Effects of the ration of linoleic acid to linolenic acid dietary to tilapia females (Oreochromis niloticus)
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Tipo de documento: | Tese |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/29061 |
Resumo: | Polyunsaturated fatty acids (PUFAs) and their long chain derivatives (LCPUFA) are involved in various functions in the body. However, an adequate balance between dietary n-3 and n-6 fatty acids (FA) from plant sources, may prevent possible competition in their biosynthetic routes. The objective of this work, was to evaluate the effect of different dietary linoleic acid/ linolenic acid (LA/ LNA) ratio for Nile tilapia (Oreochromis niloticus) breeding females. An experiment was performed using 24 males and 72 females with mean initial weight of 131.3 g and 124.4 g, respectively. Six females and two males were distributed in twelve tanks of 250L each. The design was completely randomized, with four treatments (20.1, 4.5, 3.9 and 0.7 LA / LNA) and three replications. Observations were made for the occurrence of spawning every day. At the end of the test, blood and tissue samples were collected. Fish fed the 3.9 and 0.7 LA/LNA diets had higher number of eggs produced (p<0.05). Hatch rates and larval survival were not affected by the treatments (p>0.05). Specific growth rate was highest in fish fed the 0.7 LA/LNA ratio and hepatosomatic index was highest in fish fed the 4.5 LA/LNA ratio (p<0.05). In general, the FA profile of gonads reflected the composition of the diet. The arachidonic acid content was higher in 20.1 ratio and the eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids concentration were higher in 0.7 ratio (p<0,05). Diets did not influence the triglycerides content in plasma (p>0.05). However, higher levels of cholesterol were observed in fish fed 4.5 and 3.9 LA/ LNA ratio (p<0.05). The highest dietary LA levels increased the gene expression of cyp17, while the dietary 3.9 LA/ LNA ratio promoted highest expression of cyp19a1a (p<0.05). In conclusion, a 3.9 LA/ LNA ratio should be used for Nile tilapia female. |
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Effects of the ration of linoleic acid to linolenic acid dietary to tilapia females (Oreochromis niloticus)Efeitos da relação ácido linoleico/ ácido linolênico dietéticos para fêmeas de tilápia do Nilo (Oreochromisniloticus)Ácidos graxos poli-insaturadosTilápia - NutriçãoLipídiosFatty acidsTilapia - NutritionLipidsZootecniaPolyunsaturated fatty acids (PUFAs) and their long chain derivatives (LCPUFA) are involved in various functions in the body. However, an adequate balance between dietary n-3 and n-6 fatty acids (FA) from plant sources, may prevent possible competition in their biosynthetic routes. The objective of this work, was to evaluate the effect of different dietary linoleic acid/ linolenic acid (LA/ LNA) ratio for Nile tilapia (Oreochromis niloticus) breeding females. An experiment was performed using 24 males and 72 females with mean initial weight of 131.3 g and 124.4 g, respectively. Six females and two males were distributed in twelve tanks of 250L each. The design was completely randomized, with four treatments (20.1, 4.5, 3.9 and 0.7 LA / LNA) and three replications. Observations were made for the occurrence of spawning every day. At the end of the test, blood and tissue samples were collected. Fish fed the 3.9 and 0.7 LA/LNA diets had higher number of eggs produced (p<0.05). Hatch rates and larval survival were not affected by the treatments (p>0.05). Specific growth rate was highest in fish fed the 0.7 LA/LNA ratio and hepatosomatic index was highest in fish fed the 4.5 LA/LNA ratio (p<0.05). In general, the FA profile of gonads reflected the composition of the diet. The arachidonic acid content was higher in 20.1 ratio and the eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids concentration were higher in 0.7 ratio (p<0,05). Diets did not influence the triglycerides content in plasma (p>0.05). However, higher levels of cholesterol were observed in fish fed 4.5 and 3.9 LA/ LNA ratio (p<0.05). The highest dietary LA levels increased the gene expression of cyp17, while the dietary 3.9 LA/ LNA ratio promoted highest expression of cyp19a1a (p<0.05). In conclusion, a 3.9 LA/ LNA ratio should be used for Nile tilapia female.