Genetic similarity among strawberry cultivars assessed by RAPD and ISSR markers
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/37579 |
Resumo: | Most strawberry (Fragaria × ananassa Duchesne) cultivars used in Brazil are developed in other countries, it became clear the need to start the strawberry breeding program in the country. To start a breeding program is necessary the genetic characterization of the germplasm available. Molecular markers are important tools that can be used for this purpose. The objectives of the present study were to assess the genetic similarity among 11 strawberry cultivars using RAPD and ISSR molecular markers and to indicate the possible promising crosses. The DNA of the eleven strawberry cultivars was extracted and amplified by PCR with RAPD and ISSR primers. The DNA fragments were separated in agarose gel for the RAPD markers and in polyacrylamide gel for the ISSR markers. The genetic similarity matrix was estimated by the Jaccard coefficient. Based on this matrix, the cultivars were grouped using the UPGMA method. The dendogram generated by the RAPD markers distributed the cultivars in three groups while the ISSR markers generated two groups. There was no direct relationship between the marker groups when the two types of markers were compared. The grouping proposed by the ISSR markers was more coherent with the origin and the genealogy of the cultivars than that proposed by the RAPD markers, and it can be considered the most efficient method for the study of genetic divergence in strawberry. The most promising crosses, based on the genetic divergence estimated from the RAPD and ISSR molecular data were between the Tudla and Ventana and the Oso Grande and Ventana cultivars, respectively. |
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Genetic similarity among strawberry cultivars assessed by RAPD and ISSR markersFragaria × ananassaPlant breedingGenetic divergenceMolecular markersMost strawberry (Fragaria × ananassa Duchesne) cultivars used in Brazil are developed in other countries, it became clear the need to start the strawberry breeding program in the country. To start a breeding program is necessary the genetic characterization of the germplasm available. Molecular markers are important tools that can be used for this purpose. The objectives of the present study were to assess the genetic similarity among 11 strawberry cultivars using RAPD and ISSR molecular markers and to indicate the possible promising crosses. The DNA of the eleven strawberry cultivars was extracted and amplified by PCR with RAPD and ISSR primers. The DNA fragments were separated in agarose gel for the RAPD markers and in polyacrylamide gel for the ISSR markers. The genetic similarity matrix was estimated by the Jaccard coefficient. Based on this matrix, the cultivars were grouped using the UPGMA method. The dendogram generated by the RAPD markers distributed the cultivars in three groups while the ISSR markers generated two groups. There was no direct relationship between the marker groups when the two types of markers were compared. The grouping proposed by the ISSR markers was more coherent with the origin and the genealogy of the cultivars than that proposed by the RAPD markers, and it can be considered the most efficient method for the study of genetic divergence in strawberry. The most promising crosses, based on the genetic divergence estimated from the RAPD and ISSR molecular data were between the Tudla and Ventana and the Oso Grande and Ventana cultivars, respectively.Universidade de São Paulo (USP), Escola Superior de Agricultura "Luiz de Queiroz" (ESALQ)2019-11-08T12:39:52Z2019-11-08T12:39:52Z2011-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfMORALES, R. G. F. et al. Genetic similarity among strawberry cultivars assessed by RAPD and ISSR markers. Scientia Agrícola, Piracicaba, v. 68, n. 6, p. 665-670, Nov./Dec. 2011. DOI: 10.1590/S0103-90162011000600010.http://repositorio.ufla.br/jspui/handle/1/37579Scientia Agrícolareponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution-NonCommercial 4.0 Internationalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessMorales, Rafael Gustavo FerreiraResende, Juliano Tadeu VilelaFaria, Marcos VenturaAndrade, Marcela CarvalhoResende, Luciane VilelaDelatorre, Carla AndreaSilva, Paulo Roberto daeng2023-05-26T18:50:17Zoai:localhost:1/37579Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-05-26T18:50:17Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Genetic similarity among strawberry cultivars assessed by RAPD and ISSR markers |
