Use of sex-sorted and unsorted frozen/thawed sperm and in vitro fertilization events in bovine oocytes derived from ultrasound-guided aspiration

Detalhes bibliográficos
Autor(a) principal: López, Salvador Ruiz
Data de Publicação: 2013
Outros Autores: Souza, José Camisão de, González, Javier Zaraza, Sánchez, Aitor de Ondiz, Romero-Aguirregomezcorta, Jon, Carvalho, Rafaela Rodrigues de, Rath, Detlef
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/38294
Resumo: The objective was to investigate the effects of sex-sorting on early fertilization events in ovum pick up (OPU) derived oocytes fertilized in vitro with frozen-thawed sperm at different co-incubation lengths. Eighty-four OPU sessions were carried out in 18 cyclic, dry and non-stimulated Holstein Friesian and German black pied cows. Ovum pick up oocytes were matured in vitro for 24 hours and fertilized with frozen-thawed sex sorted or unsorted sperm from the same ejaculate. Fertilization was achieved by two experimental protocols: 1) short gamete co-incubation length: 4, 8 and 12 hours; and 2) long gamete co-incubation length: 18 and 24 hours. After in vitro fertilization, ova were fixed and stained to identify early fertilization events. Sperm penetration, monospermy, pronuclear formation and syngamy did not differ, whether sexed or unsexed sperm was used. Overall, the findings demonstrate similar fertilizing potential between sex-sorted and unsorted sperm.
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spelling Use of sex-sorted and unsorted frozen/thawed sperm and in vitro fertilization events in bovine oocytes derived from ultrasound-guided aspirationCo-incubation lengthEarly fertilizationOvum pick upSex-sorted spermThe objective was to investigate the effects of sex-sorting on early fertilization events in ovum pick up (OPU) derived oocytes fertilized in vitro with frozen-thawed sperm at different co-incubation lengths. Eighty-four OPU sessions were carried out in 18 cyclic, dry and non-stimulated Holstein Friesian and German black pied cows. Ovum pick up oocytes were matured in vitro for 24 hours and fertilized with frozen-thawed sex sorted or unsorted sperm from the same ejaculate. Fertilization was achieved by two experimental protocols: 1) short gamete co-incubation length: 4, 8 and 12 hours; and 2) long gamete co-incubation length: 18 and 24 hours. After in vitro fertilization, ova were fixed and stained to identify early fertilization events. Sperm penetration, monospermy, pronuclear formation and syngamy did not differ, whether sexed or unsexed sperm was used. Overall, the findings demonstrate similar fertilizing potential between sex-sorted and unsorted sperm.Sociedade Brasileira de Zootecnia2019-12-19T16:15:05Z2019-12-19T16:15:05Z2013info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfLÓPEZ, S. R. et al. Use of sex-sorted and unsorted frozen/thawed sperm and in vitro fertilization events in bovine oocytes derived from ultrasound-guided aspiration. Revista Brasileira de Zootecnia, [S.l.], v. 42, n. 10, p. 721-727, 2013.http://repositorio.ufla.br/jspui/handle/1/38294Revista Brasileira de Zootecniareponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution-NonCommercial 4.0 Internationalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessLópez, Salvador RuizSouza, José Camisão deGonzález, Javier ZarazaSánchez, Aitor de OndizRomero-Aguirregomezcorta, JonCarvalho, Rafaela Rodrigues deRath, Detlefeng2019-12-19T16:15:40Zoai:localhost:1/38294Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2019-12-19T16:15:40Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Use of sex-sorted and unsorted frozen/thawed sperm and in vitro fertilization events in bovine oocytes derived from ultrasound-guided aspiration
title Use of sex-sorted and unsorted frozen/thawed sperm and in vitro fertilization events in bovine oocytes derived from ultrasound-guided aspiration