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Ácidos graxos poli-insaturados (PUFA) e seus derivados de cadeia longa (LCPUFA) estão envolvidos em diversas funções no organismo. No entanto, um balanço adequado entre os ácidos graxos (AG) dietéticos provenientes de fontes vegetais, pode prevenir possível competição em suas rotas de biossíntese. Objetivou-se, neste trabalho, avaliar o efeito de diferentes relações dos ácidos graxos ácido linoleico/ linolênico (LA/LNA) nas dietas de fêmeas de tilápia Nilo (Oreochromis niloticus). Realizou-se um ensaio experimental, utilizando-se 24 machos e 72 fêmeas, com peso inicial médio de 131,3g e 124,4g, respectivamente. Seis fêmeas e dois machos foram distribuídos em doze tanques de 250L cada. O delineamento foi inteiramente casualisado, com 4 tratamentos (20,1; 4,5; 3,9 e 0,7 LA/ LNA) e três repetições. Diariamente, foram realizadas observações para a ocorrência de desova. As taxas de eclosão e tempo de sobrevivência das larvas foram registradas. No final do ensaio, amostras de sangue e tecidos foram coletados. Os peixes alimentados com as relações 3,9 e 0,7 tiveram maior número de ovos produzidos (p<0,05). A taxa de eclosão e a sobrevivência das larvas não foram afetadas pelas dietas (p>0,05). A taxa de crescimento específico foi maior na relação 0,7 e o índice hepatossomático, maior na relação 3,9 (p<0,05). De modo geral, os AG das gônadas refletiram a composição da dieta, sendo o teor de ácido araquidônico maior na relação 20,1 e os ácidos eicosapentaenoico (EPA) e docosahexaenoico (DHA), maiores na relação 0,7 (p<0,05). As dietas não influenciaram o teor de triglicerídeos plasmático (p>0,05), no entanto, as dietas com relações 3,9 e 4,5 proporcionaram maiores níveis de colesterol (p<0,05). As maiores concentrações de ácido linoleico dietético aumentaram a expressão gênica da enzima cyp17, enquanto a expressão da cyp19a1a foi maior na relação 4,5 (p<0,05). Conclui-se que uma relação dietética de 3,9 LA/LNA deve ser utilizada para fêmeas de tilápia do Nilo.Universidade Federal de LavrasPrograma de Pós-Graduação em ZootecniaUFLAbrasilDepartamento de ZootecniaRosa, Priscila VieiraOlmeda, Jose Fernando LopesMurgas, Luis David SolisAbreu, Márvio Lobão Teixeira deLuz, Ronald KennedyOrlando, Tamira Maria2018-04-19T13:20:53Z2018-04-19T13:20:53Z2018-04-182018-03-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfORLANDO, T. M. Effects of the ration of linoleic acid to linolenic acid dietary to tilapia females (Oreochromis niloticus). 2018. 71 p. Tese (Doutorado em Zootecnia)-Universidade Federal de Lavras, Lavras, 2018.http://repositorio.ufla.br/jspui/handle/1/29061enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLA2023-05-11T20:04:25Zoai:localhost:1/29061Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-05-11T20:04:25Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Effects of the ration of linoleic acid to linolenic acid dietary to tilapia females (Oreochromis niloticus) Efeitos da relação ácido linoleico/ ácido linolênico dietéticos para fêmeas de tilápia do Nilo (Oreochromisniloticus) |
title |
Effects of the ration of linoleic acid to linolenic acid dietary to tilapia females (Oreochromis niloticus) |
spellingShingle |
Effects of the ration of linoleic acid to linolenic acid dietary to tilapia females (Oreochromis niloticus) Orlando, Tamira Maria Ácidos graxos poli-insaturados Tilápia - Nutrição Lipídios Fatty acids Tilapia - Nutrition Lipids Zootecnia |
title_short |
Effects of the ration of linoleic acid to linolenic acid dietary to tilapia females (Oreochromis niloticus) |
title_full |