title |
Genetic similarity among strawberry cultivars assessed by RAPD and ISSR markers |
spellingShingle |
Genetic similarity among strawberry cultivars assessed by RAPD and ISSR markers Morales, Rafael Gustavo Ferreira Fragaria × ananassa Plant breeding Genetic divergence Molecular markers |
title_short |
Genetic similarity among strawberry cultivars assessed by RAPD and ISSR markers |
title_full |
Genetic similarity among strawberry cultivars assessed by RAPD and ISSR markers |
title_fullStr |
Genetic similarity among strawberry cultivars assessed by RAPD and ISSR markers |
title_full_unstemmed |
Genetic similarity among strawberry cultivars assessed by RAPD and ISSR markers |
title_sort |
Genetic similarity among strawberry cultivars assessed by RAPD and ISSR markers |
author |
Morales, Rafael Gustavo Ferreira |
author_facet |
Morales, Rafael Gustavo Ferreira Resende, Juliano Tadeu Vilela Faria, Marcos Ventura Andrade, Marcela Carvalho Resende, Luciane Vilela Delatorre, Carla Andrea Silva, Paulo Roberto da |
author_role |
author |
author2 |
Resende, Juliano Tadeu Vilela Faria, Marcos Ventura Andrade, Marcela Carvalho Resende, Luciane Vilela Delatorre, Carla Andrea Silva, Paulo Roberto da |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Morales, Rafael Gustavo Ferreira Resende, Juliano Tadeu Vilela Faria, Marcos Ventura Andrade, Marcela Carvalho Resende, Luciane Vilela Delatorre, Carla Andrea Silva, Paulo Roberto da |
dc.subject.por.fl_str_mv |
Fragaria × ananassa Plant breeding Genetic divergence Molecular markers |
topic |
Fragaria × ananassa Plant breeding Genetic divergence Molecular markers |
description |
Most strawberry (Fragaria × ananassa Duchesne) cultivars used in Brazil are developed in other countries, it became clear the need to start the strawberry breeding program in the country. To start a breeding program is necessary the genetic characterization of the germplasm available. Molecular markers are important tools that can be used for this purpose. The objectives of the present study were to assess the genetic similarity among 11 strawberry cultivars using RAPD and ISSR molecular markers and to indicate the possible promising crosses. The DNA of the eleven strawberry cultivars was extracted and amplified by PCR with RAPD and ISSR primers. The DNA fragments were separated in agarose gel for the RAPD markers and in polyacrylamide gel for the ISSR markers. The genetic similarity matrix was estimated by the Jaccard coefficient. Based on this matrix, the cultivars were grouped using the UPGMA method. The dendogram generated by the RAPD markers distributed the cultivars in three groups while the ISSR markers generated two groups. There was no direct relationship between the marker groups when the two types of markers were compared. The grouping proposed by the ISSR markers was more coherent with the origin and the genealogy of the cultivars than that proposed by the RAPD markers, and it can be considered the most efficient method for the study of genetic divergence in strawberry. The most promising crosses, based on the genetic divergence estimated from the RAPD and ISSR molecular data were between the Tudla and Ventana and the Oso Grande and Ventana cultivars, respectively. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-12 2019-11-08T12:39:52Z 2019-11-08T12:39:52Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
MORALES, R. G. F. et al. Genetic similarity among strawberry cultivars assessed by RAPD and ISSR markers. Scientia Agrícola, Piracicaba, v. 68, n. 6, p. 665-670, Nov./Dec. 2011. DOI: 10.1590/S0103-90162011000600010. http://repositorio.ufla.br/jspui/handle/1/37579 |
identifier_str_mv |
MORALES, R. G. F. et al. Genetic similarity among strawberry cultivars assessed by RAPD and ISSR markers. Scientia Agrícola, Piracicaba, v. 68, n. 6, p. 665-670, Nov./Dec. 2011. DOI: 10.1590/S0103-90162011000600010. |
url |
http://repositorio.ufla.br/jspui/handle/1/37579 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial 4.0 International http://creativecommons.org/licenses/by-nc/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial 4.0 International http://creativecommons.org/licenses/by-nc/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo (USP), Escola Superior de Agricultura "Luiz de Queiroz" (ESALQ) |
publisher.none.fl_str_mv |
Universidade de São Paulo (USP), Escola Superior de Agricultura "Luiz de Queiroz" (ESALQ) |
dc.source.none.fl_str_mv |
Scientia Agrícola reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
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1807835187484033024 |