Enzymatic and antagonistic potential of bacteria isolated from typical fruit of Cerrado in Minas Gerais State, Brazil

Detalhes bibliográficos
Autor(a) principal: Silva, Cristina Ferreira
Data de Publicação: 2015
Outros Autores: Reis, Kelly Cristina dos, Lopes, Noelly Alves, Dias, Mariana, Batista, Luis Roberto, Schwan, Rosane Freitas
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/13459
Resumo: Brazil has great biodiversity, which is observed in the Cerrado biome of the tropical Brazilian savanna. The objectives of this study were to isolate and identify bacteria from Psychotria hoffmannseggiana with potential cellulase and pectinase production and with antagonistic activity against Aspergillus carbonarius, an Ochratoxin A (OTA) producer. Ripe fruit were collected in the region of Passos City in the preserved Cerrado area in Minas Gerais State, Brazil. Serial dilutions were performed, and the bacteria isolated were biochemically characterized and identified by sequencing. To analyze the production of enzymes, the bacteria were cultivated in CMC and pectinase media. The better enzyme producers were optimized for production. Assays on the antagonistic activity for growth and sporulation were carried out in co-culture (bacteria and filamentous fungi). TLC was performed to verify the mycotoxin production. The predominant microbiota were Gram-negative bacteria belonging to the Enterobacteriaceae family. Some isolates showed potential for enzymatic and antagonistic activity, especially the isolate identified as Lysinibacillus fusiformis. This species was a better producer of cellulases (maximum activity: 103.1 mg glucose min.-1 mg-1 protein). In conclusion, the bacteria isolated from Psychotria hoffmannseggiana showed biotechnological potential for agro-industry and the environmental aspect.
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spelling Enzymatic and antagonistic potential of bacteria isolated from typical fruit of Cerrado in Minas Gerais State, BrazilPotencial enzimático e antagonista de bactérias isoladas de fruto típico do Cerrado em Minas Gerais, BrasilBactérias celulolíticasBactérias pectinolíticasProdução enzimática - OtimizaçãoCellulolytic bacteriaPectinolytic bacteriaEnzymatic Production - OptimizationBrazil has great biodiversity, which is observed in the Cerrado biome of the tropical Brazilian savanna. The objectives of this study were to isolate and identify bacteria from Psychotria hoffmannseggiana with potential cellulase and pectinase production and with antagonistic activity against Aspergillus carbonarius, an Ochratoxin A (OTA) producer. Ripe fruit were collected in the region of Passos City in the preserved Cerrado area in Minas Gerais State, Brazil. Serial dilutions were performed, and the bacteria isolated were biochemically characterized and identified by sequencing. To analyze the production of enzymes, the bacteria were cultivated in CMC and pectinase media. The better enzyme producers were optimized for production. Assays on the antagonistic activity for growth and sporulation were carried out in co-culture (bacteria and filamentous fungi). TLC was performed to verify the mycotoxin production. The predominant microbiota were Gram-negative bacteria belonging to the Enterobacteriaceae family. Some isolates showed potential for enzymatic and antagonistic activity, especially the isolate identified as Lysinibacillus fusiformis. This species was a better producer of cellulases (maximum activity: 103.1 mg glucose min.-1 mg-1 protein). In conclusion, the bacteria isolated from Psychotria hoffmannseggiana showed biotechnological potential for agro-industry and the environmental aspect.O Brasil tem uma grande biodiversidade no bioma Cerrado típico de savanas tropicais. Os objetivos deste estudo foram isolar e identificar bactérias celulolíticas e pectinolíticas e com atividade antagonista contra contra Aspergillus. carbonarius produtores de ocratoxina a partir de Psychotria hoffmannseggiana. Frutos maduros de P. hoffmannseggianaforam coletadas na região da cidade de Passos em área preservada de Cerrado em Minas Gerais, Brasil. Realizou-se diluições decimais e as bactérias isoladas foram caracterizados bioquimicamente e identificadas por seqüenciamento. Para a produção de enzimas, as bactérias foram cultivadas em CMC e MP5 e MP7. Os isolados com melhor produção enzimática foram submetidos ao processo de otimização. A atividade antagonista foi realizada em co-cultura (bactérias e fungos filamentosos) e avaliou-se o crescimento e a esporulação. TLC foram realizados para verificar a produção de micotoxinas. A microbiota predominante foi Gram negativas pertencem à família Enterobacteriaceae. Alguns isolados apresentaram potencial para a atividade enzimática e antagonista, especialmente o isolado identificado como Lysinibacillus fusiformis. Esta espécie foi melhor produtora de celulases (atividade máxima 103,1 mg glicose min.-1 mg-1 de proteína). Três dos 105 isolados de bactérias estimularam a produção de micotoxinas. Conclui-se que estes isolados apresentam potencial biotecnológico para a área de agro-indústria e meio ambiente.Editora da Universidade Estadual de Maringá2017-08-01T17:11:47Z2017-08-01T17:11:47Z2015-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfSILVA, C. F. et al. Enzymatic and antagonistic potential of bacteria isolated from typical fruit of Cerrado in Minas Gerais State, Brazil. Acta Scientiarum. Agronomy, Maringá, v. 37, n. 3, p. 367-374, July/Sept. 2015.http://repositorio.ufla.br/jspui/handle/1/13459Acta Scientiarum. Agronomyreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLASilva, Cristina FerreiraReis, Kelly Cristina dosLopes, Noelly AlvesDias, MarianaBatista, Luis RobertoSchwan, Rosane Freitasinfo:eu-repo/semantics/openAccesseng2017-08-01T17:23:21Zoai:localhost:1/13459Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2017-08-01T17:23:21Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Enzymatic and antagonistic potential of bacteria isolated from typical fruit of Cerrado in Minas Gerais State, Brazil
