Inibição de enzimas digestivas por pellets de lúpulo (Humulus lupulus L.)
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/12551 |
Resumo: | The use of medicinal plants represents a wide field of research in the search for new therapeutic sources for the treatment of obesity, among other diseases. The objective of this study was to evaluate the inhibitory potential of the aqueous hop extract (in pellet form) on the digestive enzymes α-amylase, α-glucosidase, lipase and trypsin. An aqueous hop extract (in pellet form) was prepared in the 1:20 ratio (w/v) and used as an inhibitor in enzyme assays, which were performed in the absence and presence of simulated gastric fluid, in order to simulate the digestive process in the stomach in vitro. The effect of temperature on the activity of the inhibitors was also evaluated. In the absence of simulated gastric fluid, there was inhibition of enzymes α-amylase (80.91 ± 1.16%), α-glucosidase (40.96 ± 3.57%) and trypsin (67.48 ± 3.69%), whereas in the presence of simulated gastric fluid the inhibitory capacity of the extract disappeared for α-amylase, had a slight increase for α-glucosidase (44.25 ± 2.81%) and a significant increase for trypsin (89.01 ± 1.45%). Heat treatment did not alter the inhibitory capacity of the extract. We concluded that the aqueous hop extract has a potential use in the treatment of obesity. However, a more detailed study is still necessary for better understanding its mechanisms of action, which would thus enable future applications in the treatment of this pathology or for several therapeutic purposes. |
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Inibição de enzimas digestivas por pellets de lúpulo (Humulus lupulus L.)Inhibition of digestive enzymes by pellets of hop (Humulus lupulus L.)Plantas medicinaisObesidade - TratamentoEnzimas digestivasThe use of medicinal plants represents a wide field of research in the search for new therapeutic sources for the treatment of obesity, among other diseases. The objective of this study was to evaluate the inhibitory potential of the aqueous hop extract (in pellet form) on the digestive enzymes α-amylase, α-glucosidase, lipase and trypsin. An aqueous hop extract (in pellet form) was prepared in the 1:20 ratio (w/v) and used as an inhibitor in enzyme assays, which were performed in the absence and presence of simulated gastric fluid, in order to simulate the digestive process in the stomach in vitro. The effect of temperature on the activity of the inhibitors was also evaluated. In the absence of simulated gastric fluid, there was inhibition of enzymes α-amylase (80.91 ± 1.16%), α-glucosidase (40.96 ± 3.57%) and trypsin (67.48 ± 3.69%), whereas in the presence of simulated gastric fluid the inhibitory capacity of the extract disappeared for α-amylase, had a slight increase for α-glucosidase (44.25 ± 2.81%) and a significant increase for trypsin (89.01 ± 1.45%). Heat treatment did not alter the inhibitory capacity of the extract. We concluded that the aqueous hop extract has a potential use in the treatment of obesity. However, a more detailed study is still necessary for better understanding its mechanisms of action, which would thus enable future applications in the treatment of this pathology or for several therapeutic purposes.A utilização de plantas medicinais representa um vasto campo de pesquisa na busca de novas fontes terapêuticas para tratamento da obesidade, entre outras doen- ças. Objetivou-se neste trabalho avaliar o potencial inibitório do extrato aquoso de lúpulo (forma de pellets) sobre as enzimas digestivas α-amilase, α-glicosidase, lipase e tripsina. Foi preparado um extrato aquoso de lúpulo (forma de pellets) na proporção 1:20 (p/v) que foi utilizado como inibidor nos ensaios enzimáticos, que foram realizados na ausência e presença de fluido gástrico simulado afim de simular o processo de digestão no estômago in vitro. Avaliou-se também o efeito da temperatura na atividade dos inibidores. Na ausência do fluído gástrico simulado houve inibição das enzimas α-amilase (80,91 ± 1,16%), α-glicosidase (40,96 ± 3,57%) e tripsina (67,48 ± 3,69%), enquanto que na presença do fluído gástrico simulado, a capacidade inibitória do extrato desapareceu para a α-amilase, teve pequeno aumento para a α-glicosidase (44,25 ± 2,81%) e aumento significativo para a tripsina (89,01 ± 1,45%). O tratamento térmico não alterou a capacidade inibitória do extrato. Conclui-se que o extrato aquoso de lúpulo apresenta potencial no tratamento da obesidade, porém, um estudo mais detalhado ainda se faz necessário para a melhor compreensão de seus mecanismos de ação, possibilitando futuras aplicações no tratamento desta patologia ou para diversos fins terapêuticos.Universidade Federal do Rio Grande do Sul2017-03-24T11:06:07Z2017-03-24T11:06:07Z2014-10info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfMARQUES, T. R. et al. Inibição de enzimas digestivas por pellets de lúpulo (Humulus lupulus L.). Revista Brasileira de Biociências, Porto Alegre, v. 12, n. 4, p. 183-187, out. 2014.http://repositorio.ufla.br/jspui/handle/1/12551Revista Brasileira de Biociênciasreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAMarques, Tamara RezendePereira, LucianaSimão, AndersonRamos, ViniciusBraga, MarianaCorrêa, AngelitaSantos, Custódioinfo:eu-repo/semantics/openAccesspor2017-03-24T11:06:07Zoai:localhost:1/12551Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2017-03-24T11:06:07Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Inibição de enzimas digestivas por pellets de lúpulo (Humulus lupulus L.) Inhibition of digestive enzymes by pellets of hop (Humulus lupulus L.) |
