Minimum inhibitory concentration of essential oils against Staphylococcus aureus isolated from dogs with external otitis
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/49103 |
Resumo: | Canine otitis externa is a disease that affects the external acoustic meatus of about 20% of dogs at some point in life, without predilection for race, age, or sex. It is a multifactorial disease whose etiology involves several microorganisms, detaching Staphylococcus aureus. Antimicrobials are the basis for treating this illness; however, due to the increase in antimicrobial resistance, conventional drugs have become ineffective, requiring the search for alternative therapies. In this context, essential oils (EOs) have great therapeutic potential due to their broad antimicrobial action. This study aimed to evaluate the minimum inhibitory concentration (MIC) in order to measure the MIC50 and MIC90 of gentamicin and EOs of Eugenia caryophyllata, Thymus vulgaris, Cymbopogon winterianus, Cymbopogon citratus, and Cinnamomum cassia against 62 Staphylococcus aureus strains isolated from the external acoustic meatus of dogs. All EOs showed antibacterial action against the studied microorganisms, and their MIC50 and MIC90 were as follows: Eugenia caryophyllata, 2.42 mg mL-1 and 7.25 mg mL-1; Thymus vulgaris, 9.51 mg mL-1 and 22.94 mg mL-1; Cymbopogon winterianus, 26.78 mg mL-1 and 157.79 mg mL-1; Cinnamomum cassia and Cymbopogon citratus, lower than 16.48 and 27.81 mg mL-1, with the same MIC for all isolates. The MIC50 and MIC90 found for gentamicin were 1μg mL-1 and 8 μg mL-1. The MIC range found to antibiotic in this assay was 0.5 μg mL-1 to 128 μg mL-1, and the isolates were classified as susceptible [48 strains (77.41%) - MIC range of 0.5-4.0 μg mL-1], intermediate [eight strains (12.90%) - (MIC = 8.0 μg mL-1], or resistant [six strains (9.68%) - MIC ≥ 16 μg mL-1]. The results, according to the in vitro assays, showed that resistance to gentamicin, one of the antimicrobials most commonly used to treat canine otitis, is present in the Staphylococcus aureus population evaluated. Additionally, the tested EOs have great potential for therapeutic use, however future studies should be carried out to evaluate their in vivo efficacy. |
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Minimum inhibitory concentration of essential oils against Staphylococcus aureus isolated from dogs with external otitisConcentração inibitória mínima de óleos essenciais contra Staphylococcus aureus isolados de cães com otite externaPhytotherapyOtopathiesAntibacterialsNatural antimicrobialsFitoterapiaOtopatiasAntibacterianosAntimicrobianos naturaisCanine otitis externa is a disease that affects the external acoustic meatus of about 20% of dogs at some point in life, without predilection for race, age, or sex. It is a multifactorial disease whose etiology involves several microorganisms, detaching Staphylococcus aureus. Antimicrobials are the basis for treating this illness; however, due to the increase in antimicrobial resistance, conventional drugs have become ineffective, requiring the search for alternative therapies. In this context, essential oils (EOs) have great therapeutic potential due to their broad antimicrobial action. This study aimed to evaluate the minimum inhibitory concentration (MIC) in order to measure the MIC50 and MIC90 of gentamicin and EOs of Eugenia caryophyllata, Thymus vulgaris, Cymbopogon winterianus, Cymbopogon citratus, and Cinnamomum cassia against 62 Staphylococcus aureus strains isolated from the external acoustic meatus of dogs. All EOs showed antibacterial action against the studied microorganisms, and their MIC50 and MIC90 were as follows: Eugenia caryophyllata, 2.42 mg mL-1 and 7.25 mg mL-1; Thymus vulgaris, 9.51 mg mL-1 and 22.94 mg mL-1; Cymbopogon winterianus, 26.78 mg mL-1 and 157.79 mg mL-1; Cinnamomum cassia and Cymbopogon citratus, lower than 16.48 and 27.81 mg mL-1, with the same MIC for all isolates. The MIC50 and MIC90 found for gentamicin were 1μg mL-1 and 8 μg mL-1. The MIC range found to antibiotic in this assay was 0.5 μg mL-1 to 128 μg mL-1, and the isolates were classified as susceptible [48 strains (77.41%) - MIC range of 0.5-4.0 μg mL-1], intermediate [eight strains (12.90%) - (MIC = 8.0 μg mL-1], or resistant [six strains (9.68%) - MIC ≥ 16 μg mL-1]. The results, according to the in vitro assays, showed that resistance to