Seed treatment with prodigiosin controls damping-off of cucumber caused by Pythium ultimum
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/49008 |
Resumo: | Ethanol extract of cell mass of Serratia marcescens strain N4-5, when applied as a treatment to cucumber seed, has been shown to provide control of the oomycete soil-borne plant pathogen Pythium ultimum equivalent to that provided by a seed-treatment chemical pesticide in some soils. Two dominant compounds in this extract, prodigiosin and the serratamolide serrawetin W1, were identified based on mass and collision induced dissociation mass fragmentation spectra. An additional four compounds with M+H+ masses (487, 541, 543, and 571) consistent with serratamolides reported in the literature were also detected. Several other compounds with M+H+ masses of 488, 536, 684, 834, 906, and 908 m/z were detected in this ethanol extract inconsistently over multiple liquid chromatography coupled with tandem mass spectrometry (LC/MS–MS) runs. A purified preparation of prodigiosin provided control of damping-off of cucumber caused by P. ultimum when applied as a seed treatment while ethanol extract of cell mass of strain Tn246, a transposon-mutant-derivative of strain N4-5, did not. Strain Tn246 contained a mini-Tn5 Km insertion in a prodigiosin biosynthetic gene and was deficient in production of prodigiosin. All other compounds detected in N4-5 extract were detected in the Tn246 extract. This is the first report demonstrating that prodigiosin can control a plant disease. Other compounds in ethanol extract of strain N4-5 may contribute to disease control. |
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Seed treatment with prodigiosin controls damping-off of cucumber caused by Pythium ultimumNatural productProdigiosinPythium ultimumSerratamolidesSerratia marcescensSerrawettinEthanol extract of cell mass of Serratia marcescens strain N4-5, when applied as a treatment to cucumber seed, has been shown to provide control of the oomycete soil-borne plant pathogen Pythium ultimum equivalent to that provided by a seed-treatment chemical pesticide in some soils. Two dominant compounds in this extract, prodigiosin and the serratamolide serrawetin W1, were identified based on mass and collision induced dissociation mass fragmentation spectra. An additional four compounds with M+H+ masses (487, 541, 543, and 571) consistent with serratamolides reported in the literature were also detected. Several other compounds with M+H+ masses of 488, 536, 684, 834, 906, and 908 m/z were detected in this ethanol extract inconsistently over multiple liquid chromatography coupled with tandem mass spectrometry (LC/MS–MS) runs. A purified preparation of prodigiosin provided control of damping-off of cucumber caused by P. ultimum when applied as a seed treatment while ethanol extract of cell mass of strain Tn246, a transposon-mutant-derivative of strain N4-5, did not. Strain Tn246 contained a mini-Tn5 Km insertion in a prodigiosin biosynthetic gene and was deficient in production of prodigiosin. All other compounds detected in N4-5 extract were detected in the Tn246 extract. This is the first report demonstrating that prodigiosin can control a plant disease. Other compounds in ethanol extract of strain N4-5 may contribute to disease control.Springer2022-01-25T00:57:49Z2022-01-25T00:57:49Z2021-01-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfROBERTS, D. P. et al. Seed treatment with prodigiosin controls damping-off of cucumber caused by Pythium ultimum. AMB Express, [S.l.], v. 11, n. 10, p. 1-9, Jan. 2021. DOI: 10.1186/s13568-020-01169-2.http://repositorio.ufla.br/jspui/handle/1/49008AMB Expressreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessRoberts, Daniel P.Selmer, KaitlynLupitskyy, RobertRice, ClifordBuyer, Jefrey S.Maul, Jude E.Lakshman, Dilip K.Souza, Jorge deeng2023-05-26T19:51:58Zoai:localhost:1/49008Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-05-26T19:51:58Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Seed treatment with prodigiosin controls damping-off of cucumber caused by Pythium ultimum |
