Morphometric analysis on chromosomes of tropical pinus species

Detalhes bibliográficos
Autor(a) principal: Ribeiro, Lília Rosário
Data de Publicação: 2009
Outros Autores: Davide, Lisete Chamma, Pedrozo, Cássia Ângela, Barbosa, Sandro, Torres, Giovana Augusta
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/37364
Resumo: Karyotypic analysis of Pinus oocarpa Schiede ex Schltdl., Pinus patula Schltdl. & Cham. and of provenances Jócon Yoro, Las Camelias and San Rafael del Norte of Pinus tecunumanii Eguiluz & J. P. Perry were accomplished to provide information for definition of Pinus tecunumanii taxonomic status. Characterization of mitotic chromosomes stained with Giemsa and with 4’, 6-diamidino-2- phenylindoldihydrochlorid (DAPI) fluorochrome confirmed the karyotypic pattern reported for most of the Pinus species, which present eleven pairs of metacentric chromosomes, very similar in regard to length and morphology, and a submetacentric pair for all the taxa studied. Sharp definition of secondary constrictions revealed by DAPI staining allowed distinction of species and provenances by the number and position of this cytological marker. Pinus tecunumanii was also different from the other two species in regard to total length of haploid set. The results support the species status for Pinus tecunumanii as well as present evidence that in addition to point mutations, structural alterations contributed toward intra and interspecies differentiation into the genus Pinus.
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spelling Morphometric analysis on chromosomes of tropical pinus speciesAnálise morfométrica em cromossomos de espécies tropicais de PinusCariótipoConstrição secundáriaPinusCitotaxonomiaKaryotypeSecondary constrictionCytotaxonomyKaryotypic analysis of Pinus oocarpa Schiede ex Schltdl., Pinus patula Schltdl. & Cham. and of provenances Jócon Yoro, Las Camelias and San Rafael del Norte of Pinus tecunumanii Eguiluz & J. P. Perry were accomplished to provide information for definition of Pinus tecunumanii taxonomic status. Characterization of mitotic chromosomes stained with Giemsa and with 4’, 6-diamidino-2- phenylindoldihydrochlorid (DAPI) fluorochrome confirmed the karyotypic pattern reported for most of the Pinus species, which present eleven pairs of metacentric chromosomes, very similar in regard to length and morphology, and a submetacentric pair for all the taxa studied. Sharp definition of secondary constrictions revealed by DAPI staining allowed distinction of species and provenances by the number and position of this cytological marker. Pinus tecunumanii was also different from the other two species in regard to total length of haploid set. The results support the species status for Pinus tecunumanii as well as present evidence that in addition to point mutations, structural alterations contributed toward intra and interspecies differentiation into the genus Pinus.Foram realizados estudos citogenéticos em Pinus oocarpa Schiede ex Schltdl., Pinus patula Schltdl. & Cham. e nas procedências Jócon Yoro, Las Camelias e San Rafael del Norte do Pinus tecunumanii Eguiluz & J. P. Perry, visando subsidiar a definição da categoria taxonômica do Pinus tecunumanii. A caracterização dos cromossomos mitóticos, utilizando coloração com Giemsa e com o fluorocromo 4’, 6- diamidino-2-fenilindoldiidroclorídrico (DAPI), confirmou o padrão cariotípico descrito para a maioria das espécies do gênero, isto é, onze pares de cromossomos metacêntricos, muito semelhantes quanto ao comprimento e morfologia, e um par submetacêntrico, para todos os taxa estudados. A coloração com o fluorocromo forneceu definição clara das constrições secundárias, permitindo a diferenciação das espécies e procedências pelo número e posição das mesmas. As procedências de Pinus tecunumanii também se diferenciaram dos outros dois materiais em relação ao comprimento total do complemento haplóide. Os resultados obtidos suportam o “status” específico para o Pinus tecunumanii, bem como fornecem evidências de que além das mutações de ponto, alterações estruturais contribuíram para a diferenciação intra e interespecífica