title |
Inibição de enzimas digestivas por pellets de lúpulo (Humulus lupulus L.) |
spellingShingle |
Inibição de enzimas digestivas por pellets de lúpulo (Humulus lupulus L.) Marques, Tamara Rezende Plantas medicinais Obesidade - Tratamento Enzimas digestivas |
title_short |
Inibição de enzimas digestivas por pellets de lúpulo (Humulus lupulus L.) |
title_full |
Inibição de enzimas digestivas por pellets de lúpulo (Humulus lupulus L.) |
title_fullStr |
Inibição de enzimas digestivas por pellets de lúpulo (Humulus lupulus L.) |
title_full_unstemmed |
Inibição de enzimas digestivas por pellets de lúpulo (Humulus lupulus L.) |
title_sort |
Inibição de enzimas digestivas por pellets de lúpulo (Humulus lupulus L.) |
author |
Marques, Tamara Rezende |
author_facet |
Marques, Tamara Rezende Pereira, Luciana Simão, Anderson Ramos, Vinicius Braga, Mariana Corrêa, Angelita Santos, Custódio |
author_role |
author |
author2 |
Pereira, Luciana Simão, Anderson Ramos, Vinicius Braga, Mariana Corrêa, Angelita Santos, Custódio |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Marques, Tamara Rezende Pereira, Luciana Simão, Anderson Ramos, Vinicius Braga, Mariana Corrêa, Angelita Santos, Custódio |
dc.subject.por.fl_str_mv |
Plantas medicinais Obesidade - Tratamento Enzimas digestivas |
topic |
Plantas medicinais Obesidade - Tratamento Enzimas digestivas |
description |
The use of medicinal plants represents a wide field of research in the search for new therapeutic sources for the treatment of obesity, among other diseases. The objective of this study was to evaluate the inhibitory potential of the aqueous hop extract (in pellet form) on the digestive enzymes α-amylase, α-glucosidase, lipase and trypsin. An aqueous hop extract (in pellet form) was prepared in the 1:20 ratio (w/v) and used as an inhibitor in enzyme assays, which were performed in the absence and presence of simulated gastric fluid, in order to simulate the digestive process in the stomach in vitro. The effect of temperature on the activity of the inhibitors was also evaluated. In the absence of simulated gastric fluid, there was inhibition of enzymes α-amylase (80.91 ± 1.16%), α-glucosidase (40.96 ± 3.57%) and trypsin (67.48 ± 3.69%), whereas in the presence of simulated gastric fluid the inhibitory capacity of the extract disappeared for α-amylase, had a slight increase for α-glucosidase (44.25 ± 2.81%) and a significant increase for trypsin (89.01 ± 1.45%). Heat treatment did not alter the inhibitory capacity of the extract. We concluded that the aqueous hop extract has a potential use in the treatment of obesity. However, a more detailed study is still necessary for better understanding its mechanisms of action, which would thus enable future applications in the treatment of this pathology or for several therapeutic purposes. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-10 2017-03-24T11:06:07Z 2017-03-24T11:06:07Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
MARQUES, T. R. et al. Inibição de enzimas digestivas por pellets de lúpulo (Humulus lupulus L.). Revista Brasileira de Biociências, Porto Alegre, v. 12, n. 4, p. 183-187, out. 2014. http://repositorio.ufla.br/jspui/handle/1/12551 |
identifier_str_mv |
MARQUES, T. R. et al. Inibição de enzimas digestivas por pellets de lúpulo (Humulus lupulus L.). Revista Brasileira de Biociências, Porto Alegre, v. 12, n. 4, p. 183-187, out. 2014. |
url |
http://repositorio.ufla.br/jspui/handle/1/12551 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal do Rio Grande do Sul |
publisher.none.fl_str_mv |
Universidade Federal do Rio Grande do Sul |
dc.source.none.fl_str_mv |
Revista Brasileira de Biociências reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
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UFLA |
reponame_str |
Repositório Institucional da UFLA |
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Repositório Institucional da UFLA |
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Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
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nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
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1807835197290315776 |