spellingShingle Use of sex-sorted and unsorted frozen/thawed sperm and in vitro fertilization events in bovine oocytes derived from ultrasound-guided aspiration
López, Salvador Ruiz
Co-incubation length
Early fertilization
Ovum pick up
Sex-sorted sperm
title_short Use of sex-sorted and unsorted frozen/thawed sperm and in vitro fertilization events in bovine oocytes derived from ultrasound-guided aspiration
title_full Use of sex-sorted and unsorted frozen/thawed sperm and in vitro fertilization events in bovine oocytes derived from ultrasound-guided aspiration
title_fullStr Use of sex-sorted and unsorted frozen/thawed sperm and in vitro fertilization events in bovine oocytes derived from ultrasound-guided aspiration
title_full_unstemmed Use of sex-sorted and unsorted frozen/thawed sperm and in vitro fertilization events in bovine oocytes derived from ultrasound-guided aspiration
title_sort Use of sex-sorted and unsorted frozen/thawed sperm and in vitro fertilization events in bovine oocytes derived from ultrasound-guided aspiration
author López, Salvador Ruiz
author_facet López, Salvador Ruiz
Souza, José Camisão de
González, Javier Zaraza
Sánchez, Aitor de Ondiz
Romero-Aguirregomezcorta, Jon
Carvalho, Rafaela Rodrigues de
Rath, Detlef
author_role author
author2 Souza, José Camisão de
González, Javier Zaraza
Sánchez, Aitor de Ondiz
Romero-Aguirregomezcorta, Jon
Carvalho, Rafaela Rodrigues de
Rath, Detlef
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv López, Salvador Ruiz
Souza, José Camisão de
González, Javier Zaraza
Sánchez, Aitor de Ondiz
Romero-Aguirregomezcorta, Jon
Carvalho, Rafaela Rodrigues de
Rath, Detlef
dc.subject.por.fl_str_mv Co-incubation length
Early fertilization
Ovum pick up
Sex-sorted sperm
topic Co-incubation length
Early fertilization
Ovum pick up
Sex-sorted sperm
description The objective was to investigate the effects of sex-sorting on early fertilization events in ovum pick up (OPU) derived oocytes fertilized in vitro with frozen-thawed sperm at different co-incubation lengths. Eighty-four OPU sessions were carried out in 18 cyclic, dry and non-stimulated Holstein Friesian and German black pied cows. Ovum pick up oocytes were matured in vitro for 24 hours and fertilized with frozen-thawed sex sorted or unsorted sperm from the same ejaculate. Fertilization was achieved by two experimental protocols: 1) short gamete co-incubation length: 4, 8 and 12 hours; and 2) long gamete co-incubation length: 18 and 24 hours. After in vitro fertilization, ova were fixed and stained to identify early fertilization events. Sperm penetration, monospermy, pronuclear formation and syngamy did not differ, whether sexed or unsexed sperm was used. Overall, the findings demonstrate similar fertilizing potential between sex-sorted and unsorted sperm.
publishDate 2013
dc.date.none.fl_str_mv 2013
2019-12-19T16:15:05Z
2019-12-19T16:15:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv LÓPEZ, S. R. et al. Use of sex-sorted and unsorted frozen/thawed sperm and in vitro fertilization events in bovine oocytes derived from ultrasound-guided aspiration. Revista Brasileira de Zootecnia, [S.l.], v. 42, n. 10, p. 721-727, 2013.
http://repositorio.ufla.br/jspui/handle/1/38294
identifier_str_mv LÓPEZ, S. R. et al. Use of sex-sorted and unsorted frozen/thawed sperm and in vitro fertilization events in bovine oocytes derived from ultrasound-guided aspiration. Revista Brasileira de Zootecnia, [S.l.], v. 42, n. 10, p. 721-727, 2013.
url http://repositorio.ufla.br/jspui/handle/1/38294
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Zootecnia
publisher.none.fl_str_mv Sociedade Brasileira de Zootecnia
dc.source.none.fl_str_mv Revista Brasileira de Zootecnia
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
_version_ 1807835164605153280

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