Memora nodosa (Silva Manso) Miers (Bignoniaceae) is a Cerrado plant popularly known as caroba. In traditional medicine leaves and roots are used by the wound healing properties and the roots for abdominal pain. From ethanolic root extract of Memora nodosa (EERMN) was extracted a purinic derivative, allantoin, that is founded in products for topical use by the wound healing properties. Previous works revealed anti-inflammatory and antinociceptive properties with EERMN and allantoin. In this context, we seek evidence of whether, in addition to antiinflammatory action, there would be some gastric damaging effect. However, after the initial results obtained, this work sought to evaluate the antiulcerogenic effect of the extract and the allantoin and elicit the mechanisms involved in this activity. Albino Swiss mice, weighing 30-35g, maintained on standard conditions of temperature (23  1C) and illumination (12-h light/12-h dark cycle) were used. The gastroprotective activity was designed in different experimental gastric ulcers models induced by food restriction, indomethacin, stress, ethanol, ethanol acidity and acetic acid. Seven days after food restriction the animals treated with EERMN (300 mg/kg p.o.) showed reduction in the index of lesion from 15.01  2.92 to 5.20  1.44. In indomethacin induced gastric lesion, different EERMN doses (100, 300 and 1000 mg/kg p.o.) reduced the index of lesion from 12.70  1.70 (control) to 7.20  0.35; 6.37  0.41 and 5.71  0.47 respectively. Seven days after food restriction the animals treated with EERMN (300 mg/kg p. o.) showed reduction in the lesion index from 15.01  2.92 to 5.20  1.44. When the gastric ulcers were induced by ethanol 75%, the EERMN reduced the ulcerated area in 69%. The gastric acid secretion parameters (volume, pH and total acidity) did not alter by the treatment with the plant extract. However, the treatment with EERMN was capable to avoid the gastric mucus barrier depletion when compared to control lesion group, although it was not able to alter the glutathione levels. In the ethanol acidity model, the treatment with allantoin in doses of 30, 60 and 120 mg/kg reduced ulcerated area from 67.08  3.07% to 51.53  4.67; 42.32  4.93 and 43.18  4.90% respectively. When the lesions were induced by ethanol, allantoin (60 mg/kg p.o.) reduced ulcerated area in 80%. In models of acute lesions induced by stress and indomethacin, this same allantoin dose reduced the index of lesion from 11.90  0.93 to 6.62  0.49 and from 15.35  0.81 to 7.09  0.96 respectively. In the chronic model, allantoin demonstrated an antiulcerogenic effect by the reduction of lesion area from 74.41  4.57 to 24.10  0.89 mm2 in the acetic acid model. In the quantification of gastric mucus, similarly to EERMN, allantoin was able to prevent the mucus depletion (34.77  4.14) when compared with control lesion group (19.75  2.53). Moreover, allantoin was able to restore the activity of catalase enzyme from 403.7  15.1 (control lesion group) to 499.9  16.38 nmol/mg protein after gastric lesion induced by ethanol. These results demonstrate antiulcerogenic activity for EERMN and allantoin throughout different mechanisms.Memora nodosa (Silva Manso) Miers (Bignoniaceae) é uma planta do Cerrado popularmente conhecida como caroba. Na medicina tradicional folhas e raízes da espécie são utilizadas pela propriedade cicatrizante de úlceras externas e as raízes para dores abdominais. Do extrato etanólico das raízes da M. nodosa (EERMN) foi extraído um derivado purínico, a alantoína, encontrada em produtos destinados ao uso tópico devido à sua propriedade cicatrizante. Além disto, dados da literatura demonstraram propriedades anti-inflamatória e antinociceptiva com o EERMN e com a alantoína. Desta maneira, avaliou-se o efeito promovido pelo EERMN e da alantoína sobre a mucosa gástrica de camundongos procurando evidenciar se, além da ação anti-inflamatória, haveria algum efeito lesivo sobre a mucosa gástrica. A