title |
Seed treatment with prodigiosin controls damping-off of cucumber caused by Pythium ultimum |
spellingShingle |
Seed treatment with prodigiosin controls damping-off of cucumber caused by Pythium ultimum Roberts, Daniel P. Natural product Prodigiosin Pythium ultimum Serratamolides Serratia marcescens Serrawettin |
title_short |
Seed treatment with prodigiosin controls damping-off of cucumber caused by Pythium ultimum |
title_full |
Seed treatment with prodigiosin controls damping-off of cucumber caused by Pythium ultimum |
title_fullStr |
Seed treatment with prodigiosin controls damping-off of cucumber caused by Pythium ultimum |
title_full_unstemmed |
Seed treatment with prodigiosin controls damping-off of cucumber caused by Pythium ultimum |
title_sort |
Seed treatment with prodigiosin controls damping-off of cucumber caused by Pythium ultimum |
author |
Roberts, Daniel P. |
author_facet |
Roberts, Daniel P. Selmer, Kaitlyn Lupitskyy, Robert Rice, Cliford Buyer, Jefrey S. Maul, Jude E. Lakshman, Dilip K. Souza, Jorge de |
author_role |
author |
author2 |
Selmer, Kaitlyn Lupitskyy, Robert Rice, Cliford Buyer, Jefrey S. Maul, Jude E. Lakshman, Dilip K. Souza, Jorge de |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Roberts, Daniel P. Selmer, Kaitlyn Lupitskyy, Robert Rice, Cliford Buyer, Jefrey S. Maul, Jude E. Lakshman, Dilip K. Souza, Jorge de |
dc.subject.por.fl_str_mv |
Natural product Prodigiosin Pythium ultimum Serratamolides Serratia marcescens Serrawettin |
topic |
Natural product Prodigiosin Pythium ultimum Serratamolides Serratia marcescens Serrawettin |
description |
Ethanol extract of cell mass of Serratia marcescens strain N4-5, when applied as a treatment to cucumber seed, has been shown to provide control of the oomycete soil-borne plant pathogen Pythium ultimum equivalent to that provided by a seed-treatment chemical pesticide in some soils. Two dominant compounds in this extract, prodigiosin and the serratamolide serrawetin W1, were identified based on mass and collision induced dissociation mass fragmentation spectra. An additional four compounds with M+H+ masses (487, 541, 543, and 571) consistent with serratamolides reported in the literature were also detected. Several other compounds with M+H+ masses of 488, 536, 684, 834, 906, and 908 m/z were detected in this ethanol extract inconsistently over multiple liquid chromatography coupled with tandem mass spectrometry (LC/MS–MS) runs. A purified preparation of prodigiosin provided control of damping-off of cucumber caused by P. ultimum when applied as a seed treatment while ethanol extract of cell mass of strain Tn246, a transposon-mutant-derivative of strain N4-5, did not. Strain Tn246 contained a mini-Tn5 Km insertion in a prodigiosin biosynthetic gene and was deficient in production of prodigiosin. All other compounds detected in N4-5 extract were detected in the Tn246 extract. This is the first report demonstrating that prodigiosin can control a plant disease. Other compounds in ethanol extract of strain N4-5 may contribute to disease control. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-06 2022-01-25T00:57:49Z 2022-01-25T00:57:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
ROBERTS, D. P. et al. Seed treatment with prodigiosin controls damping-off of cucumber caused by Pythium ultimum. AMB Express, [S.l.], v. 11, n. 10, p. 1-9, Jan. 2021. DOI: 10.1186/s13568-020-01169-2. http://repositorio.ufla.br/jspui/handle/1/49008 |
identifier_str_mv |
ROBERTS, D. P. et al. Seed treatment with prodigiosin controls damping-off of cucumber caused by Pythium ultimum. AMB Express, [S.l.], v. 11, n. 10, p. 1-9, Jan. 2021. DOI: 10.1186/s13568-020-01169-2. |
url |
http://repositorio.ufla.br/jspui/handle/1/49008 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
AMB Express reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
_version_ |
1807835226475331584 |