Dynamics of 45S rDNA sites in the cell cycle: fragile sites and chromosomal stability in Lolium and Festuca
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/32719 |
Resumo: | Analyses carried out with fluorescence in situ hybridization (FISH) in C-metaphases of the Lolium-Festuca complex have shown the occurrence of spontaneous fragile sites (FSs) in 45S rDNA regions. FSs are expressed as gaps but they do not result in breaks or chromosomal fragments in these species. These gaps have high DNA condensation observed as thin chromatin fibers that connect the apparent segments of the fragile chromosome, allowing for genomic stability. Assessing the behavior of these regions in the cell cycle of Lolium and Festuca species may lead to a better understanding of the dynamics that preserve stability during cell division. Furthermore, it is interesting to track the dynamics of chromosomes bearing 45S rDNA sites in the cell cycle as well as to observe the expression of FSs with no effect of the mitotic block. We observed variation in both the number and size of 45S FISH signals from the S/G2 phases of interphase and from prophase to anaphase where gaps in 45S rDNA sites also were observed. The change in the degree of condensation of the 45S site begins in the S/G2 phase and appears to be related to the transcriptional demand. Taking into account that the number of 45S rDNA sites tends to be reestablished when cells reach telophase, we suggest that the chromatin fiber goes back to the normal condensation level to the anaphase (after segregation), allowing for the approximation of chromosome segments and ensuring dynamics that favor the genomic stability of these species. |
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Dynamics of 45S rDNA sites in the cell cycle: fragile sites and chromosomal stability in Lolium and FestucaGenetic stabilityChromosomal fragmentsFragile siteGene activity-dependent decondensationChromatin fiberHigh decondensationAnalyses carried out with fluorescence in situ hybridization (FISH) in C-metaphases of the Lolium-Festuca complex have shown the occurrence of spontaneous fragile sites (FSs) in 45S rDNA regions. FSs are expressed as gaps but they do not result in breaks or chromosomal fragments in these species. These gaps have high DNA condensation observed as thin chromatin fibers that connect the apparent segments of the fragile chromosome, allowing for genomic stability. Assessing the behavior of these regions in the cell cycle of Lolium and Festuca species may lead to a better understanding of the dynamics that preserve stability during cell division. Furthermore, it is interesting to track the dynamics of chromosomes bearing 45S rDNA sites in the cell cycle as well as to observe the expression of FSs with no effect of the mitotic block. We observed variation in both the number and size of 45S FISH signals from the S/G2 phases of interphase and from prophase to anaphase where gaps in 45S rDNA sites also were observed. The change in the degree of condensation of the 45S site begins in the S/G2 phase and appears to be related to the transcriptional demand. Taking into account that the number of 45S rDNA sites tends to be reestablished when cells reach telophase, we suggest that the chromatin fiber goes back to the normal condensation level to the anaphase (after segregation), allowing for the approximation of chromosome segments and ensuring dynamics that favor the genomic stability of these species.Fundação de Pesquisas Científicas de Ribeirão Preto (FUNPEC-RP)2019-02-04T11:47:12Z2019-02-04T11:47:12Z2017-01-23info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfROCHA, L. C. et al. Dynamics of 45S rDNA sites in the cell cycle: fragile sites and chromosomal stability in Lolium and Festuca. Genetics and Molecular Research, Ribeirão Preto, v. 16, n. 1, p. 1-9, Jan. 2017.http://repositorio.ufla.br/jspui/handle/1/32719Genetics and Molecular Research (GMR)reponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAhttp://creativecommons.org/licenses/by-sa/4.0/info:eu-repo/semantics/openAccessRocha, L. C.Silva, G. A.Bustamante, F. O.Silveira, R. A. D.Mittlemann, A.Techio, V. H.eng2019-09-06T17:00:35Zoai:localhost:1/32719Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2019-09-06T17:00:35Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Dynamics of 45S rDNA sites in the cell cycle: fragile sites and chromosomal stability in Lolium and Festuca |