partir dos resultados iniciais observados buscou-se avaliar o efeito antiulcerogênico do extrato etanólico e da alantoína buscando identificar os mecanismos de ação envolvidos nesta atividade. Foram utilizados camundongos albinos Swiss, pesando entre 30-35 g, mantidos em condições controladas de temperatura (23  1C) e luminosidade (ciclo claro-escuro de 12 horas). A atividade gastroprotetora foi avaliada em diferentes modelos experimentais de úlceras gástricas induzidas por restrição alimentar, indometacina, estresse, etanol, etanol acidificado e ácido acético. Após sete dias de restrição alimentar os animais tratados com EERMN (300 mg/kg v.o.) apresentaram redução no índice de lesão de 15.01  2.92 para 5.20  1.44. No modelo de lesão gástrica aguda induzida por indometacina, diferentes doses do EERMN (100, 300 e 1000 mg/kg v.o.) apresentaram redução no índice de lesão de 12.70  1.70 (controle) para 7.20  0.35; 6.37  0.41 e 5.71  0.47, respectivamente. Quando as lesões gástricas foram induzidas pelo estresse dos animais, o EERMN (300 mg/kg v.o.) promoveu a redução no índice de lesão de 9.10  1.05 para 5.20  0.24. Quando as úlceras gástricas foram induzidas por etanol a 75%, o EERMN foi capaz de reduzir a área ulcerada em 69%. Os parâmetros de secreção ácida gástrica (volume, acidez livre e total) não foram alterados pelo tratamento com o extrato. Entretanto, o tratamento com o EERMN foi capaz de evitar a depleção da barreira de muco gástrico em relação ao grupo controle lesado, embora não tenha sido capaz de alterar os níveis de glutationa. No modelo de indução por etanol acidificado, o tratamento com alantoína nas doses de 30, 60 e 120 mg/kg reduziu a área ulcerada de 67,08  3,07% para 51,53  4,67; 42,32  4,93 e 43,18  4,90%, respectivamente. Quando as lesões foram induzidas por etanol, alantoína (60 mg/kg v.o.) apresentou uma redução de 80% da área ulcerada. Nos modelos de lesões agudas induzidas por estresse e indometacina, esta mesma dose de alantoína foi capaz de reduzir o índice de lesão de 11,90  0,93 para 6,62  0,49 e de 15,35  0,81 para 7,09  0,96, respectivamente. Foi também demonstrado efeito antiulcerogênico visto pela redução da área de lesão de 74,41  4,57 para 24,10  0,89 mm2 no modelo de indução por ácido acético após sete dias de tratamento com alantoína em doses diárias de 60 mg/kg (v.o.). Na quantificação do muco gástrico, semelhantemente ao EERMN, a alantoína foi capaz de prevenir a depleção do muco fixado (34,77  4,14) em relação ao grupo controle lesado (19,75  2,53). Além disso, alantoína foi capaz de restaurar a atividade da enzima catalase de 403,7  15,13 (controle lesado) para 499,9  16,38 nmoles/mg de proteína após modelo de indução por etanol. Deste modo, o conjunto de resultados demonstra que a atividade gastroprotetora para o EERMN e alantoína pode envolver diferentes mecanismos.Submitted by Marlene Santos (marlene.bc.ufg@gmail.com) on 2019-07-15T20:39:34Z No. of bitstreams: 2 Dissertação - Dayane Moreira da Silva - 2015.pdf: 1693713 bytes, checksum: 5ab998f6981f95cb33755d513c05fea7 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2019-07-16T12:39:25Z (GMT) No. of bitstreams: 2 Dissertação - Dayane Moreira da Silva - 2015.pdf: 1693713 bytes, checksum: 5ab998f6981f95cb33755d513c05fea7 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2019-07-16T12:39:25Z (GMT). No. of bitstreams: 2 Dissertação - Dayane Moreira da Silva - 2015.pdf: 1693713 bytes, checksum: 5ab998f6981f95cb33755d513c05fea7 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2015-07-10Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Federal de GoiásPrograma de Pós-graduação em Ciências Farmacêuticas (FF)UFGBrasilFaculdade Farmácia - FF (RG)http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessMemora nodosaAlantoínaAntiulcerogênicaAllantoinAntiulcerogenicFARMACIA::ANALISE E CONTROLE E MEDICAMENTOSAvaliação da atividade gastroprotetora do extrato etanólico das raízes da Me-mora nodosa (Silva Manso) Miers (Bignoniaceae) e de seu fitoconstituinte – AlantoínaEvaluation of gastroprotective activity of ethanolic root extract of Memora nodosa (Silva Manso) Miers (Bignoniaceae) and of it phytoconstituent - Allantoininfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis824936988196152412600600600600601028116152420937562160250746569323362075167498588264571reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; 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dc.title.eng.fl_str_mv Avaliação da atividade gastroprotetora do extrato etanólico das raízes da Me-mora nodosa (Silva Manso) Miers (Bignoniaceae) e de seu fitoconstituinte – Alantoína
dc.title.alternative.eng.fl_str_mv Evaluation of gastroprotective activity of ethanolic root extract of Memora nodosa (Silva Manso) Miers (Bignoniaceae) and of it phytoconstituent - Allantoin
title Avaliação da atividade gastroprotetora do extrato etanólico das raízes da Me-mora nodosa (Silva Manso) Miers (Bignoniaceae) e de seu fitoconstituinte – Alantoína
spellingShingle Avaliação da atividade gastroprotetora do extrato etanólico das raízes da Me-mora nodosa (Silva Manso) Miers (Bignoniaceae) e de seu fitoconstituinte – Alantoína
Silva, Dayane Moreira da
Memora nodosa
Alantoína
Antiulcerogênica
Allantoin
Antiulcerogenic
FARMACIA::ANALISE E CONTROLE E MEDICAMENTOS
title_short Avaliação da atividade gastroprotetora do extrato etanólico das raízes da Me-mora nodosa (Silva Manso) Miers (Bignoniaceae) e de seu fitoconstituinte – Alantoína
title_full Avaliação da atividade gastroprotetora do extrato etanólico das raízes da Me-mora nodosa (Silva Manso) Miers (Bignoniaceae) e de seu fitoconstituinte – Alantoína
title_fullStr Avaliação da atividade gastroprotetora do extrato etanólico das raízes da Me-mora nodosa (Silva Manso) Miers (Bignoniaceae) e de seu fitoconstituinte – Alantoína
title_full_unstemmed Avaliação da atividade gastroprotetora do extrato etanólico das raízes da Me-mora nodosa (Silva Manso) Miers (Bignoniaceae) e de seu fitoconstituinte – Alantoína
title_sort Avaliação da atividade gastroprotetora do extrato etanólico das raízes da Me-mora nodosa (Silva Manso) Miers (Bignoniaceae) e de seu fitoconstituinte – Alantoína
author Silva, Dayane Moreira da
author_facet Silva, Dayane Moreira da
author_role author
dc.contributor.advisor1.fl_str_mv Costa, Elson Alves
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/2607893423583912
dc.contributor.referee1.fl_str_mv Costa, Elson Alves
dc.contributor.referee2.fl_str_mv Paula, José Realino de
dc.contributor.referee3.fl_str_mv Ghedini, Paulo César
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/1673571042121239
dc.contributor.author.fl_str_mv Silva, Dayane Moreira da
contributor_str_mv Costa, Elson Alves
Costa, Elson Alves
Paula, José Realino de
Ghedini, Paulo César
dc.subject.por.fl_str_mv Memora nodosa
Alantoína
Antiulcerogênica
Allantoin
Antiulcerogenic
topic Memora nodosa
Alantoína
Antiulcerogênica
Allantoin
Antiulcerogenic
FARMACIA::ANALISE E CONTROLE E MEDICAMENTOS
dc.subject.cnpq.fl_str_mv FARMACIA::ANALISE E CONTROLE E MEDICAMENTOS
description Memora nodosa (Silva Manso) Miers (Bignoniaceae) is a Cerrado plant popularly known as caroba. In traditional medicine leaves and roots are used by the wound healing properties and the roots for abdominal pain. From ethanolic root extract of Memora nodosa (EERMN) was extracted a purinic derivative, allantoin, that is founded in products for topical use by the wound healing properties. Previous works revealed anti-inflammatory and antinociceptive properties with EERMN and allantoin. In this context, we seek evidence of whether, in addition to antiinflammatory action, there would be some gastric damaging effect. However, after the initial results obtained, this work sought to evaluate the antiulcerogenic effect of the extract and the allantoin and elicit the mechanisms involved in this activity. Albino Swiss mice, weighing 30-35g, maintained on