no gênero Pinus.Universidade Federal de Santa Maria2019-10-29T13:16:28Z2019-10-29T13:16:28Z2009info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfRIBEIRO, L. R. et al. Morphometric analysis on chromosomes of tropical pinus species. Ciência Florestal, Santa Maria, v. 19, n. 1, p. 83-90, Jan./mar. 2009.http://repositorio.ufla.br/jspui/handle/1/37364Ciência Florestalreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution-NonCommercial 4.0 Internationalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessRibeiro, Lília RosárioDavide, Lisete ChammaPedrozo, Cássia ÂngelaBarbosa, SandroTorres, Giovana Augustaeng2019-10-29T13:16:58Zoai:localhost:1/37364Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2019-10-29T13:16:58Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Morphometric analysis on chromosomes of tropical pinus species
Análise morfométrica em cromossomos de espécies tropicais de Pinus
title Morphometric analysis on chromosomes of tropical pinus species
spellingShingle Morphometric analysis on chromosomes of tropical pinus species
Ribeiro, Lília Rosário
Cariótipo
Constrição secundária
Pinus
Citotaxonomia
Karyotype
Secondary constriction
Cytotaxonomy
title_short Morphometric analysis on chromosomes of tropical pinus species
title_full Morphometric analysis on chromosomes of tropical pinus species
title_fullStr Morphometric analysis on chromosomes of tropical pinus species
title_full_unstemmed Morphometric analysis on chromosomes of tropical pinus species
title_sort Morphometric analysis on chromosomes of tropical pinus species
author Ribeiro, Lília Rosário
author_facet Ribeiro, Lília Rosário
Davide, Lisete Chamma
Pedrozo, Cássia Ângela
Barbosa, Sandro
Torres, Giovana Augusta
author_role author
author2 Davide, Lisete Chamma
Pedrozo, Cássia Ângela
Barbosa, Sandro
Torres, Giovana Augusta
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Ribeiro, Lília Rosário
Davide, Lisete Chamma
Pedrozo, Cássia Ângela
Barbosa, Sandro
Torres, Giovana Augusta
dc.subject.por.fl_str_mv Cariótipo
Constrição secundária
Pinus
Citotaxonomia
Karyotype
Secondary constriction
Cytotaxonomy
topic Cariótipo
Constrição secundária
Pinus
Citotaxonomia
Karyotype
Secondary constriction
Cytotaxonomy
description Karyotypic analysis of Pinus oocarpa Schiede ex Schltdl., Pinus patula Schltdl. & Cham. and of provenances Jócon Yoro, Las Camelias and San Rafael del Norte of Pinus tecunumanii Eguiluz & J. P. Perry were accomplished to provide information for definition of Pinus tecunumanii taxonomic status. Characterization of mitotic chromosomes stained with Giemsa and with 4’, 6-diamidino-2- phenylindoldihydrochlorid (DAPI) fluorochrome confirmed the karyotypic pattern reported for most of the Pinus species, which present eleven pairs of metacentric chromosomes, very similar in regard to length and morphology, and a submetacentric pair for all the taxa studied. Sharp definition of secondary constrictions revealed by DAPI staining allowed distinction of species and provenances by the number and position of this cytological marker. Pinus tecunumanii was also different from the other two species in regard to total length of haploid set. The results support the species status for Pinus tecunumanii as well as present evidence that in addition to point mutations, structural alterations contributed toward intra and interspecies differentiation into the genus Pinus.
publishDate 2009
dc.date.none.fl_str_mv 2009
2019-10-29T13:16:28Z
2019-10-29T13:16:28Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv RIBEIRO, L. R. et al. Morphometric analysis on chromosomes of tropical pinus species. Ciência Florestal, Santa Maria, v. 19, n. 1, p. 83-90, Jan./mar. 2009.
http://repositorio.ufla.br/jspui/handle/1/37364
identifier_str_mv RIBEIRO, L. R. et al. Morphometric analysis on chromosomes of tropical pinus species. Ciência Florestal, Santa Maria, v. 19, n. 1, p. 83-90, Jan./mar. 2009.
url http://repositorio.ufla.br/jspui/handle/1/37364
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Ciência Florestal
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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