Propagação in vitro de copo-de-leite: sulfato de adenina e 6-benzilaminopurina

Detalhes bibliográficos
Autor(a) principal: Ribeiro, Márcia de Nazaré Oliveira
Data de Publicação: 2014
Outros Autores: Pasqual, Moacir, Silva, Adriano Bortolotti da, Rodrigues, Vantuil Antônio
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/38383
Resumo: Calla lily [Zantedeschia aethiopica (L.) Spreng.] belonging to the Araceae family is appreciated as cut flower and in com­position of gardens. However, the conventional propagation of this plants shows a poor productive. Thus, tissue culture besides allowing fast clonal propagation also provides healthy and uniforms plants. The aim was study the influence of the differents concentrations of 6-benzilaminopurine (BAP) and adenine sulphate (AS) on in vitro multiplication of Calla lily. The explants were maintained in MS medium added with BAP (0.0, 8.9, 17.8 and 26.7 μM) and adenine sulphate (0, 54, 108 and 162 μM). The plants were transferred to growth room and maintained at 25±1ºC and photoperiod of 16 hours for 60 days, under luminous intensity of 35 μmol m-2 s-1, for a period of 60 days. The experimental design was entirely randomized with four repetitions of three seedlings each, resulting in twelve plants per treatment, each tube with one plant. The statistics analysis showed interactive effects for quantify of BAP and AS for leaves number and fresh mass of the aerial parts. The highest number of leaves (4.8) and fresh mass of aerial parts (0.73 g) was obtained with 26.7 μM of BAP combined with 108 μM of AS, highest number of shoots (2.6) was obtained with 22,19 μM of BAP and highest lengh of sprouts (5.0 cm) was observed in the absence of BAP. The addition of BAP increased the number of shoots per explant. The use of adenine sulphate in combination with BAP had a positive effect for the accumulation of fresh weight and number of leaves in vitro culture.
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spelling Propagação in vitro de copo-de-leite: sulfato de adenina e 6-benzilaminopurinaIn vitro propagation of Calla lily: adenine sulphate and 6-benzilaminopurineZantedeschia aethiopicaPlantas ornamentaisCultura de tecidosOrnamental plantsTissue cultureCalla lily [Zantedeschia aethiopica (L.) Spreng.] belonging to the Araceae family is appreciated as cut flower and in com­position of gardens. However, the conventional propagation of this plants shows a poor productive. Thus, tissue culture besides allowing fast clonal propagation also provides healthy and uniforms plants. The aim was study the influence of the differents concentrations of 6-benzilaminopurine (BAP) and adenine sulphate (AS) on in vitro multiplication of Calla lily. The explants were maintained in MS medium added with BAP (0.0, 8.9, 17.8 and 26.7 μM) and adenine sulphate (0, 54, 108 and 162 μM). The plants were transferred to growth room and maintained at 25±1ºC and photoperiod of 16 hours for 60 days, under luminous intensity of 35 μmol m-2 s-1, for a period of 60 days. The experimental design was entirely randomized with four repetitions of three seedlings each, resulting in twelve plants per treatment, each tube with one plant. The statistics analysis showed interactive effects for quantify of BAP and AS for leaves number and fresh mass of the aerial parts. The highest number of leaves (4.8) and fresh mass of aerial parts (0.73 g) was obtained with 26.7 μM of BAP combined with 108 μM of AS, highest number of shoots (2.6) was obtained with 22,19 μM of BAP and highest lengh of sprouts (5.0 cm) was observed in the absence of BAP. The addition of BAP increased the number of shoots per explant. The use of adenine sulphate in combination with BAP had a positive effect for the accumulation of fresh weight and number of leaves in vitro culture.O copo-de-leite [Zantedeschia aethiopica (L.) Spreng.], pertencente à família Araceae, é bastante apreciado tanto como flor de corte quanto na composição de jardins. Entretanto, a propagação convencional dessa espécie apresenta baixo rendimento. A cultura de tecidos, além de permitir rápida propagação clonal, também proporciona a obtenção de plantas uniformes e sadias. Sendo assim, o objetivo do trabalho foi estudar a influência de diferentes concentrações de 6-benzilaminopurina (BAP) e sulfato de adenina (SA) sobre o desenvolvimento in vitro de explantes de copo-de-leite. As culturas foram mantidas em meio MS acrescido de BAP (0,0; 8,9; 17,8 e 26,7 µM) e SA (0, 54, 108 e 162 µM) em arranjo fatorial (4x4). O material vegetal foi cultivado em sala de crescimento com temperatura de 27 ± 1ºC, fotoperíodo de 16 horas, sob intensidade luminosa de 35 μmol m-2 s-1, por período de 60 dias. O delineamento experimental utilizado foi inteiramente casualizado, com quatro repetições, e a unidade experimental composta de três tubos de ensaio, sendo cada tubo constituído por um explante. A interação significativa entre os fatores BAP e SA ocorreu para número de folhas por explante (4,8) e massa fresca da parte aérea (0,73 g) com 26,7 µM de BAP e 108 µM de SA. Para número de brotos obtidos por explante (2,6) apenas o BAP apresentou significância na concentração de 22,19 µM. Os brotos apresentaram maior comprimento (5,0 cm) na ausência de BAP. A adição de BAP incrementou o número de brotos por explante. O emprego de sulfato de adenina em combinação com BAP teve efeito positivo para o acumulo de massa fresca e numero de folhas no cultivo in vitro. Palavras-chave: Zantedeschia aethiopica, plantas