In vitro multiplication of Eucalyptus hybrid via temporary immersion bioreactor: culture media and cytokinin effects

Detalhes bibliográficos
Autor(a) principal: Máximo, Wesley Pires Flausino
Data de Publicação: 2018
Outros Autores: Santos, Paulo Augusto Almeida, Martins, Guilherme Silva, Mendonça, Evânia Galvão, Paiva, Luciano Vilela
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/34267
Resumo: This study aimed to assess the influence of culture medium and cytokinin type on in vitro multiplication of an Eucalyptus grandis x E. urophylla hybrid clone via temporary immersion bioreactor (TIB®). JADS, modified MS at a (NO3ˉ):(NH +) ratio, and WPM were used as liquid media; and 6-benzilaminopurine (BAP), kinetin (KIN), and thidiazuron (TDZ) were the cytokinins used in the study. According to the results, explants under the influence of modified MS presented better results for all traits analyzed in this study. BAP was the most suitable plant growth regulator for axillary bud shoot proliferation. By the leaf histological analysis, most of the shoots grown in the presence of BAP either exhibited normal morphology or only a few hyperhydricity symptoms. All these results were obtained at 19 days after in vitro cultivation, a shorter period when compared with other works in the literature, showing the protocol efficiency in the multiplication of this hybrid clone using TIB®.
id UFLA_b8eb3a85dbce16d187a8d6113f5f4fae
oai_identifier_str oai:localhost:1/34267
network_acronym_str UFLA
network_name_str Repositório Institucional da UFLA
repository_id_str
spelling In vitro multiplication of Eucalyptus hybrid via temporary immersion bioreactor: culture media and cytokinin effects6-BenzilaminopurineHyperhydricityPlant biotechnologyIn vitro propagationThis study aimed to assess the influence of culture medium and cytokinin type on in vitro multiplication of an Eucalyptus grandis x E. urophylla hybrid clone via temporary immersion bioreactor (TIB®). JADS, modified MS at a (NO3ˉ):(NH +) ratio, and WPM were used as liquid media; and 6-benzilaminopurine (BAP), kinetin (KIN), and thidiazuron (TDZ) were the cytokinins used in the study. According to the results, explants under the influence of modified MS presented better results for all traits analyzed in this study. BAP was the most suitable plant growth regulator for axillary bud shoot proliferation. By the leaf histological analysis, most of the shoots grown in the presence of BAP either exhibited normal morphology or only a few hyperhydricity symptoms. All these results were obtained at 19 days after in vitro cultivation, a shorter period when compared with other works in the literature, showing the protocol efficiency in the multiplication of this hybrid clone using TIB®.Crop Breeding and Applied Biotechnology2019-05-14T12:17:25Z2019-05-14T12:17:25Z2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfMÁXIMO, W. P. F. et al. In vitro multiplication of Eucalyptus hybrid via temporary immersion bioreactor: culture media and cytokinin effects. Crop Breeding and Applied Biotechnology, Viçosa, MG, v. 18, n. 2, Apr./June 2018.http://repositorio.ufla.br/jspui/handle/1/34267Crop Breeding and Applied Biotechnologyreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessMáximo, Wesley Pires FlausinoSantos, Paulo Augusto AlmeidaMartins, Guilherme SilvaMendonça, Evânia GalvãoPaiva, Luciano Vilelaeng2019-05-14T12:17:26Zoai:localhost:1/34267Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2019-05-14T12:17:26Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv In vitro multiplication of Eucalyptus hybrid via temporary immersion bioreactor: culture media and cytokinin effects
title In vitro multiplication of Eucalyptus hybrid via temporary immersion bioreactor: culture media and cytokinin effects
spellingShingle In vitro multiplication of Eucalyptus hybrid via temporary immersion bioreactor: culture media and cytokinin effects
Máximo, Wesley Pires Flausino
6-Benzilaminopurine
Hyperhydricity
Plant biotechnology
In vitro propagation
title_short In vitro multiplication of Eucalyptus hybrid via temporary immersion bioreactor: culture media and cytokinin effects
title_full In vitro multiplication of Eucalyptus hybrid via temporary immersion bioreactor: culture media and cytokinin effects
title_fullStr In vitro multiplication of Eucalyptus hybrid via temporary immersion bioreactor: culture media and cytokinin effects
title_full_unstemmed In vitro multiplication of Eucalyptus hybrid via temporary immersion bioreactor: culture media and cytokinin effects
title_sort In vitro multiplication of Eucalyptus hybrid via temporary immersion bioreactor: culture media and cytokinin effects
author Máximo, Wesley Pires Flausino
author_facet Máximo, Wesley Pires Flausino
Santos, Paulo Augusto Almeida
Martins, Guilherme Silva
Mendonça, Evânia Galvão
Paiva, Luciano Vilela
author_role author
author2 Santos, Paulo Augusto Almeida
Martins, Guilherme Silva
Mendonça, Evânia Galvão
Paiva, Luciano Vilela
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Máximo, Wesley Pires Flausino
Santos, Paulo Augusto Almeida
Martins, Guilherme Silva
Mendonça, Evânia Galvão
Paiva, Luciano Vilela
dc.subject.por.fl_str_mv 6-Benzilaminopurine
Hyperhydricity
Plant biotechnology
In vitro propagation
topic 6-Benzilaminopurine
Hyperhydricity
Plant biotechnology
In vitro propagation
description This study aimed to assess the influence of culture medium and cytokinin type on in vitro multiplication of an Eucalyptus grandis x E. urophylla hybrid clone via temporary immersion bioreactor (TIB®). JADS, modified MS at a (NO3ˉ):(NH +) ratio, and WPM were used as liquid media; and 6-benzilaminopurine (BAP), kinetin (KIN), and thidiazuron (TDZ) were the cytokinins used in the study. According to the results, explants under the influence of modified MS presented better results for all traits analyzed in this study. BAP was the most suitable plant growth regulator for axillary bud shoot proliferation. By the leaf histological analysis, most of the shoots grown in the presence of BAP either exhibited normal morphology or only a few hyperhydricity symptoms. All these results were obtained at 19 days after in vitro cultivation, a shorter period when compared with other works in the literature, showing the protocol efficiency in the multiplication of this hybrid clone using TIB®.
publishDate 2018
dc.date.none.fl_str_mv 2018
2019-05-14T12:17:25Z
2019-05-14T12:17:25Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv MÁXIMO, W. P. F. et al. In vitro multiplication of Eucalyptus hybrid via temporary immersion bioreactor: culture media and cytokinin effects. Crop Breeding and Applied Biotechnology, Viçosa, MG, v. 18, n. 2, Apr./June 2018.
http://repositorio.ufla.br/jspui/handle/1/34267
identifier_str_mv MÁXIMO, W. P. F. et al. In vitro multiplication of Eucalyptus hybrid via temporary immersion bioreactor: culture media and cytokinin effects. Crop Breeding and Applied Biotechnology, Viçosa, MG, v. 18, n. 2, Apr./June 2018.
url http://repositorio.ufla.br/jspui/handle/1/34267
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
dc.source.none.fl_str_mv Crop Breeding and Applied Biotechnology
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
_version_ 1807835198874714112

Registros relacionados