Crescimento in vitro de Pogostemon cablin sob intensidades luminosas e leds
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/11363 |
Resumo: | Pogostemon cablin (Blanco) Benth, commonly known as patchouli, is an aromatic species of great commercial value, especially for perfumeries, due to the essential oil extracted from its leaves. However, the propagation of this species under field conditions suffers losses due to the incidence of diseases and to the lack of knowledge of technical factors that affect its cultivation. Therefore, tissue culture is a technique that can benefit the production chain of this species. However, for its application on plant propagation, it is necessary to adapt factors such as cultivation medium, light regime, light quality and intensity, etc. With this work, we aimed at evaluating the influence of light quality and intensity over the growth of patchouli plantlets cultivated in vitro, submitted to different intensities and light spectrums. The explants were cultivated under the light intensities of 28, 51, 64, 76 and 113 µmol m -2 s -1 , with four replicates each. For light quality, we used LED lamps, with six treatments in the colors white, red, blue, blue and red (1:3), blue and red (3:1), and blue and red (1:1), with four replicates. After 40 days, growth and photosynthetic pigment analyses were performed. The results showed that light intensity and quality influenced the growth of P. cablin. The treatment with 76 µmol m -2 s -1 obtained the best results for number of leaves, root length, root dry biomass and total dry biomass. For the photosynthetic pigments, the highest contents were verified with 51 µmol m -2 s -1 . The LEDs in the combinations of blue and red (1:3) and white spectrums obtained the best growth values. It is necessary to evaluate the influence of this light regime over volatile chemical composition of plantlets cultivated in vitro and acclimatized in order to establish a micropropagation method for this species. |
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Crescimento in vitro de Pogostemon cablin sob intensidades luminosas e ledsGrowth in vitro pogostemon cablin under light intensities and leds att. carla resuenoPatchouliPlanta aromáticaPlantas - Efeito da luzPlantas - Cultura in vitroMicropropagação de plantasAromatic plantPlants - Effect of lightPlants - In vitro cultureMicropropagation of plantsFitotecniaPogostemon cablin (Blanco) Benth, commonly known as patchouli, is an aromatic species of great commercial value, especially for perfumeries, due to the essential oil extracted from its leaves. However, the propagation of this species under field conditions suffers losses due to the incidence of diseases and to the lack of knowledge of technical factors that affect its cultivation. Therefore, tissue culture is a technique that can benefit the production chain of this species. However, for its application on plant propagation, it is necessary to adapt factors such as cultivation medium, light regime, light quality and intensity, etc. With this work, we aimed at evaluating the influence of light quality and intensity over the growth of patchouli plantlets cultivated in vitro, submitted to different intensities and light spectrums. The explants were cultivated under the light intensities of 28, 51, 64, 76 and 113 µmol m -2 s -1 , with four replicates each. For light quality, we used LED lamps, with six treatments in the colors white, red, blue, blue and red (1:3), blue and red (3:1), and blue and red (1:1), with four replicates. After 40 days, growth and photosynthetic pigment analyses were performed. The results showed that light intensity and quality influenced the growth of P. cablin. The treatment with 76 µmol m -2 s -1 obtained the best results for number of leaves, root length, root dry biomass and total dry biomass. For the photosynthetic pigments, the highest contents were verified with 51 µmol m -2 s -1 . The LEDs in the combinations of blue and red (1:3) and white spectrums obtained the best growth values. It is necessary to evaluate the influence of this light regime over volatile chemical composition of plantlets cultivated in vitro and acclimatized in order to establish a micropropagation method for this species.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Pogostemon cablin (Blanco) Benth, popularmente conhecida como patchouli, é uma espécie aromática, que possui grande valor comercial, especialmente, na perfumaria pelo óleo essencial extraído de suas folhas. Todavia a propagação dessa espécie em condições de campo sofre grandes perdas em função das incidências de doenças e falta de conhecimento de fatores técnicos para seu cultivo. Assim a cultura de tecidos pode ser uma técnica que beneficie a cadeia produtiva dessa espécie. Porém, para sua aplicação na propagação de plantas, é necessário adequar fatores como o meio de cultivo, regime de luz, qualidade e intensidade de luz, dentre outros. Objetivou-se avaliar a influência da intensidade e qualidade luminosa no crescimento de plântulas de patchouli cultivadas in vitro, submetidas a diferentes intensidades e espectros de luz. Os explantes foram cultivados sob as intensidades luminosas 28, 51, 64, 76 e 113 µmol m -2 s -1 , com 4 repetições cada. Para qualidade luminosa, foram usados lâmpadas de LEDs, com 6 tratamentos, LEDs de cor branca, vermelha, azul, azul e vermelha (1:3), azul e vermelha azul (3:1) e vermelha e azul (1:1), com 4 repetições. Após 40 dias foram feitas as avaliações de crescimento e pigmentos fotossintéticos. Os resultados observados mostram que a intensidade e qualidade luminosa influenciaram o crescimento de P. Cablin. O tratamento com 76 µmol m -2 s -1 obteve melhores resultados para número de folhas, comprimento de raiz, biomassa seca de raiz e biomassa seca total. Para os pigmentos fotossintéticos os maiores teores foram observados com 51 µmol m -2 s -1 . Os LEDs nas combinações de espectros azul e vermelho (1:3) e branca obtiveram os melhores valores de crescimento. Faz-se necessário avaliar, ainda, a influência desses regimes luminosos, na composição química volátil de plântulas in vitro e aclimatizadas, para que possa ser estabelecido um método de micropropagação da espécie.Universidade Federal de LavrasPrograma de de Pós-Graduação em Plantas Medicinais, Aromáticas e CondimentaresUFLAbrasilDepartamento de AgriculturaBertolucci, Suzan Kelly VilelaPinto, José Eduardo Brasil PereiraPinto, José Eduardo Brasil PereiraCorrêa, Ricardo MonteiroPacheco, Fernanda VentorimCoelho, Carla Carolynne Resueno2016-07-15T14:28:31Z2016-07-15T14:28:31Z2016-07-152016-04-20info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfCOELHO, C. C. R. Crescimento in vitro de Pogostemon cablin sob intensidades luminosas e leds. 2016. 