Potencial enzimático e antagonista de bactérias isoladas de fruto típico do Cerrado em Minas Gerais, Brasil
title Enzymatic and antagonistic potential of bacteria isolated from typical fruit of Cerrado in Minas Gerais State, Brazil
spellingShingle Enzymatic and antagonistic potential of bacteria isolated from typical fruit of Cerrado in Minas Gerais State, Brazil
Silva, Cristina Ferreira
Bactérias celulolíticas
Bactérias pectinolíticas
Produção enzimática - Otimização
Cellulolytic bacteria
Pectinolytic bacteria
Enzymatic Production - Optimization
title_short Enzymatic and antagonistic potential of bacteria isolated from typical fruit of Cerrado in Minas Gerais State, Brazil
title_full Enzymatic and antagonistic potential of bacteria isolated from typical fruit of Cerrado in Minas Gerais State, Brazil
title_fullStr Enzymatic and antagonistic potential of bacteria isolated from typical fruit of Cerrado in Minas Gerais State, Brazil
title_full_unstemmed Enzymatic and antagonistic potential of bacteria isolated from typical fruit of Cerrado in Minas Gerais State, Brazil
title_sort Enzymatic and antagonistic potential of bacteria isolated from typical fruit of Cerrado in Minas Gerais State, Brazil
author Silva, Cristina Ferreira
author_facet Silva, Cristina Ferreira
Reis, Kelly Cristina dos
Lopes, Noelly Alves
Dias, Mariana
Batista, Luis Roberto
Schwan, Rosane Freitas
author_role author
author2 Reis, Kelly Cristina dos
Lopes, Noelly Alves
Dias, Mariana
Batista, Luis Roberto
Schwan, Rosane Freitas
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Silva, Cristina Ferreira
Reis, Kelly Cristina dos
Lopes, Noelly Alves
Dias, Mariana
Batista, Luis Roberto
Schwan, Rosane Freitas
dc.subject.por.fl_str_mv Bactérias celulolíticas
Bactérias pectinolíticas
Produção enzimática - Otimização
Cellulolytic bacteria
Pectinolytic bacteria
Enzymatic Production - Optimization
topic Bactérias celulolíticas
Bactérias pectinolíticas
Produção enzimática - Otimização
Cellulolytic bacteria
Pectinolytic bacteria
Enzymatic Production - Optimization
description Brazil has great biodiversity, which is observed in the Cerrado biome of the tropical Brazilian savanna. The objectives of this study were to isolate and identify bacteria from Psychotria hoffmannseggiana with potential cellulase and pectinase production and with antagonistic activity against Aspergillus carbonarius, an Ochratoxin A (OTA) producer. Ripe fruit were collected in the region of Passos City in the preserved Cerrado area in Minas Gerais State, Brazil. Serial dilutions were performed, and the bacteria isolated were biochemically characterized and identified by sequencing. To analyze the production of enzymes, the bacteria were cultivated in CMC and pectinase media. The better enzyme producers were optimized for production. Assays on the antagonistic activity for growth and sporulation were carried out in co-culture (bacteria and filamentous fungi). TLC was performed to verify the mycotoxin production. The predominant microbiota were Gram-negative bacteria belonging to the Enterobacteriaceae family. Some isolates showed potential for enzymatic and antagonistic activity, especially the isolate identified as Lysinibacillus fusiformis. This species was a better producer of cellulases (maximum activity: 103.1 mg glucose min.-1 mg-1 protein). In conclusion, the bacteria isolated from Psychotria hoffmannseggiana showed biotechnological potential for agro-industry and the environmental aspect.
publishDate 2015
dc.date.none.fl_str_mv 2015-07
2017-08-01T17:11:47Z
2017-08-01T17:11:47Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv SILVA, C. F. et al. Enzymatic and antagonistic potential of bacteria isolated from typical fruit of Cerrado in Minas Gerais State, Brazil. Acta Scientiarum. Agronomy, Maringá, v. 37, n. 3, p. 367-374, July/Sept. 2015.
http://repositorio.ufla.br/jspui/handle/1/13459
identifier_str_mv SILVA, C. F. et al. Enzymatic and antagonistic potential of bacteria isolated from typical fruit of Cerrado in Minas Gerais State, Brazil. Acta Scientiarum. Agronomy, Maringá, v. 37, n. 3, p. 367-374, July/Sept. 2015.
url http://repositorio.ufla.br/jspui/handle/1/13459
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Editora da Universidade Estadual de Maringá
publisher.none.fl_str_mv Editora da Universidade Estadual de Maringá
dc.source.none.fl_str_mv Acta Scientiarum. Agronomy
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
_version_ 1807835097245679616

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