Acclimatization and leaf anatomy of micropropagated fig plantlets
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/38256 |
Resumo: | The survival of micropropagated plants during and after acclimatization is a limiting process to plant establishment. There is little information on how the anatomy of vegetative organs of Ficus carica can be affected by culture conditions and acclimatization. The present research aimed to study the effects of time on culture medium and substrates during the acclimatization of fig tree plantlets produced in vitro, characterizing some leaf anatomy aspects of plantlets cultured in vitro and of fig trees produced in field. Plantlets previously multiplied in vitro were separated and transferred into Wood Plant Medium (WPM) where they were kept for 0, 15, 30, 45 and 60 days. Different substrates were tested and studies on leaf anatomy were performed in order to compare among plantlets grown in vitro, plantlets under 20, 40 and 60 days of acclimatization, and field grown plants. Keeping plantlets for 30 days in WPM allowed better development in Plantmax during acclimatization. Field grown plants presented higher number of stomata, greater epicuticular wax thickness and greater leaf tissue production compared to in vitro ones. The leaf tissues of in vitro plantlets show little differentiation and have great stomata number compared with acclimatized plants, which reduce the number of stomata during the acclimatization process. |
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Acclimatization and leaf anatomy of micropropagated fig plantletsAclimatização e anatomia foliar de plântulas de figo micropropagadasFicus carica L.SubstrateStomataParenchymaSubstratoEstômatoParênquimaThe survival of micropropagated plants during and after acclimatization is a limiting process to plant establishment. There is little information on how the anatomy of vegetative organs of Ficus carica can be affected by culture conditions and acclimatization. The present research aimed to study the effects of time on culture medium and substrates during the acclimatization of fig tree plantlets produced in vitro, characterizing some leaf anatomy aspects of plantlets cultured in vitro and of fig trees produced in field. Plantlets previously multiplied in vitro were separated and transferred into Wood Plant Medium (WPM) where they were kept for 0, 15, 30, 45 and 60 days. Different substrates were tested and studies on leaf anatomy were performed in order to compare among plantlets grown in vitro, plantlets under 20, 40 and 60 days of acclimatization, and field grown plants. Keeping plantlets for 30 days in WPM allowed better development in Plantmax during acclimatization. Field grown plants presented higher number of stomata, greater epicuticular wax thickness and greater leaf tissue production compared to in vitro ones. The leaf tissues of in vitro plantlets show little differentiation and have great stomata number compared with acclimatized plants, which reduce the number of stomata during the acclimatization process.A sobrevivência de plantas micropropagadas durante e após a aclimatização é um processo limitante para o estabelecimento de plantas. Poucas informações são encontradas de como a anatomia de órgãos vegetativos de Ficus carica pode ser afetada pelas condições de cultivo e aclimatização. O presente trabalho teve por objetivo estudar os efeitos do tempo em meio de cultura e substratos durante a aclimatização de plântulas de figueira produzidas in vitro e cultivadas no campo. Plântulas multiplicadas in vitro foram separadas e transferidas para meio WPM, onde foram mantidas por 0; 15; 30; 45 e 60 dias. Diferentes substratos foram testados, e os estudos de anatomia foliar foram executados a fim de comparar plântulas crescidas in vitro, plântulas com 20; 40 e 60 dias de aclimatização e plantas crescidas a campo. Plântulas mantidas por 30 dias em WPM apresentaram melhor desenvolvimento em Plantmax durante a aclimatização. Plantas de campo apresentam elevado número de estômatos, maior espessura de cera epicuticular e do tecido foliar em comparação com plantas cultivadas in vitro. Já plântulas crescidas in vitro mostram tecidos foliares pouco desenvolvidos e maior número de estômatos em comparação com plantas aclimatizadas, que reduzem o número de estômatos ao longo do processo de aclimatização.Sociedade Brasileira de Fruticultura (SBF)2019-12-17T14:09:43Z2019-12-17T14:09:43Z2012-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfCHIRINÉA, C. F. et al. Acclimatization and leaf anatomy of micropropagated fig plantlets. Revista Brasileira de Fruticultura, Jaboticabal, v. 34, n. 4, p. 1180-1188, Dez. 2012. DOI: 10.1590/S0100-29452012000400027.http://repositorio.ufla.br/jspui/handle/1/38256Revista Brasileira de Fruticultura (RBF)reponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution-NonCommercial 4.0 Internationalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessChirinéa, Chrystiane FráguasPasqual, MoacirAraujo, Aparecida Gomes dePereira, Alba ReginaCastro, Evaristo Mauro deeng2019-12-17T14:09:43Zoai:localhost:1/38256Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2019-12-17T14:09:43Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Acclimatization and leaf anatomy of micropropagated fig plantlets Aclimatização e anatomia foliar de plântulas de figo micropropagadas |