Effects of the ration of linoleic acid to linolenic acid dietary to tilapia females (Oreochromis niloticus) |
title_fullStr |
Effects of the ration of linoleic acid to linolenic acid dietary to tilapia females (Oreochromis niloticus) |
title_full_unstemmed |
Effects of the ration of linoleic acid to linolenic acid dietary to tilapia females (Oreochromis niloticus) |
title_sort |
Effects of the ration of linoleic acid to linolenic acid dietary to tilapia females (Oreochromis niloticus) |
author |
Orlando, Tamira Maria |
author_facet |
Orlando, Tamira Maria |
author_role |
author |
dc.contributor.none.fl_str_mv |
Rosa, Priscila Vieira Olmeda, Jose Fernando Lopes Murgas, Luis David Solis Abreu, Márvio Lobão Teixeira de Luz, Ronald Kennedy |
dc.contributor.author.fl_str_mv |
Orlando, Tamira Maria |
dc.subject.por.fl_str_mv |
Ácidos graxos poli-insaturados Tilápia - Nutrição Lipídios Fatty acids Tilapia - Nutrition Lipids Zootecnia |
topic |
Ácidos graxos poli-insaturados Tilápia - Nutrição Lipídios Fatty acids Tilapia - Nutrition Lipids Zootecnia |
description |
Polyunsaturated fatty acids (PUFAs) and their long chain derivatives (LCPUFA) are involved in various functions in the body. However, an adequate balance between dietary n-3 and n-6 fatty acids (FA) from plant sources, may prevent possible competition in their biosynthetic routes. The objective of this work, was to evaluate the effect of different dietary linoleic acid/ linolenic acid (LA/ LNA) ratio for Nile tilapia (Oreochromis niloticus) breeding females. An experiment was performed using 24 males and 72 females with mean initial weight of 131.3 g and 124.4 g, respectively. Six females and two males were distributed in twelve tanks of 250L each. The design was completely randomized, with four treatments (20.1, 4.5, 3.9 and 0.7 LA / LNA) and three replications. Observations were made for the occurrence of spawning every day. At the end of the test, blood and tissue samples were collected. Fish fed the 3.9 and 0.7 LA/LNA diets had higher number of eggs produced (p<0.05). Hatch rates and larval survival were not affected by the treatments (p>0.05). Specific growth rate was highest in fish fed the 0.7 LA/LNA ratio and hepatosomatic index was highest in fish fed the 4.5 LA/LNA ratio (p<0.05). In general, the FA profile of gonads reflected the composition of the diet. The arachidonic acid content was higher in 20.1 ratio and the eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids concentration were higher in 0.7 ratio (p<0,05). Diets did not influence the triglycerides content in plasma (p>0.05). However, higher levels of cholesterol were observed in fish fed 4.5 and 3.9 LA/ LNA ratio (p<0.05). The highest dietary LA levels increased the gene expression of cyp17, while the dietary 3.9 LA/ LNA ratio promoted highest expression of cyp19a1a (p<0.05). In conclusion, a 3.9 LA/ LNA ratio should be used for Nile tilapia female. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-04-19T13:20:53Z 2018-04-19T13:20:53Z 2018-04-18 2018-03-12 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
ORLANDO, T. M. Effects of the ration of linoleic acid to linolenic acid dietary to tilapia females (Oreochromis niloticus). 2018. 71 p. Tese (Doutorado em Zootecnia)-Universidade Federal de Lavras, Lavras, 2018. http://repositorio.ufla.br/jspui/handle/1/29061 |
identifier_str_mv |
ORLANDO, T. M. Effects of the ration of linoleic acid to linolenic acid dietary to tilapia females (Oreochromis niloticus). 2018. 71 p. Tese (Doutorado em Zootecnia)-Universidade Federal de Lavras, Lavras, 2018. |
url |
http://repositorio.ufla.br/jspui/handle/1/29061 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Lavras Programa de Pós-Graduação em Zootecnia UFLA brasil Departamento de Zootecnia |
publisher.none.fl_str_mv |
Universidade Federal de Lavras Programa de Pós-Graduação em Zootecnia UFLA brasil Departamento de Zootecnia |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
_version_ |
1807835122812059648 |