Morphometric analysis on chromosomes of tropical pinus species
Autor(a) principal: | |
---|---|
Data de Publicação: | 2009 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/37364 |
Resumo: | Karyotypic analysis of Pinus oocarpa Schiede ex Schltdl., Pinus patula Schltdl. & Cham. and of provenances Jócon Yoro, Las Camelias and San Rafael del Norte of Pinus tecunumanii Eguiluz & J. P. Perry were accomplished to provide information for definition of Pinus tecunumanii taxonomic status. Characterization of mitotic chromosomes stained with Giemsa and with 4’, 6-diamidino-2- phenylindoldihydrochlorid (DAPI) fluorochrome confirmed the karyotypic pattern reported for most of the Pinus species, which present eleven pairs of metacentric chromosomes, very similar in regard to length and morphology, and a submetacentric pair for all the taxa studied. Sharp definition of secondary constrictions revealed by DAPI staining allowed distinction of species and provenances by the number and position of this cytological marker. Pinus tecunumanii was also different from the other two species in regard to total length of haploid set. The results support the species status for Pinus tecunumanii as well as present evidence that in addition to point mutations, structural alterations contributed toward intra and interspecies differentiation into the genus Pinus. |
id |
UFLA_8bd6820fa4d24d8a9bebfa83a9d8207c |
---|---|
oai_identifier_str |
oai:localhost:1/37364 |
network_acronym_str |
UFLA |
network_name_str |
Repositório Institucional da UFLA |
repository_id_str |
|
spelling |
Morphometric analysis on chromosomes of tropical pinus speciesAnálise morfométrica em cromossomos de espécies tropicais de PinusCariótipoConstrição secundáriaPinusCitotaxonomiaKaryotypeSecondary constrictionCytotaxonomyKaryotypic analysis of Pinus oocarpa Schiede ex Schltdl., Pinus patula Schltdl. & Cham. and of provenances Jócon Yoro, Las Camelias and San Rafael del Norte of Pinus tecunumanii Eguiluz & J. P. Perry were accomplished to provide information for definition of Pinus tecunumanii taxonomic status. Characterization of mitotic chromosomes stained with Giemsa and with 4’, 6-diamidino-2- phenylindoldihydrochlorid (DAPI) fluorochrome confirmed the karyotypic pattern reported for most of the Pinus species, which present eleven pairs of metacentric chromosomes, very similar in regard to length and morphology, and a submetacentric pair for all the taxa studied. Sharp definition of secondary constrictions revealed by DAPI staining allowed distinction of species and provenances by the number and position of this cytological marker. Pinus tecunumanii was also different from the other two species in regard to total length of haploid set. The results support the species status for Pinus tecunumanii as well as present evidence that in addition to point mutations, structural alterations contributed toward intra and interspecies differentiation into the genus Pinus.Foram realizados estudos citogenéticos em Pinus oocarpa Schiede ex Schltdl., Pinus patula Schltdl. & Cham. e nas procedências Jócon Yoro, Las Camelias e San Rafael del Norte do Pinus tecunumanii Eguiluz & J. P. Perry, visando subsidiar a definição da categoria taxonômica do Pinus tecunumanii. A caracterização dos cromossomos mitóticos, utilizando coloração com Giemsa e com o fluorocromo 4’, 6- diamidino-2-fenilindoldiidroclorídrico (DAPI), confirmou o padrão cariotípico descrito para a maioria das espécies do gênero, isto é, onze pares de cromossomos metacêntricos, muito semelhantes quanto ao comprimento e morfologia, e um par submetacêntrico, para todos os taxa estudados. A coloração com o fluorocromo forneceu definição clara das constrições secundárias, permitindo a diferenciação das espécies e procedências pelo número e posição das mesmas. As procedências de Pinus tecunumanii também se diferenciaram dos outros dois materiais em relação ao comprimento total do complemento haplóide. Os resultados obtidos suportam o “status” específico para o Pinus tecunumanii, bem como fornecem evidências de que além das mutações de ponto, alterações estruturais contribuíram para a diferenciação intra e interespecífica no gênero Pinus.Universidade Federal de Santa Maria2019-10-29T13:16:28Z2019-10-29T13:16:28Z2009info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfRIBEIRO, L. R. et