title |
Acclimatization and leaf anatomy of micropropagated fig plantlets |
spellingShingle |
Acclimatization and leaf anatomy of micropropagated fig plantlets Chirinéa, Chrystiane Fráguas Ficus carica L. Substrate Stomata Parenchyma Substrato Estômato Parênquima |
title_short |
Acclimatization and leaf anatomy of micropropagated fig plantlets |
title_full |
Acclimatization and leaf anatomy of micropropagated fig plantlets |
title_fullStr |
Acclimatization and leaf anatomy of micropropagated fig plantlets |
title_full_unstemmed |
Acclimatization and leaf anatomy of micropropagated fig plantlets |
title_sort |
Acclimatization and leaf anatomy of micropropagated fig plantlets |
author |
Chirinéa, Chrystiane Fráguas |
author_facet |
Chirinéa, Chrystiane Fráguas Pasqual, Moacir Araujo, Aparecida Gomes de Pereira, Alba Regina Castro, Evaristo Mauro de |
author_role |
author |
author2 |
Pasqual, Moacir Araujo, Aparecida Gomes de Pereira, Alba Regina Castro, Evaristo Mauro de |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Chirinéa, Chrystiane Fráguas Pasqual, Moacir Araujo, Aparecida Gomes de Pereira, Alba Regina Castro, Evaristo Mauro de |
dc.subject.por.fl_str_mv |
Ficus carica L. Substrate Stomata Parenchyma Substrato Estômato Parênquima |
topic |
Ficus carica L. Substrate Stomata Parenchyma Substrato Estômato Parênquima |
description |
The survival of micropropagated plants during and after acclimatization is a limiting process to plant establishment. There is little information on how the anatomy of vegetative organs of Ficus carica can be affected by culture conditions and acclimatization. The present research aimed to study the effects of time on culture medium and substrates during the acclimatization of fig tree plantlets produced in vitro, characterizing some leaf anatomy aspects of plantlets cultured in vitro and of fig trees produced in field. Plantlets previously multiplied in vitro were separated and transferred into Wood Plant Medium (WPM) where they were kept for 0, 15, 30, 45 and 60 days. Different substrates were tested and studies on leaf anatomy were performed in order to compare among plantlets grown in vitro, plantlets under 20, 40 and 60 days of acclimatization, and field grown plants. Keeping plantlets for 30 days in WPM allowed better development in Plantmax during acclimatization. Field grown plants presented higher number of stomata, greater epicuticular wax thickness and greater leaf tissue production compared to in vitro ones. The leaf tissues of in vitro plantlets show little differentiation and have great stomata number compared with acclimatized plants, which reduce the number of stomata during the acclimatization process. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-12 2019-12-17T14:09:43Z 2019-12-17T14:09:43Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
CHIRINÉA, C. F. et al. Acclimatization and leaf anatomy of micropropagated fig plantlets. Revista Brasileira de Fruticultura, Jaboticabal, v. 34, n. 4, p. 1180-1188, Dez. 2012. DOI: 10.1590/S0100-29452012000400027. http://repositorio.ufla.br/jspui/handle/1/38256 |
identifier_str_mv |
CHIRINÉA, C. F. et al. Acclimatization and leaf anatomy of micropropagated fig plantlets. Revista Brasileira de Fruticultura, Jaboticabal, v. 34, n. 4, p. 1180-1188, Dez. 2012. DOI: 10.1590/S0100-29452012000400027. |
url |
http://repositorio.ufla.br/jspui/handle/1/38256 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial 4.0 International http://creativecommons.org/licenses/by-nc/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial 4.0 International http://creativecommons.org/licenses/by-nc/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Fruticultura (SBF) |
publisher.none.fl_str_mv |
Sociedade Brasileira de Fruticultura (SBF) |
dc.source.none.fl_str_mv |
Revista Brasileira de Fruticultura (RBF) reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
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1807835086457929728 |