SNPs e microssatélites na caracterização racial e de resistência a enfermidades em bovinos da raça Curraleiro Pé-Duro

• Autor(a) principal: Freitas, Thais Miranda Silva
• Data de Publicação: 2018
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Repositório Institucional da UFG
• Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/9838
• topic: Gene BoLA; Marcadores moleculares; Raça local brasileira; BoLA gene; Brazilian cattle breed; Molecular markers; CIENCIA E TECNOLOGIA DE ALIMENTOS::TECNOLOGIA DE ALIMENTOS

The Brazilian cattle breed Curraleiro Pé-Duro is an important genetic resource that has as characteristics of rusticity, adaptability to environmental conditions, and resistance to diseases and intoxications. This study aims to analyze the genetic diversity of Curraleiro Pé-Duro populations and verify the presence of SNPs and microsatellite polymorphisms for breed characterization and disease resistanceto Brucella abortus, Leptospira spp, Neospora caninum, leukosis virus, Infectious Bovine Rhinotracheitis, and Bovine Viral Diarrhea. A total of 1.182 serum and blood samples of Curraleiro Pé-Duro breed belonging to the Pro-Centro-Oeste Network database were used. Amplification and genotyping of 28 microsatellite markers of breed characterization and 13 markers related to the bovine leukocyte antigen (BoLA) and SLC11A genes were used. Twenty-four single-base polymorphisms (SNPs), detected by Kompetitive Allele-Specific PCR, were investigated in genes related to cytokines expression. Univariate analysis was performed for the association of genotypes with phenotypes, followed by logistic regression considering a significance level of 5%. Bayesian analysis grouped the animals into three breed clusters, indicating the presence of subpopulation, with similar prevalence for infections. Heterozygosity and Fst values indicated little differentiation between clusters and increased homozygosity. On BoLA gene, 42 allels associated to infections were identified. SNP haplotypes in BTA6 and BTA15 were significant in seronegative animals while BTA17 haplotype 2 was presented in 70% of Neospora caninum positive animals. The results showed that Curraleiro Pé-Duro cattle breed has alleles of resistance to infections that can be lost due to loss of genetic diversity.