title |
Dynamics of 45S rDNA sites in the cell cycle: fragile sites and chromosomal stability in Lolium and Festuca |
spellingShingle |
Dynamics of 45S rDNA sites in the cell cycle: fragile sites and chromosomal stability in Lolium and Festuca Rocha, L. C. Genetic stability Chromosomal fragments Fragile site Gene activity-dependent decondensation Chromatin fiber High decondensation |
title_short |
Dynamics of 45S rDNA sites in the cell cycle: fragile sites and chromosomal stability in Lolium and Festuca |
title_full |
Dynamics of 45S rDNA sites in the cell cycle: fragile sites and chromosomal stability in Lolium and Festuca |
title_fullStr |
Dynamics of 45S rDNA sites in the cell cycle: fragile sites and chromosomal stability in Lolium and Festuca |
title_full_unstemmed |
Dynamics of 45S rDNA sites in the cell cycle: fragile sites and chromosomal stability in Lolium and Festuca |
title_sort |
Dynamics of 45S rDNA sites in the cell cycle: fragile sites and chromosomal stability in Lolium and Festuca |
author |
Rocha, L. C. |
author_facet |
Rocha, L. C. Silva, G. A. Bustamante, F. O. Silveira, R. A. D. Mittlemann, A. Techio, V. H. |
author_role |
author |
author2 |
Silva, G. A. Bustamante, F. O. Silveira, R. A. D. Mittlemann, A. Techio, V. H. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Rocha, L. C. Silva, G. A. Bustamante, F. O. Silveira, R. A. D. Mittlemann, A. Techio, V. H. |
dc.subject.por.fl_str_mv |
Genetic stability Chromosomal fragments Fragile site Gene activity-dependent decondensation Chromatin fiber High decondensation |
topic |
Genetic stability Chromosomal fragments Fragile site Gene activity-dependent decondensation Chromatin fiber High decondensation |
description |
Analyses carried out with fluorescence in situ hybridization (FISH) in C-metaphases of the Lolium-Festuca complex have shown the occurrence of spontaneous fragile sites (FSs) in 45S rDNA regions. FSs are expressed as gaps but they do not result in breaks or chromosomal fragments in these species. These gaps have high DNA condensation observed as thin chromatin fibers that connect the apparent segments of the fragile chromosome, allowing for genomic stability. Assessing the behavior of these regions in the cell cycle of Lolium and Festuca species may lead to a better understanding of the dynamics that preserve stability during cell division. Furthermore, it is interesting to track the dynamics of chromosomes bearing 45S rDNA sites in the cell cycle as well as to observe the expression of FSs with no effect of the mitotic block. We observed variation in both the number and size of 45S FISH signals from the S/G2 phases of interphase and from prophase to anaphase where gaps in 45S rDNA sites also were observed. The change in the degree of condensation of the 45S site begins in the S/G2 phase and appears to be related to the transcriptional demand. Taking into account that the number of 45S rDNA sites tends to be reestablished when cells reach telophase, we suggest that the chromatin fiber goes back to the normal condensation level to the anaphase (after segregation), allowing for the approximation of chromosome segments and ensuring dynamics that favor the genomic stability of these species. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-23 2019-02-04T11:47:12Z 2019-02-04T11:47:12Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
ROCHA, L. C. et al. Dynamics of 45S rDNA sites in the cell cycle: fragile sites and chromosomal stability in Lolium and Festuca. Genetics and Molecular Research, Ribeirão Preto, v. 16, n. 1, p. 1-9, Jan. 2017. http://repositorio.ufla.br/jspui/handle/1/32719 |
identifier_str_mv |
ROCHA, L. C. et al. Dynamics of 45S rDNA sites in the cell cycle: fragile sites and chromosomal stability in Lolium and Festuca. Genetics and Molecular Research, Ribeirão Preto, v. 16, n. 1, p. 1-9, Jan. 2017. |
url |
http://repositorio.ufla.br/jspui/handle/1/32719 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-sa/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-sa/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Fundação de Pesquisas Científicas de Ribeirão Preto (FUNPEC-RP) |
publisher.none.fl_str_mv |
Fundação de Pesquisas Científicas de Ribeirão Preto (FUNPEC-RP) |
dc.source.none.fl_str_mv |
Genetics and Molecular Research (GMR) reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
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1807835163683454976 |