gentamicin, one of the antimicrobials most commonly used to treat canine otitis, is present in the Staphylococcus aureus population evaluated. Additionally, the tested EOs have great potential for therapeutic use, however future studies should be carried out to evaluate their in vivo efficacy.A otite canina externa é uma doença que afeta o meato acústico externo de até 20% dos cães em algum período de suas vidas, sem predileção por raça, idade ou sexo. Trata-se de uma doença multifatorial que tem Staphylococcus aureus como um dos principais agentes etiológicos. Antimicrobianos constituem a base para o tratamento desta enfermidade, entretanto, devido ao incremento da resistência antimicrobiana, os fármacos convencionais tem se tornado pouco eficazes, o que requer a busca por terapias alternativas. Neste contexto, os óleos essenciais (OE) apresentam grande potencial terapêutico devido a sua ampla ação antimicrobiana. Este trabalho visou detectar a concentração inibitória mínima (CIM), a partir da qual foram obtidos os índices de MIC50 e MIC90 para a gentamicina e para os OE de Eugenia caryophyllata, Thymus vulgaris, Cymbopogon winterianus, Cymbopogon citratus e Cinnamomum cassia contra 62 Staphylococcus aureus isolados do meato acústico externo de cães otopatas. Todos os OE mostraram ação antibacteriana contra os microrganismos estudados e foram detectadas as seguintes CIM50 e CIM90: Eugenia caryophyllata, 2,42 mg mL-1 e 7,45 mg mL-1; Thymus vulgaris, 9,51 mg mL-1 e 22,94 mg mL-1; Cymbopogon winterianus, 26,78 mg mL-1 e 157,79 mg mL-1; Cinnamomum cassia e Cymbopogon citratus com CIM menores que 16,48 e 27,81 mg mL-1, respectivamente já que estes apresentaram CIM única para todos os isolados testados. Para a gentamicina, foram obtidas CIM50 e CIM90 1μg mL-1 e 8 μg/m, respectivamente. Ademais, a faixa de CIM encontrada para o antibiótico variou de 0,5 a 128 μg mL-1, e os isolados foram classificados em susceptíveis [48 isolados (77,41%) - CIM na faixa de 0,5-4 μg mL-1], intermediários [8 isolados (12,90%) - (CIM = 8μg mL-1] e resistentes [6 isolados (9,68%) - CIM ≥16]. Os resultados encontrados mostraram que os micro-organismos testados tem potencial para desenvolver resistência antimicrobiana a terapêutica tradicional e que os OE, de acordo com os resultados in vitro, apresentam potencial de uso terapêutico, entretanto futuros estudos devem ser realizados para avaliar a eficácia dos OE in vivo.Universidade Estadual de Londrina2022-01-31T18:09:17Z2022-01-31T18:09:17Z2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfCOSTA, G. M. et al. Minimum inhibitory concentration of essential oils against Staphylococcus aureus isolated from dogs with external otitis. Semina: Ciências Agrárias, Londrina, v. 42, n. 6, 2021.http://repositorio.ufla.br/jspui/handle/1/49103Semina: Ciências Agráriasreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution-NonCommercial 4.0 Internationalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessCosta, Geraldo MárcioPrado, Ingrid AlexandreCarvalho-Castro, Glei dos Anjos deMian, Glaúcia FrasnelliLeite, Carlos Artur LopesCustódio, Dircéia Aparecida CostaPiccoli, Roberta Hilsdorfeng2022-01-31T18:09:17Zoai:localhost:1/49103Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2022-01-31T18:09:17Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Minimum inhibitory concentration of essential oils against Staphylococcus aureus isolated from dogs with external otitis Concentração inibitória mínima de óleos essenciais contra Staphylococcus aureus isolados de cães com otite externa |
title |
Minimum inhibitory concentration of essential oils against Staphylococcus aureus isolated from dogs with external otitis |
spellingShingle |
Minimum inhibitory concentration of essential oils against Staphylococcus aureus isolated from dogs with external otitis Costa, Geraldo Márcio Phytotherapy Otopathies Antibacterials Natural antimicrobials Fitoterapia Otopatias Antibacterianos Antimicrobianos naturais |
title_short |
Minimum inhibitory concentration of essential oils against Staphylococcus aureus isolated from dogs with external otitis |
title_full |
Minimum inhibitory concentration of essential oils against Staphylococcus aureus isolated from dogs with external otitis |
title_fullStr |
Minimum inhibitory concentration of essential oils against Staphylococcus aureus isolated from dogs with external otitis |
title_full_unstemmed |