standard conditions of temperature (23  1C) and illumination (12-h light/12-h dark cycle) were used. The gastroprotective activity was designed in different experimental gastric ulcers models induced by food restriction, indomethacin, stress, ethanol, ethanol acidity and acetic acid. Seven days after food restriction the animals treated with EERMN (300 mg/kg p.o.) showed reduction in the index of lesion from 15.01  2.92 to 5.20  1.44. In indomethacin induced gastric lesion, different EERMN doses (100, 300 and 1000 mg/kg p.o.) reduced the index of lesion from 12.70  1.70 (control) to 7.20  0.35; 6.37  0.41 and 5.71  0.47 respectively. Seven days after food restriction the animals treated with EERMN (300 mg/kg p. o.) showed reduction in the lesion index from 15.01  2.92 to 5.20  1.44. When the gastric ulcers were induced by ethanol 75%, the EERMN reduced the ulcerated area in 69%. The gastric acid secretion parameters (volume, pH and total acidity) did not alter by the treatment with the plant extract. However, the treatment with EERMN was capable to avoid the gastric mucus barrier depletion when compared to control lesion group, although it was not able to alter the glutathione levels. In the ethanol acidity model, the treatment with allantoin in doses of 30, 60 and 120 mg/kg reduced ulcerated area from 67.08  3.07% to 51.53  4.67; 42.32  4.93 and 43.18  4.90% respectively. When the lesions were induced by ethanol, allantoin (60 mg/kg p.o.) reduced ulcerated area in 80%. In models of acute lesions induced by stress and indomethacin, this same allantoin dose reduced the index of lesion from 11.90  0.93 to 6.62  0.49 and from 15.35  0.81 to 7.09  0.96 respectively. In the chronic model, allantoin demonstrated an antiulcerogenic effect by the reduction of lesion area from 74.41  4.57 to 24.10  0.89 mm2 in the acetic acid model. In the quantification of gastric mucus, similarly to EERMN, allantoin was able to prevent the mucus depletion (34.77  4.14) when compared with control lesion group (19.75  2.53). Moreover, allantoin was able to restore the activity of catalase enzyme from 403.7  15.1 (control lesion group) to 499.9  16.38 nmol/mg protein after gastric lesion induced by ethanol. These results demonstrate antiulcerogenic activity for EERMN and allantoin throughout different mechanisms.
publishDate 2015
dc.date.issued.fl_str_mv 2015-07-10
dc.date.accessioned.fl_str_mv 2019-07-16T12:39:25Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
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dc.identifier.citation.fl_str_mv SILVA, Dayane Moreira da. Avaliação da atividade gastroprotetora do extrato etanólico das raízes da Me-mora nodosa (Silva Manso) Miers (Bignoniaceae) e de seu fitoconstituinte – Alantoína. 2015. 102 f. Dissertação (Mestrado em Ciências Farmacêuticas) - Universidade Federal de Goiás, Goiânia, 2015.
dc.identifier.uri.fl_str_mv http://repositorio.bc.ufg.br/tede/handle/tede/9836
identifier_str_mv SILVA, Dayane Moreira da. Avaliação da atividade gastroprotetora do extrato etanólico das raízes da Me-mora nodosa (Silva Manso) Miers (Bignoniaceae) e de seu fitoconstituinte – Alantoína. 2015. 102 f. Dissertação (Mestrado em Ciências Farmacêuticas) - Universidade Federal de Goiás, Goiânia, 2015.
url http://repositorio.bc.ufg.br/tede/handle/tede/9836
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language por
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dc.relation.sponsorship.fl_str_mv 2075167498588264571
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
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dc.publisher.none.fl_str_mv Universidade Federal de Goiás
dc.publisher.program.fl_str_mv Programa de Pós-graduação em Ciências Farmacêuticas (FF)
dc.publisher.initials.fl_str_mv UFG
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Faculdade Farmácia - FF (RG)
publisher.none.fl_str_mv Universidade Federal de Goiás
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