IN VITRO OF Melanoxylon brauna SCHOTT. MORPHOGENESIS: RESPONSIVENESS OF EXPLANTS TO PERMANENT AND TEMPORARY IMMERSION GROWTH REGULATORS
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Cerne (Online) |
Texto Completo: | https://cerne.ufla.br/site/index.php/CERNE/article/view/2372 |
Resumo: | Melanoxylon brauna is propagated by seeds, however, low yield, pest attack and short viability are problems in seedling production. Plant tissue culture is an alternative for large-scale seedling production, but studies with this species are scarce. The objective of this study was to study in vitro morphogenesis of M. brauna after induction of growth regulators. Three experiments were performed: I. In vitro bud proliferation of juvenile M. brauna explants at different BAP concentrations. The number and length of shoots (mm) was analyzed; bud explants-1 (%); calogenesis (%) and direct and indirect organogenesis budding-1 (%). II. In vitro rhizogenesis of M. brauna shoots with different types and auxin concentrations; III. In vitro rhizogenesis of M. brauna under temporary immersion of shoots at different IBA concentrations. Calogenesis (%) was analyzed; rhizogenesis (%); number, length (mm) and root dry mass (mg). The nodal segment with two axillary buds was the most responsive explant in the formation of shoots with BAP (1 mg L-1). The auxin concentrations of IBA, ANA and 2,4-D were not favorable to rhizogenesis in MS culture medium. However, IBA concentrations of 2544.81 and 2607.52 mg L-1 promoted superior results in rhizogenesis and shoot emission, respectively, when submitted to explant bases in temporary immersion in this auxin and planted in vermiculite substrate. |
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IN VITRO OF Melanoxylon brauna SCHOTT. MORPHOGENESIS: RESPONSIVENESS OF EXPLANTS TO PERMANENT AND TEMPORARY IMMERSION GROWTH REGULATORSMORFOGÊNESE IN VITRO DE BRAÚNA: RESPONSIVIDADE DE EXPLANTES À REGULADORES DE CRESCIMENTO POR IMERSÃO PERMANENTE E TEMPORÁRIAbrauna, tissue culture, polarity, cytokine, auxinsMelanoxylon brauna is propagated by seeds, however, low yield, pest attack and short viability are problems in seedling production. Plant tissue culture is an alternative for large-scale seedling production, but studies with this species are scarce. The objective of this study was to study in vitro morphogenesis of M. brauna after induction of growth regulators. Three experiments were performed: I. In vitro bud proliferation of juvenile M. brauna explants at different BAP concentrations. The number and length of shoots (mm) was analyzed; bud explants-1 (%); calogenesis (%) and direct and indirect organogenesis budding-1 (%). II. In vitro rhizogenesis of M. brauna shoots with different types and auxin concentrations; III. In vitro rhizogenesis of M. brauna under temporary immersion of shoots at different IBA concentrations. Calogenesis (%) was analyzed; rhizogenesis (%); number, length (mm) and root dry mass (mg). The nodal segment with two axillary buds was the most responsive explant in the formation of shoots with BAP (1 mg L-1). The auxin concentrations of IBA, ANA and 2,4-D were not favorable to rhizogenesis in MS culture medium. However, IBA concentrations of 2544.81 and 2607.52 mg L-1 promoted superior results in rhizogenesis and shoot emission, respectively, when submitted to explant bases in temporary immersion in this auxin and planted in vermiculite substrate.CERNECERNE2020-05-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://cerne.ufla.br/site/index.php/CERNE/article/view/2372CERNE; Vol. 26 No. 1 (2020); 26-36CERNE; v. 26 n. 1 (2020); 26-362317-63420104-7760reponame:Cerne (Online)instname:Universidade Federal de Lavras (UFLA)instacron:UFLAenghttps://cerne.ufla.br/site/index.php/CERNE/article/view/2372/1170Copyright (c) 2020 CERNEinfo:eu-repo/semantics/openAccessSilva, Elisa Regina daSimões, Ingridh MedeirosBaptista, Julcinara OliveiraSchmildt, Edilson RomaisLopes, José CarlosGonçalves, Elzimar de OliveiraCaldeira, Marcos Vinícius WincklerAlexandre, Rodrigo Sobreira2020-05-15T19:57:22Zoai:cerne.ufla.br:article/2372Revistahttps://cerne.ufla.br/site/index.php/CERNEPUBhttps://cerne.ufla.br/site/index.php/CERNE/oaicerne@dcf.ufla.br||cerne@dcf.ufla.br2317-63420104-7760opendoar:2024-05-21T19:54:44.331527Cerne (Online) - Universidade Federal de Lavras (UFLA)true |
dc.title.none.fl_str_mv |
IN VITRO OF Melanoxylon brauna SCHOTT. MORPHOGENESIS: RESPONSIVENESS OF EXPLANTS TO PERMANENT AND TEMPORARY IMMERSION GROWTH REGULATORS MORFOGÊNESE IN VITRO DE BRAÚNA: RESPONSIVIDADE DE EXPLANTES À REGULADORES DE CRESCIMENTO POR IMERSÃO PERMANENTE E TEMPORÁRIA |