Minimum inhibitory concentration of essential oils against Staphylococcus aureus isolated from dogs with external otitis |
title_sort |
Minimum inhibitory concentration of essential oils against Staphylococcus aureus isolated from dogs with external otitis |
author |
Costa, Geraldo Márcio |
author_facet |
Costa, Geraldo Márcio Prado, Ingrid Alexandre Carvalho-Castro, Glei dos Anjos de Mian, Glaúcia Frasnelli Leite, Carlos Artur Lopes Custódio, Dircéia Aparecida Costa Piccoli, Roberta Hilsdorf |
author_role |
author |
author2 |
Prado, Ingrid Alexandre Carvalho-Castro, Glei dos Anjos de Mian, Glaúcia Frasnelli Leite, Carlos Artur Lopes Custódio, Dircéia Aparecida Costa Piccoli, Roberta Hilsdorf |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Costa, Geraldo Márcio Prado, Ingrid Alexandre Carvalho-Castro, Glei dos Anjos de Mian, Glaúcia Frasnelli Leite, Carlos Artur Lopes Custódio, Dircéia Aparecida Costa Piccoli, Roberta Hilsdorf |
dc.subject.por.fl_str_mv |
Phytotherapy Otopathies Antibacterials Natural antimicrobials Fitoterapia Otopatias Antibacterianos Antimicrobianos naturais |
topic |
Phytotherapy Otopathies Antibacterials Natural antimicrobials Fitoterapia Otopatias Antibacterianos Antimicrobianos naturais |
description |
Canine otitis externa is a disease that affects the external acoustic meatus of about 20% of dogs at some point in life, without predilection for race, age, or sex. It is a multifactorial disease whose etiology involves several microorganisms, detaching Staphylococcus aureus. Antimicrobials are the basis for treating this illness; however, due to the increase in antimicrobial resistance, conventional drugs have become ineffective, requiring the search for alternative therapies. In this context, essential oils (EOs) have great therapeutic potential due to their broad antimicrobial action. This study aimed to evaluate the minimum inhibitory concentration (MIC) in order to measure the MIC50 and MIC90 of gentamicin and EOs of Eugenia caryophyllata, Thymus vulgaris, Cymbopogon winterianus, Cymbopogon citratus, and Cinnamomum cassia against 62 Staphylococcus aureus strains isolated from the external acoustic meatus of dogs. All EOs showed antibacterial action against the studied microorganisms, and their MIC50 and MIC90 were as follows: Eugenia caryophyllata, 2.42 mg mL-1 and 7.25 mg mL-1; Thymus vulgaris, 9.51 mg mL-1 and 22.94 mg mL-1; Cymbopogon winterianus, 26.78 mg mL-1 and 157.79 mg mL-1; Cinnamomum cassia and Cymbopogon citratus, lower than 16.48 and 27.81 mg mL-1, with the same MIC for all isolates. The MIC50 and MIC90 found for gentamicin were 1μg mL-1 and 8 μg mL-1. The MIC range found to antibiotic in this assay was 0.5 μg mL-1 to 128 μg mL-1, and the isolates were classified as susceptible [48 strains (77.41%) - MIC range of 0.5-4.0 μg mL-1], intermediate [eight strains (12.90%) - (MIC = 8.0 μg mL-1], or resistant [six strains (9.68%) - MIC ≥ 16 μg mL-1]. The results, according to the in vitro assays, showed that resistance to gentamicin, one of the antimicrobials most commonly used to treat canine otitis, is present in the Staphylococcus aureus population evaluated. Additionally, the tested EOs have great potential for therapeutic use, however future studies should be carried out to evaluate their in vivo efficacy. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021 2022-01-31T18:09:17Z 2022-01-31T18:09:17Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
COSTA, G. M. et al. Minimum inhibitory concentration of essential oils against Staphylococcus aureus isolated from dogs with external otitis. Semina: Ciências Agrárias, Londrina, v. 42, n. 6, 2021. http://repositorio.ufla.br/jspui/handle/1/49103 |
identifier_str_mv |
COSTA, G. M. et al. Minimum inhibitory concentration of essential oils against Staphylococcus aureus isolated from dogs with external otitis. Semina: Ciências Agrárias, Londrina, v. 42, n. 6, 2021. |
url |
http://repositorio.ufla.br/jspui/handle/1/49103 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial 4.0 International http://creativecommons.org/licenses/by-nc/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial 4.0 International http://creativecommons.org/licenses/by-nc/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Londrina |
publisher.none.fl_str_mv |
Universidade Estadual de Londrina |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
_version_ |
1807835097136627712 |