Light quality in micropropagation of Eucalyptus grandis × Eucalyptus urophylla
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/46910 |
Resumo: | The importance of eucalypts species and their interspecific hybrids has been evidenced in forestry programmes due to their wood quality and adaptation to diverse environmental conditions. To solve the limiting factors in the in vitro cultivation of the A211 clone of Eucalyptus grandis Hill ex Maiden × Eucalyptus urophylla S. T. Blake, the aim of the study was to evaluate the light quality on in vitro establishment, multiplication, elongation and rooting stages. The tissues used to obtain explants were nodal segments derived from ministumps of the A211 clone. The effects of light quality on in vitro establishment, multiplication, elongation and rooting were evaluated using five sources (fluorescent lamp, white LEDs, red LEDs, blue LEDs and red/blue LEDs). At 30 days, the percentage of contamination and non-responsive explants, length and number of shoots per explant, oxidation, shoot vigor, pigment content, leaf anatomy, root length and number of roots per explant were evaluated. Fluorescent lamps and red/blue LEDs are more suitable for use in the in vitro establishment, multiplication, elongation and rooting of the A211 clone, without hindering the development of shoots for the production of micropropagated clonal plants. |
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Light quality in micropropagation of Eucalyptus grandis × Eucalyptus urophyllaQualidade de luz na micropropagação de Eucalytpus grandis × Eucalyptus urophyllaIn vitro propagationWave-lengthLEDsCloningPropagação in vitroComprimento de ondaLEDsEucalipto - ClonagemThe importance of eucalypts species and their interspecific hybrids has been evidenced in forestry programmes due to their wood quality and adaptation to diverse environmental conditions. To solve the limiting factors in the in vitro cultivation of the A211 clone of Eucalyptus grandis Hill ex Maiden × Eucalyptus urophylla S. T. Blake, the aim of the study was to evaluate the light quality on in vitro establishment, multiplication, elongation and rooting stages. The tissues used to obtain explants were nodal segments derived from ministumps of the A211 clone. The effects of light quality on in vitro establishment, multiplication, elongation and rooting were evaluated using five sources (fluorescent lamp, white LEDs, red LEDs, blue LEDs and red/blue LEDs). At 30 days, the percentage of contamination and non-responsive explants, length and number of shoots per explant, oxidation, shoot vigor, pigment content, leaf anatomy, root length and number of roots per explant were evaluated. Fluorescent lamps and red/blue LEDs are more suitable for use in the in vitro establishment, multiplication, elongation and rooting of the A211 clone, without hindering the development of shoots for the production of micropropagated clonal plants.A importância das espécies de eucalipto e de seus híbridos interespecíficos tem sido evidenciada nos programas de silvicultura, devido à qualidade da madeira e adaptação a diversas condições ambientais. Para solucionar os fatores limitantes ao cultivo in vitro do clone A211 de Eucalyptus grandis Hill ex Maiden × Eucalyptus urophylla S. T. Blake, o presente trabalho teve como objetivo avaliar a influência da qualidade de luz nas fases de introdução, multiplicação, alongamento e enraizamento in vitro. Os tecidos utilizados para a obtenção dos explantes foram segmentos nodais, provenientes de minicepas do clone A211 de E. grandis × E. urophylla. Os efeitos no cultivo in vitro foram avaliados por meio de cinco fontes de qualidade de luz (Lâmpada fluorescente, LEDs branco, LEDs vermelho, LEDs azul e LEDs vermelho/azul). Dados de porcentagem de contaminação e explantes não responsivos, comprimento e número de brotos por explante, oxidação, vigor das brotações, conteúdo de pigmentos, anatomia foliar, comprimento e número de raízes por explante foram coletados aos 30 dias de cultivo in vitro. Com base nos resultados obtidos, pôde-se inferir que as lâmpadas fluorescentes e LEDs vermelho/azul são as mais adequadas para serem utilizadas na introdução, multiplicação, alongamento e enraizamento in vitro do clone A211 de E. grandis × E. urophylla, não ocasionando prejuízos no desenvolvimento de brotações destinadas à produção de plantas clonais micropropagadas.Instituto de Pesquisas e Estudos Florestais - IPEF2021-08-20T19:02:54Z2021-08-20T19:02:54Z2020-09info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfSOUZA, D. M. S. C. et al. Light quality in micropropagation of Eucalyptus grandis × Eucalyptus urophylla. Scientia Forestalis, Piracicaba, v. 48, n. 127, e3329, set. 2020. DOI: https://doi.org/10.18671/scifor.v48n127.03.http://repositorio.ufla.br/jspui/handle/1/46910Scientia Forestalisreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessSouza, Denys Matheus Santana CostaFernandes, Sérgio BrunoAvelar, Maria Lopes MartinsFrade, Samira Rangel do PradoMolinar, Letícia VazGonçalves, Douglas SantosPinto, José Eduardo Brasil PereiraBrondani, Gilvano Eblingeng2023-05-26T18:58:05Zoai:localhost:1/46910Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-05-26T18:58:05Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Light quality in micropropagation of Eucalyptus grandis × Eucalyptus urophylla Qualidade de luz na micropropagação de Eucalytpus grandis × Eucalyptus urophylla |