69 p. Dissertação (Mestrado em Plantas Medicinais, Aromáticas e Condimentares)-Universidade Federal de Lavras, Lavras, 2016.http://repositorio.ufla.br/jspui/handle/1/11363porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLA2016-07-15T14:28:31Zoai:localhost:1/11363Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2016-07-15T14:28:31Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Crescimento in vitro de Pogostemon cablin sob intensidades luminosas e leds Growth in vitro pogostemon cablin under light intensities and leds att. carla resueno |
title |
Crescimento in vitro de Pogostemon cablin sob intensidades luminosas e leds |
spellingShingle |
Crescimento in vitro de Pogostemon cablin sob intensidades luminosas e leds Coelho, Carla Carolynne Resueno Patchouli Planta aromática Plantas - Efeito da luz Plantas - Cultura in vitro Micropropagação de plantas Aromatic plant Plants - Effect of light Plants - In vitro culture Micropropagation of plants Fitotecnia |
title_short |
Crescimento in vitro de Pogostemon cablin sob intensidades luminosas e leds |
title_full |
Crescimento in vitro de Pogostemon cablin sob intensidades luminosas e leds |
title_fullStr |
Crescimento in vitro de Pogostemon cablin sob intensidades luminosas e leds |
title_full_unstemmed |
Crescimento in vitro de Pogostemon cablin sob intensidades luminosas e leds |
title_sort |
Crescimento in vitro de Pogostemon cablin sob intensidades luminosas e leds |
author |
Coelho, Carla Carolynne Resueno |
author_facet |
Coelho, Carla Carolynne Resueno |
author_role |
author |
dc.contributor.none.fl_str_mv |
Bertolucci, Suzan Kelly Vilela Pinto, José Eduardo Brasil Pereira Pinto, José Eduardo Brasil Pereira Corrêa, Ricardo Monteiro Pacheco, Fernanda Ventorim |
dc.contributor.author.fl_str_mv |
Coelho, Carla Carolynne Resueno |
dc.subject.por.fl_str_mv |
Patchouli Planta aromática Plantas - Efeito da luz Plantas - Cultura in vitro Micropropagação de plantas Aromatic plant Plants - Effect of light Plants - In vitro culture Micropropagation of plants Fitotecnia |
topic |
Patchouli Planta aromática Plantas - Efeito da luz Plantas - Cultura in vitro Micropropagação de plantas Aromatic plant Plants - Effect of light Plants - In vitro culture Micropropagation of plants Fitotecnia |
description |
Pogostemon cablin (Blanco) Benth, commonly known as patchouli, is an aromatic species of great commercial value, especially for perfumeries, due to the essential oil extracted from its leaves. However, the propagation of this species under field conditions suffers losses due to the incidence of diseases and to the lack of knowledge of technical factors that affect its cultivation. Therefore, tissue culture is a technique that can benefit the production chain of this species. However, for its application on plant propagation, it is necessary to adapt factors such as cultivation medium, light regime, light quality and intensity, etc. With this work, we aimed at evaluating the influence of light quality and intensity over the growth of patchouli plantlets cultivated in vitro, submitted to different intensities and light spectrums. The explants were cultivated under the light intensities of 28, 51, 64, 76 and 113 µmol m -2 s -1 , with four replicates each. For light quality, we used LED lamps, with six treatments in the colors white, red, blue, blue and red (1:3), blue and red (3:1), and blue and red (1:1), with four replicates. After 40 days, growth and photosynthetic pigment analyses were performed. The results showed that light intensity and quality influenced the growth of P. cablin. The treatment with 76 µmol m -2 s -1 obtained the best results for number of leaves, root length, root dry biomass and total dry biomass. For the photosynthetic pigments, the highest contents were verified with 51 µmol m -2 s -1 . The LEDs in the combinations of blue and red (1:3) and white spectrums obtained the best growth values. It is necessary to evaluate the influence of this light regime over volatile chemical composition of plantlets cultivated in vitro and acclimatized in order to establish a micropropagation method for this species. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-07-15T14:28:31Z 2016-07-15T14:28:31Z 2016-07-15 2016-04-20 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
COELHO, C. C. R. Crescimento in vitro de Pogostemon cablin sob intensidades luminosas e leds. 2016. 69 p. Dissertação (Mestrado em Plantas Medicinais, Aromáticas e Condimentares)-Universidade Federal de Lavras, Lavras, 2016. http://repositorio.ufla.br/jspui/handle/1/11363 |
identifier_str_mv |
COELHO, C. C. R. Crescimento in vitro de Pogostemon cablin sob intensidades luminosas e leds. 2016. 69 p. Dissertação (Mestrado em Plantas Medicinais, Aromáticas e Condimentares)-Universidade Federal de Lavras, Lavras, 2016. |
url |
http://repositorio.ufla.br/jspui/handle/1/11363 |
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por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Lavras Programa de de Pós-Graduação em Plantas Medicinais, Aromáticas e Condimentares UFLA brasil Departamento de Agricultura |
publisher.none.fl_str_mv |
Universidade Federal de Lavras Programa de de Pós-Graduação em Plantas Medicinais, Aromáticas e Condimentares UFLA brasil Departamento de Agricultura |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
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UFLA |
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UFLA |
reponame_str |
Repositório Institucional da UFLA |
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Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
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1807835053688881152 |