al. Morphometric analysis on chromosomes of tropical pinus species. Ciência Florestal, Santa Maria, v. 19, n. 1, p. 83-90, Jan./mar. 2009.http://repositorio.ufla.br/jspui/handle/1/37364Ciência Florestalreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution-NonCommercial 4.0 Internationalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessRibeiro, Lília RosárioDavide, Lisete ChammaPedrozo, Cássia ÂngelaBarbosa, SandroTorres, Giovana Augustaeng2019-10-29T13:16:58Zoai:localhost:1/37364Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2019-10-29T13:16:58Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Morphometric analysis on chromosomes of tropical pinus species Análise morfométrica em cromossomos de espécies tropicais de Pinus |
title |
Morphometric analysis on chromosomes of tropical pinus species |
spellingShingle |
Morphometric analysis on chromosomes of tropical pinus species Ribeiro, Lília Rosário Cariótipo Constrição secundária Pinus Citotaxonomia Karyotype Secondary constriction Cytotaxonomy |
title_short |
Morphometric analysis on chromosomes of tropical pinus species |
title_full |
Morphometric analysis on chromosomes of tropical pinus species |
title_fullStr |
Morphometric analysis on chromosomes of tropical pinus species |
title_full_unstemmed |
Morphometric analysis on chromosomes of tropical pinus species |
title_sort |
Morphometric analysis on chromosomes of tropical pinus species |
author |
Ribeiro, Lília Rosário |
author_facet |
Ribeiro, Lília Rosário Davide, Lisete Chamma Pedrozo, Cássia Ângela Barbosa, Sandro Torres, Giovana Augusta |
author_role |
author |
author2 |
Davide, Lisete Chamma Pedrozo, Cássia Ângela Barbosa, Sandro Torres, Giovana Augusta |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Ribeiro, Lília Rosário Davide, Lisete Chamma Pedrozo, Cássia Ângela Barbosa, Sandro Torres, Giovana Augusta |
dc.subject.por.fl_str_mv |
Cariótipo Constrição secundária Pinus Citotaxonomia Karyotype Secondary constriction Cytotaxonomy |
topic |
Cariótipo Constrição secundária Pinus Citotaxonomia Karyotype Secondary constriction Cytotaxonomy |
description |
Karyotypic analysis of Pinus oocarpa Schiede ex Schltdl., Pinus patula Schltdl. & Cham. and of provenances Jócon Yoro, Las Camelias and San Rafael del Norte of Pinus tecunumanii Eguiluz & J. P. Perry were accomplished to provide information for definition of Pinus tecunumanii taxonomic status. Characterization of mitotic chromosomes stained with Giemsa and with 4’, 6-diamidino-2- phenylindoldihydrochlorid (DAPI) fluorochrome confirmed the karyotypic pattern reported for most of the Pinus species, which present eleven pairs of metacentric chromosomes, very similar in regard to length and morphology, and a submetacentric pair for all the taxa studied. Sharp definition of secondary constrictions revealed by DAPI staining allowed distinction of species and provenances by the number and position of this cytological marker. Pinus tecunumanii was also different from the other two species in regard to total length of haploid set. The results support the species status for Pinus tecunumanii as well as present evidence that in addition to point mutations, structural alterations contributed toward intra and interspecies differentiation into the genus Pinus. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009 2019-10-29T13:16:28Z 2019-10-29T13:16:28Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
RIBEIRO, L. R. et al. Morphometric analysis on chromosomes of tropical pinus species. Ciência Florestal, Santa Maria, v. 19, n. 1, p. 83-90, Jan./mar. 2009. http://repositorio.ufla.br/jspui/handle/1/37364 |
identifier_str_mv |
RIBEIRO, L. R. et al. Morphometric analysis on chromosomes of tropical pinus species. Ciência Florestal, Santa Maria, v. 19, n. 1, p. 83-90, Jan./mar. 2009. |
url |
http://repositorio.ufla.br/jspui/handle/1/37364 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial 4.0 International http://creativecommons.org/licenses/by-nc/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial 4.0 International http://creativecommons.org/licenses/by-nc/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Florestal reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
_version_ |
1807835142129975296 |