title |
IN VITRO OF Melanoxylon brauna SCHOTT. MORPHOGENESIS: RESPONSIVENESS OF EXPLANTS TO PERMANENT AND TEMPORARY IMMERSION GROWTH REGULATORS |
spellingShingle |
IN VITRO OF Melanoxylon brauna SCHOTT. MORPHOGENESIS: RESPONSIVENESS OF EXPLANTS TO PERMANENT AND TEMPORARY IMMERSION GROWTH REGULATORS Silva, Elisa Regina da brauna, tissue culture, polarity, cytokine, auxins |
title_short |
IN VITRO OF Melanoxylon brauna SCHOTT. MORPHOGENESIS: RESPONSIVENESS OF EXPLANTS TO PERMANENT AND TEMPORARY IMMERSION GROWTH REGULATORS |
title_full |
IN VITRO OF Melanoxylon brauna SCHOTT. MORPHOGENESIS: RESPONSIVENESS OF EXPLANTS TO PERMANENT AND TEMPORARY IMMERSION GROWTH REGULATORS |
title_fullStr |
IN VITRO OF Melanoxylon brauna SCHOTT. MORPHOGENESIS: RESPONSIVENESS OF EXPLANTS TO PERMANENT AND TEMPORARY IMMERSION GROWTH REGULATORS |
title_full_unstemmed |
IN VITRO OF Melanoxylon brauna SCHOTT. MORPHOGENESIS: RESPONSIVENESS OF EXPLANTS TO PERMANENT AND TEMPORARY IMMERSION GROWTH REGULATORS |
title_sort |
IN VITRO OF Melanoxylon brauna SCHOTT. MORPHOGENESIS: RESPONSIVENESS OF EXPLANTS TO PERMANENT AND TEMPORARY IMMERSION GROWTH REGULATORS |
author |
Silva, Elisa Regina da |
author_facet |
Silva, Elisa Regina da Simões, Ingridh Medeiros Baptista, Julcinara Oliveira Schmildt, Edilson Romais Lopes, José Carlos Gonçalves, Elzimar de Oliveira Caldeira, Marcos Vinícius Winckler Alexandre, Rodrigo Sobreira |
author_role |
author |
author2 |
Simões, Ingridh Medeiros Baptista, Julcinara Oliveira Schmildt, Edilson Romais Lopes, José Carlos Gonçalves, Elzimar de Oliveira Caldeira, Marcos Vinícius Winckler Alexandre, Rodrigo Sobreira |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Silva, Elisa Regina da Simões, Ingridh Medeiros Baptista, Julcinara Oliveira Schmildt, Edilson Romais Lopes, José Carlos Gonçalves, Elzimar de Oliveira Caldeira, Marcos Vinícius Winckler Alexandre, Rodrigo Sobreira |
dc.subject.por.fl_str_mv |
brauna, tissue culture, polarity, cytokine, auxins |
topic |
brauna, tissue culture, polarity, cytokine, auxins |
description |
Melanoxylon brauna is propagated by seeds, however, low yield, pest attack and short viability are problems in seedling production. Plant tissue culture is an alternative for large-scale seedling production, but studies with this species are scarce. The objective of this study was to study in vitro morphogenesis of M. brauna after induction of growth regulators. Three experiments were performed: I. In vitro bud proliferation of juvenile M. brauna explants at different BAP concentrations. The number and length of shoots (mm) was analyzed; bud explants-1 (%); calogenesis (%) and direct and indirect organogenesis budding-1 (%). II. In vitro rhizogenesis of M. brauna shoots with different types and auxin concentrations; III. In vitro rhizogenesis of M. brauna under temporary immersion of shoots at different IBA concentrations. Calogenesis (%) was analyzed; rhizogenesis (%); number, length (mm) and root dry mass (mg). The nodal segment with two axillary buds was the most responsive explant in the formation of shoots with BAP (1 mg L-1). The auxin concentrations of IBA, ANA and 2,4-D were not favorable to rhizogenesis in MS culture medium. However, IBA concentrations of 2544.81 and 2607.52 mg L-1 promoted superior results in rhizogenesis and shoot emission, respectively, when submitted to explant bases in temporary immersion in this auxin and planted in vermiculite substrate. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-05-12 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://cerne.ufla.br/site/index.php/CERNE/article/view/2372 |
url |
https://cerne.ufla.br/site/index.php/CERNE/article/view/2372 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://cerne.ufla.br/site/index.php/CERNE/article/view/2372/1170 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2020 CERNE info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2020 CERNE |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
CERNE CERNE |
publisher.none.fl_str_mv |
CERNE CERNE |
dc.source.none.fl_str_mv |
CERNE; Vol. 26 No. 1 (2020); 26-36 CERNE; v. 26 n. 1 (2020); 26-36 2317-6342 0104-7760 reponame:Cerne (Online) instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Cerne (Online) |
collection |
Cerne (Online) |
repository.name.fl_str_mv |
Cerne (Online) - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
cerne@dcf.ufla.br||cerne@dcf.ufla.br |
_version_ |
1799874944146866176 |