ornamentais, cultura de tecidos.Sociedade Brasileira de Floricultura e Plantas Ornamentais (SBFPO)2019-12-30T18:29:22Z2019-12-30T18:29:22Z2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfRIBEIRO, M. de N. O. et al. Propagação in vitro de copo-de-leite: sulfato de adenina e 6-benzilaminopurina. Revista Brasileira de Horticultura Ornamental, [S.l.], v. 20, n. 1, p. 21-26, 2014. DOI: 10.14295/rbho.v20i1.660.http://repositorio.ufla.br/jspui/handle/1/38383Revista Brasileira de Horticultura Ornamentalreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessRibeiro, Márcia de Nazaré OliveiraPasqual, MoacirSilva, Adriano Bortolotti daRodrigues, Vantuil Antôniopor2019-12-30T18:29:22Zoai:localhost:1/38383Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2019-12-30T18:29:22Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Propagação in vitro de copo-de-leite: sulfato de adenina e 6-benzilaminopurina
In vitro propagation of Calla lily: adenine sulphate and 6-benzilaminopurine
title Propagação in vitro de copo-de-leite: sulfato de adenina e 6-benzilaminopurina
spellingShingle Propagação in vitro de copo-de-leite: sulfato de adenina e 6-benzilaminopurina
Ribeiro, Márcia de Nazaré Oliveira
Zantedeschia aethiopica
Plantas ornamentais
Cultura de tecidos
Ornamental plants
Tissue culture
title_short Propagação in vitro de copo-de-leite: sulfato de adenina e 6-benzilaminopurina
title_full Propagação in vitro de copo-de-leite: sulfato de adenina e 6-benzilaminopurina
title_fullStr Propagação in vitro de copo-de-leite: sulfato de adenina e 6-benzilaminopurina
title_full_unstemmed Propagação in vitro de copo-de-leite: sulfato de adenina e 6-benzilaminopurina
title_sort Propagação in vitro de copo-de-leite: sulfato de adenina e 6-benzilaminopurina
author Ribeiro, Márcia de Nazaré Oliveira
author_facet Ribeiro, Márcia de Nazaré Oliveira
Pasqual, Moacir
Silva, Adriano Bortolotti da
Rodrigues, Vantuil Antônio
author_role author
author2 Pasqual, Moacir
Silva, Adriano Bortolotti da
Rodrigues, Vantuil Antônio
author2_role author
author
author
dc.contributor.author.fl_str_mv Ribeiro, Márcia de Nazaré Oliveira
Pasqual, Moacir
Silva, Adriano Bortolotti da
Rodrigues, Vantuil Antônio
dc.subject.por.fl_str_mv Zantedeschia aethiopica
Plantas ornamentais
Cultura de tecidos
Ornamental plants
Tissue culture
topic Zantedeschia aethiopica
Plantas ornamentais
Cultura de tecidos
Ornamental plants
Tissue culture
description Calla lily [Zantedeschia aethiopica (L.) Spreng.] belonging to the Araceae family is appreciated as cut flower and in com­position of gardens. However, the conventional propagation of this plants shows a poor productive. Thus, tissue culture besides allowing fast clonal propagation also provides healthy and uniforms plants. The aim was study the influence of the differents concentrations of 6-benzilaminopurine (BAP) and adenine sulphate (AS) on in vitro multiplication of Calla lily. The explants were maintained in MS medium added with BAP (0.0, 8.9, 17.8 and 26.7 μM) and adenine sulphate (0, 54, 108 and 162 μM). The plants were transferred to growth room and maintained at 25±1ºC and photoperiod of 16 hours for 60 days, under luminous intensity of 35 μmol m-2 s-1, for a period of 60 days. The experimental design was entirely randomized with four repetitions of three seedlings each, resulting in twelve plants per treatment, each tube with one plant. The statistics analysis showed interactive effects for quantify of BAP and AS for leaves number and fresh mass of the aerial parts. The highest number of leaves (4.8) and fresh mass of aerial parts (0.73 g) was obtained with 26.7 μM of BAP combined with 108 μM of AS, highest number of shoots (2.6) was obtained with 22,19 μM of BAP and highest lengh of sprouts (5.0 cm) was observed in the absence of BAP. The addition of BAP increased the number of shoots per explant. The use of adenine sulphate in combination with BAP had a positive effect for the accumulation of fresh weight and number of leaves in vitro culture.
publishDate 2014
dc.date.none.fl_str_mv 2014
2019-12-30T18:29:22Z
2019-12-30T18:29:22Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv RIBEIRO, M. de N. O. et al. Propagação in vitro de copo-de-leite: sulfato de adenina e 6-benzilaminopurina. Revista Brasileira de Horticultura Ornamental, [S.l.], v. 20, n. 1, p. 21-26, 2014. DOI: 10.14295/rbho.v20i1.660.
http://repositorio.ufla.br/jspui/handle/1/38383
identifier_str_mv RIBEIRO, M. de N. O. et al. Propagação in vitro de copo-de-leite: sulfato de adenina e 6-benzilaminopurina. Revista Brasileira de Horticultura Ornamental, [S.l.], v. 20, n. 1, p. 21-26, 2014. DOI: 10.14295/rbho.v20i1.660.
url http://repositorio.ufla.br/jspui/handle/1/38383
dc.language.iso.fl_str_mv por
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dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Floricultura e Plantas Ornamentais (SBFPO)
publisher.none.fl_str_mv Sociedade Brasileira de Floricultura e Plantas Ornamentais (SBFPO)
dc.source.none.fl_str_mv Revista Brasileira de Horticultura Ornamental
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
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instname_str Universidade Federal de Lavras (UFLA)
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institution UFLA
reponame_str Repositório Institucional da UFLA
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repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
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