title |
Light quality in micropropagation of Eucalyptus grandis × Eucalyptus urophylla |
spellingShingle |
Light quality in micropropagation of Eucalyptus grandis × Eucalyptus urophylla Souza, Denys Matheus Santana Costa In vitro propagation Wave-length LEDs Cloning Propagação in vitro Comprimento de onda LEDs Eucalipto - Clonagem |
title_short |
Light quality in micropropagation of Eucalyptus grandis × Eucalyptus urophylla |
title_full |
Light quality in micropropagation of Eucalyptus grandis × Eucalyptus urophylla |
title_fullStr |
Light quality in micropropagation of Eucalyptus grandis × Eucalyptus urophylla |
title_full_unstemmed |
Light quality in micropropagation of Eucalyptus grandis × Eucalyptus urophylla |
title_sort |
Light quality in micropropagation of Eucalyptus grandis × Eucalyptus urophylla |
author |
Souza, Denys Matheus Santana Costa |
author_facet |
Souza, Denys Matheus Santana Costa Fernandes, Sérgio Bruno Avelar, Maria Lopes Martins Frade, Samira Rangel do Prado Molinar, Letícia Vaz Gonçalves, Douglas Santos Pinto, José Eduardo Brasil Pereira Brondani, Gilvano Ebling |
author_role |
author |
author2 |
Fernandes, Sérgio Bruno Avelar, Maria Lopes Martins Frade, Samira Rangel do Prado Molinar, Letícia Vaz Gonçalves, Douglas Santos Pinto, José Eduardo Brasil Pereira Brondani, Gilvano Ebling |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Souza, Denys Matheus Santana Costa Fernandes, Sérgio Bruno Avelar, Maria Lopes Martins Frade, Samira Rangel do Prado Molinar, Letícia Vaz Gonçalves, Douglas Santos Pinto, José Eduardo Brasil Pereira Brondani, Gilvano Ebling |
dc.subject.por.fl_str_mv |
In vitro propagation Wave-length LEDs Cloning Propagação in vitro Comprimento de onda LEDs Eucalipto - Clonagem |
topic |
In vitro propagation Wave-length LEDs Cloning Propagação in vitro Comprimento de onda LEDs Eucalipto - Clonagem |
description |
The importance of eucalypts species and their interspecific hybrids has been evidenced in forestry programmes due to their wood quality and adaptation to diverse environmental conditions. To solve the limiting factors in the in vitro cultivation of the A211 clone of Eucalyptus grandis Hill ex Maiden × Eucalyptus urophylla S. T. Blake, the aim of the study was to evaluate the light quality on in vitro establishment, multiplication, elongation and rooting stages. The tissues used to obtain explants were nodal segments derived from ministumps of the A211 clone. The effects of light quality on in vitro establishment, multiplication, elongation and rooting were evaluated using five sources (fluorescent lamp, white LEDs, red LEDs, blue LEDs and red/blue LEDs). At 30 days, the percentage of contamination and non-responsive explants, length and number of shoots per explant, oxidation, shoot vigor, pigment content, leaf anatomy, root length and number of roots per explant were evaluated. Fluorescent lamps and red/blue LEDs are more suitable for use in the in vitro establishment, multiplication, elongation and rooting of the A211 clone, without hindering the development of shoots for the production of micropropagated clonal plants. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-09 2021-08-20T19:02:54Z 2021-08-20T19:02:54Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
SOUZA, D. M. S. C. et al. Light quality in micropropagation of Eucalyptus grandis × Eucalyptus urophylla. Scientia Forestalis, Piracicaba, v. 48, n. 127, e3329, set. 2020. DOI: https://doi.org/10.18671/scifor.v48n127.03. http://repositorio.ufla.br/jspui/handle/1/46910 |
identifier_str_mv |
SOUZA, D. M. S. C. et al. Light quality in micropropagation of Eucalyptus grandis × Eucalyptus urophylla. Scientia Forestalis, Piracicaba, v. 48, n. 127, e3329, set. 2020. DOI: https://doi.org/10.18671/scifor.v48n127.03. |
url |
http://repositorio.ufla.br/jspui/handle/1/46910 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Instituto de Pesquisas e Estudos Florestais - IPEF |
publisher.none.fl_str_mv |
Instituto de Pesquisas e Estudos Florestais - IPEF |
dc.source.none.fl_str_mv |
Scientia Forestalis reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
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1807835125199667200 |